The brown planthopper, Nilaparvata lugens, is among the most serious insect pests of rice. It is widely distributed in Asia, Australia and Pacific islands. An earlier mitochondrial DNA study revealed that there exist significant genetic differences between populations north and south of the Red River Delta region in Vietnam. However the mitochondrial DNA was not sufficiently variable to examine the sources of immigration. For a more detailed analysis of geographic population structure of N. lugens, we developed microsatellite markers. Thirty-seven putative microsatellite loci were isolated using a magnetic biotin method, and five primer pairs designed from the flanking regions of sequenced microsatellite clones were labeled with fluorescent. Of these five primer sets, two have proven to be useful across all the samples we used in this study. We used variation at these two microsatellite loci to test the hypothesis that N. lugens biotypes (1, 2, and 3) sampled from laboratory selection constituted distinct genetic units. Allele frequency differences among the three major biotype categories were not significantly different at one locus (27035). However, the other (7314) did show differences among the major three biotypes. The methods we describe here will be useful for studying population structure of crop pest and for tracking the patterns of migratory pest like the rice planthoppers.
Ha, J.J.;Rhee, Y.J.;Cho, J.H.;Jang, W.J.;Kim, Y.W.;Li, Shao-Gang;Song, Y.H.
Journal of Animal Environmental Science
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v.14
no.1
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pp.9-14
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2008
This study was carried out to determine the behavioral characteristics on season and group size of Hanwoo steer. Forty eight Hanwoo steers were video-recorded at 15, 17 and 19 months of age for behavioral measurement. Space allowance was kept consistence with either group. The results showed that eating behavior of Hanwoo steers was strongly influenced (p<0.05) during seasonal transition, being the lowest in summer and the highest in autumn. With seasonal transition, standing time would linearly increase (p<0.05). In summer, steers spent more (p<0.05) time tying down but relatively less (p<0.05) time walking and fighting, meanwhile they much less (p<0.05) scratching and rubbing. In autumn, steers showed more grooming behavior (p<0.05) contrasted to the other seasons. Steers spent more (p<0.05) walking time when raised in large group and increased (p<0.05) grooming frequency when raised in small group.
Han Jung-Suk;Ko Il-Sun;Kang Kyu-Sook;Song In-Ja;Moon Seong-Mi;Kim Sun-Hee
Journal of Korean Academy of Fundamentals of Nursing
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v.6
no.3
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pp.493-506
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1999
The purpose of the study is to evaluate the effectiveness and competence level of trainees of Cardiopulmonary resuscitation training targeted for nursing students. 70 nursing students of Y nursing college are recruited as subjects from Dec. 1st, 1998 through Dec. 8th. 1998. For the pre-test. demographic data related to CPR and knowledge of CPR were evaluated. For the post-test, the next week of pre-test, three difference groups of subjects were tested their knowledge of CPR. CPR training was designed by two components which were 90 mins lecture and demonstration by one professor and individual practice using two educational models with two professors. As the tool of measurement estimating pre or post knowledge of CPR. questionnaires were developed based on self-diagnosis questionnaires of American Heart Association(AHA). The questionnaires were multiple choices (50 questions) and open end questions regarding CPR process. Each multiple choices questions valued 2 points (Score varied min. 0 point to max 100 points.). Collected data were computerized and analyzed by SPSS-WIN. Frequency and percentage of each questions analyzed. The differences of the knowledge and competency level of subjects between pre and post test was analyses by paired t-test. The followings are research outcome. 1. In the pre-test, 95% of subjects answered that they already knew what CPR was. but only 82% described correctly what CPR was. 49% learned CPR before, and 80s5 of them learned at high school. 2. 37 questions scores increases, and 10 questions scores decreased. 3 questions didnt change their score. After getting training, ratio of 80% correct score significantly increased 4 times. 3. In post-test. knowledge level of trainees increased compared to that of pre-test. (t=-15.075, p=.000) 4. Competence level also increased (t=-14.86, p=.00). In result, after getting CPR training, most CPR knowledge increased except open the air tract, toddler CPR, and alternative behavior when the air tract is blocked. CPR training needs to extend the educational scope not only CPR lecture but also psychomotor skill practice. CPR trainees are in need of appropriate feedback as well as enough opportunities of skill practice.
