• 생명과학회지
• /
• 제13권3호
• /
• pp.265-272
• /
• 2003

동충하초(C. militaris)의 균사체 액체배양액이 고지방 식이를 섭취한 성숙한 암쥐의 생리활성에 미치는 영향을 평가하고자, 생후 30주령의 흰쥐에 표준식이를 급여한 정상군, 표준식이에 15% 돈지와 1% 콜레스테롤을 첨가한 식이를 급여한 고지방군, 고지방 식이에 균사체 액체배양액을 각각 10%, 20% 및 30%씩 음료수에 혼합하여 급여한 군 등 5군으로 나누어 5주간 사육하였다. 실험동물의 체중증가, 식이효율, 및 간의 무게는 고지방군과 각 수준별 균사체 배양액군이 비슷한 수준을 보였으나 정상군 보다는 유의하게 증가되었다. 간의 총 지질 농도, 간과 혈청의 중성지질 농도 및 동맥경화지수는 고지방군에 비하여 20%와 30% 균사체 배양액군이 유의하게 감소시키는 효과가 나타났다. 혈청의 HDL-콜레스테롤 농도는 고지방군에 비하여 각 수준별 균사체 배양액군이 유의하게 증가시키는 효과가 나타났다. 분중 중성지질 배설량은 고지방군에 비하여 20%와 30% 균사체 배양액군이 유의하게 증가되었다. 간과 혈청의 총 콜레스테롤 농도, 혈청의 LDL-콜레스테롤 농도, 분의 콜레스테롤 배설량, 및 혈청의 GPT와 ALP의 활성은 고지방군과 각 수준별 균사체 배양액군이 비슷한 경향으로 나타났으나 정상군 보다는 유의하게 증가되었다. 총 콜레스테롤에 대한 HDL-콜레스테롤 비율은 정상군에 비하여 각 실험군이 유의하게 감소되었다. 혈당, 혈청의 총 단백질과 알부민 농도, 알부민/글로불린 비율, 신장과 대퇴골의 무게, 혈청의 COT와 ${\gamma}$-CTP의 활성은 정상군과 각 실험군이 비슷하여 정상 수준을 유지하였다. 이상의 결과로 보아 고지방 식이에 20% 및 30% 동충하초의 균사체 액체배양액 섭취가 간의 총 지질, 간과 혈청의 중성지질 농도 및 동맥경화지수를 낮추는 효과가 있고, HDL- 콜레스테롤 농도를 증가시키는 효과가 있는 것으로 나타났다.각된다.

• Nutrition Research and Practice
• /
• 제4권2호
• /
• pp.114-120
• /
• 2010

The effects of grape seeds extract and grape peels extract prepared from grape pomace on the activity of antioxidant enzymes, degree of lipid peroxidation in serum and liver tissue were investigated in rabbits fed on high cholesterol diet. New Zealand white rabbits were divided as follows ; 1) NOR (normal group); 2) CHOL (cholesterol group); 3) GSH (cholesterol + grape seed extract group); 4) GPE (cholesterol + grape peel extract); 5) GSP (cholesterol + grape seed powder); 6) GPP (cholesterol + grape peel powder); 7) GE (cholesterol + grape seed and peel extract); 8) GP (cholesterol + grape seed and peel powder). Eight groups of rabbits were studied for 8 weeks. At the end of the experimental period, rabbits were sacrificed and the liver tissue were removed. Then, GSH, GPx, GST, CAT and MDA in the liver were measured. In liver tissues, total glutathione contents (GSH), glutathione peroxidase (GPx) and catalase (CAT) activity, which was significantly higher by grape seed extract supplementation. The level of malondialdehyde (MDA) was lower in the serum of rabbits fed grape seed extract or grape peel powder plus cholesterol than in the serum of rabbits fed cholesterol alone. It is therefore likely that grape seed extract prepared from grape pomace functioned as antioxidants in vivo, negating the effects of the oxidative stress induced by 1% cholesterol diet. The grape seed extract was found effective in converting the oxidized glutathione into reduced glutathione, and in removing $H_2O_2$ that is created by oxidative stress. The grape peel powder was found to have small influence on reduced glutathione content, CAT and GPX activity, but it increased GST activity in liver tissues, resulting in promoting the combination of lipid peroxide and glutathione (GSH), and further, lowering the formation of lipid peroxide in the serum. Therefore, grape pomace (grape seed extract and grape peel powder) supplementation is considered to activate the antioxidant enzyme system and prevent damage with hypercholesterolemia.

