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Hangover relieving effect of Sanghwang mushroom mycelium cultured in germinated buckwheat

  • An, Yoo-Jin;Cho, Sung-Min;Kim, Min-Su;Moon, Hae-Hee;Park, Dong-Soo;Jeon, Nam-Gen;Lee, oungjae;Han, Chang-Hoon
    • Korean Journal of Veterinary Research
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    • v.57 no.3
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    • pp.147-154
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    • 2017
  • The present study was performed to evaluate the hangover relieving effect of germinated buckwheat (GB) and Sanghwang mushroom mycelium cultured in GB (SGB). Both GB and SGB showed 1,1-diphenyl-2-picrylhydrazyl radical scavenging activities and significantly increased (p < 0.001) aldehyde dehydrogenase (ALDH) activities; up to 140% increase at concentrations of $16{\mu}L/mL$. Locomotor activity test results from alcohol-SGB and alcohol-GB groups showed improved motor activities over that of the alcohol-water group at 90 min post-administration. Both alcohol-GB and alcohol-SGB groups had significantly reduced (p < 0.001) alcohol ($40.02{\pm}33.38{\mu}g/mL$, $66.01{\pm}22.04{\mu}g/mL$, respectively) and aldehyde ($5.72{\pm}0.47{\mu}g/mL$, $6.72{\pm}1.70{\mu}g/mL$, respectively) concentrations in blood compared to those in the alcohol-water group ($199.75{\pm}33.83{\mu}g/mL$, $50.43{\pm}13.88{\mu}g/mL$, respectively) at 90 min post-administration. Based on cDNA microarray analysis, expressions of ALDH genes ALDH1a7 and ALDH18a1 and cytochrome P450 (CY450) gene CYP4a30b were upregulated in the alcohol-GB and alcohol-SGB groups compared to levels in the control group. Overall, the results suggest that both GB and SGB have hangover relieving effects by reducing blood acetaldehyde levels. The molecular mechanisms may involve ALDH activation and upregulated expression of alcohol metabolism-related genes such as ALDH and CYP450.

Studies on Evaluation of Spent Bleaching Clay as a Feed Ingredient in Broiler Chicks (육계의 사료원으로 식용유 정제 폐백토(Spent Bleaching Clay)의 평가에 관한 연구)

  • Jeon, H.Y.;Son, J.H.
    • Korean Journal of Poultry Science
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    • v.34 no.2
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    • pp.99-104
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    • 2007
  • Studies were conducted to evaluate spent bleaching clay as a feed resource. Raw spent bleaching clay from the soybean refining process in a rotary reactor is rich in crude fat (26.0%) and crude ash (68.3%) but low in moisture (5.2%) and crude protein (0.5%). The peroxide value of spent bleaching clay was significantly higher than soybean oil (P<0.05). Also the acid value of spent bleaching clay and soybean oil were 9.72 and 0.001, respectively, it was significantly different(P<0.01). It is possible that the acidification of spent bleaching clay was prevented by artificial method. On the feeding experiment, 2.0 to 4.0% of dietary spent bleaching clay were improve body weight gain, feed intake and feed/ gain. Results indicate that spent bleaching clay may be used at 2.0 to 4.0% for broiler diets without deleterious effects on performance.

Variation of Chamazulene Contents in Local Collections of Mugwort (Artemisia spp.) (수집종 쑥의 청색색소 카마줄렌 함량 변이)

  • Kim, H.J.;Chae, Y.A.
    • Korean Journal of Medicinal Crop Science
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    • v.9 no.3
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    • pp.181-185
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    • 2001
  • This study was carried out to select chamazulene containing species and to know whether chamazulene content varies depend on individual plants within a population or not. Among five collected mugworts, chamazulene was detected only in Artemisia indica. Chamazulene content in the leaves was higher than that in stems at vegetative stage. Camazulene in inflorescence was not higher than that in leaves even at reproductive stage. Chamazulene was higher in the leaves of the upper part of the plant than those of the middle and lower of the plant at vegetative stage. Chamazulene contents of 50 individuals within a population were varied from 0.01% to 0.11% with mean of 0.045%. About 36 individuals, 72 percent of total individuals examined, had more than 0.03% of chamazulene. There was no difference in chamazulene contents between parents and their clones.

