• Korean Journal of Breeding Science
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• v.43 no.4
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• pp.233-240
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• 2011

Food security has been a main global issue due to climate changes and growing world population expected to 9 billion by 2050. While biodiversity is becoming more highlight, breeders are confronting shortage of various genetic materials needed for new variety to tackle food shortage challenge. Though biotechnology is still under debate on potential risk to human and environment, it is considered as one of alternative tools to address food supply issue for its potential to create a number of variations in genetic resource. The new technology, phenomics, is developing to improve efficiency of crop improvement. Phenomics is concerned with the measurement of phenomes which are the physical, morphological, physiological and/or biochemical traits of organisms as they change in response to genetic mutation and environmental influences. It can be served to provide better understanding of phenotypes at whole plant. For last decades, high-throughput screening (HTS) systems have been developed to measure phenomes, rapidly and quantitatively. Imaging technology such as thermal and chlorophyll fluorescence imaging systems is an area of HTS which has been used in agriculture. In this article, we review the current statues of high-throughput screening system in phenomics and its application for crop improvement.

• Food Engineering Progress
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• v.15 no.2
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• pp.130-135
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• 2011

Acanthopanax sessiliflorus (A. sessiliflorus) has been known as a traditional medicine having anti-stress, antioxidative and platelet aggregation inhibitory effects. This study was undertaken to investigate the functional properties of water extracts from four parts of A. sessiliflorus. Root, stem, leaf and fruit extracts from A. sessiliflorus were prepared with hot water ($80^{\circ}C$). The contents of functional substances, eleutheroside B and E, polyphenol, antioxidative activity, nitrite scavenging ability and anti-cancer activity of the extracts were determined. The contents of eleutheroside E in stem, root and fruit extracts were 542.50 ${\mu}$g/g, 343.35 ${\mu}$g/g and 30.78 ${\mu}$g/g, respectively. A large part of eleutheroside B was found in fruit (372.01 ${\mu}$g/g) and root (289.33 ${\mu}$g/g) extracts. Root and stem extracts contained 227.21 mg/100g and 131.22 mg/100g of polyphenols, respectively. Antioxidative activities (electron donating ability) of stem and root extracts were 79.87% and 77.27%, respectively. It appears that the antioxidative activities were related to polyphenol contents of the extracts. Most extracts showed 76-81.5% of nitrite scavenging ability at pH 1.2. It reveals that water extract from parts of A. sessiliflorus can inhibit formation of nitrosoamine in food. Effects of the extracts on the growth of normal and cancer cell lines were investigated. Extracts showed no cytotoxicity to normal dendritic cell line (DC2.4). Especially, the root extract promoted the growth of normal cell line. Root and stem extracts had 20-23% of inhibitory effect against stomach cancer cell line (SNU-719) and liver cancer cell line (Hep3B). These result indicated that the extracts from A. sessiliflorus can be used as functional food materials with antioxidative activity and nitrite scavenging ability to eliminate nitrosoamine in food.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.20 no.8
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• pp.303-312
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• 2019

Oxidative stress in kidneys can precede the development of chronic renal injury. We investigated the antioxidative effect of membrane-free stem cell extract (MFSCE) from adipose tissue in LLC-$PK_1$ renal proximal tubule cells. Treatment of LLC-$PK_1$ cells with MFSCE showed the up-regulation of heme-oxygenase-1, thioredoxin reductase 1, and NADPH quinine oxidoreductase-1 protein expressions, which are proteins related with antioxidative activities. When oxidative stress was induced by 3-morpholinosydnonimine (SIN-1), cell viability was decreased, indicating that LLC-$PK_1$ cells were damaged by SIN-1. However, MFSCE significantly elevated cell viability from 58.84% to 64.43% at the concentration of $2.5{\mu}g/mL$ in oxidative stress-induced LLC-$PK_1$ cells. Furthermore, MFSCE ameliorated inflammation and apoptosis in SIN-1-treated LLC-$PK_1$ cells by modulating protein expressions. Inducible nitric oxide synthase and cyclooxygenase-2 protein expressions were down-regulated when LLC-$PK_1$ cells were treated with MFSCE. Apoptosis-related proteins, including B-cell lymphoma-2-associated X protein/B-cell lymphoma-2 ratio, cleaved caspase-3, and cleaved-poly (ADP-ribose) polymerase, were also down-regulated. It indicated that MFSCE protected apoptosis against oxidative stress in LLC-$PK_1$ cells. Taken together, these results suggested that MFSCE had a protective effect against SIN-1-induced oxidative stress in LLC-$PK_1$ cells. Therefore, MFSCE could be a promising therapeutic agent for oxidative stress-induced renal injury.

