• Nuclear Engineering and Technology
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• 제54권1호
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• pp.19-29
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• 2022

The 2D/1D method has become the mainstream of the direct transport calculation considering the balance of accuracy and efficiency. However, the 2D/1D method still suffers from stability issues. Recently, a quasi-3D method has been proposed with axial Legendre expansion. Analysis and comparison of the 2D/1D and quasi-3D method is conducted in theory from the equation derivation. Besides, the C5G7 benchmark, the KUCA benchmark and the macro BEAVRS benchmark are calculated to verify the theory comparisons of these two methods with the direct transport code SHARK. All results show that the quasi-3D method has better stability and accuracy than the 2D/1D method with worse efficiency and memory cost. It provides a new option for direct transport calculation with the quasi-3D method.

• 한국연소학회:학술대회논문집
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• 한국연소학회 2015년도 제51회 KOSCO SYMPOSIUM 초록집
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• pp.113-114
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• 2015

In this study, 1D and 3D thermoacoustic analysis model were developed in order to predict fundamental characteristics of combustion instability in a gas turbine lean premixed combustor. The 1D network model can be used to analyze frequency and growth rate of combustor instability by simply dividing whole system into a couple of acoustic sub-elements, while the 3D Helmholtz solver model can predict directly acoustic modes as well as basic properties of combustion instability. Prediction results of both 1D and 3D models generally showed a good agreement with the measurements, even if there was a slight overestimation for instability range.

• 대한수학회논문집
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• 제9권2호
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• pp.423-438
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• 1994

We employ the Galerkin method to solve the nonlinear Urysohn integral equation (1.1) x(t) = f(t) ＋ $∫_{D}$ k(t, s, x(s))ds (t $\in$ D), where D is a bounded domain in $R^{d}$ , the function f and k are known and x is the solution to be determined. We assume that D has a locally Lipschitz boundary ([1, p. 67]). We can rewrite (1.1) in operator notation as x = f ＋ Kx. We consider (1.1) as an operator equation on $L_{\infty$}$(D) and assume that K is defined on the closure $\Omega$ of a bounded open set $\Omega$ ⊂ $L_{\infty}$(D). Throughout our analysis we put the following assumptions on (1.1).(omitted)(1.1).(omitted)

• 대한의생명과학회지
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• 제27권2호
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• pp.105-110
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• 2021

Short Tandem Repeats (STR) analysis which characterized by genetic polymorphism has been widely used in the forensic genetic fields. Unfortunately, mutation occurred in various STR loci could make it difficult to interpret STR data. Thus, the mutation rate of STR loci plays an important role for the data interpretation in human identification and paternity test. To verify the mutation of the STR loci in the Korean population, 545 trio sets (father, mother, and child) were analyzed with two commercial STR kits that include the 23 autosomal STR loci (D1S1656, TPOX, D2S441, D2S1338, D3S1358, FGA, D5S818, CSF1PO, D7S820, D8S1179, D10S1248, TH01, D12S391, VWA D13S317, D16S539, D18S51, D19S433, D21S11, D22S1045, SE33, Penta E and Penta D). As a result, 36 mutations were observed in 14 STR loci. The types of mutation were also classified by the increase or decrease of the alleles. The overall mutation rate was 1.4×10^-3, and the paternal mutation rate was four times higher than that of the maternal. This study will provide more detailed criterion for human identification by the mutation rate of STR loci in the Korean population.

• International Journal of Control, Automation, and Systems
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• 제3권1호
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• pp.56-69
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• 2005

In this paper, it is clearly shown that the two well-known necessary and sufficient conditions mp n as generic static output feedback pole-assignment and mp + d(m+p) n+d as generic minimum d-th order dynamic output feedback pole-assignment on complex field, unbelievably, do not match up each other in strictly proper linear systems. For the analysis, a diagram analysis is newly created (which is defined by the analysis of 'convoluted rectangular/dot diagrams' constructed via node-branch conversion of the signal flow graphs of output feedback gain loops). Under this diagram analysis, it is proved that the minimum d-th order dynamic output feedback compensator for pole-assignment in m-input, p-output, n-th order systems is quantitatively decomposed into static output feedback compensator and its associated d number of arbitrary 1st order dynamic elements in augmented (m+d)-input, (p+d)-output, (n+d)-th order systems. Total configuration of the mismatched data is presented in a Table.

• 미생물학회지
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• 제27권1호
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• pp.35-42
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• 1989

The mode of transglycosylation reaction observed during the action of low-molecular-weigh $\beta$-D-glucosidase ($\beta$-D-glucoside glucohydrolase, EC3.2.1.21) purified from Trichoderma koningii ATCC 26113 was investigated using $^{1}H$-NMR spectroscopy. The enzyme was purified by the series of procedures including ammonium sulfate precipitation, and fractionations by column chromatographies on Bio-Gel P-150, DEAE-Sephadex A-50, and SP-Sephadex C-50. The final purification was performed by the band eluation after preparative polyacrylamide gel electrophoresis. The enzyme showed its molecular size of 78,000 through the analysis of sodium dodecyl sulfate-polyacrylamide gel electrophoresis and its isoelectric point of 5.80 through the analysis of analytical isoelectric focusing. The H-1 proton resonances were analyzed. After the reaction of the enzyme with cellobiose, the reaction products were separated by high performance liquid chromatography using refractive index detector. H-1 resonances of the products were consisted with those of gentiobiose [$\beta$-D-glucopyranosyl--(1,6)-D-glucopyranose], and cellotriose [$\beta$-D glucopyranosyl-(1,4)-$\beta$-D-glucopyranosyl]-(1,4)-D-glucopyranose] with minor resonances of sophorose [$\beta$-D-glucopyranosyl-(1,2)-D-glucopyranose], respectively.

