• Journal of Life Science
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• v.27 no.10
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• pp.1104-1110
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• 2017

SET1A is a histone H3K4 methyltransferase that catalyzes di- and trimethylation of histone H3 at lysine 4 (H3K4). Mono-, di-, and trimethylations on H3K4 (H3K4me1, H3K4me2, and H3K4me3, respectively) are generally correlated with gene activation. Although H3K4 methylation is associated with the stimulation of adipogenesis of 3T3-L1 preadipocytes, it remains unknown whether SET1A plays a role in the regulation of adipogenesis of 3T3-L1 preadipocytes. Here, we investigated whether SET1A regulates 3T3-L1 preadipocytes' adipogenesis and characterized the mechanism involved in this regulation. SET1A expression increased during 3T3-L1 preadipocytes' adipogenesis. Consistent with the increased SET1A expression, the global H3K4me3 level had also increased on day 2 after the induction of adipogenesis in 3T3-L1 adipocytes. SET1A knockdown using siRNA in 3T3-L1 preadipocytes inhibited 3T3-L1 preadipocytes' adipogenesis, as assessed by Oil Red O staining and the expression of adipogenic genes, indicating that SET1A stimulates the adipogenesis of 3T3-L1 preadipocytes. SET1A knockdown inhibited the cell proliferation of 3T3-L1 cells during mitotic clonal expansion (MCE) via down-regulation of the cell cycle gene cyclin E1, as well as the DNA synthesis gene, dihydrofolate reductase. Furthermore, SET1A knockdown repressed peroxisome proliferator-activated receptor gamma ($PPAR{\gamma}$) expression during the late stage of adipogenesis. These results indicate that SET1A stimulates MCE and $PPAR{\gamma}$ expression, which leads to the promotion of 3T3-L1 preadipocytes' adipogenesis.

• Laboratory Medicine Online
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• v.6 no.1
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• pp.25-30
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• 2016

Background: The cell cycle-dependent enzyme thymidine kinase 1 (TK1) is known to increase during cancer cell proliferation and has been reported as a prognostic marker for various hematologic malignancies and solid tumors. This study aimed to determine the reference interval in Korean healthy controls and to evaluate the usefulness of TK1 as a biomarker for aggressive clinical behavior in B-cell lymphoma patients. Methods: We enrolled 72 previously untreated patients with B-cell lymphoma and 143 healthy controls. Serum TK1 levels were measured by chemiluminescence immunoassay ($Liaison^{(R)}$, DiaSorin, USA). We established the reference intervals in healthy controls. The diagnostic performance of serum TK1 was studied using receiver operating characteristic (ROC) analysis, and the correlation between the cutoff level for serum TK1 and clinical characteristics of B-cell lymphoma was evaluated. Results: The reference range (95th percentile) of serum TK1 in healthy controls was 5.4-21.8 U/L. There was a clear difference in TK1 levels between patients with B-cell lymphoma and healthy controls ($40.6{\pm}68.5$ vs. $11.8{\pm}4.4U/L$, P <0.001). The area under the curve of serum TK1 for the diagnosis of B-cell lymphoma was 0.73 (cutoff, 15.2 U/L; sensitivity, 59.7%; specificity, 83.2%). An increased TK1 level (${\geq}15.2U/L$) correlated with the advanced clinical stage (P <0.001), bone marrow involvement (P =0.013), international prognostic index score (P =0.001), lactate dehydrogenase level (P =0.001), low Hb level (<12 g/dL) (P =0.028), and lymphocyte count (P =0.023). Conclusions: The serum TK1 level could serve as a useful biomarker for aggressive clinical behavior in B-cell lymphoma patients.

• Journal of the Institute of Electronics Engineers of Korea SD
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• v.46 no.8
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• pp.95-101
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• 2009

Random numbers are used in many sorts of applications. Some applications, like simple software simulation tests, communication protocol verifications, cryptography verification and so forth, need various levels of randomness with various process speeds. In this paper, we propose a fast pseudorandom generator module for embedded systems. The generator module is implemented in hardware which can run in two modes, one of which can generate random numbers with higher randomness but which requires six cycles, the other providing its result within one cycle but with less randomness. An ASIP (Application Specific Instruction set Processor) was designed to implement the proposed pseudorandom generator instruction sets. We designed a processor based on the MIPS architecture,, by using LISA, and have run statistical tests passing the sequence of the Diehard test suite. The HDL models of the processor were generated using CoWare's Processor Designer and synthesized into the Dong-bu 0.18um CMOS cell library using the Synopsys Design Compiler. With the proposed pseudorandom generator module, random number generation performance was 239% faster than software model, but the area increased only 2.0% of the proposed ASIP.

• Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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• v.18 no.2_spc
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• pp.317-325
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• 2020

The Korea Radioactive Waste Agency plans to expand the storage capacity of radioactive waste by constructing a radioactive waste inspecting building to solve the problem of the lack of inspection space and drum-handling space in the radioactive waste receipt and storage building for the first-stage disposal facility. In this study, the exposure doses of radiation workers that handle new disposal containers for decommissioning waste in the storage areas of the radioactive waste inspecting building were calculated using the Monte Carlo N-particle transport code. The annual collective dose was calculated as a total of 84.8 man-mSv for 304 new disposal containers and an estimated annual 306 working hours for the radiation work. When the 304 new disposal containers (small/medium type) were stored in the storage areas, it was found that 25 radiation workers should be involved in acceptance/disposal inspection, and the estimated exposure dose per worker was calculated as an average annual value of 3.39 mSv. When the radiation workers handle the small containers in high-radiation dose areas, the small containers should be shielded further by increasing the concrete liner thickness to improve the work efficiency and radiation safety of the radiation workers. The results of this study will be useful in establishing the optimal radiation working conditions for radiation workers using the source term and characteristics of decommissioning waste based on actual measurements.

• Clinical and Experimental Reproductive Medicine
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• v.23 no.1
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• pp.103-108
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• 1996

Through the previous studies(I,II), it was observed that human follicular fluid(HFF) was more effective than human fetal cord serum(HFCS) on promoting meiotic resumption of oocytes and improving embryonic development of mouse in vitro. On the basis of these results, we have gradually exchanged HFCS with HFF as protein supplement in human ART. This study was performed to investigate the efficiency of HFF on improving the pregnancy rate in ART. Oocytes were retrieved transvaginally from patients treated with pituitary suppression with GnRH-agonist and ovarian stimulation with human menopausal gonadotro-pin(HMG) and pure follicle stimulating hormone(FSH). Aspirated oocytes were rinsed and cultured in TCM-199 containing HFF, and the concentrations of HFF were adjusted to 10, 20, and 30% according to the use for insemination, embryo growth and embryo transfer, respectively. As possible as, we tried to do embryo transfer into fallopian tube to mimic the coincidence of the cell stage with the place of sojourn in vivo, so we performed various ART programs(IVF & ET; in vitro fertilization, ZIFT; zygote intra fallopian-tube transfer, ZIFT & ET) according to the tubal conditions of patients. On the while, intra cytoplasmic sperm injection(ICSI) was used to assist IVF of the patients who had shown poor standard IVF results by immunological or severe male factor. Of the 255 cycles of ART programs using HFF as protein supplement, 118 cycles were turn out to be succeeded in pregnancy(46.2%, per cycle, p<0.05), while 21 pregnancies were achieved in the 69 cycles using HFCS(30.4%). The 255 cycles using HFF were subdivided into cycles with the type of ART programs, and each pregnancy rate of the ART programs were 44.7% (IVF & ET, 76/170 cycles), 53.4%(ZIFT, 31/58 cycles) and 40.7% (ZIFT & ET, 11/27 cycles), respectively. In the 61 ICSI cycles using HFF, 28 cycles succeed in pregnancy(45.9%), while 7 pregnancies were obtained in the 17 ICSI cycles using HFCS. Also the ongoing pregnancy rate in the group using HFF(78.8%, 93/118 cycles) was higher than that in the group using HFCS(61.9%). Therefore, we found that the use of HFF as protein supplement was more suitable and effective than the use of HFCS to improve the pregnancy rate in ART.

• Asia-Pacific Journal of Business Venturing and Entrepreneurship
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• v.1 no.2
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• pp.157-192
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• 2006

Since the late 1990s, the Korean Venture Capital Industry has been remarkably grown in the aspect of quality and quantity. Korean government expects that the Venture company and Venture Capital Industry would contribute to the recovery of depressed Korean economy and restructuring of the high cost and low efficiency economic structure. Korean government reinforces supporting policies for the Venture Capital and Venture Business. Venture Capital is defined as the form of high risk and high profit investment capital growing the small & medium enterprises to competitive ones through capital and management support and collecting the capital. According to the Gompers and Lerners the venture capital cycle consists of raising investment capital, screening the investment opportunity and invest the money. And later, sold the retained stock to the other investor or to the company. This stage called EXIT Consequently, the function of the venture capital, which supply the fund and the business consultation to venture business, have been emphasized and how to effectively run this capital have been recognized as the way to develop the venture business. In this regard, the problem in Korean Venture Capital Market is as follows. First, most of the sources of fund depends on the government support and this conflict with the nature of risk capital because the government capital emphasis the stability than profitability. And secondly, the efficiency of the venture capital system in Korea do not reach that of the advanced countries due to many kinds of restriction and the rack of support. Consequently, the Activation Schemes for Korean Venture Capital Firms are as follows. First, the sources of venture capital need to diversify from angels to institutional investors such as banks, pensions, fund of fund. And Lastly, the internal management and operational system of venture capital companies should be strengthened by quality to that of global Venture Capital Firms.

