• Journal of Life Science
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• v.27 no.6
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• pp.611-616
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• 2017

The antibacterial activities of nine oriental herbal medicine extracts were measured against seven kinds of bacteria known to cause food poisoning and disease. The extracts were prepared with distilled water using an autoclave ($121^{\circ}C$ for 30 min). The extraction yields of nine oriental herbal medicines were in the rage of 0.5%-33.4%. The antibacterial activities were evaluated using the paper-disc method. Extracts of Hwangryeon, Jiyu, and Ohbaeja showed antibacterial activities while the others did not. Extracts of Coptis japonica, Hwangryeon, and Sanguisorba officinalis, Jiyu showed antibacterial activities only against Clostridium perfringens and Listeria monocytogenes. Extracts of Rhus javanica, Ohbaeja showed the strongest antibacterial activities against all the bacteria tested. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of R. javanica extracts were in the range of 2.4~20 mg/ml for the bacteria tested. In conclusion, the extract of R. javanica showed superior antibacterial activities against the seven bacterial strains tested. The MIC and MBC values for each bacterium were represented as strain names (MIC, MBC), which were Bacillus cereus (11.2, 20), Clostridium perfringens (2.4, 4.7), Escherichia coli (9.1, 9.1), Listeria monocytogenes (11.1, 20), Salmonella typhi (4.7, 9.1), Staphylococcus aureus (2.4, 4.7), and Vibrio parahaemolyticus (2.4, 5.3). Further purification and study of this antibacterial material would be helpful for developing antibiotics or promoting synergistic effects with known antibiotics, producing antibacterial material for lengthening the shelf-life of food, and so on.

• Proceedings of the Korean Journal of Food and Nutrition Conference
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• 2001.12a
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• pp.128-128
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• 2001

전통 한약재의 일환인 황기, 당귀로부터 추출물의 획득은 일반적으로 열수추출법에 의하여 행해져 왔으나 추출물의 획득수율이 낮고 추출, 농축과정에서의 심란 열처리로 인하여 고유의 향이 소실되는 등 많은 문제점을 안고 있다. 이에 본 연구에서는 주정추출법을 시도하여 황기, 당귀 추출물의 획득수율을 열수추출법의 50, 39%(w/w) 대비 각각 15. 36% 증가한 65. 75%로 향상시킬 수 있었으며, 강한 고유의 향을 포집하고 있는 전혀 새로운 타입의 추출물을 얻을 수 있었다. 추출과정on서 열수추출법과의 차이는 추출용매를 물에서 주정으로 변경한 것과 1차 추출 후 얻어진 박을 이용하여 2차 추출을 행하였으며, 농축온도를 열수추출의 104$^{\circ}C$에 비하여 상대적으로 낮은 9$0^{\circ}C$에서 실시하였다. 이와 같이 처리온도와 거의 무관하게 주정은 고유의 향을 포집하는 뛰어난 효과를 나타내었다. 이 추출물을 물엿, 음료 등의 제품에 적용할 경우 별도의 인공향료 처리 없이 천연물 그대로의 가공으로도 충분한 고유의 향미를 부여할 수 있을 것으로 기대된다.

• Microbiology and Biotechnology Letters
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• v.34 no.1
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• pp.70-73
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• 2006

To investigate the potential of treatment, antimicrobial activity of various oriental herb extracts were tested for Gardnerella vaginalis, which is the predominant organism in bacterial vaginosis. Among the tested 14 oriental herbs, water-extracts of Kalkeun, Kosam, Nuro, Pakjakyak, Sukchangpo, Shiyup, Junghyang and Hwangryun represented antibacterial activities against G. vaginalis. The minimal inhibition concentration (MIC) of Shiyup against G. vaginais was 0.63 mg/mL, and those of Pakjakyak and Hwangryun, Kalkeun and Nuro, Kosam, Sukchangpo and Junghyang were 1.25 mg/mL, 2.5 mg/mL, and 5 mg/mL, respectively. There-fore, the water-extracts of Kalkeun, Kosam, Nuro, Pakjakyak, Sukchangpo, Shiyup, Junghyang and Hwangryun were considered to be potential treatment of bacterial vaginosis caused by G. vaginalis.