• 한국식품위생안전성학회지
• /
• 제20권1호
• /
• pp.69-72
• /
• 2005

이 연구는 글루코만난과 유청칼슘의 혼합물이 $1\%$-Cholesterol을 식이한 후 혈청콜레스테롤에 미치는 영향과 $70\%$-Sucrose를 식이한 후 혈당에 미치는 영향을 알아보았다. $1\%$-Cholesterol을 식이한 쥐를 일반식이군(ND) 비교하였으며, 고콜레스테롤식이군(HCD)에 글루코만난식이군(HCDG)과 글루코만난과 유청칼슘군식이군(HCDGW)으로 나누어 관찰하였다. 또한 $70\%$-Sucroe를 식이한 쥐를 일반식이군(ND) 비교하였으며, 고혈당식이군(HGD)에 글루코만 난식이군(HGDG)과 글루코만난과 유청칼슘군식이군(HCDGW)으로 나누어 관찰하였다. 이를 비교 조사한 결과를 요약하면 다음과 같다. 1. 혈청콜레스테롤수치는 HCD>HCDG>HCDGW>ND의 순서로 감소하였다. 2. HCD의 혈청콜레스테롤증가량에 비하여HCDC는 약 $23.84\%$ HCDCW는 약 $42.38\%$ 감소하였다. 3. 혈당수치는 HGD>HGDG>HGDGW>ND의 순서로 감소하였다. 4. HGD의 혈당증가량에 비하여 HGDG는 약 $6.63\%$ HGDGW는 약$36.14\%$ 감소하였다.

• Journal of Nutrition and Health
• /
• 제40권5호
• /
• pp.463-474
• /
• 2007

This study attempted to evaluate the effectiveness of nutrition education especially high nutrient density diet, which promotes low carbohydrate, high protein and fiber. Sixty nine college students participated in the 8 week weight management program with nutrition education. After the program, forty six experienced a small amount of weight loss (WL group, 1.3 kg), but twenty three did not (WG group). The WL group's dietary habits and diet quality improved significantly. The INQ of nutrients and MAR significantly increased only in the WL group. The total DQI-I score significantly increased from 71.1 to 75.3 in the WL group, but it did not in the WG group. The total dietary habit scores significantly increased in both groups, but the changes in the dietary habit scores were greater than the WG group in the WL group. After the program, total cholesterol and triglyceride level decreased significantly in the WL group (p < 0.05). These results show that nutrition education which focuses on a nutrient density diet could help improve dietary habits, diet quality, total cholesterol, and the triglyceride level in college women.

• 대한본초학회지
• /
• 제28권3호
• /
• pp.95-106
• /
• 2013

Objectives : The researcher investigated the anti-obesity effect of Alismatis Orientale Rhizoma(AP) water extract in mice fed a high fat diet and focused on the analysis of local area adipose tissue. Methods : Male ICR mice were divided into three groups, which were fed either a normal AIN diet, a 45% high fat diet (CT group), or a high fat diet and orally administration with a concentrations of 100 mg/kg (AP100 group) and 300 mg/kg body weight (AP300 group) for eight weeks. Results : As compared with CT group, AP100 group showed significant reductions in absolute weight of liver. As compared with CT group, AP100 group and AP300 group showed significant reductions in weight gain and relative weight of total fat. AST, triglyceride, total-cholesterol levels from the AP 100 group and 300 group were significantly lower than those of the CT, and ALT, LDL-cholesterol levels from the AP 100 group was significantly lower than those of the CT. But serum HDL-cholesterol levels from the AP 100 group and 300 group were significantly higher than those of the CT. And serum adiponectin levels from the AP 100 group was significantly higher than those of the CT. In result of real time PCR, all mRNA expression(PEPCK, G6Pase, PGC-$1{\alpha}$ and $ERR{\gamma}$) of two experimental groups were significantly decreased compared to those of CT group. The treatment with AP on local abdominal area made a fat cell size lessen on the fat tissue in the abdominal cavity and subcutaneous area. Conclusions : These results suggest that AP has an anti-obesity effect and the effect is mediated by inhibition of fat gain.

• 생명과학회지
• /
• 제9권4호
• /
• pp.389-394
• /
• 1999

Male mice (ddY strain) were fed a laboratory chow diet containing 10% sucrose supplemented with orotic acid, hesperetin or naringenin at the 1% level for 14 days. the concentrations of liver triacylglycerol and cholesterol were significantly lower in the OA group than in the control group. When both flavonoids and orotic acid were administered simultaneously, the orotic acid-dependent decrease in liver triacylglycerol and cholesterol were attenuated slightly. The concentration of serum cholesterol in the orotic acid group or the control group was lower than in the orotic acid groups supplemented with hesperetin or naringenin. There were no significantly difference in body weight gain, diary food intake, and the serum concentrations of triacylglycerol and high-density-lipoprotein cholestrol. It was concluded that the inducement of fatty liver in mice failed to feeding a laboratory chow diet containing 10% sucrose supplemented with 1% orotic acid for 14 days.

