• Title/Summary/Keyword: 18-03

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Fractionation and Physicochemical Characteristics of Caprine Casein by DEAE-Cellulose (DEAE-Cellulose에 의한 산양유 Casein의 분별 및 이화학적 성질에 관한 연구)

  • Im, Dong-Hyeon;Jeon, U-Min;Han, Gyeong-Sik;Kim, Byeong-Cheol;Hwang, Gwang-Yeon;Kim, Se-Heon
    • Journal of Dairy Science and Biotechnology
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    • v.18 no.1
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    • pp.1-8
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    • 2000
  • This experiment was carried out to study fractionation and physicochemical characteristics of caprine casein. Acid caseins obtained from caprine colostral and normal milk were analyzed by chymosin treatment and fractionated by DEAE-cellulose column chromatography with linear gradient and electrophoresis. Protein, fat and lactose of caprine normal milk were 2.70${\pm}$0.27%, 3.82${\pm}$0.51%, and 4.10${\pm}$0.29%, respectively. More non-protein nitrogen(NPN) was released by chymosin treatment from caprine colostral casein than normal casein. The electrophoretic pattern of caprine casein was not similar to that of bovine casein, Caprine normal casein was fractionated by DEAE-cellulose column chromatography with a 0.08${\sim}$0.18 M NaCl linear gradient into 5 pes with the proportion of 5.27%, 26.07%, 25.97%, 30.40% and 12.29%, respectively. In order to identify the pure fraction, the chymosin-treated caprine normal casein was fractionated by DEAE-cellulosecolumn chromatography with a 0.08${\sim}$0.18 M NaCl linear gradient into 6 peaks with the proportion of 17.06%, 9.10%, 17.85%, 20.11%, 27.03% and 8.85%, respectively.

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Tailoring Low-field Strain Properties of [0.97Bi1/2(Na0.78K0.22)1/2TiO3-0.03LaFeO3]-Bi1/2(Na0.82K0.18)1/2TiO3 Lead-Free Relaxor/Ferroelectric Composites (무연 완화형/정규 강유전체 복합소재 [0.97Bi1/2(Na0.78K0.22)1/2TiO3-0.03LaFeO3]-Bi1/2(Na0.82K0.18)1/2TiO3의 저전계 전계유기 변형 특성 연구)

  • Hong, Chang-Hyo;Kang, Jin-Kyu;Jo, Wook;Lee, Jae-Shin
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.29 no.6
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    • pp.342-347
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    • 2016
  • We investigated the effect of $Bi_{1/2}(Na_{0.82}K_{0.18})_{1/2}TiO_3$ (BNKT) modification on the ferroelectric and electric-field-induced strain (EFIS) properties of lead-free $0.97Bi_{1/2}(Na_{0.82}K_{0.18})_{1/2}TiO_3-0.03LaFeO_3$ (BNKTLF) ceramics as a function of BNKT content (x= 0, 0.1, 0.2, 0.3, 0.5, and 1). BNKT-modified BNKTLF powders were synthesized using a conventional solid-state reaction method. As the BNKT content x increased from 0 to 1 the normalized electric-field-induced strain ($S_{max}/E_{max}$) was observed to increase at relatively low fields, i.e., below the poling field. Moreover, BNKTLF-30BNKT showed about 460 pm/V as low as at 3 kV/mm, which is a considerably high value among the lead-free systems reported so far. Consequently, it was confirmed that ceramic-ceramic composite, a mixture of an ergodic relaxor matrix and embedded ferroelectric seeds, is a salient way to make lead-free piezoelectrics practical with enhanced EFIS at low field as well as less hysterical.

A Study on Analytical Method of Phthalate Esters in Water by SPE(Solid-Phase-Extraction) (고체상 추출법을 이용한 Phthalate Esters의 분석방법 연구)

  • 홍성희;한개희;이찬형;이순화
    • Journal of Environmental Science International
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    • v.12 no.1
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    • pp.81-86
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    • 2003
  • The study was carried out to evaluate the new analytical method of phthalate esters(diethylphthalate, di-n-butylphthalate, butylbenzylphthalate, bis(2-ethylhexyl)phthalate), one of the endocrine disruptors, which were performed by GC/MS-SIM(selected ion monitoring). The phthalate esters were extracted from water samples using solid-phase extraction on $C_{18}$ columns. It investigated that the extraction recovery rate of phthalate esters with different solvents and solvent volume. The optimal solvent was dichloromethane and proper volume of dichloromethane for recovery of phthalate esters was 4 mL. There were good linearities(above $R^2$=0.9975) in the range 0.01~0.50mg/L, and the detection limits were below 0.01~0.03$\mu\textrm{g}$/L. The recovery rates, RSD and MDLs for phthalate esters were 80~114%, 5.0~8.1% and 0.03~0.11$\mu\textrm{g}$/L, respectively. This method shows a good precision of phthalate esters.

