• Korean Journal Plant Pathology
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• v.12 no.2
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• pp.219-255
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• 1996

사과 겹무늬썩음병의 주 전염원은 가지 병반에서 형성되는 자낭포자 및 병포자이므로, 가지에서의 포자형성 또는 포자의 분산을 화학적 또는 물리적 방법으로 저해할 수만 있다면 효과적으로 병을 방제할 수 있을 것으로 생각했다. 전염원을 줄이기 위한 첫 번째 시도로 1992년 이른봄 가지에 형성된 사마귀를 호미로 제거한 결과, 병포자의 분산과 과실발병 억제효과에 있어서 무처리와 유의적인 차이가 인정되지 않았다. 두 번째 시도로 포자의 분산을 막기 위해 1993년 이른봄에 겹무늬썩음병에 걸린 가지를 비닐필름으로 감고 5월부터 9월까지 20일 간격으로 살균제를 살포한 결과, 통계적으로 유의성이 인정되는 병 방제효과가 있었고, 나무의 생육에 있어서도 콜크층이 이상 비대하는 것 이외에 별다른 이상이 없었다. 이 개념을 더욱 발전시켜 1994년 이른봄에는 병든 가지를 수용성 고분자 물질인 polyvinyl alcohol로 도포하고 수배 분산 포자의 수를 조사한 결과, 포자의 분산량이 크게 줄어져 실용화의 가능성이 시사되었다.

• Journal of the Korean Chemical Society
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• v.64 no.4
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• pp.236-238
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• 2020

• Research in Plant Disease
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• v.27 no.4
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• pp.129-136
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• 2021

Rice is responsible for the stable crop of 3 billion people worldwide, about half of Asian depends on it, and rice is grown in more than 100 countries. Rice diseases can lead to devastating economic loss by decreasing yield production, disturbing a stable food supply and demand chain. The most commonly used method to control rice disease is chemical control. However, misuse of chemical control can cause environmental pollution, residual toxicity, and the emergence of chemical-resistant pathogens, the deterioration of soil quality, and the destruction of biodiversity. In order to control rice diseases, research on alternative biocontrol is actively pursued including microorganism-oriented biocontrol agents. Microbial agents control plant disease through competition with and antibiotic effects and parasitism against plant pathogens. Microorganisms isolated from the rice rhizosphere are studied comprehensively as biocontrol agents against rice pathogens. Bacillus sp., Pseudomonas sp., and Trichoderma sp. were reported to control rice diseases, such as blast, sheath blight, bacterial leaf blight, brown spot, and bakanae diseases. Here we reviewed the microorganisms that are studied as biocontrol agents against rice diseases.

• Weed & Turfgrass Science
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• v.5 no.1
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• pp.29-34
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• 2016

Large patch disease in Zoysia japonica Steud. is the most destructive disease in turfgrass. For large patch management, it has been dependent on chemical controls but pesticides are harmful to soil, water and biodiversity. In this study, we evaluated 4 Streptomyces spp. strains (S2, S5, S8 and S12) which were selected in previous studies using metagenome approaches. Root colonization of the strains, large patch suppressing effect and the pathogen density change in actual golf course were investigated to evaluate biological control potential of the strains. All strains exhibited reliable root colonization ability that strains populations were higher than $6log\;cfu\;g^{-1}$ in turfgrass rhizosphere. The pathogen density, with S8 treatment, was detected average of 0.7 after a week and average of 1.2 after 4 weeks. Disease control and suppressive the pathogen population by S8 strain showed higher efficiency than other strains. S8 was applied in an actual golf course for the large patch control and pathogen density. The pathogen density in S8 treatment plot was detected below 1.6 per toothpick and lower compared with untreated plot. The results indicated that pathogen density was suppressed by S8 and the stain has great potential as a biological control agent for the large patch.

• Journal of Conservation Science
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• v.33 no.4
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• pp.255-266
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• 2017

Chemically derived pesticides have been used to prevent biological damage to domestic cultural property. However, their use is gradually being restricted due to the harmful effects on the human body and environment. Therefore, there is a growing interest in the search for new antifungal biopharmaceuticals whose safety has been confirmed by toxicity evaluation through animal experiments. This paper presents methods of toxicity evaluation of natural biocides using Sprague-Dawley rats and New Zealand White (NZW) rabbits. Safety of the natural biocide extract of Asarum sieboldii was evaluated using single-dose oral and dermal toxicity tests in Sprague-Dawley rats, and eye and skin irritation tests in NZW rabbits. The extract has proven antimicrobial and insecticidal activities against wood-rotting fungi and termites. After single oral administration to rats, the $LD_{50}$ values were determined to be over 4,000 and 2,000 mg/kg for males and females, respectively. After single dermal administration to rats, the $LD_{50}$ values exceeded 10,000 mg/kg for both males and females. The extract was identified to be non-irritant to the rabbit eye, and only slightly irritant to the rabbit skin. In this study, we confirmed the safety of the A sieboldii extract through animal testing. Due to the harmfulness of humidifier disinfectants, focus is on the safety of chemical pesticides, and toxicity evaluation is suggested as the basic method for hazard evaluation.

