• Journal of Korean Home Economics Education Association
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• v.7 no.1
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• pp.47-55
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• 1995

The purpose of this study were to identify the importance of father’s roles on children. A survey was carried out on 458(227 boys and 231 girls) 6th-graders at randomly chosen 4 elementary schools in Kwangju. Frequency, percentage, average, standard deviation and Pearson’s coefficient were checked and one-way ANOVA, Duncan’s multiple range test and multiple regression analysis were also carried out on the collected data. The results were as follows; 1. Evaluation of fathers’role performance depending on background variables ranged from 46.17 to 58.51 points(equivalent to 61.56∼78.01 points in the 100-point scale). Background variables that caused statistically significant difference were children’s sex, fathers’religion (whether they had one or not) fathers’education, monthly family income and decision-makers at home. The degrees of fathers’role performance were higher when the answerers were girls, when fathers had religions, when fathers educational level was higher, when the family income was higher, and when all family members participated in the decision-making. 2. Evaluation of children’s achievement motivation depending on background variables ranged from 77.17 to 84.34 points( equivalent to 68.90∼75.35 points in the 100-point scale). Background variables that caused statistically significant differences were children∼s sex and decision-makers at home. Children∼s achievement motivation were higher in the case of girls and when all family members participated in the decision-making. 3. When it comes to the children’s achievement motivation in relation to father’s role performance, 1)The overall aspect of fathers’ role performance caused significant differences in children’s achievement motivation. 2) The influence of fathers’role performance on children’s achievement motivation was highest in the investigation-faciliator role (${\beta}$=2.5) and then the economic role (${\beta}$=.14) and the valuetransmitter role (${\beta}$=.14). The explanative power of these variables was 22%.

• Applied Biological Chemistry
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• v.47 no.3
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• pp.357-360
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• 2004

The flower of Campsis granudiflora K. Schum. was extracted with 80% aqueous MeOH, and the concentrated extract was partitioned with EtOAc, n-BuOH and $H_2O$. From the EtOAc fraction, four compounds were isolated through the repeated silica gel and ODS column chromatographies. From the result of physico-chemical data including NMR, MS and IR, the chemical structures of the compounds were determined as linolenic acid methyl ester, linoleic acid methyl ester, ${\beta}-sitosterol$ and daucosterol. Daucosterol inhibited FPTase activity with $IC_{50}$ values of $14{\pm}0.04\;{\mu}M$.

• Journal of Biomedical and Translational Research
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• v.19 no.4
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• pp.65-72
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• 2018

The purpose of this study was to investigate probiotic characteristics and immune enhancing effects of Bifidobacterium (B.) longum KBB1-26 and BIF-4, B. breve KBB5-22 isolated from human intestine for probiotic use in humans and animals. We measured acid, bile and heat tolerance, antimicrobial activity against pathogenic bacteria, Escherichia (E.) coli, Salmonella (S.) Enteritidis, Staphylococcus (S.) aureus, and Listeria (L.) monocytogenes. Immune enhancing effects of B. longum and B. breve were investigated by measuring nitric oxide (NO), nuclear factor ($NF-{\kappa}b$), $interleukin-1{\beta}$ ($IL-1{\beta}$), interleukin-6 (IL-6), interleukin-12 (IL-12) and tumor necrosis $factor-{\alpha}$ ($TNF-{\alpha}$) in RAW 264.7 cells or RAW BLUE cells. B. longum KBB1-26 was survived at pH 2.0. B. longum KBB1-26 and BIF-4, B. breve KBB5-22 also showed tolerance to 0.3% of oxgall bile salt. B. longum KBB1-26 was able to survive at $70^{\circ}C$ and $80^{\circ}C$ for 20 min. KBB1-26 showed the antimicrobial inhibition zone to pathogenic bacteria such as E. coli (12 mm), S. Enteritidis (14 mm), S. aureus (14 mm) and L. monocytogenes (41 mm). The production of NO ($4.5{\pm}0.00{\mu}M/mL$) and $IL-1{\beta}$ ($39.7{\pm}0.55pg/mL$) of KBB1-26 significantly higher than BIF-4 and KBB5-22, respectively. In addition, KBB1-26 and KBB5-22 induce the production of high level of $TNF-{\alpha}$ and IL-6 in macrophages. Collectively, B. longum KBB1-26 have acid, bile, heat tolerance, antimicrobial activity and immune enhancing effects. These results suggest that KBB1-26 can be used as probiotics for humans and animals.

