• Natural Product Sciences
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• 제4권2호
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• pp.53-57
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• 1998

Leaves of Crataegus sinaica Boiss, contain the new C-glycosyl flavone $3"',4”’-di-O-acetyl-2“-O-{\alpha}-rhamnosylvitexin$, together with the hitherto unknown, dihydroflavonol 3-O-xyloside, (2R:3R)-dihydroquercetin-$3-O-{\beta}-xylopyranoside$. The known compounds (+)-catechin, (-)-epicatechin, vicenin-II, $2"-O-{\alpha}-rhamnosylvitexin$, and $4"'-O-acetyl-2"-O-{\alpha}-rhamnosylvitexin$ were also characterized. Structures were established by conventional methods of analysis and confirmed by $^1H-,\;^{13}C-NMR$, and ESI-MS.

• Journal of the Korean Wood Science and Technology
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• 제47권1호
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• pp.1-7
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• 2019

Japanese anise (Illicium anisatum L.) leaves were collected and ground after drying, then immersed with 50 % aqueous acetone for 3 days. After filtration, the extracts were fractionated with n-hexane, chloroform ($CHCl_3$), ethylacetate (EtOAc) and $H_2O$, and then freeze dried after concentration. A portion of EtOAc (3.12 g) and $H_2O$ (6.08 g) soluble fractions were chromatographed on a Sephadex LH-20 column with various aqueous MeOH solution to isolate the compounds. Compound 1 ((+)-catechin) was isolated from EtOAc soluble fraction. Compounds 2 (quercetin), 3 (quercitrin) and 4 (2''-O-rhamnosylvitexin) were isolated from $H_2O$ soluble fraction. For the first time, quercitrin (3) and 2''-O-rhamnosylvitexin (4) of the isolated compounds were obtained from the extracts of japanese anise leaves.

• Archives of Pharmacal Research
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• 제17권5호
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• pp.314-317
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• 1994

The studies were camied out to evaluate the constituents in the leaves of Crataegus pinnatifida var. psilosa (Rosaceae) continuously. From the BuOH fraction of the MeOH extract, four flavonoid compounds, hyperoside (1), vitexin (2), 3"-O-rhamnosylvitexin (3) and $quercetin-3-O-{\alpha}-L-rhamnopyranoslyl-(1{\rightarrow}6)-{\beta}$-D-galactopyranoside (4) were isolated and identified on the basis of their physico-chemical properties and spectroscopic evidences by comparison with authentic samples.

• 생약학회지
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• 제47권3호
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• pp.222-225
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• 2016

Four flavone compounds were isolated from the whole plants of Setaria faberi Herrmann (Gramineae) through repeated column chromatography. Their chemical structures were elucidated as tricin (1), and three C-glycosylflavone, 2"-O-rhamnosylvitexin (2), 2"-O-rhamnosylscoparin (3), and 2"-O-rhamnosylorientin (4), respectively, by spectroscopic analysis. These compounds were isolated for the first time from this plant.

• 한국자원식물학회지
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• 제9권3호
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• pp.224-229
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• 1996

무궁화 품종 6계통, 총 68종류를 선발하여 flavonoid 성분을 비교하였다. 2차원으로 크로마토그래피를 전개 후 13개의 flavonoid가 관찰되었고 spot 5번의 flavonoid 함량이 전체의 50%를 차지하였으며 나머지는 미량으로 관찰되었다. 6개의 group별 spot출현 양상은 각각 차이를 보였으나 group 내에서는 거의 동일한 것으로 나타났다. 13개의 spot 중 1, 4, 6은 flavonol의 특성을 나타냈으며 나머지 10개의 spot들이 flavone의 특성을 나타내었다. 품종별 flavone함량 및 분포는 백색계통이나 자주 및 청색 계통에는 많은 양이 존재 하였고 pink와 적색 group에서는 매우 적은 양이 분포하였다. Purple spot들의 flavonoid aglycone은 4’, 5-OH의 aglycone 특성을 보였으며 이들 중 spot 5는 saponarin, 7은 vitexin, 9는 xylovitexin, 11은 rhamnosylvitexin으로 동정되었으며 13은 apigenin-7-O-diglycoside로 추정되었다. Saponarin은 백색무궁화에서 전체 flavone량의 50% 이상을 점유하였다.

• Journal of the Korean Wood Science and Technology
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• 제34권6호
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• pp.72-80
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• 2006

일본잎갈나무 낙엽(8.5 kg)을 채취하여 95% 에탄올 용액으로 추출하고 농축한 후 분획깔때기로 헥산, 메틸렌클로라이드, 에틸아세테이트 및 수용성으로 순차 추출하여 동결건조하였다. 에틸아세테이트용성과 수용성 분획에 대하여 칼럼크로마토그래피를 실시하였고, 충진물질로는 Sephadex LH-20을, 용리용매로는 메탄올 수용액 및 에탄올-헥산 혼합용액을 사용하였다. 단리된 화합물들은 TLC로 확인한 후 NMR 스펙트럼을 사용하여 구조규명을 하였고 FAB-MS와 EI-MS 스펙트럼으로 분자량을 측정하여 7개의 flavonoid 화합물인 (+)-catechin, (-)-epicatechin, 2"-O-rhamnosylvitexin, juglanin, afzelin, laricitrin-3-O-${\beta}$-D-glucopyranoside 및 isoquercitrin과 neolignan 화합물인 cedrusin을 단리하였다.

