Study on the Moisturizing Effects of Puerariae Radix Ethanol Extract

갈근 에탄올 추출물의 피부 보습 효과에 대한 연구

  • Hong, Chul-Hee (Dept. of Ophthalmology and Otolaryngology and Dermatology, College of Korean Medicine, Sangji University) ;
  • Lee, Sung-Jin (Dept. of Ophthalmology and Otolaryngology and Dermatology, College of Korean Medicine, Sangji University)
  • 홍철희 (상지대학교 한의과대학 한방안이비인후피부과학교실) ;
  • 이성진 (상지대학교 한의과대학 한방안이비인후피부과학교실)
  • Received : 2017.01.11
  • Accepted : 2017.02.18
  • Published : 2017.02.25


Objectives : 갈근의 피부 보습 효과에 대해 알아보기 위해 본 연구를 진행하였다. Methods : HaCaT 세포주에 갈근 에탄올 추출물을 처리하였고 Retinoic Acid $1{\mu}M$을 양성 대조군으로 사용하였다. MTT assay, RT-PCR, Hyaluronidase enzyme assay, HA-ELISA kit를 통해 Hyaluronic Acid의 합성량, Hyaluronidase 저해율, 농도별 Hyaluronic Acid 생성량을 측정하였다. 더불어 실제로 피부에 도포 시 보습 능력이 있는지 등에 대하여 확인하였다. Results : 갈근 에탄올 추출물은 뚜렷한 세포독성을 나타내지는 않았고, 모든 농도에서 Hyaluronic Acid 합성과 관련된 hyaluronic acid synthase 2 유전자가 발현되었고, 실제로 Hyaluronic Acid가 생성되는 것을 확인하였다. 임상 효능 평가에서는 유의성 있는 결과를 얻을 수는 없었다. Conclusions : 갈근을 한방 외용제로 사용할 때 유의성 있는 효과를 나타낼 수 있을 것으로 기대할 수 있으며, 실험 결과를 토대로 갈근의 최적 사용 농도를 결정하기 위한 추가적 연구와 복합 처방으로 사용했을 경우에 대한 연구가 추가적으로 진행된다면 한방 외용제로서 잠재적인 가치가 있을 것으로 사료된다.



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