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http://dx.doi.org/10.6116/kjh.2019.34.6.117.

Inhibitory effect of broccoli leaf extract on PGE2 production by NF-κB inhibition  

Park, Sook Jahr (Department of Pharmaceutical Engineering, Daegu Haany University)
An, Iseul (Department of Pharmaceutical Engineering, Daegu Haany University)
Noh, Gyu Pyo (College of Korean Medicine, Daegu Haany University)
Yoo, Byung Hyuk (ARK Inc.)
Lee, Jong Rok (Department of Pharmaceutical Engineering, Daegu Haany University)
Publication Information
The Korea Journal of Herbology / v.34, no.6, 2019 , pp. 117-124 More about this Journal
Abstract
Objective : Broccoli is edible green plant that has a wide variety of health benefits including cancer prevention and cholesterol reduction. However, leaves of broccoli are not eaten and are mostly left as waste. This study was conducted to evaluate the effects of the broccoli leaf extract (BLE) on prostaglandin E2 (PGE2) production related to nuclear factor kappa B (NF-κB) signaling in lipopolysaccharide (LPS)-activated macrophages. Methods : BLE was prepared by extracting dried leaf with ethanol. Cell viability was determined by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. PGE2 and inflammatory cytokines were detected by enzyme-linked immunosorbent assay (ELISA). Expression level of each protein was monitored by Western blot analysis. Results : In LPS-activated Raw264.7 cells, PGE2 release into culture medium was dramatically enhanced compared to control cells. However, increased PGE2 was attenuated dose-dependently by treatment with BLE. Inhibition of PGE2 production by BLE was due to the suppression of cyclooxygenase-2 (COX-2) expression determined by Western blot analysis. BLE also inhibited the production of inflammatory cytokines such as interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α). Inhibition at PGE2 and cytokine was mediated from inhibition of nuclear translocation of NF-κB due to the repression of inhibitory kappa B alpha (IκBα) phosphorylation and degradation. Conclusion : This study showed that BLE exerted inhibitory activities against PGE2, which is critical for the initiation and resolution of inflammatory responses, and that inhibition of PGE2 was mediated by suppression of NF-κB signaling. These results suggest that the waste broccoli leaves could be used for controlling inflammation.
Keywords
Broccoli leaf; $PGE_2$; inflammatory cytokine; NF-${\kappa}B$;
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Times Cited By KSCI : 3  (Citation Analysis)
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