• Food Science and Preservation
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• v.22 no.1
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• pp.129-133
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• 2015

The antioxidant potentials of ten kinds of medicinal plants used as natural dyeing agents were tested. Among the plant extracts and floral waters that were examined in this study, the antioxidant activities of the DPPH and ABTS radical scavengers increased with increasing amounts of the extracts. The hot-water extracts from Aphis chinensis, Caesalpinia sappan L., Rumex crispus L., and Areca catechu effectively inhibited the DPPH and ABTS radicals at concentration below 0.1 mg/mL. The floral water obtained through steam distillation from Areca catechu, Rubia akane Nakai, Coptis chinensis, and Phellodendron amurense showed relatively valid antioxidant activities. In particular, the natural colorants extracted from Rumex crispus L., Areca catechu, and Aphis chinensis effectively suppressed the photogenerated singlet oxygen induced by a photosensitizer in in-vitro assay systems. The concentrations ($IC_{50}$) of the hot-water Aphis chinensis extract required to exert a 50% reduction effect on DPPH, ABTS, and singlet oxygen were found to be 8.5, 8.0, and $210{\mu}g/mL$, respectively. The study results suggest that extracts derived from naturally occurring colorants as well as floral waters could be used as natural antioxidants in the food and health-care industries.

• Journal of agriculture & life science
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• v.46 no.1
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• pp.141-151
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• 2012

This study was conducted to investigate the antioxidation and biological activities of 5 kinds of specialized crops (asparagus, aloe, curcumin, backnyuncho and garlic) cultivated in Namhae. All crops were freeze dried and then extracted with 10 folds of $60^{\circ}C$ hot-water, for 8 hours. Collected twice extracted water extract were also freeze dried. Dried extract samples were diluted with deionized water for examination. The total phenolics content was the highest observed in asparagus extract to $23.79{\pm}0.33mg/g$ and total flavonoids of aloe extract was the highest content $17.10{\pm}0.05mg/g$. In all samples, DPPH, ABTs and NO radical scavenging activity were depends on the concentration of the extract tended to increase. ABTs radical scavenging activity was the highest conducted $96.10{\pm}0.06%$ effect examined at $2,000{\mu}g/mL$ in asparagus hot-water extract. NO radical scavenging activity of hot-water extract from curcumin was the highest $250{\mu}g/mL$ concentration observed in $45.04{\pm}1.39%$. In all sample, tyrosinase activity was significantly inhibited with increasing of hot-water extract concentration. As a result, ${\alpha}$-glucosidase inhibition activity was the highest in hot-water extract from backnyuncho on $31.38{\pm}0.94%$ at $250{\mu}g/mL$.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2020.08a
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• pp.85-85
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• 2020

껍질째 먹는 청포도 품종인 '샤인머스켓'은 망고포도라고 불릴 정도로 단맛이 강하고 풍부한 머스켓향을 가지는 것이 특징이다. 또한 포도알이 크고 무핵처리에 의해 씨가 제거되어 소비자 만족도가 높아 최근 각광받고 있는 포도 품종이다. 샤인머스켓에 대한 국내연구는 주로 재배조건에 따른 과실특성 위주로 보고되고 있어, 본 연구는 소비가 늘고 있는 샤인머스켓의 기능성에 대한 기초자료를 제시하고자 부위별 추출조건에 따른 총폴리페놀, 총플라보노이드 함량 및 항산화 활성을 조사하였다. 샤인머스켓은 2019년 11월에 경북 상주지역에서 생산된 것으로, 과피와 과육의 2가지로 나누어 동결건조하였으며, 추출용매(증류수, 에탄올, 메탄올)와 추출용매농도(30%, 50%, 70%, 100%) 조건에 따라 시료 무게대비 10배의 용매를 가한 후 초음파방법으로 2시간 추출하였으며, 이를 2회 반복하였다. 부위별 총폴리페놀과 총플라보노이드 함량은 과육보다 과피에서 유의적으로 높게 나타났으며, 항산화 활성 또한 과피에서 월등히 높은 값을 나타냈다. 추출조건에 따른 생리활성 물질의 함량과 항산화 활성은 과육과 과피에서 다르게 나타났는데, 과육의 경우 메탄올 추출 시 활성이 높게 나타난 반면, 과피는 에탄올 추출 조건에서 활성이 더 높게 나타났다. 과육의 경우, 70% 메탄올 추출 조건에서 가장 높은 활성을 보였으며, 총폴리페놀 0.99 mg CE/g dw, 총플라보노이드 0.31 mg CE/g dw, DPPH 라디칼 소거능 2.94 mM TE/g dw, ABTS 라디칼 소거능 6.82mM TE/g dw, ORAC 11.40 mM TE/g dw, FRAP 4.92 mM Fe(II)/g dw이었다. 이와 달리 과피는 30% 에탄올 추출 조건에서의 활성이 유의적으로 높게 나타났으며, 총폴리페놀 10.23 mg CE/g dw, 총 플라보노이드 5.22 mg CE/g dw, DPPH 라디칼 소거능 44.72 mM TE/g dw, ABTS 라디칼 소거능 123.25 mM TE/g dw, ORAC 208.38 mM TE/g dw, FRAP 96.24 mM Fe(II)/g dw이었다. 이상의 결과를 볼 때, 껍질째 샤인머스켓을 섭취하는 것은 기능성 측면에서 유익하며, 추후 부위를 나누지 않은 샤인머스켓을 대상으로 추출조건 설정 및 기능성 평가에 대한 연구를 진행할 예정이다.

