• Title/Summary/Keyword: 11S

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11S and 7S Globulin Fractions in Soybean Seed and Soycurd Characteristics (콩 종실 단백질 분획(7S, 11S)과 두부특성)

  • Kim, Yong-Ho;Kim, Seok-Dong;Hong, Eun-Hi
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.39 no.4
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    • pp.348-352
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    • 1994
  • Soybean seed consists of two major storage protein, the 7S and 11S globulins. For improving the quality of soybean seed protein, an increase of 11S/7S ratio would be a desirable objective because the 11S globulin contains much more the sulfur-containing amino acids than the 7S globulin. In this study, some soybean varieties were used to investigate the analyzing method for 7S and 11S globulins. 7S and 11S globulins couble be fractionated by their different solubilities in tris buffers. Adjusting the pH and tris concentration were major factors affecting the precipitation of the two globulins. And it was possible to screen the soybean genotypes having aberrant subunit compositions of the two globulins by an sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of total soybean proteins. The ratio of 11S to 7S globulin ranged from 1.29 to 1.38. This paper also dealed with the contribution of protein components in soybean seeds to the physical properties of soycurd. It indicated that the soycurd from crude 11S was remarkably harder than that from crude 7S, and springiness and cohesiveness were slightly higher in soycurd having higher proportion of 11S. So, it may concluded that proportion of protein components in soybean seed can be important factor which controls the suitability for soycurd or other foods.

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Genetic variation of 7S and 11S globulins in soybean seed (콩 종실 단백질의 유전변이)

    • Korean Journal of Plant Resources
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    • v.12 no.3
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    • pp.198-203
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    • 1999
  • 7S and 11S globulins are two major storage proteins in soybean seed. For improving the quality of soybean seed protein, an increase of 11S/7S ratio would be a desirable objective because 11S globulin contains much more sulfur-containing amino acids than 7S globulin. In this study, six soybean varieties grown at three locations were used for genetic variation analysis of 7S and 11S globulins. It was possible to screen the soybean genotypes having aberrant subunit compositions of the two globulins by a sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). So, heritabilities, genotypic and phenotypic correlations among eight globulin fraction contents of soybean seeds were estimated. The mean value of 7S and 11S globulin fraction contents were 38.9% and 61.1%, respectively, and the ratio of 7S to 11S globulin ranged from 0.58 to 0.74. The high heritability value was found in $\beta$ subunits but the values of acidic and basic subunits were relatively low. Genotypic correlations were higher than the corresponding phenotypic correlations in most of globulin subunit contents. $\beta$ subunits was negatively correlated with $\alpha$ and $\alpha$' subunits among 7S fractions, while no significant correlation between $\alpha$ and $\alpha$' subunits could be found In case of 11S fractions, acidic and basic subunits exhibited no genotypic but negative phenotypic correlation.

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Induction of Steroid 11 $\beta$-hydroxylase in Pellicularia fillamentosa (Pellicularia fillamentosa로부터 Steroid 11$\beta$-hydroxylase의 유도)

  • 김말남;김영숙
    • Korean Journal of Microbiology
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    • v.27 no.4
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    • pp.366-372
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    • 1989
  • Twenty-one strains were tested for 11$\beta$-hydroxylation of Reichstein's substance S. Four fungi exhibited ability for the reaction, among which Pellicularia fillamentosa showed the highest activity. The 11$\beta$-hydroxylase of this fungus was proved to be induced by the substrate, cycloheximide reducing significantly the activity of the enzyme. Range of optimum pH for the 11$\beta$-hydroxylation was broad and found to be 2.0-8.0. Test of the enzyme activity at different growing stages, from spore to mycelia, showed that the branching stage of hyphae and the mature mycelial stage were the most effective for the Reichstein's substance S transformation. However, 11$\beta$-hydroxylase in the intact spore was turned out to be uninducible with the substrate.