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Chemosystematic and Phenotypic Characterization of Gram-positive Bacteria from Coastal Seawater, Korea (한국 근해에서 분리한 그람양성 세균의 화학 분류학적 및 표현형적 특성)

  • 전정훈;박진숙
    • Korean Journal of Microbiology
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    • v.36 no.3
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    • pp.167-172
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    • 2000
  • Twenty-five halotolerant gram-positlve bacteria were isolated from the coastal seawater 01 Cheju Island and Incheon J&yakdo Chemosystematic and phenotypic characteristics were used to iuvestigate the taxonomic position of these bacteria. According to their chemosystematic characteristics, the twenty-tive isolates were divided into 4 groups. Group 1 bacteria possesed 40.1 to 49.9 inol% m DNA G+C content, menaquinone-7 as a major quinone, and meso-Alpm as a diamino acid of peptidoglycan. Group 1 tam were identified as Bacilluspumilis, Bacillus lichenifbrrizis, Bacillus megaterium, Bncill~rsubtilis. Group 2 bacteria possessed 63.9 to 66.4 mol% and MK-8. They were all in the genus Arth~obaclm-. Group 3 bacteria possessed 31.0 to 37.6 mol% and MK-7. They were identified as Staphylococcus haeniol.viicvs, Siaph~~lococc~is sapropl~j~ticns, and Siaphylococcus intermediru.. Group 4 bacterium possessed 72.0mol% and MK-8 and was identified as ~Lficrococcus ltdtezm. Bacillus species accounted for 68% of h e total isolates.

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The Health Related Major College Students' Nutrition Knowledge and Practices toward Dietary Fat (건강 관련 전공 대학생의 지방에 대한 영양 지식과 실천도)

  • Won, Hyang-Rye
    • The Korean Journal of Food And Nutrition
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    • v.20 no.3
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    • pp.317-324
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    • 2007
  • This study examined students' nutritional knowledge on fat, the quantity of fat in food, and their dietary practices with regard to fat. The subjects were junior & senior students majoring in food and nutrition(n=188), Korean Oriental medicine(n=236), and physical education(n=13), respectively. The score for nutritional knowledge of fat was $8.48{\pm}0.15$ for food & nutrition majors, $6.73{\pm}0.14$ for Korean Oriental majors, and $4.97{\pm}0.72$ for physical education majors, with significant differences between groups(p<0.0l). The correct answer percentages were 65%, 52%, and 38% respectively, out of a total 13 items, and significant differences were observed between the majors. The score for knowledge on the quantity of fat in food was $5.40{\pm}01.3$ for food & nutrition majors, $5.40{\pm}0.10$ for Korean Oriental medicine majors, and $4.53{\pm}0.15$ for physical education majors, with significant differences between the groups. The correct answer percentages were 60%, 60%, and 50% respectively. The number of correct answers was significantly different in 7 out of a total of 9 food items depending on major. For dietary practices regarding fat there were significant differences in 10 out of a total of 12 items depending on major. The total scores were $34.02{\pm}0.45$, $33.04{\pm}0.44$, and $31.19{\pm}0.72$, respectively, showing no significant differences between the groups. Regarding the scores on nutritional knowledge about fat, the students majoring in food & nutrition received the highest scores, and on knowledge about the quantity of fat in food, students majoring in food & nutrition and Korean Oriental medicine attained higher scares than the students majoring in physical education. However, there were no significant differences between the groups regarding their dietary practices toward fat.

