• Herbal Formula Science
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• v.31 no.4
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• pp.341-360
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• 2023

Background : To investigate the effect of Hwanggeumjackyak-tang (HJT) on Dextran sulfate sodium (DSS) induced ulcerative colitis. Methods : The experimental animals were divided into three groups; group 1, normal group(Normal); group 2, DSS-induced colitis and untreated group(UT+DSS); group 3, DSS-induced colitis and HJT 200 mg-treated group(HJT200+DSS). We evaluated cytotoxicity after HJT administration and confirmed the anti-inflammatory effect by histological changes in the intestine and genetic analysis of mucosal cells after HJT administration for each group. In addition, microbiological weapons and metabolites in faeces were examined, and the correlation between gut microbiome and metabolites was also investigated. Result : HJT was not observed to be cytotoxic, even at relatively high concentrations, and was effective in protecting the barrier and preventing intestinal inflammation by suppressing the increase in mucus secretion and the expression of inflammatory factors in mucosal cells. HJT treatment affected the increase in the amount and diversity of the gut microbiome in faeces and the increase in metabolites thought to be involved in alleviating inflammation in the gut. Conclusion : This study demonstrates the therapeutic potential of HJT in ulcerative colitis. Further studies should be carried out to confirm our findings.

• Journal of Environmental Science International
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• v.28 no.2
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• pp.183-189
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• 2019

Curcumin ($C_{21}H_{20}O_6$) is a hydrophobic polyphenol found in turmeric. Although curcumin has been used as a natural medicine, its major limitation is related to poor absorption from the gut. Therefore, we developed a method for preparation of Curcumin Nanospheres (CN) to improve the aqueous-phase solubility of curcumin and investigate the functional role of CN in promoting feed efficiency and odor reduction in mice. CN showed inhibitory effects on actate dehydrogenase (LDH) cytotoxicity induced by ecotoxic substance toluene in gut epithelial HCT116 cells. In addition, the weights of internal organs (liver, heart, kidneys, and spleen) and the levels of serum Glutamate Oxaloacetate Transaminase (GOT), Glutamate Pyruvate Transaminase (GPT), and LDH did not show significant differences between mice administered oral CN for two weeks and compared to the control group. Interestingly, CN not only reduced hydrogen sulfide ($H_2S$) and ammonia ($NH_3$) levels and fecal odor, but also improved feed efficiency in mice. These results demonstrate that oral nano-delivery of anti-ecotoxicological CN is a functional system to deliver curcumin to the gut to improve feed efficiency and reduce fecal odor in mice.

• Herbal Formula Science
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• v.32 no.3
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• pp.203-221
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• 2024

Objective: Hyangsanondam-tang (⾹⼭溫膽湯, HSODT) is one of the Korean herbal formula for treatment of insomnia, neurosis and anxiety induced by liver Qi stagnation and unhealthy heart. We investigated the hepatoprotective effect of HSODT against arachidonic acid (AA) + iron-mediated cytotoxicity in HepG2 cells. Methods: AA + iron induced oxidative stress and mitochondrial dysfunction in HepG2 cells. The cytoprotective effects of HSODT were determined by MTT assay, western blot and fluorescence activated cell sorting analysis. Results: In HepG2 cells, pretreatment with HSODT significantly suppressed cytotoxicity and the expression of proteins related to apoptosis induced by AA + iron. In addition, pretreatment with HSODT significantly prevented the increase of H₂O₂ production, and decrease GSH depletion and mitochondrial membrane potential induced by AA + iron. Conclusions : These results indicated that HSODT could protect against oxidative stress-induced liver damage.