• Korean Chemical Engineering Research
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• 제58권1호
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• pp.106-112
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• 2020

Analyzing vitamin D levels is important for monitoring health conditions because vitamin D deficiency is associated with various diseases such as rickets, osteomalacia, cardiovascular disorders and some cancers. However, vitamin D concentration in the blood is very low with optimal level of 75 nmol/L, making quantitative analysis difficult. The objective of this study was to develop a highly sensitive analysis method for vitamin D using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-MS). 25-hydroxyvitamin D (25(OH)D), which has been used as an indicator of vitamin D metabolites in human biofluids was chemically derivatized using a secosteroid signal enhancing tag (SecoSET) with powerful dienophile and permanent positive charge. The SecoSET-derivatized 25(OH)D provided good linearity (R² > 0.99) and sensitivity (limit of quantitation: 11.3 fmol). Chemical derivatization of deuterated 25-hydroxyvitamin D₃ (d₆-25(OH)D3) with SecoSET enabled absolute quantitative analysis using MALDI-MS. The highly sensitive method could be successfully applied into monitoring of quantitative changes of bioactive vitamin D metabolites after treatment with ketoconazole to inhibit 1α-hydroxylase reaction related to vitamin D metabolism in human breast cancer cells. Taken together, we developed a MALDI-MS-based platform that could quantitatively analyze vitamin D metabolites from cell products, blood and other biofluids. This platform may be applied to monitor various diseases associated with vitamin D deficiency such as rickets, osteomalacia and breast cancer.

• 지질공학
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• 제33권1호
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• pp.151-167
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• 2023

본 연구는 LNG저장탱크가 설치될 느슨한 포화사질지반을 대상으로 개정된 액상화 평가법과 UBC3D-PLM 모델을 이용한 1차원 유효응력해석에 의한 액상화 평가법을 비교한 것이다. 이를 위해 여러가지 실내 및 현장시험을 실시하여 필요한 Parameter를 산정하였다. 검토결과, 지진응답해석결과와 SPT N 값을 이용하는 개정 액상화 평가법은 액상화 발생 가능성을 높게 평가하였지만, 다양한 액상화 저항인자를 고려할 수 있는 유효응력해석법은 액상화에 다소 안정한 것으로 분석되었다. UBC3D-PLM 모델을 이용한 1차원 유한요소해석을 할 경우 보다 간편하게 액상화 안정성 검토가 가능하였고, 액상화 보강 영역을 최소화 할 수 있었다. 또한, LNG저장탱크의 기초를 고려한 2·3차원 수치해석 시에는 액상화 발생 시 내진설계 및 거동특성을 규명하는 것에 활용될 수 있을 것으로 기대된다.

• 생약학회지
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• 제50권1호
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• pp.59-64
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• 2019

Cudrania tricuspidata Bureau (Moraceae) has been used for physical weakness, impotence and insomnia in traditional Korean medicine. In this study, cudratricusxanthone A and cudraxanthone D were isolated from roots of C. tricuspidata and quantification were achieved by using high performance liquid chromatography (HPLC) method with diode array detector. The isolated compound was identified by NMR analysis and HPLC method was validated by linearity, precision and specificity test. The results showed that calibration curves of two compounds indicated great linearity with a correlation coefficient ($R^2$) of 0.9976 and 0.9995. The limits of detection (LOD) for cudratricusxanthone A and cudraxanthone D were 1.15, $0.11{\mu}g/ml$. Intra-day precision of cudratricusxanthone A and cudraxanthone D were 0.32~1.99%, 0.09~3.32% and inter-day precision were 0.59~2.40%, 0.28~1.55% RSD (%) values, respectively. The specificity was confirmed by chromatograph and quantitative contents of cudratricusxanthone A and cudraxanthone D were $0.46{\pm}0.02%$ and $1.53{\pm}0.06%$. Therefore, this study can be used as a basic research data for the quantitative analysis of derived compounds from roots of C. tricuspidata.

• 대한의생명과학회지
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• 제24권4호
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• pp.430-434
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• 2018

Candida glabrata is the second most prevalent causative agent for candidiasis following C. albicans. The opportunistic yeast, C. glabrata, is able to cause the critical bloodstream infections in hospitalized patients. Conventional identification methods for yeasts are often time consuming and labor intensive. Therefore, recent studies on sequence-based identification have been conducted. Recently, sequencing the D1/D2 domain of the large subunit ribosomal RNA gene and the internal transcribed spacers (ITS) 1 and ITS2 regions of the ribosomal DNA has proven useful for DNA-based identification of most species of fungi. In the present study, therefore, fungal ITS and D1/D2 domain regions were targeted and analyzed by DNA sequencing for the accurate identification of C. glabrata clinical isolates. A total of 102 C. glabrata clinical isolates from various clinical samples including bloodstream, catheterized urine, bile and other body fluids were used in the study. The results of the DNA sequence analysis showed that the mean standard deviation of species identity percent score between ITS and D1/D2 domain regions was $97.8%{\pm}2.9$ and $99.7%{\pm}0.46$, respectively. These results revealed that the D1/D2 domain region might be a better target for identifying C. glabrata clinical isolates based on DNA sequences than the ITS1 and ITS2 regions. However, in order to evaluate the usefulness of D1/D2 domain region for species identification of all Candida species, other Candida species such as C. albicans, C. tropicalis, C. dubliniensis, and C. krusei should be verified in further studies additionally.