• The Korean Journal of Ecology
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• 제23권3호
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• pp.255-266
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• 2000

생물권보전지역으로 지정된 점봉산의 온대낙엽수림(참나무 성숙림)에서 임상초본식물의 공간분포를 정량화하고, 이들의 공간분포에 미치는 미소환경요인의 영향을 파악하였다. 대부분의 임상초본식물들은 영구 방형구내 미소지소간에 큰 변이를 나타내었다. 초봄에 출현하는 임상초본식물중 공간의존도가 높은 식물은 현호색, 애기나리 및 노랑제비꽃이었고, 가을에 출현하는 임상초본식물중 공간의존도가 높은 식물은 벌깨덩굴, 쌀새 및 도라지모시대이었다. 초봄에 출현하는 홀아비바람꽃과 얼레지 그리고 가을에 출현하는 도라지모시대와 쌀새의 공간의존거리가 상대적으로 짧은 편이었다. 초봄에 출현하는 노랑제비꽃, 애기나리 및 얼레지는 관목이 적고, 낙엽층이 얇으며,토심이 깊고, 토양수분함량, 유기물함량 및 염기성양이온함량이 상대적으로 적은 생육지를 선호하였고, 애기앉은부채 와 현호색은 관목이 밀집하고, 낙엽층이 두꺼우며, 토심이 얇고, 유기물함량, 토양수분함량 및 영양염류함량이 높은 생육지를 선호하였다. 오리방풀은 빛조건이 좋고, 낙엽층이 두꺼우며, 토양수분함량과 영양염류가 풍부한 생육지를 선호하였고, 단풍취, 대사초, 개별꽃, 참취, 쌀새 및 도라지모시대는 토심이 깊고, 낙엽층이 얇으며, 토양수분함량과 영양염류함량이 상대적으로 적은 생육지를 선호하였다. 본 참나무 성숙림에서 토양수분함량, 유기물함량 및 영양염류함량은 바람, 미소지형 및 관목의 밀집정도에 의해 공간적으로 재배치되는 낙엽층 두께에 의해 영향을 받고 있기 때문에 초봄과 가을에 관찰되는 임상초본식물의 공간분포는 낙엽층의 불균등한 분포에 의해 가장 큰 영향을 받고 있는 것으로 추정되었다.

• 한방안이비인후피부과학회지
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• 제24권3호
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• pp.17-26
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• 2011

Objectives : Corydalis tuber has been used for promoting blood circulation and for relieving pain in Oriental medicine. In the present study, we focused on the antimicrobial activity of Corydalis tuber and compared its antimicrobial activity with the processed Corydalis tuber. Methods : Processing of Corydalis tuber was accomplished by immersing in 5% of acetic acid for 12 h and then by roasting at $250^{\circ}C$ for indicated time periods(0-30 min). Minimum inhibitory concentration(MIC) and the zone of growth inhibition were determined against Propionibacterium acnes(P. acnes). Results : The methanolic extracts of Corydalis tuber showed potent antimicrobial effect(MIC 62.5 ${\mu}g/ml$). Its alkaloidal component, dehydrocorydaline, also exhibited antibacterial activity(MIC 25.0 ${\mu}g/ml$). After processing of Corydalis tuber, its inhibitory effect on the growth of P. acnes was significantly enhanced compared with that of unprocessed Corydalis tuber. Furthermore, elevated content of dehydrocorydaline was found in the processed than the unprocessed Corydalis tuber. However, the different roasting minutes effected on antimicrobial activity. The best roasting time of Corydalis tuber was 10 min, while roasting for the time above 15 min resulted in diminishing antimicrobial activity. Thus, it was concluded that the standardized processing condition of Corydalis tuber should be established to obtain enhanced antimicrobial(P. acnes) activity. Conclusion : For antimicrobial effect against P. acnes, the best processing condition of Corydalis tuber is immersing in 5% of acetic acid for 12 h and by roasting at $250^{\circ}C$ for 10 min.

• The Korean Journal of Ecology
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• 제26권4호
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• pp.189-197
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• 2003