• Journal of the Korean Applied Science and Technology
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• v.5 no.2
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• pp.9-15
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• 1988

In order to investigate the amino acid and fatty acid content in Thuja biotae water extract treated with alkaline, it was performed. There are 16 kinds of different amino acid and 20 kinds of different fatty acid in Thuja biotae water extract. An aspartic acid was contained 52％ and proline was contained 10％, particulary, r-aminobutyric acid was analysed. Essential fatty acids; linoleic acid, linolenic acid and arachidonic acid were cotained a lot amount. There are 11 different unknown materials which were identified by GC-MS spectrum, such as N-[($4{\alpha},5{\alpha}$)-cholestan-4-yl]-acetamide; 22,26-Epithio-furost-5-en-3-ol; 2-Methyl-6-(4-methyl-3-cyclohexen-1-yl)-4-heptanone; 3,12,14-Tris(acetyloxy)-pregnane-15,20-dione;22-Methyl-26-thio-furost-5-en-3-ol; 7-Ethenyl-1,2,3,4,4a,4b,5,6,7,9,10,10a-dodecahydro-1,4a,7-trimethyl-1-phenanthrene carboxyaldehyde; Methoxyiminopro-panedioic acid; 13-Methyl-13-${\beta}$-Methyl-13-vinyl-dodecaarp-7-en-3-3-ol; 22-Methyl-26-thio-furost-6-methyl-3-ol; $5{\alpha}-Androstane-2$,11-dione; 9-Methyl-heptadecanoic acid.

• Journal of Microbiology and Biotechnology
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• v.33 no.7
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• pp.926-933
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• 2023

Aspergillus oryzae KCCM 11372 was used to enhance the production of β-glucan using humidity control strategies. Under conditions of 60% humidity, solid-state fermentation (SSF) increased the yields of enzymes (amylase and protease), fungal biomass (ergosterol), and β-glucan. The maximum concentrations obtained were 14800.58 U/g at 72 h, 1068.14 U/g at 120 h, 1.42 mg/g at 72 h, and 12.0% (w/w) at 72 h, respectively. Moreover, the β-glucan containing fermented brown rice (β-glucan-FBR) extracts at concentrations of 25-300 ㎍/ml was considered noncytotoxic to 3T3-L1 preadipocytes. We then studied the inhibitory effects of the extracts on fat droplet formation in 3T3-L1 cells. As a result, 300 ㎍/ml of β-glucan-FBR extracts showed a high inhibition of 38.88% in lipid accumulation. Further, these extracts inhibited adipogenesis in the 3T3-L1 adipocytes by decreasing the expression of C/EBPα, PPARγ, aP2, and GLUT4 genes.

• Journal of Aquaculture
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• v.4 no.2
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• pp.97-110
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• 1991

This study was undertaken to understand the seasonal changes of hormone levels such as luteinizing hormone (LH), follicle stimulating hormone (FSH) and estradiol-$17{\beta}$, serum components such as albumin, total protein and triglycerides, and GSI of rainbow trout (Onco-rhynchus mykiss) under the normal-light circumstances. LH levels were the highest in November whereas FSH levels were decreased progressively from October to February. Serum GTH, such as LH and FSH, levels were low immediately after egg-stripping and were significantly increased in the summer in association with the initiation of vitellogenesis. Serum albumin, total protein, and triglycerides were significantly increased in August in association with initiation of vitellogenesis and were peak in November prior to egg-stripping. Oocyte growth, measured by oocyte diameter and GSI, was significantly increased in August and showed the highest value in January. Correlation coefficients of serum LH with FSH and estradiol-$17{\beta}$ were +0.844 and +0.947, respectively. Correlation coefficients of estradiol-$17{\beta}$ with albumin and total protein were +0.634 and +0.859, respectively. Correlation coefficients of serum estradiol-$17{\beta}$ with triglycerides and GSI were +0.673 and +0.694, respectively. Probably by positive feedback mechanism, LH, FSH and estradiol-$17{\beta}$ appeared to stimulate to liver to secrete albumin, total protein and triglycerides for vitellosenesis.

• Journal of Environmental Health Sciences
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• v.22 no.1
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• pp.65-81
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• 1996

This study was conducted from march 20 through April 20, 1992, jn order to figure out the factors affecting behavior of the people on environmental health. 1, 261 people were selected by sampling from Seoul, Pusan, Taegu, Taejon and Kwangju. Questionnaire forms were prepared and the persons selected were-interviewed by trained interviewers. The data collected were analyzed in order to determine factors affecting kowledge, attitude and practice on environmental health in major cities in Korea. The major results are as follows: 1. The people who got high KAP score were found not to practice what they know. The people who got lower scores were found to use their knowledge in practical life. 2. Correlation between knowledge, attitude, and practice (KAP) on environmental health was statistically significant (p < 0.001). 3. The levels of KAP on environmental health affected by variables of gender and marital status were statistically significant (p < 0.01). 4. Variables of education, occupation, religion and economic status affected the levels of KAP significantly (p < 0.01). 5. The levels of KAP on environmental health by variables of health education on environmental health were statistically significant (p < 0.001). 6. The levels of KAP of the people on environmental health by demographic variables showed a reverse relation statistically (p < 0.001). 7. The levels of KAP of the people on environmental health had correlation with education level and the KAP level had correlation with knowledge (p < 0.001). 8. The KAP levels of the people on environmental health had correlation with environmental health education (p < 0.001). 9. The total variables affecting KAP of the people on environmental health had 14% variance and environmental health education was the highest($\beta$=0.23827), education level was the next($\beta$=0.12442), and economic status was ($\beta$=-0.06970), age ($\beta$=-0.06710) and print media ($\beta$=0.06539). 10. The variables most affecting KAP of the people on environmental health were environmental health education(r=0.2980) and education(r=0.2419) and the next were age, marital status, religion, electronic media, print media, place of birth and gender. 11. The most important variable affecting KAP level of the people on environmental health was education level.