• Research in Plant Disease
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• v.16 no.1
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• pp.27-34
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• 2010

This study was performed to develop a formulation using an antagonistic bacterium Bacillus amyloliquefaciens A-2 to control tomato leaf mold caused by Fulvia fulva. B. amyloliquefaciens A-2 was grown in a medium with rice oil and mixed with various carrier and additives. One of the formulations, A2-MP, showed the best disease control value among the tested formulations. The disease control value of A2-MP at 100-fold and 500-fold diluted treatment was not significantly different from that of chemical fungicide triflumizole in a growth chamber. Although disease control effect was decreased by serial diluted treatment of the prepared A2-MP, 1,000-fold diluted treatment of A2-MP still showed high disease control value of 72.0%. For the green house experiments, the disease control values of A2-MP was indicated as 79.4% which is similar to that of chemical fungicide, triflumizole showing 79.6%. When the disease control activity of the formulation A2-MP was compared in tomato production conditions, disease control values of 100-fold diluted A2-MP and 3,000 fold diluted triflumizole exhibited 60%, 81.6%, respectively. The disease control efficiency by A-2MP was 73% of the disease control value of chemical fungicide. The formulation A-2MP maintained the stable bacterial viability and disease control activity when stored at $4^{\circ}C$. This result suggested that A-2MP develped from B. amyloliquefaciens A-2 could be used to control tomato leaf mold.

• 보존과학연구
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• s.32
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• pp.123-136
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• 2011

There are more than 2,800 different species of termites in the world, but just there is one species (Reticulitermes speratus kyushuensis Morimoto) in Korea. Once wooden structures are damaged by termites, we are applying chemical control methods such as fumigation, treatment of insecticidal and antiseptic chemicals, soil termiticide treatment, and termite colony elimination system to pest control. But in Korea, the termite infestation is gradually increasing, so it is essential to study on the present situation of termite control in US or Japan. Accordingly, in this part we have studied focusing on the case of US. In the US, there are three groups of termites : Subterranean, Drywood, Dampwood termites, and they caused more severe infestation compared with Korea. When a structure has become infested with termites, it is important that appropriate action must be taken: the chemical pest control (soil treatment, termite baiting, termite colony elimination), the modification and maintenance of buildings and the regular inspection for follow-up. And with consideration for different characteristics of termites, the process of pest control is made according to each species of termites. Most of the subterranean termite control is done by applying either termiticide to the soil or termite monitoring and baiting system around the structure. On the other hand, drywood termite control methods can be categorized as either the treatment for the whole structure or the treatment for localized area. Applications to the whole structure are done by fumigants or heat and the localized treatments are carried out with chemicals as well as heat, freezing, microwave and electricity.

• The Korean Journal of Mycology
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• v.44 no.3
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• pp.193-195
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• 2016

Korean ginseng (Panax ginseng) possesses various biological and pharmacological properties. Damping-off is a critical disease on ginseng seedlings, which is caused by the fungal pathogens Rhizoctonia solani and Pythium sp.. This disease is generally controlled by the application of fungicides, but also biological control is an efficient and environmentally friendly way to prevent ginseng damping-off. In a previous study, we screened soil-borne bacteria with potential applications as biological control agents for ginseng damping-off and selected the bacterial strain Streptomyces sp. A3265, producing antifungal substances guanidylfungin and methylguanidylfungin. In this study, we investigated control efficacy of Streptomyces sp. A3265 against ginseng damping-off in the field. As a result, the incidence of damping-off was significantly reduced when soaking ginseng seeds in the culture broth of Streptomyces sp. A3265.

• Research in Plant Disease
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• v.20 no.2
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• pp.71-78
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• 2014

Gamma irradiation and its convergence with nano-silver particles and sodium dichloroisocyanurate (NaDCC) were investigated to inhibit germination and mycelial growth of Botrytis elliptica, the pathogen of lily leaf blight. In addition, the same treatments were studied on the process of disease development with detached leaf of lily cv. Siberia. Spray inoculation, which is closer to natural infection than wound inoculation, can be a way to investigate infection ability of the treated pathogen. The irradiating dose required to reduce the population by 90%, $D_{10}$, was 526 Gy irradiating with 0-2000 Gy gamma ray on the conidial suspension as well as the growing mycelia. Even at 2000 Gy, the mycelium was not killed but just delayed its growth at 1-2 days behind. Convergent treatment with 40 mg/l of NaDCC just before 200 Gy gamma irradiation was the best way to decrease the conidial germination about 1/1000 times. The control values of gamma irradiation were 23% and 19.5% at wound inoculation and spray inoculation, respectively. On wound-inoculation, the control value of NaDCC only was 89%, and that of NaDCC convergent with 200 Gy gamma irradiation was 32%. On sprayinoculation, the highest control value was NaDCC at 50%, and that of NaDCC convergent with gamma irradiation was 24%.

• Fire Science and Engineering
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• v.14 no.3
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• pp.19-26
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• 2000

As a basic physical property of chemical substances, the flash point is of great importance as far as the prevention of chemical disasters is concerned. The measurement of flash point is not easy. and the number of c(Impounds for which systematic data have been collected pitifully small. Because, a need exists for some means of predicting the flash point of compound for which experimental data are unavailable, not only those which are currently in use or at the production stage, but also, those not compounds which are in the process of synthesis and design. The present research concerns the application of OCD (organic conception diagram) to the association between chemical structures and flash points, and an attempt made to discover a method of predicting the approximate flash point of a compound from its chemical structure alone.