Biological Activities of Specialized Crops in Namhae

남해군 특용작물의 생리활성

  • Received : 2011.07.19
  • Accepted : 2012.02.24
  • Published : 2012.02.28

Abstract

This study was conducted to investigate the antioxidation and biological activities of 5 kinds of specialized crops (asparagus, aloe, curcumin, backnyuncho and garlic) cultivated in Namhae. All crops were freeze dried and then extracted with 10 folds of $60^{\circ}C$ hot-water, for 8 hours. Collected twice extracted water extract were also freeze dried. Dried extract samples were diluted with deionized water for examination. The total phenolics content was the highest observed in asparagus extract to $23.79{\pm}0.33mg/g$ and total flavonoids of aloe extract was the highest content $17.10{\pm}0.05mg/g$. In all samples, DPPH, ABTs and NO radical scavenging activity were depends on the concentration of the extract tended to increase. ABTs radical scavenging activity was the highest conducted $96.10{\pm}0.06%$ effect examined at $2,000{\mu}g/mL$ in asparagus hot-water extract. NO radical scavenging activity of hot-water extract from curcumin was the highest $250{\mu}g/mL$ concentration observed in $45.04{\pm}1.39%$. In all sample, tyrosinase activity was significantly inhibited with increasing of hot-water extract concentration. As a result, ${\alpha}$-glucosidase inhibition activity was the highest in hot-water extract from backnyuncho on $31.38{\pm}0.94%$ at $250{\mu}g/mL$.

경남 남해군에서 재배되고 있는 특용작물인 아스파라거스, 알로에, 울금, 백년초 및 마늘을 시료로 하여 각각의 열수추출물을 제조하고, 총 페놀 화합물, 플라보노이드의 함량 및 항산화 활성을 중심으로 생리활성을 분석하였다. 총 페놀 화합물의 함량은 아스파라거스 열수추출물이 $23.79{\pm}0.33mg/100g$으로 가장 높았고, 다음으로 울금 열수추출물이 $21.56{\pm}0.66mg/100g$으로 정량되었다. 플라보노이드 함량은 알로에 열수추출물이 $17.10{\pm}0.05mg/100g$으로 가장 높은 함량이었으며, 다음으로 아스파라거스 열수추출물 ($11.68{\pm}0.83mg/100g$), 울금 열수추출물 ($11.33{\pm}0.18mg/100g$)의 순이었다. 모든 시료에서 전자공여능, ABTs 라디칼 소거능 및 NO 라디칼 소거능은 추출액의 농도에 의존적으로 그 활성이 증가하는 경향을 보였다. ABTs 라디칼 소거능은 아스파라거스 열수추출물이 가장 높아 $2,000{\mu}g/mL$ 농도에서 $96.10{\pm}0.06%$의 소거능을 나타내었으며, NO 라디칼 소거능은 $250{\mu}g/mL$ 농도에서 울금 열수추출물이 $45.04{\pm}1.39%$로 가장 뛰어난 활성을 나타내었다. 5종의 모든 시료에서 열수추출물의 농도가 증가할수록 tyrosinase 저해 활성이 유의적으로 증가하였으며, ${\alpha}$-glucosidase 저해 활성은 $250{\mu}g/mL$ 농도에서 백년초 열수추출물의 활성이 $31.38{\pm}0.94%$로 가장 높았다. 본 연구를 종합하여 볼 때 5종의 남해군 특용작물의 항산화 활성은 아스파라거스가 가장 높아 기능성 식품소재로서 활용이 기대된다.

Keywords

References

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