• Journal of Life Science
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• v.29 no.9
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• pp.1023-1026
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• 2019

The purpose of this study was to investigate the estrogen-like activities of eight oriental medicines, including red ginseng, fenugreek, and dandelion. Hot water and ethanol extracts were prepared from Cervus nippon temminck (Nokgaksang), Cynanchum wilfordii (Baeksuo), Lespedeza cuneata (Yagwanmun), Panax ginseng (red ginseng), Smilax china (Toryeong), Taraxacum platycarpum (Mindeulre, dandelion), Tribulus terresteis (Namgase), and Trigonella foenum-graecum (Horopa, fenugreek). Then, estrogen-like activities were verified by the in vitro transcriptional activity assay. The extracts showing estrogen-like activities were red ginseng, Baeksuo, fenugreek, Yagwanmun, and dandelion in the ethanol extracts, and red ginseng, fenugreek, and Baeksuo in the hydrothermal extracts. Red ginseng extract showed a higher activity than the standard $10^{-8}M$ $17{\beta}$-estradiol in both the hydrothermal and ethanol extract at $500{\mu}g/ml$, while the $50{\mu}g/ml$ of the red ginseng ethanol extract and the $500{\mu}g/ml$ of the Baeksuo hydrothermal extract showed estradiol activities between $10^{-9}$ and $10^{-8}M$ $17{\beta}$-estradiol. This shows that it would be possible to contribute to the development of functional materials using red ginseng, Baeksuo, fenugreek, and dandelion.

• Journal of Food Hygiene and Safety
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• v.16 no.2
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• pp.89-95
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• 2001

This study was conducted to find material having antibacterial activity. The effects of preservatives, antibiotics and oriental medicines on growth of teated microorganisms were investigated. The growth of all tested bacteria was inhibited by water extract of Schizandra chinensis. Antibacterial activity on the concentration of Schizandra chinensis extract was tested. The growth of Escherichia coli W3110, Enterobacter colacae MG82 and Salmonella typhimurium was extraordinarily inhibited by more than 0.2% concentration of Schizandra chinensis extract. The specific growth rate of Escherichia coli W3110, Enterobacter cloacae ME82 and Salmonella typhimurium under control conditin had mean values of 0.514(hr￣), 0.381(hr￣) and 0.489(hr￣), respectively. When 0.2% of Schizandra chinensis extract was added, specific growth rates of Escherichia coli W3110, Enterobacter colacae MG82 and Salmonella typhimurium wre decreased, compared to contorl, in 1.26, 2.23 and 1.50 fold, respectivley. Minimal inhibiotory concentration of Schizandra chinensis extract was 0.25% on the tested microorganisms The growth of Enterobacter cloacae MG82 was more inhibited by Schizandra chinensis extract than other tested microorganisms.

• Journal of Life Science
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• v.20 no.7
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• pp.1143-1150
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• 2010

The antibacterial activity of methanol extracts from 70 kinds of oriental medicinal herbs on four strains of Staphylococcus aureus and S. epidermidis was investigated. The results showed that C. japonica, C. sappan, R. javanica, R. tanguticum, and S. miltiorrhiza had an antibacterial activity on all the strains used. Among these, C. japonica and R. javanica, which showed excellent antibacterial activity, were extracted with water, ethanol, methanol, and ethyl acetate for further study of antibacterial activities. The results showed that the boiled water extract of C. japonica had the best antibacterial activity. Assuming that the antibacterial activity of C. japonica originated from berberine, which has been reported many times, the berberine content of the boiled water extract of C. japonica was analyzed and the result was 13.88%. To compare the antibacterial activity of berberine with that of other antibiotics, berberine chloride and three other kinds of antibiotics were investigated, which showed that berberine chloride had an antibacterial activity on KCCM 35494 S. epidermidis only when the concentration was higher than 600 mg/l, and it did not show antibacterial activity in the other strains. Based on these results, it was concluded that the antibacterial activity of the boiled water extract of C. japonica on the strains used in this study originated from high concentration of berberine or substances other than berberine. Therefore, identification of the substance will be necessary.