수학 영재교육의 최근 동향에 대한 분석 - APCR 8, ERIC, ICME 10, ICCME&EGS'03을 중심으로 -

  • Lee, Gang-Seop;Hwang, Dong-Ju
    • Communications of Mathematical Education
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    • v.18 no.3 s.20
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    • pp.199-208
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    • 2004
  • 본 연구는 2004년은 한국에서 개최된 APCG 8(The 8th Asia-Pacific Conference on Giftedness, 2004)을 통하여 일반 영재교육의 최근 동향을 탐색하였다. 1980에서 2002년까지의 ERIC(Education Research Information Center)에 나타난 수학영재관련 문헌을 검색하여 644개를 선정 분석하여 그 연구 동향을 파악하고, 2003년에서 2004년까지는 ICME 10(the 10th International Congress on Mathematical Education, 2004)과 ICCME&EGS'03 (The Third International Conference Creativity in Mathematics Education and the Education of Gifted Students, 2003)을 통하여 수학영재교육의 최근 연구 동향을 분석하여 수학영재교육 발전과 활성화에 도움을 주는데 목적을 두고 있다.

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Variation of the Electrophoretic Mobility of Acid Mucopolysaccharides in Polyacrylamide Gel Electrophoresis (Polyacrylamide Gel Electrophoresis에 있어서 酸性 Mucopolysaccharide의 移動度의 變化)

  • Geum, Gi-Chang;Ru Do Wi Keu Jul, Ri O
    • Journal of the Korean Chemical Society
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    • v.18 no.2
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    • pp.132-137
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    • 1974
  • Acid mucopolysaccharides were isolated from nucleus pulposus of whale embryo. The separation of acid mucopolysaccharides was most excellent in $0.03{\%}$ hexamine cobaltic chloride in 0.05 M-sodium acetate buffer solution at pH 4.8. Changes in electrophoretic mobility of acid mucopolysaccharides were observed when the sample solution on top of spacer gel were covered with $40{\%}$ sucrose solution. This effect was not observed by the addition of hexamine cobaltic chloride to the buffer solution.

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Chemical Composition and Nutritional Value of Algae Meal Produced from Dairy Cow Wastes as a Feedstuff (젖소폐기물에서 생산된 Algae Meal의 화학적조성 및 사료적가치)

  • Kim, Y.K.;Eun, J.S.;Kim, S.D.
    • Journal of the Korea Organic Resources Recycling Association
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    • v.6 no.1
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    • pp.75-85
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    • 1998
  • Algal meal (cell) was produced from the solution of dairy cow wastes by fermentation of ulothrix. sp. and chlorella sp. Raw wastes mainly feces were diluted with ground water to give dry matter concentration of 0.5 w/v of wastes in 20 l amounts of ten plastic containers. Each containers were covered with plastic nets and vinyl films to protect from the insects and rain. Algea cells were harvested every 3 to 5 days and dried by sunlight and artifitial heat. Dried cells were ground by a feed meal, and analyzed and tested for the chemical composition of dry cell, in vitro DM and protein digestibility and the safty of algae. Protein contents in algae meals, ulothrix (29.37%) and chlorella (29.24%) were similar. However, chlorella contained lower Neutral detergent fiber (5.92%) than ulothrix(20,76%), and higher ash (32.86%) and calcium (12.62%) than ulothrix (28.66% and 6.09%) (P<.01). Ulothrix protein had higher for essential amino acids; valine, isoleucine and phenylalanine, than chlorella (P<.05). Algal fats contained high saturated fatty acids, C16:0 and C18:0, for ulothrix and high unsaturated fatty acids, C18:1 and C18:2, for chlorella (P<.01). In vitro digestibility of. ulothrix tended to be higher for DM, but lower for protein than chlorella. The weight gain and survival percentage were higher for pond fishes (loaches, Misgurnus sp. ) fed diet added chlorella meal than diets added ulothrix meal and control diet (P<.05).