• Annual Conference of KIPS
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• 2007.05a
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• pp.369-372
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• 2007

한의학적 진단 이론은 우수성을 보유하고 있음에도 불구하고 계량화, 정량화되어 시각적으로 표현해 주는 결과를 보이지 못해 서양의학에 비해 뒤떨어져 있는 실정이다. 따라서 본 논문에서는 한의학의 망진을 의료 시스템화 하기 위한 한 단계로서 찰색의 기본이 되는 피부색의 분석을 행하였다. 이를 위해 각 개인의 피부색 차이를 수렴하고 이에 대한 분석을 시행함으로 피부색에 대한 공학적 분석을 시도하였다. 본 논문에서는 객관적으로 피부가 검은 사람과 흰 사람을 비교, 분석하였으며 또한 일반인들을 대상으로 피부색 분석을 실행하여 피부색에 대한 개념을 정립하고자 한다. 마지막으로 이에 대한 평균값을 구하며 망진 시스템의 주된 대상이 되는 황인종의 피부색에 대한 연구를 행하고자 한다.

• The Korea Journal of Herbology
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• v.34 no.6
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• pp.117-124
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• 2019

Objective : Broccoli is edible green plant that has a wide variety of health benefits including cancer prevention and cholesterol reduction. However, leaves of broccoli are not eaten and are mostly left as waste. This study was conducted to evaluate the effects of the broccoli leaf extract (BLE) on prostaglandin E₂ (PGE₂) production related to nuclear factor kappa B (NF-κB) signaling in lipopolysaccharide (LPS)-activated macrophages. Methods : BLE was prepared by extracting dried leaf with ethanol. Cell viability was determined by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. PGE₂ and inflammatory cytokines were detected by enzyme-linked immunosorbent assay (ELISA). Expression level of each protein was monitored by Western blot analysis. Results : In LPS-activated Raw264.7 cells, PGE₂ release into culture medium was dramatically enhanced compared to control cells. However, increased PGE₂ was attenuated dose-dependently by treatment with BLE. Inhibition of PGE₂ production by BLE was due to the suppression of cyclooxygenase-2 (COX-2) expression determined by Western blot analysis. BLE also inhibited the production of inflammatory cytokines such as interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α). Inhibition at PGE₂ and cytokine was mediated from inhibition of nuclear translocation of NF-κB due to the repression of inhibitory kappa B alpha (IκBα) phosphorylation and degradation. Conclusion : This study showed that BLE exerted inhibitory activities against PGE₂, which is critical for the initiation and resolution of inflammatory responses, and that inhibition of PGE₂ was mediated by suppression of NF-κB signaling. These results suggest that the waste broccoli leaves could be used for controlling inflammation.

• Herbal Formula Science
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• v.29 no.4
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• pp.181-189
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• 2021

Objectives : The calyx of Diospyros kaki Thunb. has usually been used to treat obstinate hiccup. This study investigated the protective effects of Diospyros kaki using C2C12 myoblasts of H₂O₂-induced oxidative stress. Methods : Cell viability and cytotoxicity were determined by MTT assay. The level of reactive oxygen species (ROS), malondialdehyde (MDA), and reduced glutathione (GSH) were measured by using optical properties. Results : The calyx of Diospyros kaki Thunb. extract showed no toxicity to C2C12 myoblasts until 20 ㎍/mL concentration and increased cell viability compared to the H₂O₂ treated group. The calyx of Diospyros kaki Thunb. extract inhibited the production of ROS and MDA at all concentrations. In addition, the calyx of Diospyros kaki Thunb. extract increased the concentration of GSH. Conclusion : This study provides that the calyx of Diospyros kaki Thunb. can be used as a potential material that exhibit antioxidative and protective effects on H₂O₂-induced oxidative stress in C2C12 Myoblasts.

• Herbal Formula Science
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• v.31 no.3
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• pp.183-191
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• 2023

Objective : UVB damages skin health by causing skin redness and intense inflammation, sunburn, and skin cancer. Paeoniae Radix Alba has been used to relieve gynecological symptoms, muscle spasms, and skin ailments. This study was conducted to confirm whether it has a protective effect against UVB photodamage. Methods : Ethanol extract of Paeoniae Radix Alba (PRA) was prepared by extracting 100 g Paeoniae Radix Alba in 1 L of ethanol for 48 h. Apoptosis was monitored by the terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay and expression levels of apoptosis indicator proteins, and tyrosinase activity was measured with a colorimetric commercial kit. Results : In human keratinocyte HaCaT cells, PRA reduced UVB-induced cell death through apoptosis by inhibiting PARP cleavage and caspase-3 and -9. UVB-induced increase in cellular reactive oxygen species (ROS) was suppressed by PRA pretreatment. PRA also showed dose-dependent ABTS and DPPH radical scavenging activities. Furthermore, the inhibitory effect of tyrosinase activity by PRA was confirmed. Conclusion : These results demonstrated the protective role of PRA in UVB photodamage of human keratinocytes, mainly due to its antioxidant and antiapoptotic properties. We also suggest that PRA can be considered as an effective natural agent to prevent skin photodamage.

