• Title/Summary/Keyword: 항진균성 활성도

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Detection of Antifungal Endolichenic Fungi and Antifungal Compound (항진균성 지의류 내생 곰팡이 및 항진균성 물질의 탐색)

  • Cheon, Da-Mi;Jang, Da Som;Kim, Hye Young;Choi, Kap Seong;Choi, Sang Ki
    • Korean Journal of Microbiology
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    • v.49 no.2
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    • pp.165-171
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    • 2013
  • To isolate a novel antifungal compound, we obtained 571 kinds of endolichenic fungi from Lichen Bioresources Center and examined their antifungal abilities. Four fungi Stereocaulon sp. (1429), Stereocaulon sp. (1430), Cryptosporiopsis sp. (0156), and Graphis sp. (1245) showed high antifungal activity against Candida albicans when they grew in both liquid and solid media. We extracted the culture supernatants of these fungi with chloroform and then ethyl acetate. The chloroform fraction exhibited the highest anti-fungal activities when those fractions were examined for the growth inhibition of Candida albicans with disc diffusion method. To see information for the inhibitor present in chloroform fraction we employed GC-MS for the fractions of Stereocaulon sp. (1429). We found that hexamethylcyclotrisiloxane, decanoic acid, hexadecanonic acid-methyl ester, 14-octadecenoic acid-methyl ester, and octadecenoic acid-methyl ester were present more in chloroform fraction than in ethylacetate fraction. This indicates that those compounds could be possible antifungal candidates since antifungal activity of chloroform extract was two times higher than that of ethyl acetate extract.

Detection of Endolichenic Fungi Producing Antifungal Compound (항진균성 물질을 생산하는 지의류 내생 곰팡이의 탐색)

  • Kim, Eun-Sung;Choi, Kap-Seong;Choi, Sang-Ki
    • Microbiology and Biotechnology Letters
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    • v.40 no.1
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    • pp.23-29
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    • 2012
  • To isolate a novel antifungal compound, we obtained 100 kinds of endolichenic fungi from Korean Lichen & Allied Bioresources Center and examined their antifungal capability. Three fungi Usnea rigidula (2326), Parmotrema pseudotinctorum (2202) and Myelochroa sp. (2292) showed high antifungal activity against Candida albicans when they grew in both liquid and solid media. We extracted the culture supernatants of these three fungi with chloroform and then with ethyl acetate. Chloroform fraction exhibited the highest antifungal activities when those fractions were examined for the growth inhibition of Candida albicans with disc diffusion method. The chloroform faction was on further analysis with $C_{18}$ column chromatography to see whether the inhibitors are already known or not. Two peak fractions were collected from 4-day culture extract for Usnea rigidula and from 6-day culture extract for Parmotrema pseudotinctorum on the HPLC. A peak fraction from chloroform extracts of 4-day culture filtrate of Parmotrema pseudotinctorum showed higher antifungal activities against C. albicans and C. glabrata than another peak fraction. It appears that the antifungal materials are relatively nonpolar as usnic acid often found in lichenic fungi.

Isolation and Cultural Conditions of an with Antifungal Activity Bacillus sp. (항진균성 활성 Bacillus sp.의 분리 및 생산 조건)

  • Chung, Yong-Joon
    • Korean Journal of Environmental Biology
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    • v.27 no.4
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    • pp.384-390
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    • 2009
  • An antifungal antibiotic-producing bacterium was isolated from soil and identified as Bacillus sp. CJ-1. The culture supernatant was found to have a strong and stable antifungal activity against Colletotrichum gloeosporioides. Culture conditions for the maximum antifungal activity were examined. Glucose and yeast extract were selected as the best carbon and nitrogen sources. The optimum C/N ratio was 3. The optimum temperature and initial pH were determined as $35^{\circ}C$ and 6.0, respectively. Under these conditions, the production for the antibiotic was maximized at 72 hr at $35^{\circ}C$ after cultivation. Microscopic observation showed that the culture supernatant of Bacillus sp. CJ-1 had a strong inhibitory activity on the mycelial growth of the test strain at above $12.5\;{\mu}L\;mL^{-1}$ of concentration.

