• Horticultural Science & Technology
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• v.32 no.5
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• pp.645-654
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• 2014

This study was conducted to investigate the effect of 1-methylcyclopropene (1-MCP, $1.0{\mu}L{\cdot}L^{-1}$), a known ethylene action inhibitor, on fruit quality and incidence of physiological disorders during a simulated marketing period at $25^{\circ}C$ for 20 days in early-season Asian pear (Pyrus pyrifolia Nakai) 'Hanareum' that had been treated with 0, 0.5, 1.2 or 2.4% $GA_{4+7}$. Weight loss of stored fruits increased with $GA_{4+7}$ concentration, and the 1-MCP treatment slightly reduced the weight loss rates during the marketing period. Flesh firmness decreased abruptly in all 1-MCP-untreated fruits as the storage period extended to 10 d, whereas the firmness of 1-MCP-treated fruits remained high (> 30 N) during 15 days shelf-life. The effect of 1-MCP was significantly reduced when fruits were subjected to increased GA concentration. Higher soluble solids content and acidity during extended shelf-life were also apparent in 1-MCP-treated 'Hanareum' pears. The L-values (lightness) and hue angles of 1-MCP treated samples were higher than those of controls during 20 days shelf-life, but the a-value (redness) was lower in 1-MCP treated fruits. 1-MCP treatment did not decrease the level of ethylene evolution regardless of $GA_{4+7}$ concentration during shelf-life in early-season Asian pear 'Hanareum'. By contrast, 1-MCP treatment decreased the respiration rate significantly during shelf-life. The efficacy of 1-MCP was greatest in the GA-untreated fruit and was reduced as the $GA_{4+7}$ concentration increased. 1-MCP treatment influenced the severity of physiological disorders including core browning and mealiness: 1-MCP treatment completely blocked the incidence of core browning of during 15 days shelf-life, and reduced the severity of mealiness during 20 days shelf-life regardless of $GA_{4+7}$ concentration. Based on our results, we conclude that the use of $1{\mu}L{\cdot}L^{-1}$ 1-MCP can be of great benefit for maintaining quality and preventing physiological disorders in early-season pear cultivar 'Hanareum' pear, whereas its efficacy decreases with the concentration of $GA_{4+7}$ whereas its efficacy gradually decreases when the concentration of $GA_{4+7}$ paste increased.

• Proceedings of the Korean Society of Postharvest Science and Technology of Agricultural Products Conference
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• 2003.10a
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• pp.186.2-187
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• 2003

에틸렌 작용억제제인 1-MCP(1-methylcyclopropene) 처리에 따른 토마토의 선도연장효과를 검정하기 위해 본 연구는 수행되었다. 토마토“슈퍼도태랑(Supermomotaro)”품종을 Mature green 단계에서 수화한 다음 1-MCP를 2$0^{\circ}C$에서 250, 500, 및 1000ppb 의 농도로 4시간 동안 처리후 온도 2$0^{\circ}C$, 90% RH 조건에 저장하면서 에틸렌 발생, 호흡량 및 품질특성 변화를 조사하였다. 토마토의 에틸렌 발생은 모든 처리구에서 저장 2일째 급등하는 경향을 보였는데, 1-MCP 처리구는 무처리에 비해 그 급등정도가 낮았으며, 호흡량 또한 1-MCP 처리에 의해 효과적으로 억제되었다. 특히 처리 2일 후부터 무처리에 비해 2배정도 낮은 호흡량을 나타냈다. 과피의 착색진행 정도를 Hunter color의 적색도“a”값으로 비교한 결과, 무처리는 2일부터 6일까지 빠르게 증가하였지만 1-MCP 처리구는 그 증가 속도가 낮았으며, 처리농도가 높을수록“a”값의 변화가 적은 경향을 보였다. 또한 황색도“b”값의 비교에 있어서도 1-MCP 처리구가 무처리에 비해 그 변화가 적었다. 경도는 무처리에서 2일 후에 급격히 감소하는데 비해 1-MCP처리에서는 4일까지 그 감소 폭이 적었으며, 1-MCP처리 농도별 차이는 크지 않았다. 부패과 발생은 무처리와 500ppb 처리에서 비슷한 수준이었으며, 1000ppb 처리에서는 오히려 무처리에 비해 부패율이 높아 고농도 처리에 의한 장해로 판단되었다. 그러나 250ppb 처리는 무처리에 비해 부패율이 적게 나타났다.

• Journal of Pharmaceutical Investigation
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• v.18 no.2
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• pp.43-47
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• 1988

In order to increase the dissolution characteristics of relatively water-insoluble metoclopramide (MCP), coprecipitates of MCP with polyvinylpyrrolidone (PVP), polyethylene glycol (PEG) 1000, 4000 or 6000 were prepared in various drug to polymer ratios. The dissolution rate of MCP-PVP coprecipitate was greater than those of MCP alone, MCP-PVP physical mixture and MCP-PEG coprecipitates. The dissolution rate of MCP-PEG 6000 coprecipitate was greater than those of MCP-PEG 1000 and MCP-PEG 4000 coprecipitates. The dissolution half-lives $(T_{50%})$ for MCP alone and 1:5 (w/w) MCP-PEG 6000 coprecipitate were determined by the log-probit method at $37^{\circ}C$ and found to be 4.17 and 0.98 min, respectively.

