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http://dx.doi.org/10.12654/JCS.2017.33.4.03

Toxicity Evaluation of Asarum Sieboldii Extract for Human's Safety  

Kim, Young Hee (Restoration Technology Division, National Research Institute of Cultural Heritage)
Jo, Chang Wook (Restoration Technology Division, National Research Institute of Cultural Heritage)
Hong, Jin Young (Restoration Technology Division, National Research Institute of Cultural Heritage)
Lee, Jeung Min (Restoration Technology Division, National Research Institute of Cultural Heritage)
Kim, Soo Ji (Restoration Technology Division, National Research Institute of Cultural Heritage)
Jeong, So Young (Restoration Technology Division, National Research Institute of Cultural Heritage)
Publication Information
Journal of Conservation Science / v.33, no.4, 2017 , pp. 255-266 More about this Journal
Abstract
Chemically derived pesticides have been used to prevent biological damage to domestic cultural property. However, their use is gradually being restricted due to the harmful effects on the human body and environment. Therefore, there is a growing interest in the search for new antifungal biopharmaceuticals whose safety has been confirmed by toxicity evaluation through animal experiments. This paper presents methods of toxicity evaluation of natural biocides using Sprague-Dawley rats and New Zealand White (NZW) rabbits. Safety of the natural biocide extract of Asarum sieboldii was evaluated using single-dose oral and dermal toxicity tests in Sprague-Dawley rats, and eye and skin irritation tests in NZW rabbits. The extract has proven antimicrobial and insecticidal activities against wood-rotting fungi and termites. After single oral administration to rats, the $LD_{50}$ values were determined to be over 4,000 and 2,000 mg/kg for males and females, respectively. After single dermal administration to rats, the $LD_{50}$ values exceeded 10,000 mg/kg for both males and females. The extract was identified to be non-irritant to the rabbit eye, and only slightly irritant to the rabbit skin. In this study, we confirmed the safety of the A sieboldii extract through animal testing. Due to the harmfulness of humidifier disinfectants, focus is on the safety of chemical pesticides, and toxicity evaluation is suggested as the basic method for hazard evaluation.
Keywords
Natural biocide; Single oral toxicity; Single dermal toxicity; Skin irritation test; Safety;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
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