Production of Antifungal Materials by Bacillus sp. Which Inhibit Growth of Phytophthora infestans and Fusarium oxysporum (Phytophthora infestans와 Fusarium oxysporum의 생장을 저해하는 Bacillus 분리균주들의 항진균성 물질 생성능)

  • Lee, Kang-Hyeong;Song, Hong-Gyu
    • Korean Journal of Microbiology
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    • v.44 no.3
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    • pp.258-263
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    • 2008
  • Late blight, one of the most important disease in many agricultural crops, is caused by Phytophthora infestans. Fusarium wilt is a vascular disease of many plants caused by Fusarium oxysporum. Some bacteria isolated from rhizosphere were screened for their ability to inhibit the growth of F. oxysporum and P. infestans. Productions of siderophore, $\beta-1$,3-glucanase, hydrogen cyanide and chitinase by 4 isolated strains were examined. Among them, Bacillus sp. RFO41 most effectively inhibited the growth of F. oxysporum. The highest productions of siderophore and $\beta-l$,3-glucanase were shown in the culture of Bacillus sp. RFO41. Bacillus strain PS2 was most effective against P. infestans. PS2 showed the highest production of chitinase and hydrogen cyanide. A significant relationship was shown between the antagonistic effects of isolates against F. oxysporum and P. infestans and their production level of siderophore, $\beta-1$,3-glucanase, hydrogen cyanide, and chitinase.

Detection of Antifungal Activities from Pomegranate (석류에서 항진균성 활성의 탐색)

  • Lee, Geum Young;Park, Tae Hee;Lee, Da-In;Park, Jeong-Ro;Choi, Sang Ki
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.44 no.2
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    • pp.287-290
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    • 2015
  • Antifungal activities of pomegranate were investigated. Seventy percent ethanol extracts of seeds, peels, and whole fruits of pomegranate showed similar antifungal activities against Candida albicans in liquid media, whereas extract of whole fruit showed relatively high antifungal activity in solid media. When 70% ethanol extracts were fractionated sequentially with chloroform and ethyl acetate, ethyl acetate fraction exhibited the highest anti-fungal activities against C. albicans. Ethyl acetate fractions of whole fruits and peel portions showed at least 36% and 25% growth against C. albicans, Candida glabrata, Candida tropicalis, and Candida lusitaniae in liquid media, respectively. These results indicate that pomegranate contains antifungal compounds soluble with organic solvents.

An Antifungal Agent Produced by Bacillus thuringiensis BK4, an Antagonistic Bacterium against Fusarium Wilt Disease of Tomato (항진균성 항생물질을 생산하는 Bacillus thuringiensis BK4의 항생물질 정제와 토마토 시들음병의 효과적인 방제)

  • Lim, Jong-Hee;Jung, Hee-Kyoung;Kim, Sang-Dal
    • Applied Biological Chemistry
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    • v.50 no.1
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    • pp.18-22
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    • 2007
  • The optimum production condition for the antibiotic from Bacillus thuringiensis BK4 was determined, and the suppression rate of Fusarium-wilt by the butanol-extracted antibiotic was verified by employing tomatoes in vitro and in vivo pot tests. Cell growth and antifungal activity were the best when 0.5% xylose and 0.2% peptone No.3 were given as carbon and nitrogen sources, respectively, in the presence of 5mM $CaCl_2$. The partially purified antibiotic successfully prevented Fusarium oxysporum pathogen in pot experiments. When the pots were treated with both live cells and the partially purified antibiotic, an additive-effect was seen in the suppression of Fusarium-wilt, but synergistic effect was not detected. The antibiotic, denoted BK4, purified by Sephadex LH-20 column chromatography was eluted with a single peak at a retention time of 38 min. on prep-HPLC; Minimum inhibition concentration of the homogenous antibiotic was determined to be 50${\mu}$g/ml.

