• Journal of Embryo Transfer
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• v.11 no.2
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• pp.159-166
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• 1996

본 연구는 돼지 액상정액을 인공수정용 100ml 플라스틱 병에 보존하면서 BF5희석액과 Butschwiler 희석액 간에 보존 온도별 차이를 조사하고, BF5 희석액에서의 글리세롤 농도의 효과를 조사하여 돼지 액상정액을 좀더 장기간 사용할 수 있는 방법을 찾고자 실시하였다. 돼지 액상정액을 5$^{\circ}C$ 냉장고에 보존하면서 조사한 바에 의하면, 37$^{\circ}C$에서 0.5 및 2시간 배양후의 정자운동성은 전체 보존기간동안 BF5 희석액이 Butschwiler 희석액보다 유의하게 (P<0.05) 높게 나타났고, 정상첨체비율은 두 희석액간에 차이가 없었다. 돼지 액상정액을 15$^{\circ}C$에 보존하면서 조사한 바에 의하면, 3일부터 7일 보존시 까지 정자운동성과 정상첨체비율에 있어서 Butschwiler 희석액이 BF5 희석액보다 유의하데 높게 나타났다. BF5 희석액을 이용한 돼지 액상정액의 글리세롤 농도의 효과에 있어서는 최종 글리세롤 농도가 0, 2, 3, 및 5% 보다 1%일 때 가장 높은 정자운동성과 정상첨체비율을 나타내었다. 분만율, 복당 생존자돈수 그리고 출생시 평균 생시체중은 BF5 희석애과 Butschwiler 희석액간에 차이가 없었다. 이상의 연구 결과를 종합해 볼 때 BF5 희석액을 5$^{\circ}C$에서 Butschwiler 희석액은 15$^{\circ}C$에서 6-7일 동안 돼지 액상정액을 보존할 수 있었다.

• Proceedings of the Korea Society of Poultry Science Conference
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• 2003.11a
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• pp.107-108
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• 2003

This study was conducted to investigate the effects of dilution rate and stored temperature of semen at 5, 25 and 35$^{\circ}C$ on fertility in liquid rooster semen. At 5$^{\circ}C$ cold temperature, no significant difference were found in sperm mobilities on dilution rate(1:1, 1:3, 1.6) among treatments. Sperm mobility for the conservation of 3 hours at 25∼35$^{\circ}C$ were significantly higher for 1:3 and 1:6 dilution rate(semen：diluent) groups than for 1:1 dilution rate group(P<0.05). In Fertility results after artificial insemination with the conservation of 3 hours at 5∼25$^{\circ}C$ temperature, no significant difference were found in fertility on dilution rate among treatments. Fertilities after artificial insemination with the conservation of 3 hours at 35$^{\circ}C$ were significantly higher for 1.3 and 1:6 dilution rate(semen：diluent) groups than for 1：1 dilution rate group(P<0.05).

• Journal of Korean Society of Environmental Engineers
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• v.35 no.2
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• pp.137-143
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• 2013

This study is to develop the production method of stepwise dilution gas for the evaluation of complex odor concentration by orifice tube. The basic orifice tube for 10 and 30 times of dilution sample was made at first, and with the combination of the basic orifice tubes we can continuously manufacture the stepwise dilution sample gas for air dilution sensory test ; 10, 30, 100, 300, 1,000, 3,000 times etc. The hole size of orifice tube was 0.84 mm for 10 times of dilution sample, and was 0.34 mm for 30 times. Dilution sample gas made with the basic orifice tube have an excellent reproducibility, 2%RSD. In addition, over 90% of correlation was shown between the sample made by the orifice tube and the sample by the syringe dilution method. Because there was no concentration drift of dilution gas with changes of connected pump flow, the basic orifice tube could be mounted directly with a vacuum suction box, and could be used simply as a tool for the evaluation of odor, especially on site.

