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http://dx.doi.org/10.5762/KAIS.2019.20.8.303

Protective Effect of Membrane-Free Stem Cell Extract against Oxidative Stress in LLC-PK1 Cells  

Kim, Min Jeong (Department of Food Science and Nutrition & Kimchi Research Institute, Pusan National University)
Kim, Ji Hyun (Department of Food Science and Nutrition & Kimchi Research Institute, Pusan National University)
Park, Hye Sook (T-STEM Co., Ltd.)
Kim, Young Sil (T-STEM Co., Ltd.)
Cho, Eun Ju (Department of Food Science and Nutrition & Kimchi Research Institute, Pusan National University)
Publication Information
Journal of the Korea Academia-Industrial cooperation Society / v.20, no.8, 2019 , pp. 303-312 More about this Journal
Abstract
Oxidative stress in kidneys can precede the development of chronic renal injury. We investigated the antioxidative effect of membrane-free stem cell extract (MFSCE) from adipose tissue in LLC-$PK_1$ renal proximal tubule cells. Treatment of LLC-$PK_1$ cells with MFSCE showed the up-regulation of heme-oxygenase-1, thioredoxin reductase 1, and NADPH quinine oxidoreductase-1 protein expressions, which are proteins related with antioxidative activities. When oxidative stress was induced by 3-morpholinosydnonimine (SIN-1), cell viability was decreased, indicating that LLC-$PK_1$ cells were damaged by SIN-1. However, MFSCE significantly elevated cell viability from 58.84% to 64.43% at the concentration of $2.5{\mu}g/mL$ in oxidative stress-induced LLC-$PK_1$ cells. Furthermore, MFSCE ameliorated inflammation and apoptosis in SIN-1-treated LLC-$PK_1$ cells by modulating protein expressions. Inducible nitric oxide synthase and cyclooxygenase-2 protein expressions were down-regulated when LLC-$PK_1$ cells were treated with MFSCE. Apoptosis-related proteins, including B-cell lymphoma-2-associated X protein/B-cell lymphoma-2 ratio, cleaved caspase-3, and cleaved-poly (ADP-ribose) polymerase, were also down-regulated. It indicated that MFSCE protected apoptosis against oxidative stress in LLC-$PK_1$ cells. Taken together, these results suggested that MFSCE had a protective effect against SIN-1-induced oxidative stress in LLC-$PK_1$ cells. Therefore, MFSCE could be a promising therapeutic agent for oxidative stress-induced renal injury.
Keywords
Apoptosis; Inflammation; LLC-$PK_1$; Membrane-Free Stem Cell Extract; Oxidative Stress;
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