• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.30 no.1
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• pp.106-117
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• 2017

Objectives : 갈근의 피부 보습 효과에 대해 알아보기 위해 본 연구를 진행하였다. Methods : HaCaT 세포주에 갈근 에탄올 추출물을 처리하였고 Retinoic Acid $1{\mu}M$을 양성 대조군으로 사용하였다. MTT assay, RT-PCR, Hyaluronidase enzyme assay, HA-ELISA kit를 통해 Hyaluronic Acid의 합성량, Hyaluronidase 저해율, 농도별 Hyaluronic Acid 생성량을 측정하였다. 더불어 실제로 피부에 도포 시 보습 능력이 있는지 등에 대하여 확인하였다. Results : 갈근 에탄올 추출물은 뚜렷한 세포독성을 나타내지는 않았고, 모든 농도에서 Hyaluronic Acid 합성과 관련된 hyaluronic acid synthase 2 유전자가 발현되었고, 실제로 Hyaluronic Acid가 생성되는 것을 확인하였다. 임상 효능 평가에서는 유의성 있는 결과를 얻을 수는 없었다. Conclusions : 갈근을 한방 외용제로 사용할 때 유의성 있는 효과를 나타낼 수 있을 것으로 기대할 수 있으며, 실험 결과를 토대로 갈근의 최적 사용 농도를 결정하기 위한 추가적 연구와 복합 처방으로 사용했을 경우에 대한 연구가 추가적으로 진행된다면 한방 외용제로서 잠재적인 가치가 있을 것으로 사료된다.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.31 no.2 s.51
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• pp.147-152
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• 2005

As a part of searching tot the natural components which inhibit the skin aging and wrinkle formation, the $80\%$ methanolie extracts of 121 species of traditional herbal medicines used to treat lung and skin disease were investigated for their in vitro anti-oxidative activity against 1,1-diphenyl-2-picrylhydrazyl (DPPH) and superoxide radicals, and inhibitory activity against elastase. We selected 9 kinds of the traditional herbal medicines showing inhibitory activities of winkle formation. The effective concentrations of 9 candidates for anti-wrinke/skin firming activity was less than 0.1 mg/mL, and there is no toxicity to cell viability at these concentrations. Through analysis of human skin primary patch test data, the traditional herbal medicines represented non-irritant materials. We suggest that these 9 candidates with ability to help anti-wrinkle/skin firming may be useful for functional cosmetic materials.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.22 no.2
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• pp.326-335
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• 2021

This study was undertaken to determine the effect of fermented soybean extract and its fractions on skin cell proliferation and growth. The extract was procured by the pepsin and Lactobacillus rhamnosus fermentation of soybean. LC-MS analysis was performed subsequent to soybean fermentation, and cell viability was measured by the WST-1 assay. Cell proliferation was observed to increase after exposing cells to the fermented soybean extract and its fractions at all concentrations tested (0~2,000 ㎍/mL). In particular, compared to the normal control group and 120 % proliferation of the EGF (epidermal growth factor) positive control group, 160~180 % cell proliferation was achieved at 800 ㎍/ml, indicating the excellent potential as an application material for skin aging inhibition and skin cell regeneration. In addition, we also examined the effects of fermented soybean extract and its fractions on wound healing ability, in HaCaT cells and fibroblasts. Our results indicate excellent cell migration abilities after treatment with fermented soybean extract and its fractions, as compared to the control treatment. Similar cell migration abilities were observed in the positive control group (EGF). Taken together, our results indicate that fermented soybean extract and its fractions (F4 and F5) exert amelioratory effects as a natural material for skin.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.49 no.1
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• pp.47-58
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• 2023

Recently, along with the remarkable increase in interest in microbiome cosmetics, the application of microbiome extracts in the complex efficacy as anti-aging, brightening etc. has become very important. In this study, Bifidobacterium bifidum (B. bifidum), which has excellent anti-wrinkle efficacy among the microbiome, was selected through an in vitro test using cells and 3D human skin equivalents. And the anti-wrinkle efficacy of cosmetics containing B. bifidum was evaluated by clinical test. Thereafter, the cytotoxicity, anti-oxidation, anti-inflammatory and anti-wrinkle efficacy of the of the bifida fermented filtrate were tested. An emulsion containing bifida fermented filtrate at a concentration of 5% (v/v) with no cytotoxicity and excellent efficacy was prepared with the placebo emulsion. The clinical test was conducted on a total of 21 women at 2 weeks comparing the bifida emulsion and placebo emulsion. Wrinkles around the eyes were instrumentally evaluated using Antera 3D. The wrinkle reduction rate of the Bifida emulsion group compared with the placebo emulsion group differed by 5.6%. Overall, the selection of microbiome using 3D human skin equivalents and the efficacy study of cosmetics with the microbiome extracts would be actively studied to the development of microbiome cosmetics and skin microbiome mechanisms.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.44 no.3
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• pp.343-347
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• 2018

We synthesized 1, 2-hexanediol galactoside (HD-Gal) from HD using Escherichia coli (E. coli) ${\beta}-galactosidase$ (${\beta}-gal$), in which the reaction is generally called as transgalactosylation (reverse hydrolysis). In this study, we investigated how much HD-Gal and HD had a cytotoxic effect on HaCaT cell, in order to compare HD-Gal with HD in terms of the cytotoxicity of human skin cell. Cell proliferation assay and phase-contrast microscope observation were used for investigating the cytotoxicity. As a result, HD-Gal had not cytotoxic effect on HaCaT cell in the concentration range from 42.2 to 211 mM. In addition, when we observed the cells using microscopy, there was no change in the cell morphology. Meanwhile, when 42.2 mM and 84.4 mM HD were treated on HaCaT cell, we did not observe the cytotoxicity; however, when 168.8 mM and 211 mM HD were on HaCaT cell, HD had a higher cytotoxic effect on HaCaT cell. In addition, when HD was treated on the cells regardless of the concentration of HD, there were obvious changes in cell morphology and cell number. It was expected hopefully that HD-Gal would be applicable as a substitute for HD as a less toxic preservative in views of safety, health, and well-being.

