Herbal Formula Science (대한한의학방제학회지)

The Korean Medicine Society for the Herbal Formula Study (대한한의학방제학회)
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Hyangsanondam-tang protects oxidative stress induced-liver damage

산화적스트레스에 대한 향산온담탕(香山溫膽湯)의 간보호(肝保護) 효과

  • Sang Mi Park (College of Korean Medicine, Daegu Haany University) ;
  • Dae Hwa Jung (Department of Pharmaceutical Engineering, Daegu Haany University) ;
  • Sung Hui Byun (College of Korean Medicine, Daegu Haany University) ;
  • Sang Chan Kim (College of Korean Medicine, Daegu Haany University)
  • 박상미 (대구한의대학교 한의과대학) ;
  • 정대화 (대구한의대학교 제약공학과) ;
  • 변성희 (대구한의대학교 한의과대학) ;
  • 김상찬 (대구한의대학교 한의과대학)
  • Received : 2024.07.08
  • Accepted : 2024.08.11
  • Published : 2024.08.31
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Abstract

Objective: Hyangsanondam-tang (⾹⼭溫膽湯, HSODT) is one of the Korean herbal formula for treatment of insomnia, neurosis and anxiety induced by liver Qi stagnation and unhealthy heart. We investigated the hepatoprotective effect of HSODT against arachidonic acid (AA) + iron-mediated cytotoxicity in HepG2 cells. Methods: AA + iron induced oxidative stress and mitochondrial dysfunction in HepG2 cells. The cytoprotective effects of HSODT were determined by MTT assay, western blot and fluorescence activated cell sorting analysis. Results: In HepG2 cells, pretreatment with HSODT significantly suppressed cytotoxicity and the expression of proteins related to apoptosis induced by AA + iron. In addition, pretreatment with HSODT significantly prevented the increase of H2O2 production, and decrease GSH depletion and mitochondrial membrane potential induced by AA + iron. Conclusions : These results indicated that HSODT could protect against oxidative stress-induced liver damage.

Keywords

Acknowledgement

This research was supported by a grant from Daegu Haany University Ky.lin Foundation in 2023.

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