• Journal of Environmental Science International
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• v.31 no.1
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• pp.23-31
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• 2022

As a herbal supplement, Dioscorea batatas Decne (DBD) presents potent antioxidant activity and diverse health benefits. In the present study, functions of a 30 kDa glycoprotein isolated from DBD (hereafter, DBD glycoprotein) in the regulation of feed efficiency and fecal malodor in mice were explored. DBD glycoprotein produced protective effect against cytotoxicity induced by the ecotoxicological endocrine-disrupting substance bisphenol A in gastrointestinal epithelial HT-29 cells. To investigate its potential roles in the regulation of feed efficiency and fecal malodor, mice were administered an oral injection of DBD glycoprotein for 2 weeks. Compared with the control values, the weight of internal organs (liver, heart, kidney, and spleen) and levels of glutamate pyruvate transaminase, glutamate oxaloacetate transaminase, and lactic dehydrogenase were not significantly changed during DBD glycoprotein administration for 2 weeks. Interestingly, DBD glycoprotein improved feed efficiency and reduced hydrogen sulfide concentration without altering the ammonia level in mouse feces. Collectively, these results indicate that DBD glycoprotein is a functional agent that exerts gastrointestinal protective effects against ecotoxicological substances, improves feed efficiency, and reduces fecal malodor.

• Annual Conference of KIPS
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• 2008.11a
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• pp.129-132
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• 2008

건강에 대한 관심이 증대되고 있고 이를 만족시키기 위해 한의학과 IT와의 융합을 통한 기술 발전은 필수 불가결한 요소가 되어가고 있다. 최근 한의학의 대중화 및 발전을 위해 여러 방면으로 연구가 진행되고 있다. 본 논문에서는 한의학의 진단법인 4진법 중 가장 뛰어난 망진에 대한 연구의 일환으로 망진의 찰색을 위하여 색 분석을 행하기 위한 영상 획득 환경 중 스트로브 조명을 사용한 색 보정의 효율성을 확인하기 위해 연구를 진행 하였다. 영상 획득 시 다양한 환경에 처할 수 있으므로 스트로브 조명을 통한 일반화 된 실험 환경의 조성이 가능할 수도 있고 혹은 조명이 부족한 상태에서의 보충적인 재료로써의 활용이 가능하기 때문이며 획득된 영상에 색 보정을 실시하여 색 재현성의 효율성을 실제 색 분석을 통해 비교, 분석을 행하였다.

• Journal of the Korean Applied Science and Technology
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• v.40 no.6
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• pp.1567-1579
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• 2023

Sucralose is used as a sucrose alternative in the food sector and is a globally approved pyrogenic, high-intensity artificial sweetener. However, due to the lack of studies on the effects of sweeteners on the brain, this study confirmed whether short-term consumption of sucralose has cognitive and memory protective effects in scopolamine-induced memory-injured animal models. After oral administration of sucralose 2, 5, and 10 mg/kg, scopolamine (1 mg/kg) was administered to the control group and the drug group 30 minutes later, and saline was administered intraperitoneally to the normal group, followed by behavioral experiments As a result of the experiment, Y-Maze, passive avoidance, and Morris WaterMaze recovered more than 10% of cognitive function compared to the control group. In addition, as a result of measuring proinflammatory cytokines, sucralose was found to inhibit IL-6 and TNF-α by more than 30%, and we observed that the expression level of ERK-CREB with intracellular signaling mechanisms increased in a concentration-dependent manner. Therefore, it suggests that sucralose is associated with functional foods for the prevention of functional food patients.