• Journal of the Korean Wood Science and Technology
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• 제25권2호
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• pp.81-87
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• 1997

일본잎갈나무잎을 채취하여 아세톤 : 물 (7 : 3, v/v) 의 혼합용액으로 추출한 후 에틸아세테이트용성 화합물과 수용성 화합물로 분리하였으며 Sephadex LH-20으로 충진한 칼럼을 사용하여 화합물을 단리 하였다. 단리 화합물을 확인하기 위하여 셀룰로오스 박층 크로마토그래피(TLC)를 실시한 후 자외선 램프 하에서 관찰하였다. 바닐린 발색제를 분무히여 정색반응을 조사하고 $R_r$ 값을 구하였다. 단리된 화합물들의 구조는 $^1H$-NMR과 $^{13}C$-NMR 스펙트럼을 이용하여 그 구조를 규명하였으며 에틸아세테이트용성 화합물에서는 (+)-catechin, (-)-epicatechin, kaempferol-O-arabinofuranoside와 kaempferol-3-O-arabinopyranoside, 수용성 화합물에서는 apigenin-8-C-rhamnosyl-($1"'{\rightarrow}2"$)-glucoside(2"-O-rhamnosylvitexin)을 단리 하였다.

• 약학회지
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• 제37권2호
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• pp.193-197
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• 1993

For the investigation of medicinal resources in Crataegus species, a study was carried out to clarify the constituents in the leaves of Crataegus Pinnatifida var. pubescens (Rosaceae), of which fruits have been used to stomachic, digestive, astringent, analgesic, and cardiotonic, etc. as a folk medicine in Korea. From the n-BuOH fraction of MeOH extract two flavone glycosides, vitexin(apigenin-8-C-$\beta$-D-glucopyranoside) and 2"-O-rhamnosyl vitexin(apigenin-8-C-$\alpha$-L-rhamnosyl-(1$\rightarrow$2)-$\beta$-D-glucopyrano side) were isolated and identified on the basis of their physico-chemical properties and spectroscopic evidences(UV, IR, NMR, FAB-Mass etc.) in comparision with authentic samples.amples.

• 생약학회지
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• 제36권2호통권141호
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• pp.121-128
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• 2005

In order to find the antioxidative compounds, fractionation of the MeOH extract of the leaves of Crataegus pinnatifida guided by DPPH scavenging test furnished seven phenolic compounds, $quercetin-3-O-{\alpha}-L-rhamnopyranosyl(1{\rightarrow}6)-{\beta}-D-glucopyranoside$ (1), myricetin-3-O-rhamnose (2), $quercetin-3-O-{\alpha}-L-rhamnopyranosyl-(1{\rightarrow}6)-{\beta}-D-galctopyranoside$ (3), $quercetin-3-O-{\beta}-D-galactopyranoside$ (4), quercetin (5), $apigenin-8-C-{\beta}-L-rhamnopyranosyl-(1{\rightarrow}6)-{\beta}-D-glucopyranoside$ (2'-O-rhamnosylvitexin) (6) and (-)-epicatechin (7). All of isolated compounds showed the significant antioxidative effect on DPPH free radical scavenging test and TBARS assay.

• International Journal of Advanced Culture Technology
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• 제7권3호
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• pp.120-125
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• 2019

In this paper, we have isolated six phenolic compounds, such as (+)-catechin (1), taxifolin (2), taxifolin-3-O-${\beta}$-D-(+)-xylose (3), quercetin (4), quercetin-3-O-${\alpha}$-L(+)-rhamnose (quercitrin) (5), apigenin-8-C-rhamnosyl-(1'''${\rightarrow}$2'')-glucoside (2''-O-rhamnosylvitexin) (6) from the EtOAc(Ethyl Acetate) and $H_2O$ soluble fractions of Japanese anise(Illicium anisatum L) leaves and twigs. Also, we have evaluated antioxidative and antiviral activity for each isolated compound. The antioxidative test was DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging activity. According to the experimental results, all of the isolated compounds indicated the increased radical scavenging activities as the concentration increases and most of the isolated compounds indicated generally good antioxidative values compare to the controls, ascorbic acid and ${\alpha}$-tocopherol. In the antiviral activities, all of the isolated compounds had no potentials in rhinovirus 1B (HRV 1B). But in enterovirus 71 (EV 71) and Influenza virus A/PR/8 (Influenza PR8), only quercetin (4) indicated the good antiviral activity compare to the control. Based on the above results, we found that the phenolic compounds of Japanese anise may be applied for one of the natural biomass sources that can be used as an antioxidant and an antiviral substance.