Changes of the blood chemistry, lipid and protein components in blood and liver tissue of the rat after oral combined administration of caffeine, iron and vitamin E (Caffeine, 철분 및 vitamin E 혼합투여시 rat의 혈액과 간조직내에서 혈액화학성분과 지질 및 단백질 구성성분의 변화)

  • Do, Jae-cheul;Huh, Rhin-sou
    • Korean Journal of Veterinary Research
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    • v.36 no.3
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    • pp.577-598
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    • 1996
  • This study was conducted to identify the effects of caffeine or combinations of caffeine and iron or vitamin E on the lipid and protein components or blood chemistry levels of the serum as well as the total homogenate, mitochondrial and microsomal fraction of the rat(Sprague-Dawley, female) liver. Chronic test were conducted to determine those effects. The chronic test was conducted by dividing rats into 5 groups according to the type of drugs and dosages administrated as follows; the control(group A), and group B was given 25mg/kg caffeine orally once daily for 30 days, group C was given 50mg/kg caffeine orally once daily for 30 days, group D was given 25mg/kg caffeine and orally ferric chloride once daily for 30 days and group E was given 25mg/kg caffeine and 25mg/kg vitamin E once daily for 30 days. The concentrations of glucose, urea nitrogen, uric acid, creatinine, total protein, albumin, A/G ratio, triglyceride, total cholesterol, HDL-cholesterol, free fatty acid, phospholipid as well as the activities of alanine aminotransferase(ALT), aspartate aminotransferase(AST) and alkaline phosphatase(ALP) were measured in the serum of each experimental groups. The concentrations of the carbonyl group and malondiaidehyde(MDA) and the patterns of the SDS-PAGE(Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis) and fatty acid compositions in free fatty acids and phospholipids were analyzed to determine the oxidative damages and metabolic changes on the lipid and protein components in the serum, and total homogenate, mitochondrial and microsomal fractions of the rat liver. The results obtained from this study were summarized as follows; 1. Body weights of groups B, C, D and E were significantly decreased(p < 0.01) in comparison with that of the control in the chronic test. 2. The concentrations of serum glucose in groups B(124.5mg/dl), C(130.1mg/dl), D(122.1mg/dl), E(119.3mg/dl) were significantly higher(p < 0.01) in comparison to that of the control(101.5mg/dl). But, there were no significant differences in the concentrations of urea nitrogen, uric acid, creatinine, total protein, albumin and A/G ratio in comparison to that of the control. 3. The concentrations of total cholesterol and HDL-cholesterol in serum of groups B(69.6, 53.4mg/dl), C(73.0, 56.3mg/dl), D(68.9, 51.1mg/dl) and E(68.2, 51.3mg/dl) were significantly higher(p < 0.01) in comparison to that of the control(52.6, 38.8mg/dl). On the other hand, the concentrations of triglyceride in serum of groups B(45.0mg/dl), C(40.4mg/dl), D(33.8mg/dl) and E(47.2mg/dl) were significantly lower(p < 0.01) in comparison to that of the control(66.2mg/dl). There were no significant differences in the activities of ALT, AST and ALP in comparison to that of the control. 4. The concentrations of free fatty acid and phospholipid in serum of groups B(45.7, 154.4mg/dl), C(50.0, 167.2mg/dl), D(52.5, 148.4mg/dl) and E(41.1, 159.2mg/dl) were higher(p < 0.01) in comparison to that of the control(35.2, 125.3mg/dl). And the concentrations of the carbonyl group and malondialdehyde in serum of group D(1.82, 0.52nM/mg protein) were significantly higher(p < 0.01) in comparison to the control(1.53nM/mg protein). 