• Journal of Environmental Health Sciences
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• v.31 no.4 s.85
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• pp.322-326
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• 2005

연속반응조에서의 수소생산에 대한 수리학적체류시간(희석율, D)의 영향을 $1\%$ sucrose를 함유한 $37{\pm}1^{\circ}C$ 조건에서 조사하였다. 실험결과 수리학적체류시간(희석율)의 각각의 조건에 따라 생성된 가스중 수소성분은 $50~71\%$의 범위로 발생되었다. $H_2/CO_2$ 비율은 희석율이 증가할 때 $H_2/CO_2$ 비율도 증가하였다. 최대수소생성 수율은 희석율 0.14 l/h까지는 증가하다가 이후에는 감소하였고, 수소생성 수율은 0.81 l/g sucrose이었다. Acetate 생성 수율은 butyrate생성 수율 보다 희석율 조건변화에 민감하게 변화하였다. propionate 및 solvents는 희석율 변화에 영향을 받지 않았다 biomass 수율은 희석율이 0.2 l/h 까지는 증가하였으나, 그 이상의 조건에서는 감소하였다.

• Membrane Journal
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• v.1 no.1
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• pp.13-23
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• 1991

열유도 상분리법을 이용하여 제조되는 분리막의 구조 변화를 열역학 및 속도론적 관점에서 고찰하였다. Polypropylene과 희석제로서 n-alkanes, n-fatty acids, n,n-bis(2-hydroxyethyl) tallowamine을 model system으로 하였다. 고분자/희석제 system의 상변화의 종류에 따라 다양한 형태의 분리막구조가 얻어졌다. 분리막의 구조에 영향을 미치는 변수로서 고분자/희석제간의 interaction parameter, 희석제의 분자 크기, 용액의 조성, 냉각 조건, 희석제의 결정화 온도 등이며, 각 변수의 역할을 전자현미경을 사용하여 규명하였다. 열유동 상분리법에 의하여 제조된 분리막은 inter-spherulitic 및 intra-spherulitic pore의 이중 구조로 이루어짐을 확인하였다.

• Korean Journal of Animal Reproduction
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• v.20 no.2
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• pp.101-109
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• 1996

This study was done to find out the methods of long-term use of liquid boar semen in 100 ml plastic bottle for artificial insemination and to investigate differences between Lactose-Egg yolk and Biitschwiler diluents according to storage temperature, and effect of final glycerol concent ration in Lactose-Egg yolk diluent. Liquid boar semen diluted with Lactose-Egg yolk diluent showed significantly higher sperm motility (p<0.05) after 0.5 and 2h incubation at 37$^{\circ}C$,than Butschwiler diluent at all storage length when it was preserved in the 5$^{\circ}C$ refrigerator. The NAR acrosome in Lactose-Egg yolk diluent a after 0.5 and 2h incubation at 37$^{\circ}C$, respectively, during preservation periods was similar to that in Biitschwiler diluent. When liquid boar semen was preserved at 15$^{\circ}C$, liquid boar semen in the Butschwiler diluent showed significantly higher percentages of sperm motility and NAR acrosome from third day to seventh than that in Lactose-Egg yolk diluent. In the effect of final glycerol concentration of liquid boar semen in the Lactose-Egg yolk diluent, the final glycerol concentration of 2% showed higer percentages of sperm motility and NAR acrosome than that of 0, 1, 3, and 5%. Farrowing rate, litter size and average pig weight at birth did not differ significantly between Lactose-Egg yolk and But schwiler diluents. As a result of this study, we found out that liquid boar semen can be stored for 6-7 days at 5$^{\circ}C$ in Lactose-Egg yolk diluent and at 15$^{\circ}C$ in Butschwiler diluent.

• Proceedings of the Korean Society of Postharvest Science and Technology of Agricultural Products Conference
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• 2003.04a
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• pp.121.1-121
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• 2003

