Abstract
Twenty-one strains were tested for 11$\beta$-hydroxylation of Reichstein's substance S. Four fungi exhibited ability for the reaction, among which Pellicularia fillamentosa showed the highest activity. The 11$\beta$-hydroxylase of this fungus was proved to be induced by the substrate, cycloheximide reducing significantly the activity of the enzyme. Range of optimum pH for the 11$\beta$-hydroxylation was broad and found to be 2.0-8.0. Test of the enzyme activity at different growing stages, from spore to mycelia, showed that the branching stage of hyphae and the mature mycelial stage were the most effective for the Reichstein's substance S transformation. However, 11$\beta$-hydroxylase in the intact spore was turned out to be uninducible with the substrate.
Reichstein's substance S의 $11\beta$-hydroxylation 활성이 있는 균주를 선택하고자 21 균주를 실험한 결과 4균주에서 $11\beta$-hydroxylation의 활성이 발견되었으며, 그 중 Pellicularia fillamentosark 가장 높은 효소활성을 보였다. 이 균주의 $11\beta$-hydroxylation의 합성을 강력하게 저해하였다. $11\beta$-hydroxylation의 최적 pH 범위는 2.0-8.0으로 광범위하였다. 성장단계 중 균사체 분지시기와 완전하게 성숙한 균사체 시기에 효소합성의 유도가 가장 활발하였으며, 70시간 배양된 균사체가 가장 좋았다. 반면, 포자에는 기질에 의한 $11\beta$-hydroxylation의 유도현상이 없는 것으로 판정되었다.