5. The concentrations of carbonyl group in total homogenate, mitochondrial and microsomal fraction of group D(1.45, 0.94, 1.67nM/mg protein) were significantly higher (p < 0.01) in comparison to the control(1.16, 0.66, 1.27nM/mg protein). And the concentrations of malondialdehyde in the total homogenate, mitochondrial and microsomal fraction of group D(6.70, 6.10, 1.36nM/mg protein) were significantly higher(p < 0.01) in comparison to the control(5.17, 3.64, 0.68nM/mg protein). 6. As the analytical results of the fatty acid compositions of free fatty acid in serum, the proportions of stearic acid and arachidonic acid of groups B(16.52, 12.62%), C(17.52, 15.18%), D(19.73, 13.47%) and E(17.62, 13.28%) were significantly higher(p < 0.01) in comparison to the control(14.75, 7.88%), but the proportions of oleic acid and linoleic acid of groups B(12.97, 32.59%), C(10.88, 31.23%), D(12.37, 30.66%) and E(11.95, 32.41%) were significantly lower(p < 0.01) in comparison to the control(16.44, 35.12%). Otherwise, as the results of the fatty acid compositions of phospholipid in serum, the proportions of stearic acid and arachidonic acid of groups B(39.37, 16.39%), C(40.63, 17.83%), D(42.73, 15.39%) and E(39.16, 15.70%) were significantly higher(p < 0.01) in comparison to the control(37.74, 14.24%), but the proportions of oleic acid and linoleic acid of groups B(4.03, 14.38%), C(3.54, 12.38%), D(4.52, 11.68%) and E(4.29, 13.64%) were significantly lower(p < 0.01) in comparison to the control(5.53, 16.14%). 7. As the analytical results of the fatty acid compositions of free fatty acid in total homogenate, mitochondrial and microsomal fraction of liver, the proportions of oleic acid of groups B(7.8**, 8.73**, 6.88%) and C(6.89**, 7.75**, 6.58%) were lower(**:p < 0.01) in comparison to the control(8.67, 10.08, 7.81%), but the proportions of arachidonic acid of group C(22.62, 19.79, 23.71%) were significantly higher(p < 0.01) in comparison to the control(20.93, 18.47, 22.24%). And the proportions of palmitic acid of group D(25.95**, 26.16, 26.34**%) were significantly higher(**:p < 0.01) in comparison to the control(24.43, 25.42, 23.34%). In addition, the proportions of linoleic acid of group D(23.43, 25.02, 23.95%) were also significantly higher(p < 0.01) in comparison to the control(22.17, 23.75, 21.26%). The proportions of stearic acid of group D(19.87, 19.76**%) in mitochondrial and microsomal fraction were lower(**:p < 0.01) in comparison to the control(21.01, 24.18%), and the proportions of stearic acid of group E(16.71*, 19.65**%) in mitochondrial and microsomal fraction were significantly lower(**:p < 0.01, *:p < 0.05) in comparison to the control(21.01, 24.18%), and the proportions of linoleic acid of group E(25.04, 29.20, 26.48%) in total homogenate, mitochondria and microsome were significantly higher(p < 0.01) in comparison to the control(22.17, 23.75, 21.26%). 8. As the results of the fatty acid compositions of phospholipid in total homogenate, mitochondrial and microsomal fraction of liver, the proportions of palmitic acid of group D(17.58**, 18.78*, 18.23%**) were significantly higher(**:p < 0.01, *:p < 0.05) in comparison to the control(16.28, 17.22, 16.38%), and the proportions of stearic acid of group D(36.41, 37.23, 39.53%) were also significantly higher(p < 0.01) in comparison to the control(34.18, 34.16, 36.04%). But the proportions of oleic acid(3.41*, 3.11**, 3.12**%) and linoleic acid (18.03**, 15.79**, 14.74**%) of group D were significantly lower(**:p < 0.01, *:p < 0.05) in comparison to the control(oleic : 3.63, 3.72, 3.79%, linoleic : 20.03, 18.71, 18.48%). 9. In order to determine the oxidative damages to the protein in serum, mitochondrial and microsomal fraction of the rat liver, the patterns of the SDS-PAGE were identified, but the results of SDS-PAGE were not significantly different between the control and experimental groups.