증류수, 수도수, 온천수(경산 사이판 온천) 희석액 (55배 희석; I, 4배 희석; II, 2배희석; III을 사용한 반죽의 발효와 빵의 품질에 미치는 영향을 조사하였다. 경산사이판 온천수는 총고형물이 8,765 ppm 이였으며 주요 무기질로는 Na가 2296 ppm, Ca가 287 ppm, Mg와 K가 각각 65 및 8 ppm을 함유하였다. Fe, Cu, Co, F. Zn Al, S, Mo, Se 및 Si 등이 0.002~5.2 ppm을 함유하였다. 온천수를 사용한 반죽의 pH는 6.95~7.68로 증류수의 6.40보다 높았다. 반죽부피는 온천수의 농도가 높아짐에 따라 감소하였다. pH를 5.5로 조정한 온천수를 사용한 반죽의 부피는 대조구 보다 오히려 높았다. 온천수를 사용한 빵의 경도 (hardness)와 신장성(strength)는 대조구 보다 높았으나 pH를 조정한 온천수의 경우는 낮았다. 점착성은 경도 및 신장성과는 역으로 희석 온천수에서 낮았다. pH 조정 없이 구운 빵의 부드러운 정도는 4배로 희석한 온천수가 증류수 및 55배, 2배 희석 온천수보다 높았다. 쫄깃한 맛도 온천수가 대조구에 비하여 높았으며 종합적인 기호도는 4배 희석 온천수가 가장 좋았다.

• Korean Journal of Animal Reproduction
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• v.23 no.3
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• pp.257-262
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• 1999

This study was carried out to evaluate the effect of pH and storage temperature on the viability of boar sperm diluted with extender KpA for swine artificial insemination. The results obtained in this experiment were summarized as follows : 1. The viability of boar sperm diluted with KpA was higher than that with BTS during storage and especially more than 60% of sperm viability in KpA was maintained through 6 days. The pH values of all extenders were kept during storage of semens following dilution. 2. The sperm diluted with acidic (pH 6.3~6.8) or alkalic (pH 7.8~8.0) KpA and stored at 4$^{\circ}C$ or 37$^{\circ}C$ were more sensitive in viability than that with neutral pH (6.8~7.3) and at 17$^{\circ}C$ storage. But pH values of all conditions were not increased rapidly. Especially acidic and alkalic diluents were more stable in pH after dilution. Conclusively, extender pH and storage temperature was the important factors for sperm viability. The KpA setting up around neutral pH was the optimal boar semen extender for maintaining liquid semen at 17$^{\circ}C$.

• Journal of the East Asian Society of Dietary Life
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• v.11 no.1
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• pp.26-32
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• 2001

독소원성 대장균(enterotoxigenic Escherichia coli, ETEC, EC81, serotype O148:H28)이 생산하는 heat labile enterotoxin(LT)를 검색해 본 결과, reversed passive latex agglutination(RPLA)법에 있어서는 2배로 희석한 용액 (50 ng)으로부터 64로 희석한 용액 (1.56 ng)에서까지 양성반응을 보였으며 polymerase chain reaction (PCR)기법에 있어서는 10 ng으로부터 1 pg희석용액에서까지 147-base pair(bp)의 LT DNA fragment가 확인되었다. Clostridium perfringens A형 (NCTC8238, Hobbs serotype 2)이 생산한는 장독소를 검색해 본 결과, RPLA법에 있어서는 2배로 희석한 용액 (50 ng)으로부터 64로 희석한 용액 (1.56 ng)에서 까지 양성반응을 보여 독소원성대장균이 생산하는 LT와 일치하였으나, PCR기법에 있어서는 10ng로부터 10 pg 희석용액에서 까지 354-bp의 DNA fragment가 확인되어 독소원성대장균이 생산하는 LT보다 1/10의 낮은 감도를 보였다. PCR기법은 RPLA법에 비하여 훨씬 신속하고 소량의 sample로 장독소를 확인할 수 있었다.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.34 no.5
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• pp.535-539
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• 2010

We present a digital dilution chip comprising a $2{\times}6$ array of wells; the chip can be employed to change the dilution ratio as well as to initiate reactions among diluted samples by using the inter-well valve around the well. In previous dilution devices, streams comprising sample and dilution solutions were merged and separated by a branched microchannel. Therefore, the dilution ratio could not be changed until the structure of the branched microchannel was changed. The present device merges and splits the wells filled with sample or dilution solution by controlling inter-well valves around the wells. Thus, it is possible to change the dilution ratio by changing the sequence in which merging and splitting sequence of each well filled with sample or dilution solution. In experiments, we found that the chip could be used to change linear or exponential dilution ratios within an error of 16.7% and to initiate reactions among the samples within a reaction error of 17.7%.