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A Comparison of Radical Scavenging Activity and Cyanobacteria Growth Inhibition of Aquatic Vascular Plants  

Kwon, Sung-Ho (Department of Biological Science, Ajou University)
Na, Hye-Ryun (Northeastern Asia Plant Institute)
Jung, Jong-Duk (Department of Biological Science, Ajou University)
Baek, Nam-In (Graduate School of Biotechnology & Institute of Life Science & Resources, Kyung Hee University)
Park, Sang-Kyu (Department of Biological Science, Ajou University)
Choi, Hong-Keun (Department of Biological Science, Ajou University)
Publication Information
Korean Journal of Ecology and Environment / v.45, no.1, 2012 , pp. 11-20 More about this Journal
Abstract
Methanol extracts of aquatic plants were analyzed for allelopathic activities against Escherichia coli JM109 and $Microcystis$ $aeruginosa$ UTEX2385 which were compared to its 2,2-Diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activities. The radical scavenging activities were detected from the extracts of $Persicaria$ $thunbergii$, $Persicaria$ $amphibia$, $Trapa$ $japonica$, $Myriophyllum$ $spicatum$, and $Brasenia$ $schreberi$. Also, the inhibitory activities against cyanobacteria were analyzed according to the order of $B.$ $schreberi$, $T.$ $japonica$, $P.$ $amphibia$, and $M.$ $spicatum$. Most of the extracts from aquatic plants did not show any inhibition against $E.$ $coli$ except $B.$ $schreberi$. We found a positive correlation between the antioxidental activities of methanol extract of aquatic plants and the growth inhibitory activities for cyanobacteria in terms of the DPPH radical scavenging activities ($R^2$=0.381, $P$ <0.0001). The inhibitory activities of methanol extract against $E.$ $coli$ growth was not correlated with the other activities of aquatic plants ($P$ >0.04). We suggest from this study that the allelopathic effects of aquatic plants against cyanobacteria could be screened by using the bioassay based on DPPH.
Keywords
aquatic vascular plants; cyanobacteria; DPPH; allelopathy; antioxidental activity;
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1 Basile, A., L. Ferrara, M.D. Pezzo, G. Mele, S. Sorbo, P. Bassi and D. Montesano. 2005. Antibacterial and antioxidant activities of ethanol extract from Paullinia cupana Mart. Journal of Ethnophamacology 102(3): 32-36.
2 Bushmann, P.J. and M.S. Ailstock. 2006. Antibacterial compounds in estuarine submersed aquatic plants. Journal of Experimental Marine Biology and Ecology 331: 41-50.   DOI   ScienceOn
3 Choi, J.-S., J.-H. Lee, H.-J. Park, H.-G. Kim, H.-S. Young and S.I. Mun. 1993. Screening for antioxidant activity of plants and marine algae and its active principles from Prunus davidiana. Korean Journal of Pharmacognosy 24(4): 299-303.
4 Choi, Y.-H., M.-J. Kim, H.-S. Lee, C. Hu and S.-S. Kwak. 1997a. Antioxidants in leaves of Rosa rugosa. Korean Journal of Pharmacognosy 28(4): 179-184.
5 Alves, T.M. de A., A.F. Silva, M. Brandao, T.S.M. Grandi, E. de F.A. Smânia, A.S. Junior and C.L. Zani. 2000. Biological screening of Brazilian medicinal plants. Memorias do Instituto Oswaldo Cruz, Rio de Janeiro 95(3): 367-373.   DOI   ScienceOn
6 Alzoreky, N.S. and K. Nakahara. 2003. Antibacterial activity of extracts from some edible plants commonly consumed on Asia. International Journal of Food Microbiology 80: 223-230.   DOI   ScienceOn
7 Choi, Y.-H., M.-J. Kim, H.-S. Lee, B.-S. Yun, C. Hu and S.-S. Kwak. 1997b. Antioxidative compounds in aerial partsof Potentilla fragarioides. Korean Journal of Pharmacognosy 29(2): 79-85.
8 Eaton, A.D., L.S. Clesceri, E.W. Rice and A.E. Greenberg. 2005. Standard Methods for the Examination of Water and Wastewater. 21th (ed.). 10.18-10.21.
9 Elakovich, S.D. and J.W. Wooten. 1987. An examination of the phytotoxicity of the water shield, Basenia schreberi. Journal of Chemical Ecology 13(9): 1935-1940.   DOI   ScienceOn
10 Gibson, M.T., I.M. Welch, P.R.F. Barrett and I. Ridge. 1990. Barley straw as an inhibitor of algal growth II: Laboratory studies. Journal of Applied Phycology 2(3): 241-248.   DOI   ScienceOn
11 Greca, M.D., P. Monaco, L. Previtera, G. Aliotta, G. Pinto and A. Pollio. 1989. Allelochemical activity of phenylpropanes from Acorus calamus. Phytochemistry 28(9): 2319-2321.   DOI   ScienceOn
12 Hilt, S. 2006. Allelopathic inhibition of epiphytes by submerged macrophytes. Aquatic Botany 85: 252-256.   DOI   ScienceOn
13 Gross, E.M. 2000. Seasonal and spatial dynamics of allelochemicals in the submersed macrophyte Myriophyllum spicatum. Verhandlungern der Internationalen Vereinnigung fur Limnologie 27: 2116-2119.