• Proceedings of the Korean Fiber Society Conference
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• 2003.04a
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• pp.431-431
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• 2003

건강과 환경에 대한 관심이 고조되면서 이에 따른 생활의 양상도 다양하게 변화되고 있다. 보다 쾌적한 생활 환경을 추구하려는 노력으로 최근 쾌적 섬유의 제조 및 가공에 대한 많은 연구결과가 발표되고 있는 실정이다. 그러나, 합성 항균제를 사용한 항균 가공 직물의 경우 안전성 및 환경친화성에 대한 소비자의 기피현상이 나타나고 있으며, 천연물에 대한 관심이 높아지고 있다. 최근에는 키토산을 비롯하여 양파, 쑥, 계피정유, 및 기타 한약재의 추출물을 사용하여 항균성을 확보하고자하는 연구가 계속되고 있다. (중략)

• The Korean Journal of Pesticide Science
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• v.4 no.3
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• pp.60-67
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• 2000

This study was conducted to elucidate extraction efficiency by microwave in comparison with Soxhlet for extraction of pesticide residues in dried medical herbs; red-ginseng, white-ginseng, Bupleuri Radix, Angelica gigas Nakai, Rehmannia glutinosa. The acetone extraction by microwave of tolclofos-methyl and quintozene in medical herbs was efficient. The extraction efficiency by microwave with power 45 to 150 watts, extraction time 1 to 5 minutes and solvent volume 30 ml was compared with that of Soxhlet with extraction time 7 hours and solvent volume 150 ml. The extraction efficiency by microwave with extraction time 3 to 5 minutes was similar with extraction time of 7 hours by Soxhlet. When medical herbs spiked with tolclofos-methyl and quintozene was analyzed to how the extraction efficiency of microwave by kind of medical herbs, the extraction efficiency by microwave with extraction time of 3 to 5 minutes was the same as Soxhlet extraction. The optimal condition for extraction of tolclofos-methyl and quintozene in medical herbs by microwave was 45 to 90 watts of power supply, 3 to 5 minutes of extraction time and acetone 30 ml of solvent volume.

• Korean journal of food and cookery science
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• v.21 no.6 s.90
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• pp.769-775
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• 2005

In this study we isolate substances to serve as dietary resources in order to replace the female hormone. Silkworm (Bombyx mori) is one of the most attractive hosts for large-scale production of eukaryotic proteins which have been proven safe as a dietary resource. We report on the estrogenicity of a mixture of silkworm pupa and herbs (Ginseng,Ulkeum, and Hasuo) using the immature rat uterotrophic assay in vivo. Silkworm pupa aqueousextract (KW) and silkworm oil extract (KO) induced effects on the immature rat uterotrophic assay. KO showed neither positive uterotrophic response nor inhibition on E2 induced effect, while KW and MK (mixture of KW and herbs) showed both of the effects. It is concluded that ethanol extracts from silkworm might be a good, therapeutic, natural product for hormone-deficient diseases.

• Applied Biological Chemistry
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• v.43 no.2
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• pp.141-147
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• 2000

Interrelation between the antioxidative activity of hot-water extracts of 130 medicinal plants and their cellular antimutagenic activity was investigated. Antioxidative activity was evaluated by assaying electron-donation to DPPH free radical and scavenging of hydroxyl radical $({\cdot}OH)$ generated through Fenton rection, respectively. All medicinal plants examined in this study exhibited markedly electron-donating ability and radical scavenging ability in each assay system. The results demonstrated the fact that Pilbal (Piper longum L.) is the strongest in electron-donating activity, on the other hand, that Seokgok (Dendrobium moniliforme L.) is the strongest in ${\cdot}OH$ scavenging activity. When evaluated their antioxidative activities, 24 medicinal plants including Jimo (Anemarrhena asphodeloides Bunge) were found to be the medicinal plants carrying strong antioxidative activity, which exhibited more than 50% activity compared to the control group in both electron-donating and free radical scavenging. The experiment was also performed to examine whether 11 medicinal plants having significant antimutagenicity damage DNA in the presence of $Cu^{2+}$, showing the fact that all samples tested, except Taeksa (Alisma canaliculatum All. Br.), Paekjain (Nitraria sibirica Pall) and Ohyak (Lindera strychifolia Sieb. et Zucc. Villar) are capable of inducing DNA strand break. We also found that Taeksa and Paekjain strongly block DNA strand break induced by chemical mutagen mitomycin C.