• Proceedings of the Korean Fiber Society Conference
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• 2002.04a
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• pp.25-28
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• 2002

비공역형 단량체인 비닐아세테이트(VAc)는 공역형 비닐계 단량체와 달리 라디칼의 활성이 너무 커서 중합 도중 빈번한 연쇄이동반응과 정지반응에 의해 분지구조의 고분자가 얻어지며 고분자량의 폴리비닐아세테이트를 얻기가 어려운 것으로 알려져 있다. 폴리비닐알코올(PVA)은 비닐알코을 단량체의 호변이성질화 때문에 단량체의 직적중합에 의해서는 얻을 수 없고 일반적으로 비닐아세테이트(VAc)를 라디칼 중합하여 얻어진 폴리비닐아세테이트(PVAc)를 비누화하여 합성한다. (중략)

• Korean Journal of Food Science and Technology
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• v.43 no.2
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• pp.200-205
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• 2011

In this study, antioxidant and anti-inflammatory effects of ethanol extracts from medicinal herb mixtures were investigated in vitro. Contents of total polyphenols in ethanol extracts from Houttuynia cordata Thunb. (HC), Carthamus tinctorius L. (CT), and MIX-2 (Houttuynia cordata Thunb., Acanthopanax gracilistylus Smith, Carthamus tinctorius L., Kalopanax pictus) were 58.98, 60.79 and 57.74 mg/g respectively. Radical scavenging activities of the ethanol extracts were examined using ${\alpha},{\alpha}$-diphenyl-${\beta}$-picrylhydrazyl radicals and 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) assay. Anti-inflammatory effects of ethanol extracts from medicinal herbs composites were examined using 5-lipoxygenase (5-LO), cyclooxygenase-2 (COX-2) and nitric oxide (NO) inhibition assays. The ethanol extracts from medicinal herb mixtures showed higher 5-LO and COX-2 inhibition activities than those from single medicinal herbs. The ethanol extracts from medicinal herb mixtures also showed strong anti-inflammatory activity through inhibition of NO production.

• Korean Journal of Medicinal Crop Science
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• v.6 no.3
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• pp.181-187
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• 1998

This study was carried out to investigate the antioxidative activities of medicinal plants. Through the examination of methanol extracts from 30 species for radical scavenging effects using DPPH method, the extracts from Rhus javanica Linne, Smilax china Linne and Polygonum cuspidatum Siebold et Zucarinii showed strong antioxidative activity. Because of its highest antioxidative activity among 30 medicinal plants, radical scavenging effects of 4 different extract compartments (n- Hexane, EtOAc, BuOH and $H_2O$ extracts) from Galla Rhois MeOH extract of Rhus javanica Linne were examined by DPPH method and antioxidant effects on the 4 different extract compartments were tested by Ferric-Thiocyanate method. Antioxidative activities of n- Hexane, EtOAc and BuOH extracts were similar or even higher than that of natural (tocopherol) or synthetic antioxidants (BHA), suggesting that major fractions for the antioxidative activity of Rhus javanica Linne were the n- Hexane, EtOAc and BuOH extract compartments.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.18 no.1
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• pp.64-80
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• 1992