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Synthesis and Phytotoxic Activities of (8S, 9S, 11R)-(-)-Monocerin and (9S, 11R)-(+)-Fusarentin 4, 5-dimethyl ether ((8S, 9S, 11R)-(-)-Monocerin and (9S, 11R)-(+)-Fusarentin 4, 5-dimethyl ether의 합성과 생리활성)

  • Ko, Byoung-Seob
    • Applied Biological Chemistry
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    • v.37 no.5
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    • pp.402-408
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    • 1994
  • For the examination of the role of monocerin(1) on the biological activity, (8S, 9S, 11R)-(-)-monocerin(20) and (9S, 11R)-(+)-fusarentin 4, 5-dimethyl ether(19) were synthesized by a condensation of the benzylic anion of ethyl 2, 3, 4-trimethoxy-6-methylbenzoate(16) with modifyed (R)-ethyl 3-hydroxyhexanoate (9). In a key step, bioreduction with active dried baker's yeast in organic solvent system was employed to get a chiral aldehyde 12. Their phytotoxic activities were tested on rice seedlings and lettuce seeds.

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Overexpression and Selective Anticancer Efficacy of ENO3 in STK11 Mutant Lung Cancers

  • Park, Choa;Lee, Yejin;Je, Soyeon;Chang, Shengzhi;Kim, Nayoung;Jeong, Euna;Yoon, Sukjoon
    • Molecules and Cells
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    • v.42 no.11
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    • pp.804-809
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    • 2019
  • Oncogenic gain-of-function mutations are clinical biomarkers for most targeted therapies, as well as represent direct targets for drug treatment. Although loss-of-function mutations involving the tumor suppressor gene, STK11 (LKB1) are important in lung cancer progression, STK11 is not the direct target for anticancer agents. We attempted to identify cancer transcriptome signatures associated with STK11 loss-of-function mutations. Several new sensitive and specific gene expression markers (ENO3, TTC39C, LGALS3, and MAML2) were identified using two orthogonal measures, i.e., fold change and odds ratio analyses of transcriptome data from cell lines and tissue samples. Among the markers identified, the ENO3 gene over-expression was found to be the direct consequence of STK11 loss-of-function. Furthermore, the knockdown of ENO3 expression exhibited selective anticancer effect in STK11 mutant cells compared with STK11 wild type (or recovered) cells. These findings suggest that ENO3-based targeted therapy might be promising for patients with lung cancer harboring STK11 mutations.

A Study on Backoff Algorithm for QoS in IEEE 802.11 Wireless LAN (IEEE 802.11 Wireless LAN에서의 QoS 지원을 위한 Backoff Algorithm에 관한 연구)

  • 조재용;김석호;류동관;곽경섭
    • Journal of the Institute of Electronics Engineers of Korea TC
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    • v.40 no.12
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    • pp.116-124
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    • 2003
  • According to the convenience and efficiency, IEEE 802.11 WLAN(Wireless LAN) has became rapidly wide-spread on the market. However, QoS support is needed for various multimedia service. In this paper, we consider both MAC method of IEEE 802.11 and IEEE 802.11e and Traffic Category differentiated service by QoS support method. In addition, we study currently existing back-off algorithms in view of comparative analysis. As a result we propose a new back-off algorithm called Collision Rate Based-EDCF. The proposed back-off algorithm confirms the performance by computer simulations in terms of Throughput, and QoS support.

QoS Support to Design for IEEE802.11e based in Wireless Mesh Networks (IEEE802.11e를 기반으로 한 Wireless Mesh Networks에서 QoS 향상 방법 설계)

  • Seo, Hyung-Yoon;Kim, Dong-Hyun;Kim, Jong-Deok
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2010.05a
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    • pp.702-704
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    • 2010
  • Wireless LAN(Local Area Network) environment to support QoS in IEEE802.11 Working Group has standardized on the IEEE 802.11e. IEEE 802.11e EDCA channel access of the high priority traffic as by raising the probability of a chance to support QoS. This is a high priority traffic than low priority traffic, channel access is not necessarily the first. Therefore, as all traffic channels, if you have the same routing path, lower priority traffic on the performance of high priority traffic, sustainable impact. As a result, EDCA, such as voice or video to guarantee QoS for multimedia applications, follow the crowd. In this paper, to solve these problems by using Multi-Interface Multi-Path Routing Algorithm is proposed. Interface for each use in IEEE 802.11e EDCA Access Category much differently by each of the Routing Path for high priority traffic and guarantee QoS.