The Tongcheng pig breed is a famous Chinese indigenous breed. The Ministry of Agriculture of China has filed it as 1 of 19 national key conservation breeds selected from more than 100 Chinese indigenous pig breeds in 2000. In order to improve the reproductive performance, it has been intensively selected to increase the litter size for about 10 years. The population randomly sampled from conservation nucleus of eight families in the Tongcheng pigs was genotyped for identification of their estrogen receptor locus polymorphisms with the PCR-RFLPs method. Only AB heterozygotes and BB homozygotes were detected, and $X^2$ test demonstrated that the locus was in disequilibrium at a significant level (p<0.05). In the present paper, the litter sizes in different parities were regarded as different traits. Holistic status of other unspecific and unidentified genes was estimated by using the statistical methods. Coefficients of kurtosis and skewness showed that the litter size still presented segregating characteristic in the 2nd, 5th, 7th, 8th and 9th parities. Analysis of homogeneity of variance between families confirmed the results for the 5th, 7th and 8th parities. The heritability of litter size for the 1st to 10th parities was estimated with paternal half-sib model and individual estimated breeding values (EBVs) were evaluated by a single trait animal model as well. We found that the averages of EBVs for litter size in each parity did not differ significantly between genotypes, despite the significant difference for original phenotype records in the 3rd, 4th and 5th parities (p<0.05 or p<0.01). The results may be explained by the deduction that the polymorphisms of ESR locus are no longer the important genetic base of litter size variation when the frequency of allele B accumulated in the experience of selection procedure, and further conferring that there exist special genes associated with litter size in the recent Tongcheng pigs population can be made.
Journal of the Korea Academia-Industrial cooperation Society
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v.12
no.10
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pp.4493-4501
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2011
This study was performed to determine the levels of MMSE-K among the elderly people affiliated with long-term care insurance services in rural areas, and to reveal its association with related variables. The interviews were performed, during the period from March 1st, to May 31th, 2009, to 410 elderlies in rural areas. As a results, The prevalence of cognitive functions among all subjects were 80.5% of severe cognitive impairment, 11.7% of mild cognitive impairment, and 7.8% of normal. The prevalence of cognitive functions were lower according to the higher grade of long-term care insurance. And the prevalence of cognitive functions were lower in the group of lower educational level, unemployed, live alone, lower monthly income, bear for living expenses by sons and daughters and medical aid, lower visual acuity, with urinary incontinence, with amnesia, without regular exercise and lower frequency of going out than their respective counterparts. The hierarchial multiple regression analysis was used to reveal the explanatory powers of factors influencing on the cognitive functions level. Such factors as age, bear for living expenses, visual acuity, urinary incontinence, amnesia, regular exercise and grade of long-term care services were significantly influenced with cognitive functions. especially the factors of health status were significantly influenced with cognitive functions.
Recently, almost all industries are focusing on improving the competency of related industry workers, and it is necessary to design a systematic educational activity plan that constructs, operates and evaluates learning experiences for knowledge, technology and literacy development required in the industrial field. This study performed a questionnaire survey on students' satisfaction with 2015 and 2014 curriculum and national competency standards (NCS)-based one which has been applied since March 2016 against those from Department of Beauty & Cosmetology, 'C' University. For data analysis, frequency analysis, qualitative analysis and SWOT analysis were conducted, and the results found the followings: The NCS-based curriculum which has been applied since 2016 is more advanced than 2015 and 2014 curriculum in terms of strength, weakness, risk and opportunity. In addition, their satisfaction with curriculum was more than average with 47.9-57.2%. Meanwhile, when asked their favorite time to decide their academic major, 79.5% answered, '2nd semester of the 1st year'. They were very dissatisfied with the fact that their academic major is decided in the second year. It is anticipated that the NCS-based 2-year curriculum for Dept. of Beauty & Cosmetology is redesigned through analysis of students' satisfaction and by reflecting their opinions. It is also expected that the reorganization of the curriculum in a more practical and systematic manner would increase students' satisfaction and generate workforce for cosmetology industry.