• Korean Journal of Community Nutrition
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• v.24 no.3
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• pp.232-244
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• 2019

Objectives: This study evaluated the nutritional status and prevalence of metabolic syndrome of the people who participated in the KNHANES according to the number of household members. They were assessed by using information from the 2013~2014 Korea National Health and Nutrition Examination Survey (KNHANES). Methods: A total of 6,088 persons aged 19 years and over participated in 2013~2014 KNHANES, and they were classified into three groups according to the number of household members (single-person, two-person, three-person & over). The dietary behavior, nutritional status, health-related factors and the prevalence of metabolic syndrome of the subjects were investigated with using information from the survey questionnaires of KNHANES. The nutrient intake data of the subjects were obtained by the 24-hour recall method and this was analyzed for evaluating the nutrition adequacy ratio and the index of nutritional quality. The prevalence of metabolic syndrome among the subjects, and according to the study groups, was estimated using the blood and physical measurement data of the subjects. Results: As for EQ-5D index available for all the health states generated by the EQ-5D descriptive system, the single-person household member was the lowest among all the household types. The index of nutrition quality for protein, crude fiber, calcium, phosphorus, potassium, riboflavin and vitamin C in the single-person household was lower than that of the two-person or the three-person and over households (p<0.001). The mean adequacy ratio of single-person households was significantly decreased compared with that of the other types of households (p<0.001). The prevalence of metabolic syndrome was higher in the single-person households than that in the multiple-person households (p<0.001). Conclusions: These results showed that dietary behaviors, nutrition status and health status might be influenced by the number of household members. The results from this study would be useful for improving Korean people's dietary life and health status by implementing evidence-based, specialized intervention for the members of diverse types of households.

• Journal of the Korean Applied Science and Technology
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• v.36 no.2
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• pp.656-667
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• 2019

This study is a descriptive research to grasp the effects of job stress on turnover intention and to confirm the mediating effect of organizational commitment according to the extent of job stress, organizational commitment and turnover intention in the relations between job stress and Turnover Intention of nurses working in general hospitals. Method: The subjects of this study were 199 nurses are working in general hospitals, that have more than 200 beds and less than 400 beds, located in Gwangju. I surveyed them using a structured questionnaire for collecting data from Sep. 01, 2017 to Sep. 20, 2017. The collected data were analyzed by the frequency, the percentage, t-test, ANOVA, Scheffe's Test, Pearson's Correlation Coefficient, Multiple Regression Analysis and Sobel Results: In the first step, job stress as an independent variable had a statistically significant effect on organizational commitment(${\beta}=-.321$, p<.001). In the second step, job stress, an independent variable, also had an important effect on turnover intention as a dependent variable(${\beta}=.389$, p<.001). Job stress and organizational commitment were meaningful predictor variables of turnover intention in the third step. The explanatory power of two variables was 45.5%. The value ${\beta}$ of job stress in the third step was .203(p<.001) which was smaller than its value ${\beta}$,.389(p<.001), in the second step. That meant organizational commitment had the mediating effect on turnover intention. The Sobel Test was conducted to verify the significance of the extent of the mediating effects of organizational commitment. The test result was that the value Z was -3.694 and the mediating effect of organizational commitment was significant on the relation between job stress and turnover intention(p<.002). Conclusion: this study is expected be useful to find ways to reduce subjects' turnover intention by decreasing their job stress, increasing their organizational commitment and developing intervention programs as basic data.