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An Analysis of Bias-Dependent S11-Parameter in Multi-Finger MOSFETs (Multi-Finger MOSFET의 바이어스 종속 S11-파라미터 분석)

  • Ahn, Jahyun;Lee, Seonghearn
    • Journal of the Institute of Electronics and Information Engineers
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    • v.53 no.12
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    • pp.15-19
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    • 2016
  • The gate bias dependence of kink phenomenon with a large deviation from the resistance circle in Smith chart is observed in the frequency response of $S_{11}$-parameter for large multi-finger RF MOSFETs. For the first time, this bias dependence is analyzed by measuring magnitude and phase of $S_{11}$-parameter, input resistance and input capacitance. As a result, $V_{gs}$ dependent $S_{11}$-parameter is largely changed by the magnitude of input capacitance as well as dominant pole and zero frequencies of input resistance. At $V_{gs}=0V$, the kink phenomenon occurs in the high frequency region because of very small phase difference of $S_{11}$-parameter and high pole frequency of input resistance. However, the kink phenomenon at higher $V_{gs}$ is generated in the low frequency region owing to large phase difference and low pole frequency.

Assessment of Isokinetic Muscle Function, Balance and Flexibility in Elite High School Kayakers (고등학교 엘리트 카약 선수들의 등속성 근기능·균형성·유연성 측정 및 평가)

  • Shin, Kwang-Taek;Sohn, Jee-Hoon
    • Journal of Digital Convergence
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    • v.16 no.6
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    • pp.407-416
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    • 2018
  • The purpose of this study is to evaluate elite high school kayaker's iso-kinetic muscle function and provide physical training advices. Biodex system IV, Biodex stabilizer system, Inbody 3.0 and CNP-5403 are used to investigate 11 kayaker's muscle function(peak torque, average power and work fatigue), dynamic balance, body compositions, and flexibility. S4, S5, S8, S10, S11 are needed to train shoulder muscle strength and power. S5, S6, S10, S11 are needed to strengthen shoulder muscle endurance. S5, S7, S8, S10, S11 are needed to train knee muscle strength and power. S1, S4, S5, S10 are needed to strengthen knee muscle endurance. S3, S10, S11 are needed to train wrist muscle endurance. Based on these results we should establish an muscle function enhancing program with periodic inspection for elite kayakers. And standardized training program should be sublated.

Changes of Protein During Growth of Soybean Sprout (Gel filtration에 의한 콩나물 제조중(製造中) 단백질(蛋白質)의 변화조사(變化調査))

  • Yang, Cha-Bum;Park, Sang-Ki;Yoon, Suk-Kwon
    • Korean Journal of Food Science and Technology
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    • v.16 no.4
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    • pp.472-474
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    • 1984
  • Gel filtration of water soluble protein of soybean and cotyledon of sprout at various growth stages by using Sephadex G-200 showed 5 fractions (A,B,C,D and E). According to gel filteration and disc gel electrophoresis, fraction B,C and D were identified as 11S,7S and 2S, respectively. Fraction A was turbid substance and fraction E showed 1 peak. During growth of sprout 7S decreased firstly, 2S secondly and 11S lastly in cotyledon. Fraction A increased until 6th day and decreased thereafter while E increased throughout the growth. In axis only two fractions (11S+7S and E) were showed, and 11S+7S fraction was little changed and fraction E increased slightly with the growth.

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