• Journal of Applied Biological Chemistry
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• v.51 no.3
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• pp.88-94
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• 2008

Three different protein extraction methods-phenol extraction, trichloroacetic acid (TCA) precipitation, and desalting/TCA precipitation-were compared to determine the optimal reproducible high resolution 2-dimensional (2-D) electrophoresis for each chungkugjang, doenjang, and kochujang samples. The soluble proteins from Chungkugjang extracted by phenol were separated with high reproducibility and resolution, and gained 1.75- to 3-fold more protein spots on 2-D gel than those from the other methods. On the contrary, the extracted proteins from doenjang and kochujang treated by desalting/TCA precipitation method showed about 1.5- to 3.3-fold more protein spots on 2-D gel. Using the established methods, the changes in the protein profiles of the fermented soy foods were monitored during the fermentation period by 2-DE. One of the major proteins in soy, $\beta$-conglycinin $\alpha$-subuint, and some proteins with unknown functions were localized on 2-D gel as the protease-resistant proteins throughout the fermentation period of doenjang. Changes in the protein profile monitored by the established methods can provide basic information on unfolding the mechanisms of the generation of biofunctional activity in the fermented soy foods.

• Nuclear Engineering and Technology
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• v.37 no.3
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• pp.265-272
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• 2005

Severe accidents in nuclear power plants can cause hydrogen-generating chemical reactions, which create the danger of hydrogen combustion and thus threaten containment integrity. For containment analyses, a three-dimensional mechanistic code, GOTHIC-3D has been applied near source compartments to predict whether or not highly reactive gas mixtures can form during an accident with the hydrogen mitigation system working. To assess the code applicability to hydrogen combustion analysis, this paper presents the numerical calculation results of GOTHIC-3D for various hydrogen combustion experiments, including FLAME, LSVCTF, and SNU-2D. In this study, a technical base for the modeling oflarge- and small-scale facilities was introduced through sensitivity studies on cell size and bum modeling parameters. Use of a turbulent bum option of the eddy dissipation concept enabled scale-free applications. Lowering the bum parameter values for the flame thickness and the bum temperature limit resulted in a larger flame velocity. When applied to hydrogen combustion analysis, this study revealed that the GOTHIC-3D code is generally able to predict the combustion phenomena with its default bum modeling parameters for large-scale facilities. However, the code needs further modifications of its bum modeling parameters to be applied to either small-scale facilities or extremely fast transients.

• Natural Product Sciences
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• v.16 no.4
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• pp.207-210
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• 2010

Phytochemical investigation of Salvia plebeia resulted in the isolation of nine compounds. Their structures were determined to be 6-methoxynaringenin (1), 6-methoxynaringenin-7-O-$\beta$-D-glucoside (2), hispidulin (3), homoplantaginin (4), nepetin (5), nepitrin (6), 6-hydroxyluteolin (7), caffeic acid (8) and rosmarinic acid (9) by spectroscopic analyses. 6-Methoxynaringenin (1), 6-hydroxyluteolin (7) and rosmarinic acid (9) were isolated from this plant for the first time.

• Computers and Concrete
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• v.4 no.2
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• pp.135-156
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• 2007

Three 3D nonlinear finite-element models are developed to study the behavior of concrete beams and plates with and without external reinforcement by fibre-reinforced plastic (FRP). All three models are formulated based upon the 3D theory of elasticity. The stress model is modified from the element developed by Ramtekkar, et al. (2002) to incorporate material nonlinearity in the formulation. Both transverse stress and displacement components are used as nodal degrees-of-freedom to ensure the continuity of both stress and displacement components between the elements. The displacement model uses only displacement components as nodal degrees-of-freedom. The transition model has both stress and displacement components as nodal degrees-of-freedom on one surface, and only displacement components as nodal degrees-of-freedom on the opposite surface. The transition model serves as a connector between the stress and the displacement models. The developed models are validated by comparing the results of the analyses with an existing experimental result. Parametric studies of the effects of the externally reinforced FRP on the load capacity of reinforced concrete (RC) beams and concrete plates are performed to demonstrate the practicality and the efficiency of the proposed models.

• Korean Journal of Weed Science
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• v.15 no.1
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• pp.73-84
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• 1995

Four malformin A's produced by Aspergillus niger van Tiegh. were separated by HPLC equipped with $C_{18}$ reversed-phase column and subjected to structural determination. Amino acid analyses and mass spectra data of the compounds indicate that they structurally resemble the cyclic pentapeptide malformin $A_1$. Their structures were deduced by two dimensional NMR and MS/MS experiments as cyclo-D-Cys-D-Cys-L-Val-D-Leu-L-Ile for $A_1$, cyclo-D-Cys-D-Cys-L-Val-D-Leu-L-Val for $A_2$, cyclo-D-Cys-D-Cys-L-Val-D-Leu-L-Leu for $A_3$, and cyclo-D-Cys-D-Cys-L-Val-D-Ile-L-Val for $A_4$. Among the mal-formin A's, the structure of $A_3$ was identical to that of malformin C, which was produced by A. niger strain AN-1. All the malformin A's caused severe curvatures of corn(Zea mays L.) roots and the activities of the malformin A's with molecular weight 529 were greater than those with molecular weight 515. Malformin $A_1$ caused the corn root curvature by 83% at a concentration of $0.25{\mu}M$. In the mung bean(Phaseolus aureus Roxb.) hypercotyl segment test, however, the molecular weight of malformin A's was not a factor influencing the physiological activities. Malformin $A_1$ stimulated the growth of mung bean hypercotyles by 165% at a $0.1{\mu}M$ concentration.

