• Title/Summary/Keyword: 흡광

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Determination of Sesame oil Adulterated with other Vegetable oils by Spectrophotometric Method (자외선 흡수특성을 이용한 참기름의 이종기름 혼입판별에 관하여)

  • 이영근
    • Journal of Food Hygiene and Safety
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    • v.8 no.3
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    • pp.151-155
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    • 1993
  • Since there have been no method which can applicable to the screening of commercial sesame oil adulterated with other vegetable oils, the present investigation was carried out particularily focusing on the the pattern of IN absorption of sesame oil and other vegetable oils. For this, a variety of oil samples prepared by the conventional method from sesame seeds, perilla seeds, com, soybean, and rice bran were analyzed by IN spectrophotometer. IN spectra of sesame oil and oil of unheated sesame seeds showed absorption peaks at 215, 230 and 290 nm. While UV spectra of com oil, perilla oil and soybean oil all showed absorption peaks at 215, 230 and 280 nm, that of rice bran oill showed peaks at 215, 290 320 nm. When sesame oil was mixed with com oil, perilla oil or soybean oil, respectively, from which the absorbance of peaks at 290 nm were lower than pure sesame oil. The peak at 320 nm which was typical absorption peak of rice bran oil was still observed in the spectnun of mixture of sesame oil with rice bran oil.

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Studies on the Stability of Natural Pigment Extracted from Ascidian shell (멍게 껍질(Ascidian shell)로부터 추출한 천연색소의 안정성에 대한 연구)

  • Park, Sin-Ho;Yang, Jae-Chan
    • Journal of the Korean Applied Science and Technology
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    • v.35 no.1
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    • pp.292-298
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    • 2018
  • In this study, Ascidian shell pigment was extracted, first using a 100.0 % ethanol solvent, proceeding with the dilution of it with DMSO (Dimethyl sulfoxide). The extracted pigment was evaluated to verify the stability. The absorbance of light have been evaluated according to pH levels and using the color-difference meter. As a result, it could be seen that absorbance and chromaticity ${\pm}a$ values were most stable at a pH level of 7.0 By keeping the sample at a pH level of 3.0, it could be observed that the absorbance and the chromaticity ${\pm}a$ values were decreased. Based on this observation, it can be deduced that the discoloration of the pigment can be prevented if kept at a neutral pH level. When antioxidants were added, the absorbance of the pigment increased, and the best effects could be seen in the ${\alpha}-tocopherol$ and glutathione samples.

Dye-binding Capacities of Proteins using Coomassie blue G250 in an Acidic Solution (단백질과 색소(色素) Comassie blue G250과의 결합능력)

  • Park, Ro-Dong;Lee, Yeon;Shin, Yong-Kwang
    • Applied Biological Chemistry
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    • v.25 no.4
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    • pp.248-251
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    • 1982
  • Commassie blue G250 produced metachromatic effect with some solvents. The absorptivity and molar absorptivity of the dye in ethanol were 82.4 and $70.4{\times}10^3$ at maximum absorption wavelength 610nm, respectively. The dye had a red from$({\lambda}_m=465nm)$ in ethanol-phosphoric acid-water solution and converted to a blue form$({\lambda}_m=590nm)$ after binding to protein. Absorbance at 590nm gave linear responses with respect to protein contents. The dye-binding capacities of proteins varied considerably with the content and source of proteins. Under the experimental condition the dye-binding capacities of bovine serum albumin, cytochrome C and ${\gamma}-globulin$ were 110, 103, and $88{\mu}g$ commassie blue G250 bound per $100{\mu}g$ protein, respectively.

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Analysis on optical property in the South Sea of Korea by using Satellite Image : Study of Case on red tide occurrence in August 2013 (위성영상을 활용한 한국 남해의 광학적 특성 연구 : 2013년 8월 발생한 적조 사례를 중심으로)

  • Bak, Su-Ho;Yoon, Hong-Joo
    • The Journal of the Korea institute of electronic communication sciences
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    • v.11 no.7
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    • pp.723-728
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    • 2016
  • This study is analyzed the optical property of red tide pixel by using Landsat-7 ETM+, Landsat-8 OLI and COMS/GOCI image. In order to sample red tide pixel, Landsat-7, 8 true color image were used and obtained coordinate of red tide pixel in the true color image. Normalized water leaving radiance(nLw) and absorption coefficient were obtained from GOCI image in the same coordinate of the true color image. When red tide was not occurred the main absorption range was 412nm and 660nm but when red tide occurred it was 660nm and absorption coefficient in 412nm are drastically reduced. It made no difference of nLw spectrum between red tide pixel and non red tide pixel in nLw, but the absolute value of nLw was low than non red tide pixel, especially 660nm and 680nm wavelength sharply decrease.

