• Journal of Sericultural and Entomological Science
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• v.49 no.1
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• pp.18-23
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• 2007

The importance of genetic resources conservation and utilization for economy and the health and well-being of societies in general is recognized all over the world. Mulberry genetic resources contain many natural components and are considerable resource for functional sericulture. We carried out antioxidative capacity analysis of mulberry leaves that were collected from Korea and some of foreign countries and preserved in the greenhouse. The mean antioxidant capacity of 34 accessions was 892.30nmol(ascorbic acid equivalent). Also we evaluated fruity characteristics and selected 4 accessions as breeding resources for the production of mulberry fruit. To investigate the effect of tea on antioxidative capacity, five kinds of tea(coffee mix, green tea added brown rice, mulberry leaf tea, Polygonatum odoratum tea and black tea added lemon) were selected and analyzed. Their's antioxidative capacity were 2,531.01nmol, 1,867.42nmol, 1,053.72nmol, 292.71nmol and 188.91nmol, respectively. The antioxidative capacity of drinking water soaked with mulberry leaf showed 891.96nmol.

• Journal of Sericultural and Entomological Science
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• v.49 no.2
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• pp.60-66
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• 2007

This study was carried out to develop the utilization of mulberry resources conserved by Jeollabuk-Do Agricultural Research & Extension Services. Mulberry accessions were tested for agronomic characteristics and antioxidant capacity according to varieties. From that result, three suitable varieties such as 'Sinilppong', 'Suwonsang 2' and 'Ilbongeum' were selected for the production of mulberry leaves. They have strong agronomic characteristics like size, yield and resistance against damages by blight and harmful insects. Whereas, 'Gumunyoung' showed the lowest freezing resistance. In the antioxidant capacity analysis of mulberry leaves, autumn's mulberry leaves showed higher antioxidant capacity (ascorbic acid equivalent) than that of spring's it. According to collection time, antioxidant capacity were 2,109.8 nmol (August), 2,617.8 nmol (September) and 3,311.5 nmol (October), respectively.

• Journal of Sericultural and Entomological Science
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• v.48 no.2
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• pp.41-45
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• 2006

Mulberry leaves contain many natural components and are considerable resource for natural antiokidants. The antioxidant effect of 'Cheongilppong' collected from Chuncheon, Suwon and Wanju regions were investigated with minilum L-100 device and ARAW-KIT (anti-radical ability of water-soluble substance), in comparison to the ascorbic acid. The measurement parts were mulberry leaves collected from different areas and some kinds of mulberry branches. We obtained that even if samples were same variety, antioxidant capacity of those showed different results according to collected regions. Also, antioxidant effect of mulberry leaves were higher than that of branches. The order of antioxidant effect in Spring was Chuncheon > Suwon > Wanju. The antioxidant effect of mulberry leaves collected from Chuncheon, Suwon and Wanju regions of just before the coloration stage with anthocyanin in fruits were 3627.5 nmol, 2620.0 nmol 1360.7 nmol (ascorbic acid equivalents), respectively. In Autumn, the antioxidant effect and hardness quality of mulberry leaves were increased gradually until just before fallen leaves stage. Therefore, we concluded that being the most suitable period for collecting mulberry leaves as a antiokidant resource is from the latter part of May to the first ten days of June.

• Journal of Sericultural and Entomological Science
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• v.47 no.2
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• pp.62-67
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• 2005

Much attention has been focused on the activity of the natural antioxidants present in fruits and vegetables, because potentially these components may reduce the level of oxidative stress. Especially, mulberry leaves containing many natural components are considerable resource far natural antioxidants. The antioxidant capacity of mulberry leaves was investigated with minilum L-100 device and ARAW-KIT (anti-radical ability of water-soluble substance), in comparison to the ascorbic acid. The appropriate conditions for pre-treatments of mulberry leaves were 80% MeOH, 30 sec. vortex mixing. The antioxidant capacity of 16 varieties was 3303.4 nmol (ascorbic acid equivalents) at opening stage of five leaves in spring. The highest stage of antioxidant capacity (3708.0 nmol) and yield rate was just before the coloration stare with anthocyanin in fruits, whereas the lowest stage was middle of June (2231.6 nmol) and about two months growing stage after summer pruning (2064.6 nmol). But after summer pruning, the antioxidant capacity of mulberry.