Considerable interest has been generated in age-related non-enzymatic glycosylation and crosslinking of collagen in view of its extracellular nature, and its long biological half-life. The effects of UVA, which penetrates deep in dermis, and reactive oxygen species (ROS) on age- related changes of dermal collagen were studied. The amount of nonenzymatic glycosylation, fragmentation, and crosslinking of collagen were monitored from the mixtures of Type I collagen from calf skin and glucose, irradiated by UVA, with or without scavengers of ROS. At both high and low glucose dosages, non-enzymatic glycosylation was not affected by UVA irradiation. At high glucose dosage, however, glycosylation was reduced by the scavengers of superoxide radical and singlet oxygen, bolt not by hydroxyl radical scavengers. Fragmentation was increased by UVA and decreased by all ROS scavengers. Crosslinking was also enhanced by UVA, and effectively blocked crosslinking. Superoxide radical and singlet oxygen, which were produced by autoxidation of glucose independently to UVA, may encounter the initial phase of glycation. ROS generated from Amadory compounds by UVA enhanced fragmentation and crosslinking Hydroxyl radical was thought to be a major ROS affecting crosslinking. These results suggest that UVA and ROS are able to enhance age-related structural changes of collagen, as affecting many other tissue and cellular components.

• Korean Journal of Ecology and Environment
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• v.45 no.1
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• pp.11-20
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• 2012

Methanol extracts of aquatic plants were analyzed for allelopathic activities against Escherichia coli JM109 and $Microcystis$ $aeruginosa$ UTEX2385 which were compared to its 2,2-Diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activities. The radical scavenging activities were detected from the extracts of $Persicaria$ $thunbergii$, $Persicaria$ $amphibia$, $Trapa$ $japonica$, $Myriophyllum$ $spicatum$, and $Brasenia$ $schreberi$. Also, the inhibitory activities against cyanobacteria were analyzed according to the order of $B.$ $schreberi$, $T.$ $japonica$, $P.$ $amphibia$, and $M.$ $spicatum$. Most of the extracts from aquatic plants did not show any inhibition against $E.$ $coli$ except $B.$ $schreberi$. We found a positive correlation between the antioxidental activities of methanol extract of aquatic plants and the growth inhibitory activities for cyanobacteria in terms of the DPPH radical scavenging activities ($R^2$=0.381, $P$ <0.0001). The inhibitory activities of methanol extract against $E.$ $coli$ growth was not correlated with the other activities of aquatic plants ($P$ >0.04). We suggest from this study that the allelopathic effects of aquatic plants against cyanobacteria could be screened by using the bioassay based on DPPH.

• Journal of the Korean Society of Food Science and Nutrition
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• v.34 no.3
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• pp.427-432
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• 2005

This study was performed to investigate the effect of natural plant extracts on acetylcholinesterase (AChE) activity and the free radical scavenging activity. The methanolic extracts of plants were tested for AChE inhibitory activity using Ellman's colorimetric method in 96-welled microplates and antioxidant activity as the scavenging effect of 1, 1-diphenyl-2-picryl hydrazyl radical (DPPH). The results showed that AChE activities were inhibited (about 20-30%) in whole plant extract of Daucus carota var. sativa, Hypericum erectum and Fragaria yezoensis. AChE activities were inhibited (about 32-34%) in stems extract of Gingko biloba and leaves extract of Rhododendrondron yedoensa var. poukhanense. Fruit extract of Zanthoxylum schinifolium inhibited (about 18%) AChE activity. And the DPPH scavenging effects as antioxidant activity were similar to L-ascorbic acid in whole plant extract of Fragaria yezoensis and fruits extract of Comus officinalis.

• Journal of agriculture & life science
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• v.44 no.6
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• pp.133-140
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• 2010

This study was carried out to investigate the changes of ginsenoside contents and antioxidative activities from red ginseng after treated with high hydrostatic pressure (RGHHP). Crude saponin content in traditional red ginseng (TRG) and RGHHP were 21.93 and 27.29 mg/g, respectively. The contents of total phenolics, crude saponin and ginsenoside increased after treated with high hydrostatic pressure. TRG and RGHHP showed an increase 25.60% the highest content of Rb1 (14.10 mg/g and 17.71 mg/g). Also, Rg3 content compared with TRG and RGHHP increased 10.46%. The radical scavenging activity of hot water extract from red ginseng against the DPPH and ABTS radicals increased with the increasing amount of extract and RGHHP higher than TRG. The reducing power and ferric reducing antioxidant power (FRAP) assays of the red ginseng were increased in a dose dependent manner. The FRAP of TRG and RGHHP were 0.30 and 0.36 absorbance, respectively at a concentration of 10 mg/mL. The present results suggest that RGHHP would have the protective potential from oxidative stress induced by free radicals.