• Spatial Information Research
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• v.20 no.4
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• pp.1-16
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• 2012

There is no doubt that the urban heat island (UHI) is a mounting problem in built-up environments, due to energy retention by the surface materials of dense buildings, leading to increased temperatures, air pollution, and energy consumption. To investigate the UHI, three-dimensional (3-D) information is necessary to analyze complex sites, including dense building clusters. In this research, 3-D building geometry information is combined with two-dimensional (2-D) urban surface information to examine the relationship between urban characteristics and temperature. In addition, this research introduces spatial regression models to account for the spatial spillover effects of urban temperatures, and includes the following steps: (a) estimating urban temperatures, (b) developing a 3-D city model, (c) generating urban parameters, and (d) conducting statistical analyses using both Ordinary Least-Squares (OLS) and Spatial Regression Models. The results demonstrate that 3-D urban characteristics greatly affect temperatures and that neighborhood effects are critical in explaining temperature variations. Finally, the implications of the results are discussed, providing guidelines for policies to reduce the UHI.

• Journal of computational fluids engineering
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• v.19 no.3
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• pp.85-90
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• 2014

The purpose of this study is to develop a new Meshless Method to solve 2-D compressible flow problems numerically. This paper includes a revised Least Square method that improves robustness compared with its original version by removing excessive numerical oscillation which occurs when points are randomly distributed. Numerical analyses of hypersonic flow over a blunt body were carried out using the method, then robustness, accuracy and convergence of their results were compared with those obtained from the original method.

• Korean Journal of Radiology
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• v.20 no.6
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• pp.880-893
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• 2019

Objective: To assess the technical performance of two-dimensional shear wave elastography (2D-SWE) for measuring liver stiffness. Materials and Methods: The Ovid-MEDLINE and EMBASE databases were searched for studies reporting the technical performance of 2D-SWE, including concerns with technical failures, unreliable measurements, interobserver reliability, and/or intraobserver reliability, published until June 30, 2018. The pooled proportion of technical failure and unreliable measurements was calculated using meta-analytic pooling via the random-effects model and inverse variance method for calculating weights. Subgroup analyses were performed to explore potential causes of heterogeneity. The pooled intraclass correlation coefficients (ICCs) for interobserver and intraobserver reliability were calculated using the Hedges-Olkin method with Fisher's Z transformation of the correlation coefficient. Results: The search yielded 34 articles. From 20 2D-SWE studies including 6196 patients, the pooled proportion of technical failure was 2.3% (95% confidence interval [CI], 1.3-3.9%). The pooled proportion of unreliable measurements from 20 studies including 6961 patients was 7.5% (95% CI, 4.7-11.7%). In the subgroup analyses, studies conducting more than three measurements showed fewer unreliable measurements than did those with three measurements or less, but no intergroup difference was found in technical failure. The pooled ICCs for interobserver reliability (from 10 studies including 517 patients) and intraobserver reliability (from 7 studies including 679 patients) were 0.87 (95% CI, 0.82-0.90) and 0.93 (95% CI, 0.89-0.95), respectively, suggesting good to excellent reliability. Conclusion: 2D-SWE shows good technical performance for assessing liver stiffness, with high technical success and reliability. Future studies should establish the quality criteria and optimal number of measurements.

• Bulletin of the Korean Chemical Society
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• v.34 no.12
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• pp.3749-3754
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• 2013

Two 3D isomorphous and isostructural complexes, namely, $[Zn(BDOA)(bpy)(H_2O)_2]_n$ (1) and $[Cd(BDOA)-(bpy)(H_2O)_2]_n$ (2); (BDOA = Benzene-1,4-dioxyacetic acid, bpy = 4,4'-bipyridine) were synthesized under hydrothermal conditions and characterized by means of elemental analyses, thermogravimetric (TG), infrared spectrometry, and single crystal X-ray diffraction. Complexes 1 and 2 crystallize in the triclinic system, space group P-1 and each metal ion in the complexes are six-coordinated with the same coordination environment. In the as-synthesized complexes, $BDOA^{2-}$ anions link central metal ions to form a 1D zigzag chain $[-BDOA^{2-}-Zn(Cd)-BDOA^{2-}-Zn(Cd)-]_{\infty}$, whereas bpy pillars connect metal ions to generate a 1D linear chain $[-bpy-Zn(Cd)-bpy-Zn(Cd)-]_{\infty}$. Both infinite chains are interweaved into 2D grid-like layers which are further constructed into a 3D open framework, where hydrogen bonds play as the bridges between the adjacent 2D layers. Luminescent properties of complex 1 showed selectivity for $Hg^{2+}$ ion.

• Spatial Information Research
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• v.17 no.2
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• pp.231-241
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• 2009

Recently, The importance of a quality control for implementing 3D geospatial database has being emphasized to build a 21st century knowledge society and an ubiquitous land. The improvement of 3D geospatial database quality control through establishing an integrated quality control makes data suppliers update data efficiently and users get high quality data. The purpose of this study was to derive the improvement plan of 3D geospatial database quality control through the analyses of the existing 3D geospatial database quality control and case studies. The results of this study are as follows. Firstly, we defined the concept of 3D geospatial database quality control. Secondly, we set the boundary, factors and process of 3D geospatial database quality control through classifying it in detail. Lastly, we drew improvement contents such as the quality control checklist by implementation process according to the improvement plan of 3D geospatial database implementation.