• Korean Journal of Heritage: History & Science
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• v.52 no.2
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• pp.62-79
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• 2019

This study examined the background of Gyeongju Seochulji Pond (world heritage, historic site No. 138), a historic pond in Sam-guk-yu-sa (三國遺事), and its landscaping period when it served as the garden of the Pungcheon Lim clan (豊川 任氏) in the middle of the Joseon dynasty. For this study, a literature review of poetry, prose, and a personal anthology, and a field survey were conducted. Changes in the landscape were analyzed by comparing the landscape appearing in the literature of the Joseon period with past photographs. The results were as follows: First, even though the function and landscape at that time cannot be guessed as the objective ground from Silla to the early part of the Joseon dynasty is insufficient, it has been managed as a Byeolseo (別墅) garden as Pungcheon Lim's family resided in the area of Eastern-Namsan Mountain during the Joseon dynasty. At that time, Seochulji Pond was recognized as a historic place. It functioned as the garden of Pungcheon Lim's family as Lim Jeok (任勣, 1612~1672) built the Yiyodang pavilion (二樂堂). Second, in the literature, the Yiyodang pavilion has been called Gaekdang (客堂), Jeongsa (精舍), Byeolgak (別閣) and Byeolseo, etc. It can be seen as Nu and Jeong (樓亭), utilized for various uses. Because of this, the name Bingheoru Pavilion (憑虛樓) has mostly been in common use. Third, Seochulji Pond was positioned where the scenery is beautiful, with Gyeongju Mt. Namsan (Mt. Geumo) in the background and with a wide field and the Namcheon River flowing in the front. This was typical of Byeolseo gardens of the Joseon dynasty, combining human environments with natural environments. Fourth, the relationship with the Byeolseo garden disappeared as the head of Pungcheon Lim's family added a temple, lotus flowers, pine trees, and a bamboo forest as described in the old poetry and prose. Currently, the landscape does not appear to be significantly different from that as development has not occurred in the area of Seochulji Pond. Also, crape myrtle (Lagerstroemia indica), which now symbolizes the Seochulji Pond, was not identified in the old poetry or past photographs and is not old enough to confirm whether it was prominent at the time. Through this study, it is necessary to reconsider the spatial meanings of the gardens of the Joseon dynasty period and not to highlight the area of Seochulji Pond as a place in the legend. This is a cultural asset in the area of Eastern-Namsan Mountain and has an important meaning in terms of garden history.

• Journal of Nutrition and Health
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• v.52 no.3
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• pp.285-296
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• 2019

Purpose: This study was conducted to identify the factors affecting vegetable preferences of children based on the social cognitive theory to reduce imbalances in vegetable consumption. Methods: The survey investigated 177 elementary school students in Yangsan, Gyeongsangnam-do, in June of 2018. The subjects consisted of 44 fifth graders (27.7%) and 128 (72.3%) sixth graders. Results: Among personal factors of the Social Cognitive Theory, positive outcome expectation and self-efficacy of the vegetable preference group were significantly higher than those of the non-preference group. Negative barrier scores of the non-preference group were significantly higher than those of the preference group, and the biggest barrier was that vegetables were tasteless. Among behavioral factors, the nutritional knowledge of vegetables was high, but the degree of practice was low. Practice score of the vegetable preference group was significantly higher than that of the non-preference group. Among environmental factors, the vegetable preference group was more likely to accept advice from people around them than the non-preference group and the most influential people were doctors and parents. In the vegetable intake environment, children in the vegetable preference group had high accessibility to vegetables. Correlation analysis and regression analysis of the social cognitive factors and vegetable preferences revealed all factors except nutritional knowledge showed significant correlation with vegetable preference. And surrounding people (p < 0.01), practice (p < 0.01), and self-efficacy (p < 0.05) had positive effects on vegetable preference. Conclusion: These results suggest that providing the health benefits from eating vegetables and educating children for improving their self-confidence are necessary for increasing the preference for vegetables and their intake by children.

• Journal of Nutrition and Health
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• v.52 no.3
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• pp.297-309
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• 2019