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The Clinical Effects of Normocapnia and Hypercapnia on Cerebral Oxygen Metabolism in Cardiopulmonary Bypass (체외순환 시 뇌대사에 대한 정상 탄산분압과 고 탄산분압의 임상적 영향에 관한 비교연구)

  • 김성룡;최석철;최국렬;박상섭;최강주;윤영철;전희재;이양행;황윤호
    • Journal of Chest Surgery
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    • v.35 no.10
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    • pp.712-723
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    • 2002
  • Substantial alterations in cerebral blood flow(CBF) are known to occur during cardiopulmonary bypass(CPB). Many investigators have speculated that these changes may be responsible for both minor and major cerebral damages after CPB. More recently, these changes in CBF have been observed to be intimately related to the arterial carbon dioxide tension(Pa$CO_2$) maintained during CPB. The present study was prospectively designed to investigate the clinical effects of normocapnic and hypercapnic CPB on the cerebral oxygen metabolism in cardiac surgery Material and Method: Thirty-six adult patients scheduled for elective cardiac surgery were randomized to either normocapnic group (Pa$CO_2$35~40 mmHg, n=18) or hypercapnic group(Pa$CO_2$, 45~55 mmHg, n=18) with moderately hypothermic nonpulsatile CPB(nasopharyngeal temperature of 29~3$0^{\circ}C$). In each patient, middle cerebral artery blood flow velocity( $V_{MCA}$), cerebral arteriovenous oxygen content difference (C(a-v) $O_2$), cerebral oxygen extraction(COE), cerebral metabolic rate for oxygen(CMR $O_2$), cerebral oxygen transport( $T_{E}$ $O_2$), $T_{E}$ $O_2$/CMR $O_2$ ratio, cerebral desaturation(internal jugular bulb blood oxygen saturation $\leq$ 50%), and arterial and jugular bulb blood gas were evaluated throughout the operation. Postoperative neuropsychologic complications were assessed in all patients. All variables were compared between the two groups. Result: VMCA(169.13 $\pm$ 8.32 vs 153.11 $\pm$8.98%), TE $O_2$(1,911.17$\pm$250.14 vs 1,757.40$\pm$249.56), $T_{E}$ $O_2$,/CMR $O_2$ ratio(287.38$\pm$28.051 vs 246.77$\pm$25.84), $O_2$ tension in internal jugular bulb (41.66$\pm$9.19 vs 31.50$\pm$6.09 mmHg), and $O_2$saturation in internal jugular bulb(68.97$\pm$10.96 vs 58.12$\pm$12.11%) during CPB were significantly lower in normocapnic group(p=0.03), whereas hypercapnic group had lower C(a-v) $O_2$(3.9$\pm$0.3 vs 4.9$\pm$0.3 mL/dL), COE(0.3$\pm$0.03 vs 0.4$\pm$0.03), CMR $O_2$(5.8 $\pm$0.5 vs 6.8$\pm$0.6), and arterial blood pH(7.36$\pm$0.09 vs 7.46$\pm$0.07, p=0.04) during CPB. Hypercapnic group had lower incidence of cerebral desaturation than normocapnic group(3 vs 9 patients, p=0.03). Duration of the neuropsychologic complication(delirium) were shorter in hypercapnic group than in normocapnic group(36 vs 60 hrs, p=0.009). Conclusion: These findings suggest that hypercapnic CPB may have salutary effects on the cerebral oxygen metabolism and postoperative neurologic outcomes in cardiac surgery.surgery.