• Journal of Nutrition and Health
• /
• 제56권6호
• /
• pp.641-654
• /
• 2023

Purpose: Sour cherry (Prunus cerasus L.) contains abounding phytochemicals, such as polyphenols and anthocyanins, and has antioxidative effects. Adenosine monophosphate-activated protein kinase (AMPK) is a crucial regulator in enhancing the lipid metabolism. This study hypothesized that the intake of sour cherry affects AMPK signaling. Therefore, this study examined whether sour cherry regulates AMPK to balance the hepatic lipid metabolism and exert ameliorating effects. Methods: Male C57BL/6J mice had obesity induced with a 45% fat diet. The mice were divided into four groups: control (CON), high-fat diet (HFD), low percentage sour cherry powder (LSC), and high percentage sour cherry powder (HSC). The mice in the sour cherry groups were fed 1% sour cherry or 5% sour cherry in their respective diets for 12 weeks. Results: The body weight, visceral fat weight, and lipid droplet size significantly decreased in the treatment groups. The serum and hepatic triglyceride and total cholesterol levels improved significantly in the HSC group. The low-density lipoprotein cholesterol levels were also reduced significantly, whereas the high-density lipoprotein cholesterol levels were increased significantly in both treatment groups. The sterol regulator binding protein-1c and fatty acid synthase expression levels as fatty acid synthesis-related enzymes were significantly lower in the treatment groups than in the high-fat diet group. Furthermore, the adipose triglyceride lipase and hormone-sensitive lipase expression levels as lipolytic enzyme activity and AMPK/acetyl-CoA carboxylase/carnitine palmitoyltransferase-1 as fatty acid β-oxidation-related pathway were upregulated significantly in both sour cherry groups. Conclusions: These results show that sour cherry intake improves hepatic lipid synthesis and chronic diseases by activating AMPK signaling. Therefore, this study suggests that phytochemical-rich sour cherry can be developed as a healthy functional food.

• 생약학회지
• /
• 제32권2호통권125호
• /
• pp.145-152
• /
• 2001

Whangryunhaedok-Tang (WT) is formulated with Coptidis Rhizoma, Phellodendri Cortex, Scutellariae Radix and Gardeniae Fructus, and Daewhang-whangryunhaedok-Tang (DWT) is made by the combination of Rhei Rhizoma, a wellknown anticostipation drug in WT. Therefore, DWT has been evaluated for antihyperlipidemic effects on experimental hyperlipidemic rats and mice induced by corn oil and high cholesterol-diet. Oral administration of DWT significantly inhibited the increase of serum triglyceride and LDL-cholesterol levels, and the decrease of serum HDL-cholesterol levels in hyperlipidemic rats induced by corn oil. Also, oral administration of DWT significantly prevented the increase of serum total cholesterol, triglyceride and LDL-cholesterol, and liver total cholesterol and triglyceride in 1% cholesterol-diet fed mice. These results suggest that DWT is effective for the treatment of hyperlipidemia.

• 생약학회지
• /
• 제34권2호통권133호
• /
• pp.179-184
• /
• 2003

We have investigated the effect of MeOH extract of the adventious root culture of Panax ginseng on hyperlipidemic rats induced by fat-rich diet. We also analyzed and compared the content ginsenoisdes of the cultured ginsengs by HPLC. After oral administration of the MeOH extract to hyperlipidemic rats for four weeks, the variables including body weight, cholesterol, HDL, LDL, and triglyceride levels in serum were measured. One of the cultured ginseng roots (CBN3) decreased cholesterol and LDL-cholesterol and increased HDL-cholesterol levels in serum. HPLC analysis demonstrated that CBN3 contains more $Rb_1$ and Rd ginsenosides than any other cultured ginsengs and cultivated ginseng. These results suggest that CBN3 with high contents of $Rb_1$ and Rd may be useful in lowering blood cholesterol.

• Preventive Nutrition and Food Science
• /
• 제18권3호
• /
• pp.157-162
• /
• 2013

The purpose of this study was to investigate the effects of the ethanol extract of Cynanchum wilfordii (ECW) on the blood lipid profile of hypercholesterolemic rats. Thirty 7-week-old male Sprague-Dawley rats were allowed free access to either a normal diet (AIN-93 diet), or 1% high-cholesterol diet with or without 0.5% or 1% ECW for 5 weeks. After sacrifice, the rat serum lipid profile was analyzed. The diets containing ECW decreased body weight gains compared to the normal diet. Serum HDL-cholesterol levels of ECW-fed groups were significantly increased in the hypercholesterolemic groups and normal groups (P<0.05). When 1% ECW was fed to the normal group, total cholesterol level was increased. Moreover, treatment of ECW in hypercholesterolemic groups yielded a dose-dependent and highly significant decrease in the atherogenic index as compared to the control. These results suggest that intake of Cynanchum wilfordii may help reduce the risks of hypercholesterolemia by increasing blood HDL-cholesterol and lowering the atherogenic index.