• The KIPS Transactions:PartD
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• v.8D no.1
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• pp.71-80
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• 2001

Object-Oriented (OO) methodology to use the concept like encapsulation, inheritance, polymorphism, and message passing demands metrics that are different from structured methodology. There are many studies for OO software metrics such as program complexity or design metrics. But the metrics for the analysis class need to decrease the complexity in the analysis phase so that greatly reduce the effort and the cost of system development. In this paper, we propose new metrics to measure the complexity of analysis classes which draw out in the analysis phase based on Unified Process. By the collaboration complexity, is denoted by CC, we mean the maximum number of the collaborations can be achieved with each of the collaborator and detennine the potential complexity. And the interface complexity, is denoted by IC, shows the difficulty related to understand the interface of collaborators each other. We prove mathematically that the suggested metrics satisfy OO characteristics such as class size and inheritance. And we verify it theoretically for Weyuker' s nine properties. Moreover, we show the computation results for analysis classes of the system which automatically respond to questions of the it's user using the text mining technique. As we compared CC and IC to CBO and WMC, the complexity can be represented by CC and IC more than CBO and WMC. We expect to develop the cost-effective OO software by reviewing the complexity of analysis classes in the first stage of SDLC (Software Development Life Cycle).

• Journal of the Institute of Electronics Engineers of Korea SD
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• v.48 no.10
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• pp.72-81
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• 2011

In this paper, we proposed and designed a novel floating point converter which supports single and double precisions of IEEE754 standard. The proposed convertor supports conversions between floating point number single/double precision and signed fixed point number(32bits/64bits) as well as conversions between signed integer(32bits/64bits) and floating point number single/double precision and conversions between floating point number single and double precisions. We defined a new internal format to convert various input types into one type so that overflow checking could be conducted easily according to range of output types. The internal format is similar to the extended format of floating point double precision defined in IEEE754 2008 standard. This standard specifies that minimum exponent bit-width of the extended format of floating point double precision is 15bits, but 11bits are enough to implement the proposed converting unit. Also, we optimized rounding stage of the convertor unit so that we could make it possible to operate rounding and represent correct negative numbers using an incrementer instead an adder. We designed single cycle data path and 5 cycles data path. After describing the HDL model for two data paths of the convertor, we synthesized them with TSMC 180nm technology library using Synopsys design compiler. Cell area of synthesis result occupies 12,886 gates(2 input NAND gate), and maximum operating frequency is 411MHz.

• Korean Journal of Construction Engineering and Management
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• v.12 no.3
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• pp.42-52
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• 2011

As environmental issues are rising recently efforts to reduce environmental stress are emerging in all industry segments. Especially environmental impact of buildings occupy a critical portion, so each country is operating green building rating system for life cycle of buildings. Accordingly green building rating system for every facility is operating in Korea. And acquisition of grade I for building energy efficiency is mandatory for every new public buildings since 2010. To design green building efficiently and systematically eco-friendly elements should be considered and checked from the schematic design phase. But in many cases eco-friendly elements are checked at the end of constructed design phase. So applying eco-friendly elements at the value engineering process, which is performing through schematic and constructed design phase, could make a efficient and systematic green building design. Value engineering process is divided into pre workshop, workshop and post workshop stages. And subject selection in pre workshop stage is the step that finds out the subjects which has the great possibility to be improved to perform efficient value engineering workshop. So this study present the Green VE subject selection model to select the most considerable eco-friendly subjects in projects.

• Journal of Engineering Education Research
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• v.11 no.1
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• pp.64-75
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• 2008

A new product development and product realization process (PRP) is a circulating and feedback process by generating ideas through creative problem solving process. The early stages of PRP correspond to conceptual design and product development, in which a large portion of product life cycle cost can be saved. The optimal design method for this stage is a robust design suggested by Taguchi. Quality must be 'engineered in' since it can not be 'inspected out.' A robust design is an engineering methodology to improve the quality of a product by minimizing the efforts of variations without eliminating the causes. The objective of this study is to propose a scheme and a case study of robust design for exploring design parameters, and introduce a creative problem solving process. Major research subjects include a creative problem solving process, robust design procedure and their implementation. For the experiment of Taguchi method, a toy catapult is adopted. For the creativity development, a short project is assigned to devise a similar tool with the toy catapult. A reference model is suggested to compare and evaluate their ideas.