남한산성 지역에서 춘계단명식물인 현호색 자연집단에 대해 2년간 종자생산의 특성을 조사하였다. 개화는 4월초에, 종자생산은 5월초에 일어나 개화로부터 종자생산까지는 30일이 소요되었으며 생식기관의 수가 감소하는 시기는 낙화 및 결실기 사이가 가장 큰 것으로 나타났다. 개체 당 생산하는 꽃의 수는 1∼13개, 종자의 수는 0∼76개, 열매 당 종자의 수는 0∼20개이었다. 한 개체 내에서 열매 당 종자생산량은 가장 아래에 위치한 꽃이 11.8개로 많았으며 상부로 이행할수록 급격히 감소하였다. 동일한 개체가 생산하는 종자의 수는 매년 큰 폭으로 변하였으며 작은 개체는 다음해 많은 수의, 큰 개체는 적은 수의 증자를 생산하였다. 생육초기 개화체의 비율은 개체의 크기(괴경의 부피)가 증가할수록 증가하였으며 가장 작은 크기 계급인 <100 ㎣에서 5.0%(1999)∼5.4%(2000)를, 600㎣≤ 에서 100%를 나타냈다. 한편, 각 계급별 평균 꽃의 수는 2000년이 1999년보다 많았다. 특히 900 ㎣≤에서는 6.5개(1999)와 13.2개(2000)로 현저한 차이를 보였다. 생육후기 개화체의 비율은 <100 ㎣에서 13.3%이었고 500 ㎣≤ 에서 100%이었다. 따라서 생육초기보다 개화체의 비율이 다소 높게 나타났다. 괴경의 체적이나 건중량의 크기에 따른 열매의 수 및 종자의 수는 대체로 증가하였지만 경향성은 뚜렷하지 않았고 동일 크기계급 내에서도 개체마다 편차가 심하였다. 한편, 엽면적이 증가할수록 열매의 수나 종자의 수는 뚜렷이 증가하였다. 따라서 종자의 생산량은 생육기간동안 광합성량과 관계가 깊은 것으로 볼 수 있었다.

• 환경생물
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• 제24권4호
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• pp.359-367
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• 2006

본 연구는 불영계곡 일대의 관속식물을 조사한 후 무분별한 개발과 채취로 인해 점점 멸종되어가고 있는 희귀식물과 한국특산식물 및 식물구계학적 특정식물종을 제시함으로써, 식물자원과 식생 보전을 위한 기초 자료를 마련하고자 본 연구를 수행한바 다음과 같은 결론을 도출하였다. 본 조사지역의 관속식물상은 101과 340속 547종 80변종 12품종 1아종 1교잡종으로 총 641종류(taxa)가 확인되었으며, 이중 희귀식물에는 식재수종을 제외한 창포(보존우선순위 54번), 꼬리겨우살이 (170번), 등칡 (57번), 쥐 방울덩 굴 (151번), 태 백제 비꽃 (202번), 지리산오갈피(58번), 꼬리진달래 (140번), 개불알꽃 (29번), 미치광이풀(208번)등 총 9종류(taxa)가 확인되었다. 한국특산식물에는 키버들, 각시족도리, 흰명아주여뀌, 숲개 별꽃, 진범, 점현호색, 흰현호색, 갈퀴현호색, 꽃황새냉이, 흰털괭이눈, 터리풀, 틸조록싸리, 지리산오갈피, 새며느리밥풀, 병꽃나무, 털잔대, 고려엉겅퀴, 가야은분취 등 총 18종류(taxa)가 확인되었다. 또한, 식물구계학적 특정식물종은 V등급에 식재종인 왕벚나무, IV등급에는 측백나무, 승마, 애기기린초, 꼬리진달래 등 13종류, III등급에는 개쇠뜨기, 돌단풍, 참당귀, 고려엉겅퀴 등 17종류, II등급에는 처녀치마, 동자꽃, 참바위취, 노랑제비꽃 등 15종류, 그리고 I등급에는 일월비비추, 촛대승마, 애기괭이눈, 초롱꽃 등 35종류로 분석되어 총 81종류(12.6%)가 확인되었다. 귀화식물에는 12과 40속 51종 2변종으로 총 53종류(taxa)가 확인되었으며, 귀화율은 8.2%, 도시화지수는 20.7%로 분석되었다. 최근 들어 불영계곡 일대는 도로 확장 등의 개발계획과 무분별한 관광객의 증가로 인해 훼손이 가속화되고 있는 실정이다. 따라서 빠른 시일에 생태계복원계획에 대한 다양한 대책과 제한적인 국토개발계획의 재수립이 필요하며, 희귀식물이나 특산식물 등이 분포하고 있는 서식처를 보전지역으로 지정하여 관리하는 방안이 필요할 것으로 사료된다.

• 생약학회지
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• 제39권4호
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• pp.305-309
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• 2008

Dehydrocorydaline was isolated from the roots of Corydalis ternata (Papaveraceae) and identified by the comparison the $^1H-$ and $^{13}C$-NMR spectral data with those of authentic sample. The content of dehydrocorydaline was determined by the HPLC analysis based on extraction of ground plant material. Quantitative analysis of dehydrocorydaline in MeOH extract of C. ternata by HPLC showed $1.31{\pm}0.95%$ in 20 samples collected throughout regions of Korea.

• 생약학회지
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• 제30권1호
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• pp.54-58
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• 1999

A method using coloric and spectrophotometric detection have been developed for the identification of the tertiary or quaternary alkaloids contained in Corydalis tuber and its preparations. The principle is based on the formation or decomposition of complex compounds. The complex compound of the tertiary and quaternary alkaloids have been formed by adding tetrathiocyanatocobaltate [II] ion to the test soln. Diverse crude drugs were screened using this method and the results indicated that isoquinoline, aconitine-type alkaloids in crude drugs can be readily detected. The method is simple, convenient, reproducible and applicable to the verification of the crude drug Corydalis tuber and its preparations.

