Browse > Article

Isolation and Characterization of Microsatellites in the Brown Planthopper, Nilaparvata lugens $St{\aa}l$  

Mun Jeomhee (Division of Insect Biology, Environmental Science, Policy and Management, University of California)
Song Yoo Han (Institute of Agriculture and Life Science, Gyeong-Sang National University)
Roderick George K. (Division of Insect Biology, Environmental Science, Policy and Management, University of California)
Publication Information
Korean journal of applied entomology / v.43, no.4, 2004 , pp. 311-315 More about this Journal
Abstract
The brown planthopper, Nilaparvata lugens, is among the most serious insect pests of rice. It is widely distributed in Asia, Australia and Pacific islands. An earlier mitochondrial DNA study revealed that there exist significant genetic differences between populations north and south of the Red River Delta region in Vietnam. However the mitochondrial DNA was not sufficiently variable to examine the sources of immigration. For a more detailed analysis of geographic population structure of N. lugens, we developed microsatellite markers. Thirty-seven putative microsatellite loci were isolated using a magnetic biotin method, and five primer pairs designed from the flanking regions of sequenced microsatellite clones were labeled with fluorescent. Of these five primer sets, two have proven to be useful across all the samples we used in this study. We used variation at these two microsatellite loci to test the hypothesis that N. lugens biotypes (1, 2, and 3) sampled from laboratory selection constituted distinct genetic units. Allele frequency differences among the three major biotype categories were not significantly different at one locus (27035). However, the other (7314) did show differences among the major three biotypes. The methods we describe here will be useful for studying population structure of crop pest and for tracking the patterns of migratory pest like the rice planthoppers.
Keywords
벼멸구;마이크로새털라이트;생태형;개체군 구조;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Pathak, M.D., C.H. Cheng and M.E. Fortuno. 1969. Resistance to Nilaparvata impicticeps and N. lugens in varieties of rice. Nature 223: 502-504   DOI
2 Saxena, R.C. and A.A. Barrion. 1985. Biotypes of the brown planthopper Nilaparvata lugens (St$\aa$l) and strategies in deployment of host plant resistance. Insect Sci. Applic. 6: 271- 289
3 Vos, P., R. Hogers, M. Bleeker, M. Reijans, T. van de Lee, M. Hornes, A. Frijters, J. Pot, J. Peleman, M. Kuiper and M. Zabeau. 1995. AFLP: a new technique for DNA fingerprinting. Nucleic Acid Res. 23: 4407-4414   DOI   ScienceOn
4 Mun, J.H., Y.H. Song, K.L. Heong and G.. K. Roderick. 1999. Genetic variation among Asian populations of rice planthoppers, Nilaparvata lugens (St$\aa$l) and Sogatella furcifera Horvath (Homoptera: Delphacidae): Mitochondrial DNA sequences. Bull. of Entomol. Res. 89: 245-253
5 Song, Y.H. 1995. Risk Assessment for the monsoonic migratory pests of rice in Korea. Pages 101-112 in Proceedings of International Workshop on the Pest Management Strategies in Asian Monsoon Agroecosystems, eds. N. Hokyo and G. Norton, Kumamoto, Japan: Kyushu National Agricultural Experiment Station
6 Uhm, K.B., J.S. Park, Y.I. Lee, K.M. Choi, M.H. Lee and J.O. Lee. 1988. Relationship between some weather conditions and immigration of the brown planthopper, Nilaparvata lugens St$\aa$l. Kor. J. Appl. Eetomol. 27: 200-210
7 Kisimoto, R and K Sogawa. 1995. In Insect Migration: Tracking resources through space and time. V.A. Drake and A.G. Gatehouse eds, pp. 67-91. Cambridge, Cambridge University Press
8 Kisimoto, R. 1976. Synoptic weather conditions inducing long distance migration of planthoppers, Sogatella furcifera Horvath and Nilaparvata lugens St$\aa$l. Ecol. Entomol. 1: 95-109   DOI
9 Schneider, S., J-M. Kueffer, D. Roessli and L. Excoffier. 2001. Arlequin ver 2.1. Geneva: http://anthropologie.unige.ch/arlequin, University of Geneva
10 Wright, S. 1931. Evolution in Mendelian populations. Genetics 16: 97-159
11 Sogawa, K. 1997. Overseas immigration of rice planthoppers into Japan and associated meteorological systems. Pages 13-35 in Proceedings of China-Japan Joint Workshop on Migration and Management of Insect Pests of Rice in Monsoon Asia, China National Rice Research Institute, Hangzhou, China
12 Zane, L., L. Bargelloni and T. Patarnello. 2002. Strategies for microsatellite isolation: a review. Mol. Ecol. 11: 1-16   DOI   ScienceOn
13 Tanaka, K. 1999. Quantitative genetic analysis of biotypes of the brown planthopper N. lugens. Entomol. Exp. et Appl. 90: 279-287   DOI   ScienceOn
14 Roderick, G.K. 1996. Geographic structure of insect populations: gene flow, phylogeography, and their uses. Ann. Rev. of Entomol. 41: 263-90