14 Gross, E.M., D. Erhard and E. Ivanyi. 2003. Allelopathis activity of Ceratophyllum demersum L. and Najas marina ssp. intermedia (Wolfgang) Casper. Hydrobiologia 506-509(1-3): 583-589.
15 Gross, E.M., S. Hilt, P. Lombardo and G. Mulderij. 2007. Searching for allelopathic effects of submerged macrophytes on phytoplankton-state of the art and open question. Hydrobiologia 584: 77-88.   DOI   ScienceOn
16 Jung, H.A., Y.J. Jung, N.Y. Yoon, D.M. Jeong, H.J. Bae, D.-W. Kim, D.H. Na and J.S. Choi. 2008. Inhibitory effects of Nelumbo nucifera leaves on rat lens aldose reductase, advanced glycation end products formation, and oxidative stress. Food and Chemical Toxicology 46: 3818-3826.   DOI   ScienceOn
17 Jung, S.J., D.-H. Kim, Y.-H. Hong, J.-H. Lee, H.-N. Song, Y.-D. Rho and N.-I. Baek. 2007. Flavonoids from the flower of Rhododendron yedoense var. Poukhanense and their antioxidant activity. Archives of Pharmacal Research 30(2): 146-150.   DOI   ScienceOn
18 Körner, S. and A. Nicklisch. 2002. Allelopathic growth inhibition of selected phytoplankton species by submerged macrophytes. Journal of Phycology 38: 862-871.   DOI   ScienceOn
19 Kumer, S. and K. Gopal. 1999. Screening of plant species for inhibition of bacterial population of raw water. Journal of Environmental Science and Health A34(4): 975-987.
20 Li, F.M. and H.Y. Hu. 2005. Isolation and characterization of a novel antialgal allelochemical from Phragmites communis. Applied and Environmental Microbiology 71(11): 6545-6553.   DOI   ScienceOn
21 Martin, D. and I. Ridge. 1999. The relative sensitivity of algae to decomposing barley straw. Journal of Applied Phycology 11(3): 285-291.   DOI   ScienceOn
22 Lim, B.-J., W.-H. Jheong, M.-S. Byeon and S.-O. Jun. 2000a. Inhibitory effect of Microcystis aeruginosa (Cyanophyceae) growth by in vitro. Korean Journal of Limnology 33(2): 136-144.
23 Lim, B.-J., W.-H. Jheong and S.O. Jun. 2000b. Enclosure experiments on the effects of various plant on algae. Korean Journal of Limnology 33(3): 304-310.
24 Lindström, K. 1983 Selenium as a growth factor for plankton algae in laboratory experiments and in some Swedish lakes. Hydrobiologia 101: 35-48.   DOI
25 Mathworks Inc. 2007. MATLAB-The language of technical computing. Release 7.4.0 (R2007a). Mathworks Inc. Natick. MA, USA.
26 Miller, J. 1972. Experiments in molecular genetics. Cold Spring Habor Laboratory. Cold Spring Harbor. N.Y.
27 Molish, H. 1937. Der Einfluss einer Pfanze auf die andere Allelopathie. 132pp. Fisher Jena. (English edition published in 2001).
28 Mulderij, G., W.M. Mooij, A.J.P. Smolders and E. van Donk. 2005. Allelopathic inhibition of phytoplankton by exudates from Stratiotes aloides. Aquatic Botany 82: 284-296.   DOI   ScienceOn
29 Mulderij, G., B. Mau, E. van Donk and E.M. Gross. 2007. Allelopathic activity of Stratiotes aloides on phytoplankton-towards identification of allelopathic substance. Hydrobiologia 584: 89-100.   DOI   ScienceOn
30 Muller, C.H. 1966. The role of chemical interaction (allelopathy) in vegetational composition. Bulletin of Torrey Botanical Club 93(5): 332-351.   DOI   ScienceOn
31 Nakai, S., Y. Inoue, M. Hosomi and A. Murakami. 1999. Growth inhibition of blue-green algae by allelopathic effects of macrophytes. Water Science 39(8): 47-53.   DOI   ScienceOn
32 Nam, S. and S. Park. 2007. Inibition of submerged Macrophytes on pytoplankton II. Algal growth experiments with water and plant extracts. Korean Journal of Limnology 40(4): 520-526.