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Prediction of Acer pictum subsp. mono Distribution using Bioclimatic Predictor Based on SSP Scenario Detailed Data (SSP 시나리오 상세화 자료 기반 생태기후지수를 활용한 고로쇠나무 분포 예측)

  • Kim, Whee-Moon;Kim, Chaeyoung;Cho, Jaepil;Hur, Jina;Song, Wonkyong
    • Ecology and Resilient Infrastructure
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    • v.9 no.3
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    • pp.163-173
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    • 2022
  • Climate change is a key factor that greatly influences changes in the biological seasons and geographical distribution of species. In the ecological field, the BioClimatic predictor (BioClim), which is most related to the physiological characteristics of organisms, is used for vulnerability assessment. However, BioClim values are not provided other than the future period climate average values for each GCM for the Shared Socio-economic Pathways (SSPs) scenario. In this study, BioClim data suitable for domestic conditions was produced using 1 km resolution SSPs scenario detailed data produced by Rural Development Administration, and based on the data, a species distribution model was applied to mainly grow in southern, Gyeongsangbuk-do, Gangwon-do and humid regions. Appropriate habitat distributions were predicted every 30 years for the base years (1981 - 2010) and future years (2011 - 2100) of the Acer pictum subsp. mono. Acer pictum subsp. mono appearance data were collected from a total of 819 points through the national natural environment survey data. In order to improve the performance of the MaxEnt model, the parameters of the model (LQH-1.5) were optimized, and 7 detailed biolicm indices and 5 topographical indices were applied to the MaxEnt model. Drainage, Annual Precipitation (Bio12), and Slope significantly contributed to the distribution of Acer pictum subsp. mono in Korea. As a result of reflecting the growth characteristics that favor moist and fertile soil, the influence of climatic factors was not significant. Accordingly, in the base year, the suitable habitat for a high level of Acer pictum subsp. mono is 3.41% of the area of Korea, and in the near future (2011 - 2040) and far future (2071 - 2100), SSP1-2.6 accounts for 0.01% and 0.02%, gradually decreasing. However, in SSP5-8.5, it was 0.01% and 0.72%, respectively, showing a tendency to decrease in the near future compared to the base year, but to gradually increase toward the far future. This study confirms the future distribution of vegetation that is more easily adapted to climate change, and has significance as a basic study that can be used for future forest restoration of climate change-adapted species.

Study on Distribution and Change of Curvature of the Anterior Corneal Surface with each Age in Emmetropia (정시안의 연령별 각막전면곡률 변화와 분포에 관한 연구)

  • Kim, Chan-Soo;Sung, A-Young
    • Journal of Korean Ophthalmic Optics Society
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    • v.9 no.2
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    • pp.211-221
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    • 2004
  • The purpose of this study is to evaluate the distribution and change of curvature of the anterior corneal surface with age in emmetropia. 504 subjects who have emmetroia with good naked vision of at least 0.6-1.0 (spherical equivalent: +0.75D- -0.75D) participated in this study. The 504 subjects into 8 groups with 10 year interval from 3-year to 83-year, and their corneal curvatures were analyzed using manual keratometry. The results are as follows. In individual analysis: First, regression analysis of corneal curvature radius with age has given an equation: Y = -0.003x + 7.796 (r = -0.26). The average corneal curvature radii was measured to be $7.68{\pm}0.25mm$ at 38.3-year and range was 6.98-8.54 mm. Second, frequency of corneal curvature radius were obtained in 36% between 7.61 and 7.80 mm, 78% between 7.41 and 8.00 mm, 96% between 7.21 and 8.20 mm, 100% between 6.98 and 8.54 mm. Third, as for the comparison of corneal curvature radius with respect to sex, The mean value of male (n = 304, mean: 37.6-year $7.72{\pm}0.24mm$, Range: 7.09-8.54 mm) is larger than that of female (n = 200, mean: 39.3-year $7.62{\pm}0.24mm$, Range: 6.98-8.42 mm) by 0.1mm (p<0.01). In groups analysis: First, regression analysis of corneal curvature radius with age has given an equation: $Y=-0.0066x^2+0.0227x+7.7282$ (r = -0.90). Second, vertical and horizontal curvature radius decreased with age (p < 0.01). Especially the decrease of horizontal curvature radius were more pronounced than the decrease of vertical (horizontal:10-70 age group: 0.38 mm decrease, vertical:10-70 age group: 0.20 mm decrease). Third, difference between steep and flat meridian (astigmatism) progressively decreased with age. (low age group:0.18 mm difference, high age group: 0.08 mm difference). Fourth, the corneal curvature radius of male was larger than female's in total groups(p < 0.01). Consequently, the change of corneal curvature radius with age progressively decreased in all conditions (mean, vertical, horizontal, male, and female) and this change was more outstanding in horizontal rather than in vertical.