• Journal of Bio-Environment Control
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• v.25 no.2
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• pp.118-122
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• 2016

This study was conducted to identify the effects of 1-MCP and the storage methods for maintaining the quality of asparagus spears. Asparagus spears treated 1-MCP stored in MA condition and perforated film (conventional condition) and 1-MCP non-treated used as control. The treated asparagus was stored at $4^{\circ}C$ for 40 days. The fresh weight loss rate was less than 0.2% in all MA (Modified atmosphere) storage treatments, including the 1-MCP treatment, and 1-MCP shown the less fresh weight loss rate in perforated film storage after 20 days storage. The carbon dioxide concentration within a package was 5-12% in MA storage, which is whith the tolerated range of maximum $CO_2$ content of asparagus spears in recommended CA or MA conditions. The oxygen content was maintained between 5 to 15% in MAP treatments. The ethylene content was lowest at the MA storage with 1-MCP treatment. Visual quality, off-flavor, soluble solid contents, and hue angle on the final storage day were higher in MA storage and the 1-MCP treatment and conventional MA storage did not show any significant differences. These results suggest that the 1-MCP treatment did not significantly affected the quality and storability of asparagus spears.

• The Journal of Korean Institute of Information Technology
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• v.16 no.11
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• pp.43-49
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• 2018

Computer simulation was performed using the SIMION program to develop an electron gun for MCP cleaning. The target, MCP, is located 180mm from the source of the electron gun, and the diameter of the MCP is approximately 20mm. Therefore, we tried to find the condition that the beam diameter of electrons reaching the MCP is to be 20mm using four variables such as E, ${\phi}$, d1, d2, where the E is the energy of the electron reaching the MCP, the ${\phi}$ is the diameter of the extractor, and the d1 and the d2 are the distance from the electron source to the end of the extractor tube, and to the wall of chamber, respectively. As a result of simulation, we figuried out that the E and the d2 have little effect on the beam diameter. On the other hand, we also found that the beam diameters were very sensitive to the d1 and varied relatively large with respect to the ${\phi}$, and the d1 was the secondary order function of the ${\phi}$. Therefore we found that this function will allow us to design electron guns that are suitable for the purposes of this study.

• Journal of Embryo Transfer
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• v.32 no.3
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• pp.165-170
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• 2017

Pigs have been extensively used as mediators of xenotransplantation research. Specifically, the Massachusetts General Hospital (MGH) miniature pig was developed to fix major histocompatibility antigens for use in xenotransplantation studies. We generated transgenic pigs for xenotransplantation using MGH pigs. However, it has not been studied yet whether these pigs show similarity of reproductive physiological characteristics to wild types of MGH miniature pig. In this study we analyzed the estrous cycles and pregnancy characteristics of wild type (WT) and transgenic MGH miniature pigs, which were ${\alpha}1,3$-galactosyltransferase (GalT) heterozygous and homozygous knock-out, and membrane cofactor protein (MCP) inserted in its locus, $GalT^{-MCP/+}$ and $GalT^{-MCP/-MCP}$ pigs. Estrous cycles of WT, $GalT^{-MCP/+}$ and $GalT^{-MCP/-MCP}$ pigs were $20.9{\pm}0.74$, $20.1{\pm}1.26$, and $17.3{\pm}0.87days$, respectively, and periods of estrous were $3.2{\pm}0.10$, $3.1{\pm}0.12$, and $3.1{\pm}0.11days$. The periods of gestation of WT, $GalT^{-MCP/+}$ and $GalT^{-MCP/-MCP}$ pigs were $114.2{\pm}0.37$, $113.3{\pm}0.67$, and $115.4{\pm}0.51days$, respectively. Litter sizes of WT, $GalT^{-MCP/+}$ and $GalT^{-MCP/-MCP}$ pigs were $4.8{\pm}0.35$, $4.8{\pm}1.11$ and $3.0{\pm}0.32$ respectively. There were no significant differences on estrous cycle, periods of estrous and gestation, and litter size among WT, $GalT^{-MCP/+}$ and $GalT^{-MCP/-MCP}$ pigs, meaning that GalT knock-out and additional expression MCP of the MGH miniature pig did not effect on reproduction traits. These results provide relevant information to establish breeding system for MGH transgenic pig, and for propagation of $GalT^{-MCP/-MCP}$ pig to supply for xenotransplantation research.