Isolation and Structure Identification of Antifungal Substance from Aspergillus terreus (Aspergillus terreus로부터 항진균성 물질의 분리 및 구조분석)

  • Kim, Keun-Ki;Park, Ki-Hun;Moon, Suk-Sik;Kang, Kyu-Young
    • Applied Biological Chemistry
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    • v.40 no.6
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    • pp.593-596
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    • 1997
  • In the course of search antagonistic fungi from soil in green house, four kind of fungi (AF1, AF2, AF3, AF4) were isolated, which have activities against Phytophthora capsici, Botrytis cinera, Rhizoctonia solani, Pythium ultimum and Fusarium oxysporum. The AF2 was identified according to the morphological description of Aspergillus terreus. This antagonistic fungus inhibiting various plant pathogens was effective to reduce disease incidence of cucumber seedlings caused by mixed inoculum of Rhizoctonia solani, Pythium ultimum and Fusarium oxysporum. Antifungal compound I was isolated and purified by fresh chromatography from A. terreus. The $^1H$ and $^{13}C$ assignment of compound I was achieved from two-dimensional $^1H-^1H\;COSY$, HMQC, HMBC with the add of homonuclear and heteronuclear double resonance experiment. The compound I was identified butyrolactone I (${\alpha}$-oxo-${\beta}$-(p-hydroxyphenyl)-${\gamma}$-(p-hydroxy-m-3,3-dimethyl-allylbenzyl)-${\gamma}$-methoxycarbonyl-${\gamma}$-butyrolactone, $C_{24}H_{24}O_7$, M.W.=424).

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Ecofriendly Synthesis of Antifungal Azoles

  • Kidwai, M.;Mohan, R.
    • Journal of the Korean Chemical Society
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    • v.48 no.2
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    • pp.177-181
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    • 2004
  • 1,2,4-Triazoles, pyrazolones and 1,3,4-oxadiazoles have been synthesized from substituted hydrazide using various solid supports under microwave irradiation (MWI). The results obtained highlight the versatility of the solid supports. All synthesized compounds were screened for their antifungal activity against A. niger and A. flavus and were found to possess good activity.

Study on Antifungal Activity of Aloe arborescens M. for a Potential Bio-Pestcide (알로에(Aloe arborescens M.)의 항진균성 검정을 통한 생물농약 가능성 탐색)

  • Ko, Seong-Wook;So, In-Sup;Huh, Moo-Ryong
    • Journal of agriculture & life science
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    • v.43 no.3
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    • pp.35-44
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    • 2009
  • Antifungal activity reaction to Aloe peel and juice extracted by several solvents and concentrations against three plant pathogenic fungi as collectrichum gloeosporioides, Fusarium oxysporum, and F. solani were investigated. The Antifungal activity of Aloe extracts varied with the kinds of solvents. In case of C. gloesporioides and F. solani, it's the highest in water, second in methanol, and third in ethanol. It showed that Antifungal activity of Aloe peel and juice extracts based on concentration was much more excellent in juice extracts than peel extracts against C. gloeosporioides, F. solani. Effect of Antifungal against F. oxysporum by juice extracts did not appear at a concentration of $100{\mu}l/L$ and then shows 9.5% of inhibition rate at $100,000{\mu}l/L$ while against C. gloeosporioides it starts to react at $100{\mu}l/L$ and then reaches at 29.3% of the highest inhibition rate at a concentration of $100,000{\mu}l/L$ out of three plant pathogenic fungi. The effect of control efficacy against Fusarium wilt caused by F. oxysporum by juice extracts of foliar spray tends to increase and the more the concentration of juice extracts increases, the higher the effect of control efficacy against Fusarium wilt disease. Foliar spray at a concentration of 10g/L has a 62% of control efficacy compared with control treatment, which foliar spray of Aloe juice extracts can be the more effective method in order to prevent Fusarium wilt disease caused by F. oxysporum.