• Journal of Convergence for Information Technology
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• v.11 no.8
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• pp.232-239
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• 2021

In this study, the antioxidant and anti-inflammatory properties of Silvetia siliquosa extracts were identified. Antioxidant experiments included polyphenol concentration measurements, flavonoid concentration measurements, DPPH experiments, ABTS experiment NO experiments, and FRAP experiments. For polyphenols, 54.85 ± 2.79 mg/g was shown. Flavonoids showed 18.70 ± 5.26 mg/g. The DPPH experiment showed an antioxidant function of 3.950 mg ascorbic acid/g extract, the ABTS experiment showed an antioxidant function of 7.418 mg ascorbic acid/g extract, and the NO experiment showed an antioxidant function of 6.056 mg ascorbic acid/g extract. In FRAP, 1 mg of the moxibustion extract showed a reduction of 3.633 ± 0.280 ㎍ of ascorbic acid. In the meantime, cell experiments showed cytotoxicity and anti-inflammatory functions against inflammation induced by LPS. In cytotoxicity experiments, Silvetia siliquosa extracts showed a cell survival rate of more than 80% at all concentrations, and an inflammatory inhibition of 26.94±0.52% at a concentration of 100 ㎍/mL. These results indicate that Silvetia siliquosa extract is available as an anti-inflammatory cosmetic material.

• Journal of Convergence for Information Technology
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• v.11 no.7
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• pp.264-271
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• 2021

In this study, the antioxidant and anti-inflammatory properties of Sargassum patens extracts were identified. Antioxidant experiments included polyphenol concentration measurements, flavonoid concentration measurements, DPPH experiments, ABTS experiment NO experiments, and FRAP experiments. For polyphenols, 18.99±0.69 mg/g was shown. Flavonoids showed 11.89±1.16 mg/g. The DPPH experiment showed an antioxidant function of 19.78 mg ascorbic acid/g extract, the ABTS experiment showed an antioxidant function of 63.64 mg ascorbic acid/g extract, and the NO experiment showed an antioxidant function of 7.966 mg ascorbic acid/g extract. In FRAP, 1 mg of the moxibustion extract showed a reduction of 2.089 ㎍ of ascorbic acid. In the meantime, cell experiments showed cytotoxicity and anti-inflammatory functions against inflammation induced by LPS. In cytotoxicity experiments, Sargassum patens extracts showed a cell survival rate of more than 80% at all concentrations, and an inflammatory inhibition of 30.64±0.23% at a concentration of 100 ㎍/mL. These results indicate that Sargassum patens extract is available as an anti-inflammatory cosmetic material.

• Journal of Convergence for Information Technology
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• v.11 no.11
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• pp.296-303
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• 2021

In this study, the antioxidant activity and anti-inflammatory activity of Lindera obtusiloba flower fermentation extracts were identified. To evulate antioxidant activity and antioxidant concentration, polyphenol concentration measurements, flavonoid concentrations measurements, DPPH experiments, and ABTS experiments were conducted. In Flavonoids, DPPH and ABTS assay, antioxidant activity were increased after fermentation. At this time, the flavonoid concentration was 5.0%, the DPPH experiment showed 33.27% and the ABTS experiment showed 29.82% antioxidant increase. In the anti-inflammatory experiment, we conducted a cytotoxicity experiment and an anti-inflammatory experiment for LPS-induced inflammation. Cytotoxicity showed low cytotoxicity in both control and fermentation groups, but lower cytotoxicity in fermentation groups. In the case of NO production inhibition, fermented Lindera obtusiloba flowers showed an increase in anti-inflammatory activity by more than 50% compared to the control group, showing that they can be used as a cosmetic ingredient with anti-inflammatory function.