• Korean Journal of Rural Living Science
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• v.5 no.2
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• pp.185-197
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• 1994

This study is to investigate the factors which affect self- esteem of the aged parents. In this study questionaires were used to measure solidarity between aged parents and adults children, self- esteem of aged parents. Subjects were 234 male and 235 female parents who were aged over 60. The data were analyzed by using frequency, percentage, mean, standard deviation, ANOVA, and stepwise regression analysis. The results are as follows : 1) The solidarity between the aged parents and their adult children is average 65.5(Conversion value by 100 : 51.9) and shows the average level. The relations between solidarity and background variables are variables affected by education, pocket money, health, social activity and religion. 2) The degree of self- esteem in the aged parents is presented to average 26.1(Conversion value by 100 : 56.5) and shows the average level and it according to the background variables has a significant difference depending on education, pocket money, family living together, health, social activity, religion and the number of children. 3) To investigate the degree of self- esteem according to the degree of solidarity, the sub-areas of solidarity are subdivided into five groups. higher solodarity the group has, higher self- esteem it has. 4) The degree of effects of the variables which affect self- esteem in the aged parents is in such order as affectional solidarity($\beta$＝0.32), helping($\beta$＝0.14), education($\beta$＝0.10), solidarity by contact($\beta$＝0.09) and solidarity by argument($\beta$＝0.09) and $R^2$ of such variables are 30%.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1995.04a
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• pp.86-86
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• 1995

The analysis of membrane receptors for hormones and neurotransmitters has progressed considerably by pharmacological and biochemical means and more recently by the use of specific anti-receptor antibodies. A 14-mer peptide (from Phe102 to Leu115 of ${\beta}$2-adrenergic receptor) was synthesized and this peptide was coupled to carrier protein Keyhole Limpet Hemocyanin(KLH) by glutaraldehyde method. A 0.5mg of KLH-coupled peptide was emulsified with equal volume of complete Freund's adjuvant and injected via popliteal lymph node to each of the three Newzealnd White rabbits. Booster injections were repeated at 4 weeks interval for three times with incomplete Freund's adjuvants. One week after the final injection, serum was prepared from ear artery. Nonspecific immunoglobulins were removed by passing the serum through KLH-Sepharose 6B affinity matrix and further by incubation with bovine lung aceton powder. The titer of the antibody for synthetic peptide which was determined by enzyme linked immunosorbent assay(ELISA) was about l/l,000. The antibody produced in this study revealed 67kDa protein band in the western blot of partially purified guinea pig lung ${\beta}$-adrenergic receptor preparation. The antibody inhibited ${\beta}$-adrenergic antaginist [3H] Dihydroalprenolol binding to soluble ${\beta}$-adrenergic receptor by 25% while control sera did not show any inhibitory effects, The result of this study suggests that the peptide sequence selected in this study may play some important roles in adrenergic receptor-ligand interaction.

• Archives of Pharmacal Research
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• v.20 no.3
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• pp.247-252
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• 1997

Perfluorotolyl (PFT)-ether and perfluorotoly-trimethylsilyl (PFT-TMS) ether derivatives of oestrone, $17{\alpha}$- and $17{\beta}$oestradiol were prepared under phase transfer conditons. The former derivatives under negative ion chemical ionization conditions gave significant ions in the mass spectrometer but $17{\alpha}$- and $17{\beta}$ -oestradiol gave poor resolution. However, the PFT-TMS derivatives of 17.${\alpha}$- and$17{\beta}$-oestradiol showed good resolution. These derivatives were used for the analysis of oestrogens in bovine aqueous humour, vitreous humour and retina. The mean $({\pm}SEM)$ concentrations of oestrone in bovine aqueous humour (n=18), vitreous humour (n=18) and bovine retina (n=4) were $0.47{\pm}0.11$, $0.46{\pm}0.14$ and $1.10{\pm}0.24 ng.ml^{-1}$, respectively. $17{\alpha}$-Oestradiol was detected in 16 out of 18 samples of bovine aqueous humour and vitreous humour and the mean $({\pm}SEM)$ concentrations were $0.30{\pm}0.10$ and $0.08{\pm}0.02 ng.ml^{-1}$, respectively. The mean $({\pm}SEM)$ concentration of 17.betha.-oestradiol in aqueous humour (n=7) and vitreous homour (n=11) $0.83{\pm}0.26 ng ml^{-1}$ and $0.39{\pm}0.09 ng ml^-1$, respectively. In retina the concentrations of both steroids were below the detection limit.