• Journal of Ecology and Environment
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• v.29 no.3
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• pp.259-263
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• 2006

The objectives of this paper are to estimate home range and core habitat area of raccoon dog living in the rural area of Korea. A radio-telemetry study was carried out on 22 raccoon dog individuals. Among these individuals, 4 raccoon dogs made 2 pairs and they were monogamous and moved together all the year round. Mean home-range size of 9 individuals which were radio-tracked more than 3 months was $0.80km^2$ (100% MCP). The mean home range size of male individuals was $0.98km^2$ (N=5, 100% MCP) and that of female individuals was $0.58km^2$ (N=4, 100% MCP). On the other hand, in case 95% MCP(Mininlum Convex Polygon) was applied, the gap of home-range size between sex distinction was closed to $0.63km^2$ (male) and $0.42km^2$ (female). The home range size of two pairs of which the male and the female were radio-tracked at the same time showed little difference. In case of one pair, the home range size(95% MCP) was $0.28km^2$ (male) and $0.26km^2$ (female) and in case of the other pair, it was $0.36km^2$ each (male and female). Consequently there seems no significant difference in the home-range size between a male and a female racoon dog except the unusual cases such as unpaired individuals or the ones with no fixed territory.

• Journal of Bio-Environment Control
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• v.26 no.2
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• pp.108-114
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• 2017

The effect of 1-methylcyclopropene (1-MCP) at $1.0{\mu}L{\cdot}L^{-1}$ was compared with control and $10{\mu}L{\cdot}L^{-1}$ ethylene treatment to evaluate softening control of apple (Malus ${\times}$ domestica Borkh.) fruit for 180 days at $0.5^{\circ}C$ in the air, followed for 28 days at a room temperature. 1-MCP or 1-MCP+ethylene treatment maintained high fruit titratable acidity and firmness after 120 days during the cold storage, which was similarly observed for 28 days at a room temperature. 1-MCP treatment maintained fruit firmness more than 14 N during the cold storage and shelf-life at room temperature. Fruit surface red color was not consistently affected by the treatments during the cold storage but enhanced more than 4.0 by 1-MCP at 21- and 28-days of room temperature. Control or ethylene treatment advanced overall preceeding of fruit softening as rapid ethylene production and respiration rates at 90 days during the cold storage increased to a climacteric maximum. Therefore, pre 1-MCP-treated fruit maintained high fresh condition at a long-term low storage + approximately one month room temperature-storage under $10{\mu}L{\cdot}L^{-1}$ ethylene treatment.

• Journal of Radiation Industry
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• v.10 no.4
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• pp.205-210
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• 2016

This study was conducted to evaluate the combined effect of gamma irradiation and different conditions (vacuum packaging, antioxidant and freezing) on the microbiological and sensory characteristics of freeze dried Manila clam porridge (MCP) for immuno-compromised patient food. MCP can be sterilized at 1 kGy to 10 kGy. The initial counts of total aerobic bacteria and yeast molds in the non-irradiated MCP were $2.4{\pm}0.5$ and $1.2{\pm}0.3{\log}\;CFU\;g^{-1}$, respectively, but gamma irradiation significantly decreased the total aerobic bacteria to below the detection limit ($1{\log}\;CFU\;g^{-1}$) (5 kGy). Moreover, gamma irradiation effectively eliminated yeasts/molds at dose below than 1 kGy. However, gamma irradiation accelerated the increase of lipid oxidation and therefore, decreased the sensory characteristics of MCP as irradiation dose increased. To improve the sensory qualities of gamma irradiated MCP, combination treatment (vacuum packaging, 0.1% vitamin C) were applied. There was no significant difference in the overall acceptance scores between the combined-treatment sample (5.6 points) and the non-irradiated samples (6.0). The results indicate that combination treatment (vacuum packaging, 0.1% vitamin C) may help to maintain the quality of MCP. Therefore, it considered that irradiation of MCP with combined treatment and this is an effective method for the consumption as a special purpose food such as for space travel or immuno-compromised patients.

• Journal of Radiation Industry
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• v.4 no.3
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• pp.227-231
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• 2010

The purpose of this study was to investigate the expression mechanism of MCP-1 in gamma-irradiated RAW 264.7 macrophage cells. MCP-1 plays an important role in attracting monocyte to injured site at the early inflammation stage. However the production mechanism of MCP-1 by gamma-irradiation in RAW 264.7 macrophage cells was almost undiscovered. We found that MCP-1 was produced in RAW 264.7 macrophage cells by irradiation with 5 Gy. And these inceases were attenuated by specific inhibitors treatment, such as $NF-{\kappa}B$, JNK, ERK, JAK2, and Pyk2. These results indicate that radiation-induced MCP-1 production is mediated by MyD88- and TRIF-dependent pathways in RAW 264.7 macrophage cells. Furthermore, gamma-irradiation induced heme oxygenase-1 (HO-1) expression in RAW 264.7 macrophage cells. However this induction level was reduced before MCP-1 and $IFN-{\beta}$ production.