미생물학회지 (Korean Journal of Microbiology)

  • 제36권3호
  • /
  • Pages.167-172
  • /
  • 2000
  • /
  • 0440-2413(pISSN)
  • /
  • 2383-9902(eISSN)
한국미생물학회 (The Microbiological Society of Korea)
권호 표지

한국 근해에서 분리한 그람양성 세균의 화학 분류학적 및 표현형적 특성

Chemosystematic and Phenotypic Characterization of Gram-positive Bacteria from Coastal Seawater, Korea

  • 발행 : 2000.09.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

제주도의 여섯 지역과 인천 작약도 근해의 해수로부터 내염성 그람양성 세균 25균주를 분리하여 화학 분류학적 특징과 표현형적 특성을 조사하였다. 분리된 균주들은 화학 분류학적 특징에 의거하여 4개의 group으로 구분되었다. Group1은 40.~49.9 mol% 의 G+C함량과 MK-7의 menaquinome type, peptidoglycan의 주요 아미노산으로 meso-A2pm을 함유하며 Bacillus pumilus와 Bacillus licheniformis, Bacillu megaterium, bacillus subtilis로 동정되었으며, Group 2는 63.9~66.4 mol% 의 G+C 함량과 MK-8을 함유하는 Arthrobacter속 세균이었으며 , Group 3은 31.0~37.6 mol%의 G+G 함량과 MK-7을 함유하며 Staphylococcus haemolyticus, Staphylococcus saprophyticyus, Staphylococcus intermedius 로 , Group 4는 72.0 mol% 의 G+C 함량과 MK-8을 주요 quinone으로 함유하는 Micrococcus luteus로 동정되었다. 분리된 세균들 중 Bacillus 속 세균들은 68%로 우세한 분포를 나타내었다.

Twenty-five halotolerant gram-positlve bacteria were isolated from the coastal seawater 01 Cheju Island and Incheon J&yakdo Chemosystematic and phenotypic characteristics were used to iuvestigate the taxonomic position of these bacteria. According to their chemosystematic characteristics, the twenty-tive isolates were divided into 4 groups. Group 1 bacteria possesed 40.1 to 49.9 inol% m DNA G+C content, menaquinone-7 as a major quinone, and meso-Alpm as a diamino acid of peptidoglycan. Group 1 tam were identified as Bacilluspumilis, Bacillus lichenifbrrizis, Bacillus megaterium, Bncill~rsubtilis. Group 2 bacteria possessed 63.9 to 66.4 mol% and MK-8. They were all in the genus Arth~obaclm-. Group 3 bacteria possessed 31.0 to 37.6 mol% and MK-7. They were identified as Staphylococcus haeniol.viicvs, Siaph~~lococc~is sapropl~j~ticns, and Siaphylococcus intermediru.. Group 4 bacterium possessed 72.0mol% and MK-8 and was identified as ~Lficrococcus ltdtezm. Bacillus species accounted for 68% of h e total isolates.