The purpose of this study is to provide basic data for the development of pre-parent education program by grasping parent role recognition, educational needs and readiness of unmarried men and women. The subjects were 196 unmarried male and female students from K-do. Data were collected from November 1st to 20th, 2018, and analyzed using IBM SPSS WIN/21.0 to get frequency, percentage, average, standard deviation, t-test, ANOVA, sheffe-test, paired t-test, and IPA matrix. The average score of recognition of social pre-parent role was $3.41{\pm}0.27$ (out of 5 points), educational needs was $3.48{\pm}0.41$ points (out of 4 points), and educational readiness was $2.65{\pm}0.57$ points (out of 4 points). educational needs being higher than educational readiness. Through IPA matrix analysis, the area to be strengthened was 'family relation or parent role', and 'self understanding' was the area to encourage putting effort into other evaluation attributes. The area where no further effort is needed and needs improvement in the future was 'spouse selection and marriage', and 'pregnancy and childbirth' and 'child rearing' were the areas to intensively improve. Therefore, there is a need for establishing a standardized strategy in the area A, which has high degree of readiness and needs. Also, it is necessary to take strategies and countermeasures to raise the readiness of area C with low degree of readiness and needs, rather than accepting it as an unnecessary area of further effort input.
Journal of the Korea Academia-Industrial cooperation Society
/
v.22
no.3
/
pp.44-50
/
2021
This study is a descriptive research study to determine the effect of job stress and job satisfaction of 119 paramedics on their job performance ability. The subjects were 119 paramedics working at the fire department who agreed to participate in the study, and data collection was conducted from July 1st to September 10th, 2020. Data analysis was performed using the SPSS 23.0 program, with frequency and percentage, mean and standard deviation, correlation analysis, t-test, and ANOVA. As a result, the average job stress, job satisfaction, and job performance ability of 119 paramedics were 2.85±0.29 points, 3.35±0.30 points, and 3.53±0.89, respectively. A higher job satisfaction indicated higher job performance ability. An examination of the characteristics of the variables according to the general characteristics showed that the job performance ability was related significantly to the marital status, age, and first aid experience. Based on this, to establish a positive professional intuition to increase the job performance ability of 119 paramedics, lower job stress, and increases job satisfaction, it is important to raise awareness of job satisfaction, establish a job view, and promote job satisfaction through performance improvement. Program development and education programs for children should be actively carried out.rough performance improvement. Program development and education programs for children should be actively carried out.
Journal of Korea Entertainment Industry Association
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v.13
no.2
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pp.177-186
/
2019
The purpose of this study was to investigate the influences of the smartphone overuse on health and academic impairment in adolescents. This study used data from Korea youth risk behavior web-based survey of 2017. This survey was conducted on 64,991 adolescents(middle and high school students), and 62,276 (95.8%) responded. The researchers used frequency analysis, independent t test, chi-square test and Pearson correlation analysis using SPSS 22. As a result, the usage rate of adolescents's smartphone was 54,603 out of 62,276, which was 87.7%. The purpose of smartphone usage was messenger(1st rank, 27.3%), SNS(2nd rank, 18.7%) and game(3rd rank, 13.3%). The average daily use time of the smartphone was 206.68±194.73 minutes. Girl students showed significantly more use time of smartphones than boy students(p<.001). Students with more than 206 minutes of smartphone use had worse health and academic performance than students with less than 206 minutes(p<.001). Time of smart phone usage and academic ability showed a weak correlation(p<.001, r=.245). The present findings showed that the higher the smartphone usage, the lower the health level and academic ability, and the author discussed these results.
The Journal of the Society of Stroke on Korean Medicine
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v.9
no.1
/
pp.14-18
/
2008
Objectives : This study was done to investigate the relationship of differentiation of the patten identification in stroke patients between acute and convalescent stage. Methods : In the time of period Apr. 1st 2007 to Sep. 29th 2008, 903 patients with stroke admitted in the department of Internal Medicine of Kyungwon University Oriental Medical Hospital, Kyunghee University Oriental Medical Hospital, Dongguk University Oriental Medical Hospital were included. Patients were hospitalized within a month after the onset of stroke. Stroke patients were interviewed by doctors who studied standard operation procedures. A questionnaire was completed by a question-and-answer form between patients and doctors after explanation details to patients and the agreement of patients. Results : The frequency of fire-heat patten was the highest in acute stage of stroke and the one of deficiency of Yin group was the highest in convalescent stage of stroke. This result doesn't have statistical significance. (p<0.05) Conclusions : In this study, we found the relationship of differentiation of the patten identification in stroke patients between acute and convalescent stage. Further, we have concluded that this difference should be considered in the management for stroke patients.
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