A Study on Preventive Diagnosis of the Pole Transformer by Gas Analysis -Measurement of Aging of Insulating Oil by UV-Visible Spectrophotometric Method- (개스분석에 의한 주상변압기의 예방진단에 관한 연구 -자외-가시선 흡수분광광도법에 의한 절연유의 열화도 측정-)

  • 곽희로;남영우;윤영자;남궁미옥;이동준
    • The Proceedings of the Korean Institute of Illuminating and Electrical Installation Engineers
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    • v.10 no.5
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    • pp.94-100
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    • 1996
  • Criteria for insulating oil condition of pole transformer were suggested by applying the UV-Visible spectrophotometric method. Aging of insulating oil caused the change in absorbances of oil as well as the deterioration of electrical properties of oil. By comparing the electrical properties such as tan$\delta$ and dielectric breakdown voltage with the UV-Visible absorbances of oil, following criteria for absorbance values determining oil condition were established, fair condition is below 0.580, need attention is 0.580-0.900 and poor condition is above 0.900. This W-Visible spectrophotometric method may be applied in aging analysis of oil and expected to replace the current visual method. It is necessary to develop cheap, light and portable UV-Visible spectrophotometer in near future for field application.

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A New Method for Determining the Absorption Coefficient of Oxy- and Deoxyhemoglobin by use of a Thin-fi im Optical Waveguide Sensor (박막광도파로 센서를 이용한 산화 및 환원 혈색소의 새로운 흡광계수 측정법)

  • 강신원
    • Journal of Biomedical Engineering Research
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    • v.16 no.4
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    • pp.387-394
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    • 1995
  • A simple method for determining the absorption coefficient of oxyhemoglobin and deoxyhemoglobin in human blood is proposed as an application of the complex propagation constant of a guided wave in a thin-film optical waveguide. A serial multichannel sample chamber is constructed on the waveguide to vary the interaction length between the evanescent field and the sample, and the dependence of the sensor response on the interaction length is investigated for the various concentration of two hemoglobins. The sensor response is linearly proportional to the interaction length and the concentration of two hemoglobins. The attenuation constant due to the evanescent field absorption between the samples is experimentally obtained with the designed sensor, and then the absorption coefficient is determined by the proposed method. The absorption coefficients determined by the proposed method fairly well coincided with those obtained by the conventional transmission measurement.

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Effect of Maesil (Prunus mume) Juice on the Alcohol Metabolizing Enzyme Activities (매실즙이 알코올대사 효소활성에 미치는 영향)

  • Hwang, Ja-Young;Ham, Jae-Woong;Nam, Sung-Hee
    • Korean Journal of Food Science and Technology
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    • v.36 no.2
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    • pp.329-332
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    • 2004
  • Changes in activities of alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase (ALDH) in vitro were examined by measuring maximum absorbances of ADH and ALDH at 340 nm to determine influence of Maesil (Prunus mume) on alcohol metabolism. Facilitating rates of ADH activity were 137.92, 131.58, 152,96, 218.70, 111.76, and 144.27% in Maesil juice, 5, 10, and 15% GMT, and 0.5 and 1.0% aspartic acid, respectively, ALDH activity increased in the order of Maesil juice > ALDH > GMT > aspartic acid, and facilitating rate of ALDH activity in Maesil juice was the highest at 976.44%. These results indicate alcohol metabolizing activity can be enhanced by Maesil juice.

Synthesis and Photoluminescence Properties of $BaSiO_3:RE^{3+}$ (RE=Eu, Sm) Phosphor Powers