• Journal of the Korean Society of Food Culture
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• v.20 no.1
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• pp.77-85
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• 2005

이 연구에서는 미국산 R. rhabarbarum L.과 중국산 R. palmatum L.의 줄기 추출물과 그리고 한국산 R. undulatum L.의 뿌리 추출물이 천연항산화제로서의 사용 가능성을 평가하였다. 대황의 페놀화합물을 추출하기 위해서 물, 에탄올, 메탄올이 사용되었다. 에탄올이 대황(Rhubarb)에서 페놀화합물을 추출하는데 가장 효과적인 용매였으며 50% 에탄올은 R. rhabarbarum L. 줄기에, 70% 에탄올은 R. palmatum L.의 줄기에, 80% 에탄올은 R. undulatum L.의 뿌리에서 페놀을 추출하는데 각각 가장 효과적인 용매농도였다. 또한 추출물리 항산화능을 알기 위해 ${\beta}-carotene$ bleaching inhibition activity와 DPPH radical scavenging activity를 측정하였으며 합성항산화제인 BHT와 비교하여 측정하였다. ${\beta}-Carotene$ bleaching inhibition activity 실험에서 추출물의 농도가 증가하면 대황추출물의 항산화능이 역시 증가하였다. 추출물의 농도가 5mg/ml와 10mg/ml 일때 R. undulatum L. 뿌리추출물과 BHT의 항산화능은 유의적 차이는 없었다$(p{\leq}0.05)$. 그러나 DPPH radical scavenging activity에서 R undulatum L. 뿌리추출물이 0.4 mg/mL 농도 이상에서는 BHT 보다 높은 항산화능을 나타내었다. 결과적으로 대황(R. undulatum L.)의 80% 에탄올 추출물이 천연항산화제로의 사용이 가능하였다. 그러나 합성항산화제인 BHT의 식품에 사용농도를 비교하여 볼 때 대황 추출물의 항산화능이 BHT와 동일하기 위해서는 보다 높은 농도를 필요로 하였다.

• Microbiology and Biotechnology Letters
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• v.44 no.2
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• pp.117-123
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• 2016

In this study, to evaluate the anti-oxidative and anti-inflammatory effects of Carpinus pubescens Burkill ethanol extract (CPEE), we performed the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging, reactive oxygen species (ROS) inhibition, and nitric oxide (NO) scavenging assays and an analysis of the related protein expressions. CPEE showed high DPPH radical scavenging activity and effectively increased ROS inhibition activity dose-dependently. Furthermore, CPEE induced the expression of the anti-oxidative enzyme heme oxygenase 1 and its upstream transcription factor, nuclear factor-E2-related factor 2, in RAW 264.7 cells. CPEE was associated with a reduction in NO production, which was induced by lipopolysaccharide treatment in a dose-dependent manner. The expression of inducible nitric oxide synthase (iNOS), an upstream regulator of NO production, was also inhibited. Taken together, these results suggest that CPEE has anti-oxidative and anti-inflammatory activities and could be useful as a potential anti-oxidant and antiinflammatory agent.

• Journal of the Korean Society of Food Science and Nutrition
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• v.40 no.7
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• pp.1053-1062
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• 2011

Reactive oxygen species (ROS), including singlet oxygen (${O_2}^1$), superoxide anion radical ($O_2{\cdot}^-$), hydroxyl radical ($HO{\cdot}$), peroxyl radical ($ROO{\cdot}$), hydrogen peroxide ($H_2O_2$), and hypochlorous (HOCl), are generated as byproducts of normal cellular metabolism. ROS induce damage to many biological molecules, such as lipids, proteins, carbohydrates, and DNA. It is widely believed that some degenerative diseases caused by ROS can be prevented by the high intake of fruits and vegetables due to their antioxidant activities. Recently, research on natural antioxidants has become increasingly active in various fields. Several assays have been developed to measure the total antioxidant capacity of antioxidants in fruits and vegetables in vitro. These assays include those for DPPH radical scavenging activity, SOD-like activity, total polyphenol content, oxygen radical absorbance capacity, reducing power, trolox equivalent antioxidant capacity (ABTS assay), single-cell gel electrophoresis (comet assay), and a cellular antioxidant activity assay. Because different antioxidant compounds may act through different mechanisms in vitro, no single assay can fully evaluate the total antioxidant capacity of foods. Due to the complexity of the composition of foods, it is important to be able to measure antioxidant activity using biologically relevant assays. In this review, recently used assays were selected for extended discussion, including a comparison of the advantages and disadvantages of each assay and their application to fruits and vegetables.