Purpose: This study examined the relationship among the use of food-related content (FRC), dietary behaviors, and dietary self-efficacy to demonstrate the need for nutrition education to help adolescents build healthy eating habits and provide evidence for developing nutrition education programs for adolescents. Methods: Three hundred and eighty-one high school students in Seoul and Gyeonggi areas participated in the study. The subjects were divided into three groups (low, medium, and high) according to the level of use of the FRC, and their general characteristics, dietary behaviors, and dietary self-efficacy were analyzed. Correlation analysis was performed between FRC usage, dietary behaviors, and dietary self-efficacy, and the mediating effects of dietary self-efficacy on the relationship between the level of the use of FRC and dietary behaviors were estimated. Results: A higher level of FRC usage was associated with an increased daily cost of eating out and snacking, but no difference was observed in the BMI range. The subjects in a group with a high level of FRC usage ate convenience store or instant foods instead of homemade meals (p = 0.033), had a late-night meal or snack (p = 0.024), and turned to emotional eating under stress (p < 0.001) more than those in the low level group. In addition, the high level group checked the nutrition facts label more carefully when purchasing processed foods (p = 0.016) and exercised at least 30 minutes daily, not considering physical education classes (p = 0.057). The higher level of FRC use, the lower the dietary self-efficacy, whereby the subscales 'environmental stimulus control efficacy' and 'affective factor control efficacy' showed complete mediating effects. Conclusion: Given that FRC has been increased recently, adolescents are in need of support to help them control and enhance their dietary self-efficacy as well as develop healthy dietary behaviors through proper nutrition education programs.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.64 no.2
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• pp.144-151
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• 2019

The establishment rate of foxtail millet seeds is greatly affected by soil environment conditions. To enhance germination viability and stable production of foxtail millet seeds, it is important to select seeds with a high density. Therefore, this study tested the selection of high-quality seeds using salt solution (specific gravity: 1.000, 1.005, 1.010, 1.015, 1.020, 1.025, 1.030, 1.035, and $1.040g\;L^{-1}$) and investigated their germination rates, percentages of emergence, and seeding quality. In this study, three varieties were tested: 'Hwanggeum', 'Samdachal', and 'Kyeongkwan1'. The thousand seed weight of all three varieties increased proportionally with specific gravity. The highest thousand seed weight was observed at the selection with $1.040g\;L^{-1}$ specific gravity, which also had the highest germination rate of 88.3%, 86.7%, and 90.6%, for 'Hwanggeum'; 'Samdachal'; and 'Kyeongkwan1', respectively. The results of seeding quality indicated that higher the specific gravity during selection, the higher were the values of plant growth (plant height, leaf length, stem diameter, root length, root weight, and stem weight). All the three varieties were found to be longer or heavier for seeds selected at the specific gravity of $1.040g\;L^{-1}$. The packing germination viability investigation found that higher the specific gravity for selection of seeds, the higher was the percentage of emergence (PE) and the emergence rate index (ERI). The PE was the highest for seeds selected at $1.040g\;L^{-1}$ specific gravity (85.3, 83.0, and 87.0%), and ERI was also as high as $2.82d^{-1}\;m^{-1}$ or more at $1.040g\;L^{-1}$. Selection of seeds with salt solution resulted in high germination viability of foxtail millet. Therefore, sowing seeds selected at $1.040g\;L^{-1}$ specific gravity is expected to help substantially in increased productivity.

• Clean Technology
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• v.25 no.2
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• pp.129-139
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• 2019

Bamboo is an evergreen perennial plant, and it is known as one of the most productive and fastest-growing plants in the world. It grows quickly in moderate climates with only moderate water and fertilizer. Traditionally in Asia, bamboo is used for building materials, as a food source, and as versatile raw materials. Bamboo as a biomass feedstock can be transformed to prepare activated carbon using the thermal treatment of pyrolysis. The effect of process variables such as carbonization temperature, activation temperature, activation time, the amount of steam, and the mixing ratio of phosphoric acid and bamboo were systematically investigated to optimize the preparation conditions. Steam activation was proceeded after carbonization with a vapor flow rate of $0.8{\sim}1.8mL-H_2O\;g-char^{-1}\;h^{-1}$ and activation time of 1 ~ 3 h at $700{\sim}900^{\circ}C$. Carbon yield and surface area reached 2.04 ~ 20.59 wt% and $499.17{\sim}1074.04m^2\;g^{-1}$, respectively, with a steam flow rate of $1.4mL-H_2O\;g-char^{-1}\;h^{-1}$ for 2 h. Also, the carbon yield and surface area were 24.67 wt% and $1389.59m^2\;g^{-1}$, respectively, when the bamboo and phosphoric acid were mixed in a 1:1 weight ratio ($700^{\circ}C$, 2 h, $1.4mL-H_2O\;g-char^{-1}\;h^{-1}$). The adsorption of methylene blue into the bamboo activated carbon was studied based on pseudo first order and second order kinetics models. The adsorption kinetics were found to follow the pseudo second order model, which is governed by chemisorption.