33 Nakai, S., Y. Inoue, M. Hosomi and A. Murakami. 2000. Myriophyllum spicatum-releaased allelopathic polyphenols inhibiting growth of blue-green algae Microcystis aeruginosa. Water Research 34(11): 3026-3032.   DOI   ScienceOn
34 Nakai, S., Y. Inoue and M. Hosomi. 2001. Algal growth inhibition effects and inducement modes by plant-producing phenol. Water Research 35(7): 1855-1859.   DOI   ScienceOn
35 Nakai, S., S. Yamada and M. Hosomi. 2005. Anti-cyanobacterial fatty acids released from Myriophyllum spicatum. Hydrobiologia 543: 71-78.   DOI   ScienceOn
36 Nam, S., S. Joo, S. Kim, N.-I. Baek, H.-K. Choi and S. Park. 2008. Induced metabolite changes in Myriophyllum spicatum during co-existence experiment with the cyanobacterium Microcystis aeruginosa. Journal of Plant Biology 51(5): 373-378.   DOI   ScienceOn
37 Nascimento, G.G.F., J. Locatelli, P.C. Freitas and G.L. Silva. 2000. Antibacterial activity of plant extracts and phytochemicals on antibiotic-resistant bacteria. Brazilian Journal of Microbiology 31: 247-256.
38 Navarro, M.C., M.P. Monntilla, M.M. Cabo, M. Galisteo, A. Cáceres, C. Morales and I. Berger. 2003. Antibacterial, antiprotozoal and antioxidant activity of five plants used in izabal for infectious diseases. Phytotherapy Research 17: 325-329.   DOI   ScienceOn
39 Newman, J.R. and P.R.F. Barrett. 1993. Control of Microcystis aeruginosa by decomposing barley straw. Journal of Aquatic Plant Management 31: 203-206.
40 Park, M.-H., B.-H. Kim, M.-S. Han, C.-Y. Ahn, B.-D. Yoon, and H.-M. Oh. 2005. Algicidal effects of Korean oak trees against the Cyanobacterium Microcystis aeruginosa. Korean Journal of Limnology 38(4): 475-481.
41 Su, L., J.-J. Yin, D. Charles, K. Zhou, J. Moore and L. Yu, 2007. Total phenolic contents, chelating capacities. and radical-scavenging properties of black peppercorn, nutmeg, roseip, cinamon and oregano leaf. Food Chemistry 100: 990-997.   DOI   ScienceOn
42 Shan, B., Y.-Z. Cai, J.D. Brooks and H. Corke. 2007. The in vitro antibacterial activity of dietary spice and medicinal herb extract. International Journal of Food Microbiology 117: 112-119.   DOI   ScienceOn
43 Sharififar, F., M.H. Moshafi, S.H. Mansouri, M. Khodashenas and M. Khoshnoodi. 2007. In vitro evaluation of antibacterial and antioxidant activities of the essential oil and methanol extract of endemic Zataria multiflora Boiss. Food Control 18: 800-805.   DOI   ScienceOn
44 Stamp, N. 2003. Out of the quagmire of plant defense hypotheses. Quaternary Review of Biology 78(1): 23-25.   DOI   ScienceOn
45 Trouillas, P., C.-A. Calliste, D.-P. Allais, A. Simon, A. Marfak, C. Delage and J.-L. Duroux. 2003. Antioxidant, antiinflammatory and antiproliferative properties of sixteen water plant extracts used in the limousin countryside as herbal teas. Food Chemistry 80: 399-407.   DOI   ScienceOn
46 Whittaker, R.H. and P.P. Feeny. 1971. Allelochemics: Chemical interactions between species. Science 171(3973): 757-770.   DOI
47 Wong, Y.Y.P. and D.D. Kitts. 2006. Studies on the dual antioxidant and antibacterial properties of parsley (Petroselinum crispum) and cilantro (Coriandrum sativum) extracts. Food Chemistry 97: 505-515.   DOI   ScienceOn
48 Wu, Z.B., P. Deng, X.H. Wu, S. Luo and Y.N. Gao. 2007. Allelopathic effects of the surmerged macrophyte Potamogeton malaianus on Scenedesmus obiquus. Hydrobiologia 592: 465-474.   DOI   ScienceOn
49 Yeo, E.-J., K.-T. Kim, Y.S. Han, S.-Y. Nah and H.-D. Paik. 2006. Antimicrobial, anti-inflammatory, and anti-oxidative activities of Scilla scilloides (Lindl.) druce root extract. Food Science and Biotechnology 15(4): 639-642.
50 Yen, G.C. and H.Y. Chen. 1995. Antioxidant activity of various tea extracts in relation to their antimutagenicity. Journal of Agricultural and Food Chemistry 43: 27-32.   DOI   ScienceOn
51 Zhou, S., S. Nakai, M. Homomi, Y. Sezaki and M. Tominaga. 2006. Allelopathic growth inhibition of cyanobacteria by reed. Allelopathy Journal 18(2): 277-285.