키워드

참고문헌

  1. 산업미생물학회지 v.24 천일염으로부터 고호염균의 분리 및 동정 박형숙;정명주
  2. 한국미생물학회지 v.29 한국 염전으로부터 분리한 고도 호염성 세균의 동정 및 특성 배무;이정임
  3. 한국수산학회지 v.19 Isolation and physiological properties of marine bacteria in the eutrophic coastal waters. Envrionmental factors and marine bacterial flora in the eutrophic ciastal waters 정희동;강창근;박희열
  4. J. Bacteriol v.110 Taxonomy of aerobic marine enbacteria Baumann,L.;P.Banmann;M.Mandel;R.D.Allen
  5. Int. J. Syst. Bacteriol. v.48 Psedoalteromonas prydzensis sp. nov. a psychrotrophic halotoleran bacterium from Antarctic sea ice Bowman,J.P.
  6. Appl. Microbiol. v.27 Microbiological study of a hypersaline lake in French Somaliland Brisou.J.;D.Coutois;F.Fenis
  7. Appl. Biochem. Biotechnol. v.55 Some ecological properties of the extracellular polysaccharide produced by Volcamella eurthalina F2-7 Calvo,C.;M.R.Ferrer;F.Martnez-Chewea;V.Bejar;E.Quesada
  8. J. Appl. Bacteriol. v.51 A note on the separation of natural mixtures of bacterial ubiquinions using reverse-phase partition thin-layer chromatography and high performance liquid chromatography Collins,M.D.;D.Jones
  9. Can. J. Microbiol. v.44 Halotolerant Bacillus diversity in hypersaline environments Garabito,M.J.;M.C.Marquez;A.Ventosa
  10. Mannual of methods for general Bacteriology,ASM Gerhardt,P.;R.G.E.Murrary;R.N.Costlow;W.A.Wood;N.R.Krieg;E.W.Nester;G.B.Philips(ed.)
  11. Int. J. Syst. Bacterol v.33 Numerical taxonomy of staphylococo isolated from the marine environment Gunn,B.A.;Colwell,R.R.
  12. Int. J. Food Microbiol. v.22 Proteolytic and lipolytic activities of Micrococcus roseus, Halomonas elongata and Vibrio sp isolated from Danish bacon curing brines Horrichsen,L.L.;M.C.Montel;R.Talon
  13. Appl. Env. Microbiol. v.62 Purification and characterization of a novel antibacterial protein from the marine bacterium D2 James,S.G.;C.Holmstrom;S.Kjelleberg
  14. Genus Staphylococcu, in Bergey's manual of systematic bacteriology v.Ⅱ Kloos,W.E.;K.H.Schleifer;Sneath,P.H.;Mair(ed.),N.S.;ShapeM.E.(ed.);Holt,J.G.(ed.)
  15. Genus Micrococcus on Bergey's manaul of systematic bacteriology v.Ⅱ Kocur,M.;Sneath,P.H.(ed.);Mair,N.S.(ed.);Shape,M.E.(ed.);Holt,J.G.(ed.)
  16. The halobacteraceae, In The bacteria v.8 Kushner,D.J.;C.R.Woese(ed.);R.S.Wolfe(ed.)
  17. Halophilic bacteria Life in and out of salt Recent advances in microbial ecology Kushner,D.J.;T.Hattori(ed.);Y.Ishida(ed.);Y.Maruyama(ed.);R.Y.Morita(ed.);A.Uchida(ed.)
  18. J. Gen. Microbiol. v.133 A Taxonomic Study of Heterotrophic halophilic and non-halophilic bacteria from a solar saltern Marquez,M.C.;A.Ventosa;F.Ruiz-Berraquero
  19. Int. J. Syst. Bacteriol v.39 Taxonomic Relationship of Black-Pigmented Bacillus subulis strains and proposal for Bacillus arrophaeus sp. nov. Nakamura,L.K.
  20. J. Appl. Microbiol. v.53 Types and properties of some bacteria isolation from hypersaline soil Quesada,E.;A.Ventosa;F.Rodriguez-Valeua;A.ramos-Cormenzans
  21. In Microiology of Extreme Environments and Its potential for Biotechnology Ecological distribution and biotechnological potential of halophilic microorganism Ramos-cornenzana,A.;M.S.Da Costa;J.C.Durate(ed.);R.A.D.Williams(ed.)
  22. Biophys Acta v.72 Preparation of trasfoming DNA by phenol treatment Santo,H.;V.Miura
  23. Genus Bacillus, in Bergey's manual of systematic bacteriology v.Ⅱ Sneath,P.H.A.;Mair,N.S.(ed.)Shape,M.E.(ed.);Holt,J.G.(ed.)
  24. Appl. Microbiol. v.28 no.2 Simplified approach to identification of aerobic Actinomycetes by Thin-layer chromatography Staneck,J.L.;G.D.Roberts
  25. FEMS Mico Lett. v.25 Determination of DNA base composition by reserved-phased high-performance liquid chromatiography Tamaoka,J.;K.Komagata
  26. Appl. Microbiol v.13 Salimicoccus roseus gen. nov,sp.nov, a New Moderately Halophilic Gram-Positive Coccus. System. Ventosa,A.;M.C.Marquez;F.Ruiz-Borraquero;M.Koeur
  27. Microbiol. Mol. Biol. Rev. v.62 Biology of moderately halophilic aerobic bacteria Ventosa,A.;J.J.Nieto;A.Oren
  28. Extremophles v.2 Moderately halophilic gram-positive bacterial diversity in hypersaline environments Ventosa,A.;M.C.Mequez;M.J.Garabito;D.R.Arahal
  29. Int. J. Syst. Bacteriol. v.49 Proposal to transfer Halococcus turkmenicus, Halobacterium trapanicum JCM 9743 and strain GSL-11 to Haloterrigena urkmenca gen. nov,comg.nov. Ventosa,A.M.;C.Gutierrez;M.Kamekura;M.L.Dyall Smith
  30. Applications of biotechnology to the production recovery and use of marine polysaccharides, Biotechnol v.3 Weiner,R.M.;R.R.Cowell;R.N.Jarnan;D.C.Stein;Ch.C.Somervill;D.B.Bonar