• Korean Journal of Clinical Laboratory Science
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• v.53 no.4
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• pp.363-370
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• 2021

This study was conducted to evaluate the dermal cytotoxicity of lead acetate (LA) and other heavy metal compounds, and the protective effect of Lespedeza bicolor (LB) extract on LA-induced cytotoxicity in cultured B16-/F10 melanoma cells. The study evaluated the antioxidative effects of LB due to its electron-donating ability (EDA), inhibitory effects on melanization and improving cell viability. LA significantly decreased cell viability in a dose-dependent manner, and the XTT₅₀ value was determined at 52.7 µM in the studied cultures. Based on the Borenfreund and Puerner's toxicity criteria, LA was estimated to be highly cytotoxic. LA-induced cytotoxicity and cell damage was reversed by the antioxidant activity of kaempferol (KAE), thereby remarkably improving cell viability. A study of the protective effects of the LB extract on LA-induced cytotoxicity showed that the LB extract remarkably increased cell viability in the LA-treated group, and also inhibited the EDA and the total amount of melanin. The above results suggest oxidative stress-mediated cytotoxicity of LA. In the study, LB extract effectively prevented LA-induced cytotoxicity via its antioxidative activity and inhibition of melanization. In conclusion, natural resources like LB extracts may be useful agents for the prevention of oxidative stress-mediated cytotoxicity and melanization by heavy metallic compounds such as LA.

• Agrochemical news magazine
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• v.24 no.2 s.185
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• pp.9-11
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• 2003

• Proceedings of the Korean Society of Postharvest Science and Technology of Agricultural Products Conference
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• 2003.10a
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• pp.136.2-136
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• 2003

솔잎은 예로부터 민간에서 약용으로 사용되어 간장질환, 비뇨생식기질환, 위장질환, 신경계질환, 순환기질환 및 피부질환 등에 효과가 있다고 알려져 있고 식용으로도 널리 이용하여온 천연식물 자원중의 하나이다. 따라서 본 연구에서는 솔잎 증류액을 가지고 솔잎이 갖는 향기 성분에 대한 분석, 음용수 적합성 시험을 행하였으며, 생리활성 효과를 알아보고자 DPPH 라디칼 소거능 시험, 항돌연변이원성 시험, 세포독성 시험, 유전독성 억제시험을 실시하였다. 실험결과, 향기 성분과 음용수 분석 시험에서는 음용수로 부적합한 성분이 검출되지 않았으며, DPPH 라디칼 소거능 시험에서 RC$_{50}$이 69.9${\mu}\ell$로 높은 항산화 활성을 나타내었다. 또한 항돌연변이원성 시험에서 Salmonella typhimurium TA100 균주에 대하여 시료 농도 200${\mu}\ell$/plate에서 80% 이상의 억제율을 보였다. 세포독성시험 결과, 시료농도 50${\mu}\ell$/well에서 위암세포인 AGS, 간암세포인 HepG2 모두에서 90% 이상의 높은 억제율을 보였다. 그리고 생체내 시험인 유전독성 시험에서는 시료 200${\mu}\ell$/kg에서 72.4%의 비교적 높은 유전독성 억제 효과를 나타내어 기능성 식품의 소재로 사용이 가능 할 것으로 사료된다.

• Environmental Analysis Health and Toxicology
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• v.13 no.3_4
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• pp.95-104
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• 1998

INTRODUCTION : Arsenic is a ubiquitous element present in various compounds throughout the earth's crust. The use of arsenic compounds increased greatly during the 18th and 19th centuries, including its use in pigments and dyes, as a preservative of animal hides, in glass manufacture, agricultural pesticides, and various pharmaceutical substances. The causal association between human arsenic exposure, usually in the form of inorganic compounds containing trivalent arsenite (As$^{III}$) or pentavalent arsenate (As$^V$), and various forms of human cancer has been known for many years.

• Journal of the Korean Applied Science and Technology
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• v.37 no.1
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• pp.56-65
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• 2020

This study attempted to review the possibility of Psidium guajava leaf extract as a cosmetics ingredient by measuring antioxidant activity through ABTS radical scavenging activity, cytotoxicity in RAW 264.7 macrophages, ROS generation-inhibiting effects through DCF-DA assay and antimicrobial activity, and the results found the followings: The Psidium guajava leaf extract revealed excellent ABTS radical scavenging activity. In RAW 264.7 macrophages, no cytotoxicity was found. The ROS generation in the cells was reduced in a dose-dependent manner. The antimicrobial activities were observed in the following strains: S. aureus, E. coli, C. albicans and P. acnes. In terms of minimum inhibitory concentration (MIC) on each strain, which ranged from 0.25 to 1 mg/mL, C. albican was the lowest, followed by E. coli, S. aureus and P. acnes. The above results confirmed the effects of Psidium guajava leaf extract: antioxidant activity, inhibition of ROS generation in the cells, antimicrobial effects on skin flora which causes inflammation. Therefore, it appears that the extract would be available as a cosmetics ingredient which is free of toxins and side effects.