  • Jo, Sin-Ho;Choe, Dong-Hwa
    • Proceedings of the Korean Vacuum Society Conference
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    • 2013.08a
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    • pp.180-180
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    • 2013
  • 희토류 이온이 치환 고용된 실리케이트계 형광체는 자외선으로 여기될 때 높은 발광 효율을 나타내기 때문에 광전 소자, 레이저, 형광램프에 응용할 수 있는 발광 재료로 상당한 관심이 집중되고 있다. 본 연구에서는 고상반응법을 사용하여 초기 물질 (99.99% 순도), (99.99%), (99.9%), (99.9%)을 화학 정량으로 준비하여 활성제 이온 Eu3+와 Sm3+의 함량비를 0, 0.01, 0.05, 0.10, 0.20 mol로 변화시켜 BaSiO3:RE3+ (RE=Eu, Sm) 형광체를 제조하여 그것의 발광과 흡광 특성을 조사하였다. Eu3+ 이온이 도핑된 BaSiO3 형광체의 경우에, 발광 스펙트럼은 모든 시료에서 전이에 의한 발광 스펙트럼을 보였으며, 특히 j=2에서 가장 강한 적색 형광이 피크 620 nm에서 관측되었다. 상대적으로 발광 세기가 약한 595 nm 에 정점을 갖는 주황색 발광과 705 nm 에 피크를 갖는 적색 발광 스펙트럼이 관측되었다. Eu3+ 이온의 함량비가 0.15 mol 일 때 세 영역의 발광 스펙트럼의 세기는 최대값을 나타내었다. 주흡광 스펙트럼은 Eu3+ 이온의 함량비에 관계없이 397 nm에서 관측되었다. Sm3+ 이온이 도핑된 BaSiO3 형광체의 경우에, 모든 시료는 Sm3+ 이온의 함량비에 관계없이 전이에 의한 발광 스펙트럼을 보여주고 있으며, 특히 j=7/2에서 가장 강한 주황색 형광이 피크 603 nm에서 관측되었다. 상대적으로 발광 세기가 약한 567 nm에 정점을 갖는 황색 발광과 651 nm에 피크를 갖는 적색 발광 스펙트럼이 관측되었다. Sm3+ 이온의 함량비가 0.05 mol 일 때 세 종류의 발광 스펙트럼의 세기는 최대값을 나타내었다. Sm3+ 이온의 함량비가 더욱 증가함에 따라 모든 발광 스펙트럼의 세기는 순차적으로 감소하였는데, 이 현상은 농도 소광 현상에 기인함을 알 수 있었다. 주 흡광 스펙트럼은 Sm3+ 이온의 함량비에 관계없이 406 nm에서 관측되었으며, 이밖에도 상대적으로 세기가 약한 흡광 스펙트럼이 237 nm, 377 nm와 476 nm에서 관측되었다.

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Antibacterial effect of natural extracts on oral microorganisms (천연추출물이 구강미생물에 미치는 항균 효과)

  • Hwang, Hye-Jeong;Kim, Min-Young;Kang, Kyung-hee
    • Journal of the Korea Convergence Society
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    • v.13 no.5
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    • pp.97-102
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    • 2022
  • In this study, the antibacterial effect of silkworm extract and Momordica charantia extract on S. mutans and F. nucleatum was investigated. S. mutans or F. nucleatum and 0%, 2%, 4%, 8% concentration of silkworm powder or Momordica charantia powder extract were added to the BHI liquid medium and cultured, and the sample was measured at an optical density of 600nm. As a result, S. mutans, the absorbance value was significantly decreased only in 8% of the silkworm extract, and the absorbance value was significantly decreased in all concentrations in the Momordica charantia extract. The absorbance of F. nucleatum was significantly decreased in a concentration-dependent manner in both the silkworm extract and the Momordica charantia extract. This suggests the possibility that silkworm extract and Momordica charantia extract can be used as materials for the prevention and treatment of oral diseases.

FACTORS AFFECTING THE FORMATION OF SOLUBLE GLUCAN BY LACTOCOCCUS LACTIS 1370 (유산구균 1370의 수용성 글루캔 형성에 영향을 미치는 인자)

  • Oh, In-Gyun;Yang, Kyu-Ho;Chung, Jin;Oh, Jong-Suk
    • Journal of the korean academy of Pediatric Dentistry
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    • v.27 no.2
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    • pp.185-191
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    • 2000
  • The water-soluble glucan produced by Lactococcus lactis 1370 affects the formation of dental plaque by Streptococcus mutans. In this study the factors affecting the formation of water-soluble glucan were assessed as the optical density of culture supernatant of Lactococcus lactis 1370 in the spectrophotometer. 1. The optical density of culture supernatant was high when Lactococcus lactis 1370 was cultured in M17 broth with 5% sucrose, while being low in culture supernatant of Streptococcus mutans. 2. The optical density of culture supernatant was higher when Lactococcus lactis 1370 was cultured in M17 broth with 10% sucrose than when being cultured without sucrose (p<0.05), and was higher at pH 7 than pH 5 (p<0.05). 3. The optical density of culture supernatant was the highest at $37^{\circ}C$ among $32^{\circ}C,\;37^{\circ}C\;and\;42^{\circ}C$, and was higher in the anaerobic incubator than in the aerobic incubator (p<0.05). 4. The optical density of culture supernatant was the highest in the media containing 1.0mM $CaCl_2$ (p<0.05), 2.5mM KCl (p<0.05), and 1.6mM $MgCl_2$. 5. When Streptococcus mutans was cultured in the media containing a quarter culture supernatant of Lactococcus lactis 1370 grown in M17 broth, the mean weight of produced artificial plaque was 103.0mg on the wire, whereas being significantly reduced to 5.6mg in the media containing a quarter culture supernatant of Lactococcus lactis 1370 grown in M17 broth containing 5% sucrose (p<0.05). These results indicate that the water-soluble glucan is more formed by Lactococcus lactis 1370 in the media containing sucrose or under the adequate conditions for the growth of bacteria, and inhibits the formation of artificial plaque by Streptococcus mutans.

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