Thermoluminescence from X-Ray Irradiated Beta-Eucryptite (X-선 조사된 Beta-eucryptite의 열자극 발광)

  • 김태규;이병용;최범식;강현식;추성실;황정남
    • Progress in Medical Physics
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    • v.3 no.1
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    • pp.9-18
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    • 1992
  • In this study, beta-eucryptite is fabricated and the thermal parameters of this material have been investigated. The thermoluminescence from 4MeV X-ray irradiated beta-eucryptite have been measured over the temperature range of 300K-600K. Thermoluminescence curve from X-ray irradiated beta-eucryptite shows five peaks located at 342K, 392K, 438K, 474K, and 527K. $\tau$, $\delta$ and $\omega$ of peak at 527K are 35K, 39K and 74K, respectively and this peak is found to be 2nd order kinetics. The activation energy of peak shape method is calculated to be 1.03eV and the frequency factor for 527K curve is calculated to be 3.9x10$\^$8/sec$\^$-1/. Based on the various heating rates methods, the activation energy of the peak is computed to be 1.02${\pm}$0.05eV that is similar to 1.19${\pm}$0.03eV of initial rise method. The linearity of thermoluminescence intensity and radiation flux is valid up to 50Gy and beyond higher dose the supralinearity and saturation come out.

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Analgesic Effects of Taxus cuspidata Pharmacopuncture Using Different Extraction Methods on the Formalin-Induced Pain Model (추출 방식의 차이에 따른 주목약침이 Formalin 유도 통증 모델의 진통에 미치는 영향)

  • Shin, You Bin;Lee, Hyun Jong;Kim, Jae Soo;Lee, Yun Kyu
    • Journal of Acupuncture Research
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    • v.33 no.3
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    • pp.17-28
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    • 2016
  • Objectives : This study was designed to investigate the analgesic effects of Taxus cuspidata pharmacopuncture(TCP) at KI03 on formalin-induced pain in rats and to evaluate the efficiency of different extraction methods of Taxus cuspidata(TC). Methods : 30 rats were divided into 5 groups, each consisting of 6 rats. Each of the groups was treated two times. The first treatment for its right KI03 was as follows: rats were treated with normal saline(NOR), normal saline(CON), hot-water extraction of TC(W), ethanol extraction of TC(E), and ultrasonification extraction of TC(U). The second treatment was given 35 mins after the first one. Rats in NOR were treated with normal saline at their hind-paw. All groups, except NOR, were treated with formalin(5 %, $40{\mu}{\ell}$) at their hind-paw. To evaluate pain behavior, Ultrasonic vocalization(USV) was examined to be around 18~30 kHz. After analysis of USV, blood samples were taken from the rats for analysis of Substance P, aspartate aminotransferase(AST) and alanine aminotransferase(ALT). Results : In the experimental groups, USV was significantly decreased compared with CON and similar to that of NOR in the early phase. But there were no significant differences among the three extraction methods of TC. In addition, Substance P of W was significantly decreased. AST and ALT activation showed no significant differences among the 5 groups. Conclusion : These results show that regardless of extraction methods of TC, TCP at KI03 has analgesic effects in the early phase on formalin-induced pain in rats. In particular, hot-water extraction of TC significantly reduces Substance P activation.

Study on Relationship between the Obesity Index and the Refractive Power in Adolescent (청소년기 비만도 지수와 굴절력과의 상관성 연구)

  • Lee, Wan-Seok;Ye, Ki-Hun;Shin, Bum-Joo
    • Journal of Korean Ophthalmic Optics Society
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    • v.18 no.3
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    • pp.329-339
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    • 2013
  • Purpose: In this study, nutrient intake during the adolescent period is a critical time for the development of the vision. Therefore we analyzed the relationship between the obesity index and the refractive power. Methods: We used the Korean National Health and Nutrition Examination Survey 2010 document. The obesity index is classified as Broca index (less than 80.0% is low weight, 80 to 89.9% is underweight, 90.0 to 109.9% is normal, overweight is from 110.0 to 119.9%, 120.0% or more is obesity) and body mass index (BMI)(less than 18.5 is underweight, 18.6~22.9 is normal, 23.0 to 24.9 is overweight, and obesity is higher than 25.0). We analyzed correlation with the body mass index and refractive error in adolescent. Results: As shown in the statistics, according to Broca index and body mass index (BMI), the refractive power and the obesity index showed a statistically significant correlation in the ages 15 to 18. Under weight subjects are ingested 43.84% dietary fiber, vitamin C 56.55%, 35.20% iron, 31.84% of, and 38.03% potassium less than the average food intake by the standard group. Conclusions: Moderate weight with a broad variety of taking nutrition and good eating habits seems to have an effect to the good growth and the good vision.