• 생약학회지
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• 제27권4호
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• pp.289-294
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• 1996

In previous paper, we described the induced callus of Corydalis remota contains a significant amount of alkaloids. This study describes an optimal condition to maximize alkaloid production. The suspension cultures maintained alkaloid production ability after fifth subculture and a small amount of alkaloid seemed to be released out of cells. The yields of alkaloid by cultured cells was varied depending on the concentrations of NAA, carbon sources and phosphate ion and depending on the vitamin combinations and concentrations. Biosynthetic precursor and an elicitor treatment also affected the total alkaloid yield of the cultures. The optimal conditions for alkaloid production were as follows: 1) MS basal salt containing 30 g/l of glucose, 1.0 mg/l of NAA, and vitamins of LS medium should be used. 2) The culture should be treated with tyrosine 20 mg/l, and yeast extract 1.5 ml/l after the culture reached a stationary phase of growth. Five alkaloids were isolated from the cultures and they were characterized. The spectral data unambiguously revealed that the isolated compounds were dihydrosanguinarine, protopine. tetrahydropalmatine, allocyptopine and ambinine, respectively.

• 생약학회지
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• 제27권2호
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• pp.91-95
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• 1996

In the course of searching for acetylcholinesterase inhibitors from crude drugs, it was found that total MeOH extract of Corydalis Tuber showed significant inhibitory effect on acetylcholinesterase. To isolate acetylcholinesterase inhibitors from Corydalis Tuber, total MeOH extract of the the crude drug was subjected to activity guided fractionation. The MeOH extract was suspended in water and fractionated with methylene chloride and subjected to acid-base fractionation. Silica gel column chromatography of the basic fraction which showed significant inhibitory effect on acetylcholinesterase was carried out and 5 subfractions (1-5) were obtained. From subtraction 4, compound I was isolated. The structure of isolated compound I was identified by spectroscopic parameters of $^1H-NMR$, $^{13}C-NMR$, EI-MS and FAB-MS. The compound I was identified as berberine. It was found from the Lineweaver-Burk plot that berberine was a reversible and specific inhibitor of acetylcholinesterase having 90% inhibitory effect at the concentration of $2.5{\mu}M$.

• 대한본초학회지
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• 제31권4호
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• pp.27-33
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• 2016

Objective : For the purpose of developing Korean herbology of the plants to Fumariaceae in Korea, the literatures of the successive generations have been thoroughly investigated to prepare this article.Methods : Books and research papers about Herbology which published at home and abroad were examined.Results : A list was made about Fumariaceae plant which were cultivated or grew naturally in Korea, after investigated the data on domestic and foreign Fumariaceae plants. Out of those lists, serviceable plants were selected and with those available plants, their distributions were analysed and parts which can be used as medicines were divided into 3 categories such as oriental medicine name, scientific name and non-official name. Fumariaceae's properties flavor, channels they use, effects, and toxicity were also noted. Fumariaceae (grew in Korea) were divided into 3 classes with 23 species. Out of those, 2 classes with 9 species were found serviceable which indicates 39% of all. Out of all 23 species of Fumariaceae family, Corydalis genus were found 21 species, which were shown the most. Among Fumariaceae species, Corydalis genus were found 8 medicinal plants, which were shown the most. Out of all serviceable parts in Fumariaceae, Herba and Tuber parts took first place as 5 species. There are 2 toxic species and 3 toxic medicinal parts, the survey said.Conclusion : There were totaled to 3 genera and 23 species in Fumariaceae in Korea and among them medicinal plants are 2 genera, 9 species, some 39 in total.

• 동의생리병리학회지
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• 제17권4호
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• pp.930-938
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• 2003

The purpose of the present study was to investigate the effects of Hyunhosaiksan (HHS) on antithrombotic actions which include blood activation, thrombus removal, warming of circulating blood, and the control of pain on abdomen and lower and upper burning spaces. HHS significantly inhibited platelet aggregation induced by ADP and epinephrine in a HHS dose-dependent manner when analyzed by the Sigmoid Emax model in WinNonlin. EC50 values of HHS were 1.71 ㎍/ml and 0.004 ㎍/ml for ADP and epinephrine respectively. In the vivo study, HHS inhibited pulmonary embolism induced by collagen and epinephrine, which was however statistically insignificant. HHS increased number of platelets, APTT and volume of fibrinogen significantly as compared with the control group in dextran-induced thrombus model. Furthermore, HHS stimulated levels of blood flow in vivo though its effect was not observed in vitro. These results suggest that Hyunhosaiksan (HHS) can be used for treating numerous diseases related with blood aggregation and circulation problems. Further systematic investigations on the synergic effects among drugs used in the oriental medicine as well as in the western medicine in relation to thrombosis therapy would provide an important insight into the potential therapeutic applications.