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Evaluation of the Behavior and Satisfaction of College Students for Family Restaurants in Seoul.Kyunggi Area (수도권 대학생들의 패밀리 레스토랑에 대한 이용 성향과 만족도 평가)

  • Shin, Mee-Hye;Lee, Ki-Yup;Kang, Kun-Og
    • Journal of the East Asian Society of Dietary Life
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    • v.17 no.3
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    • pp.445-455
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    • 2007
  • This study surveyed university students in the Seoul area regarding their awareness of family restaurants and visiting experiences. Frequency analysis was used to study the data. Most of the repliers (92.0%) had visiting experiences and visited on weekends (72.4%), paying \$10,000{\sim}15,000$ for meals. In correlation analysis of the general characteristics and reasons for visiting, there were significant differences according to gender (p<0.01) and major study area (p<0.005). During visits the factor considered most important relative to the surroundings was food styling. The factors valued most highly when selecting a family restaurant were "good taste" (43.5%) and "good discounts" (26.3%) respectively. The favorite family restaurant was Outback Steakhouse (34.7%), and 86.4% of repliers had used a membership card, with 46.9% being satisfied with using the membership card. The conditions for using a family restaurant and satisfaction differences in each category, included: the amount of food (p<0.1) for the number of visits, taste (p<0.01), food shape and styling (p<0.05), surroundings (p<0.1), and interior (p<0.05): and for the meal fee per person, price (p<0.05) and sanitation (p<0.05), all showing significant differences as reasons for using the restaurants. In the factor analysis of nine categories, three factors: service, food, and economics were acquired, and their Cronbach's ${\alpha}$ values were 0.778, 0.671, and 0.587. Satisfaction toward food and service had a positive correlation of 0.457. Satisfaction toward food and economics increased a family restaurant's overall satisfaction score, and satisfaction toward service did not have a significant influence.

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The Clinical Study on Far Diatance Spectacles for Teen-age and Twenty-age (10대와 20대의 원용안경에 관한 임상적 연구)

  • Hong, Soo-Hak;Kim, Sang-Kun;Seong, Jeong-Sub
    • Journal of Korean Ophthalmic Optics Society
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    • v.7 no.2
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    • pp.149-154
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    • 2002
  • This study is examined the difference between binocular spherical diopter and astigmatism diopter, spherical diopter, astigmatism diopter, and axis of astigmatism by sex and age with reference to the prescription of refraction for a total of 257 persons, 134 persons (male:78, female: 56)of teen-age and 123 persons of twenty-age who visit optical shop. Spherical correction diopter is mainly distributed to 280 eyes between -0.25D and -4.00D. And in astigmatism correction, for right astigmatism, 48 eyes(49.48%) are prescribed astigmatism diopter for a range of C-0.25~C-0.50D, 29 eyes(29.89%) has C-0.75~C-1.00D, 65 eyes(67.01%) has with the rule astigmatism. For left astigmatism, 43 eyes(42.57%) are prescribed astigmatism diopter of C-0.25~C-0.50D and 37 eyes(36.63%) has C-0.75C~1.00D, 73 eyes(72.27%) are shown with the rule astigmatism. And also each 108 persons(47.16%) and 28 persons(25.00%) are shown no difference between binocular spherical correction diopter and binocular astigmatism correction diopter, 94 eyes(39.49%) of teen-age and 104 eyes(42.27%) of twenty-age, male 119 eyes(41.90%) and female 79 eyes(34.34%) need correcting astigmatism. In pupillary distance, 165 persons mostly have 59~64mm.

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