Browse > Article
http://dx.doi.org/10.7845/kjm.2013.3023

Detection of Antifungal Endolichenic Fungi and Antifungal Compound  

Cheon, Da-Mi (Department of Biological Sciences, Sunchon National University)
Jang, Da Som (Department of Biological Sciences, Sunchon National University)
Kim, Hye Young (Department of Biological Sciences, Sunchon National University)
Choi, Kap Seong (Department of Food Science and Technology, Sunchon National University)
Choi, Sang Ki (Department of Biological Sciences, Sunchon National University)
Publication Information
Korean Journal of Microbiology / v.49, no.2, 2013 , pp. 165-171 More about this Journal
Abstract
To isolate a novel antifungal compound, we obtained 571 kinds of endolichenic fungi from Lichen Bioresources Center and examined their antifungal abilities. Four fungi Stereocaulon sp. (1429), Stereocaulon sp. (1430), Cryptosporiopsis sp. (0156), and Graphis sp. (1245) showed high antifungal activity against Candida albicans when they grew in both liquid and solid media. We extracted the culture supernatants of these fungi with chloroform and then ethyl acetate. The chloroform fraction exhibited the highest anti-fungal activities when those fractions were examined for the growth inhibition of Candida albicans with disc diffusion method. To see information for the inhibitor present in chloroform fraction we employed GC-MS for the fractions of Stereocaulon sp. (1429). We found that hexamethylcyclotrisiloxane, decanoic acid, hexadecanonic acid-methyl ester, 14-octadecenoic acid-methyl ester, and octadecenoic acid-methyl ester were present more in chloroform fraction than in ethylacetate fraction. This indicates that those compounds could be possible antifungal candidates since antifungal activity of chloroform extract was two times higher than that of ethyl acetate extract.
Keywords
Stereocaulon sp.; antifungal; endolichenic fungi; hexadecanonic acid-methyl ester; 14-octadecenoic acid-methyl ester;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Gehan, M., Abou-Elela, Abd-Elnaby, H., Hassan Ibrahim, A.H., and Okbah, M.A. 2009. Marine natural products and their potential applications as anti-infective agents. World Appl. Sci. J. 7, 872-880.
2 Gonzalez, del Val A., Platas, G., Basilio, A., Cabello, A., Gorrochategui, J., Suay, I., Vicente, F., Portillo, E., Jiménez, del Río M., Reina, G.G., and Pelaez, F. 2001. Screening of antimicrobial activities in red, green and brown macroalgae from Gran Canaria (Canary Islands, Spain). Int. Microbiol. 4, 35-40.
3 Hajji, M., Jarraya, R., Lassoued, I., Masmoudi, O., Damak, M., and Nasri, M. 2010. GC/MS and LC/MS analysis and antioxidant and antimicrobial activities of various solvent extracts from Mirabilis jalaba tubers. Process Biochem. 45, 1486-1493.   DOI   ScienceOn
4 Hashem, M. 2011. Antifungal properties of crude extracts of five Egyptian medicinal plants against dermatophytes and emerging fungi. Mycopathologia 172, 37-46.   DOI
5 John, A.G.R., Steven, M.M., Kim, M., and Jason, Y.J.W. 2006. Determination of usnic acid in lichen toxic to elk by liquid chromatography with ultraviolet and tandem mass spectrometry detection. J. Agric. Food Chemother. 54, 2484-2490.   DOI   ScienceOn
6 Kamer, P., Wincierz, U., Gruber, G., Tschakert, J., Voelter, W., and Mayer, H. 1995. Rational approach to fraction, isolation and characterization of polysaccharides from the lichen Cetraria islandica. Drug Res. 45, 726-731.
7 Koc, A.N., Silici, S., MutluSariguzel, F., and Sagdic, O. 2007. Antifungal activity of propolis in four different fruit juices. Food Technol. Biotechnol. 45, 57-61.
8 Rankoviè, B., Rankovic, D., and Maric, D. 2010. Antioxidant and antimicrobial activity of some lichen species. Microbiology 79, 809-815.   DOI