• Proceedings of the Korean Nutrition Society Conference
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• 2000.06a
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• pp.71-80
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• 2000

• Proceedings of the Korean Society of Crop Science Conference
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• 2003.05a
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• pp.65-65
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• 2003

• Journal of the Korea Academia-Industrial cooperation Society
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• v.15 no.1
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• pp.243-248
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• 2014

This study was carried out to evaluate the economic value of development of separation and purification techniques of flavonoid in citrus using IRR, NPV. The separation and purification techniques of flavonoid of citrus was developed by national institute of horticultural and herbal science in Korea rural development administration. This technology could be used to improve efficiency and practicality of cultivation of citrus. The research results show that the technological value of separation and purification techniques of flavonoid of citrus was evaluated 3,155(scenario 1)~6,518(scenario 2) million won. IRR was 28.3%~61.2%, which was greater than discount rate(11.9%). NPV is 5,541~18,773 million won. Therefore, the economic validity of development of separation and purification techniques of flavonoid of citrus is identified by the results of technology evaluation.

• Journal of Applied Biological Chemistry
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• v.60 no.2
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• pp.185-190
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• 2017

Antioxidative ability of 50% ethanol extract from leaves of Leucaena leucocephala (Lam.) de Wit was investigated. The extraction yield, total polyphenols and total flavonoids of the ethanol extract were $18.3{\pm}0.40%$, $121.3{\pm}11.04{\mu}g$ gallic acid equivalents/mg and $105.2{\pm}2.77{\mu}g$ quercetin equivalents/mg, respectively. The ethanol extract possessed the antioxidative activities such as free radical, cation radical, and nitrite scavenging activities as well as reducing power and lipid peroxidation inhibitory activity. These antioxidative activities of the ethanol extract increased in proportion to the extract concentration. $EC_{50}$ values for free radical scavenging, cation radical scavenging nitrite scavenging, and reducing power were $133.51{\mu}g/mL$, $667.22{\mu}g/mL$, 2.58 mg/mL, and $250.00{\mu}g/mL$, respectively. Polyphenols and flavonoids are suggested to be the key contributors for the antioxidative ability of the ethanol extract from Leucaena leucocephala leaves.

• Journal of the Korean Applied Science and Technology
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• v.34 no.3
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• pp.602-612
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• 2017

This study was performed to verify various effects of 70% ethanol extract and distilled water extract of pine leaf such as antioxidant, anti-inflammatory, skin-lightening effects analysis in cells. The result of antioxidant experiment showed that polyphenol content increased concentration dependently in polyphenol and flavonoid effects. The total content of polyphenols and flavonoids was much higher in ethanol extract than water extract. In all the cells of B16F10, RAW264.7 cytotoxicity at each concentration level didn't appear. The result of measuring NO production inhibition showed that it inhibited NO production led to LPS effectively, so anti-inflammatory activity was confirmed. The result of measuring melanin biosynthesis inhibition showed that there was considerably reduction effect, but it performed western blot, and as a result of MITF, Tyrosinase protein revelation, the inhibition of MITF, Tyrosinase revelation was confirmed concentration dependently, Therefore, in this study pine leaf extract was expected to be used as a cosmetic ingredient.

• Journal of the Korean Society of Food Science and Nutrition
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• v.33 no.1
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• pp.212-217
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• 2004

To make the germinated buckwheat tea, soluble solid contents, total flavonoid contents and organoleptic properties were investigated under various steaming time and drying temperature. The optimum condition of soluble solid contents were 6.93 min of steaming time and 73.59$^{\circ}C$ of drying temperature. Total flavonoid contents were maximum under the condition of 5.22 min of steaming time and 79.05$^{\circ}C$ of drying temperature. The optimum condition of overall palatability was 6.00 min of steaming time and 77.33$^{\circ}C$ of drying temperature. The optimum ranges of soluble solid contents, total flavonoid contents and overall palatability of the tea were 5.4∼7.0 min of steaming time and 75∼8$0^{\circ}C$ of drying temperature. The values expected in the optimum ranges were also similar to the experimental values.

• Journal of Plant Biotechnology
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• v.46 no.4
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• pp.338-345
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• 2019

The selection of a suitable solvent is very important when preparing an extract. However, the effect of ethanol solvent concentration in the extraction of sweet wormwood tea has not been reported. Thus, extracts were prepared from sweet wormwood tea using water and various ethanol concentrations, and the phenolic compounds, antioxidants and anti-enzyme activities of the extracts were analyzed. The phenolic acid and flavonoid components differed according to extraction solvent, which also resulted in different antioxidant and antienzyme activities. In particular, flavonoid rhamnetin was not extracted using 80% and 99.5% ethanol and was highest when 60% ethanol was used for extraction. In the case of chlorogenic acid, the highest extraction efficiency was obtained with 80% ethanol. These results suggest the need for research to increase specific extraction efficiency by targeting major compounds that affect physiological activity.

• Korean Journal of Environmental Agriculture
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• v.36 no.4
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• pp.263-268
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• 2017

BACKGROUND: Lespedeza cuneata G. Don is a well-known medicinal plant. In this study, the biological activities of L. cuneata extracts were investigated. METHODS AND RESULTS: L. cuneata shoot was extracted with 30% ethanol and further fractionated with organic solvents. Total phenolic and flavonoid content, antioxidant activity, and matrix metalloproteinase inhibition effect of the extract and fractions were measured. Among the tested extract and fractions, the highest contents of total phenolic and flavonoids were found in ethyl acetate fraction (117.8 mg GAE/g and 35.9 mg QE/g, respectively). Ethyl acetate fraction showed the highest DPPH and ABTS radical scavenging activity, and the antioxidant activity of the other fractions followed the order n-hexane fraction>ethanol extract>methyl chloroform>n-butanol fraction. Inhibitory effect on the expression of matrix metalloproteinases (MMP1 and MMP3) was highest in the fraction of ethyl acetate, and n-butanol fraction also significantly inhibited the expression of MMP3. Antioxidant activities of L. cuneata extracts were significantly positively related to their phenolic and flavonoid content. CONCLUSION: Ethyl acetate fraction of L. cuneata ethanol extract showed potent antioxidant and matrix metalloproteinases inhibitory activities. Those activities might be related to the high total phenolic and flavonoid content of the extract.

• Journal of Convergence for Information Technology
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• v.11 no.11
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• pp.296-303
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• 2021

In this study, the antioxidant activity and anti-inflammatory activity of Lindera obtusiloba flower fermentation extracts were identified. To evulate antioxidant activity and antioxidant concentration, polyphenol concentration measurements, flavonoid concentrations measurements, DPPH experiments, and ABTS experiments were conducted. In Flavonoids, DPPH and ABTS assay, antioxidant activity were increased after fermentation. At this time, the flavonoid concentration was 5.0%, the DPPH experiment showed 33.27% and the ABTS experiment showed 29.82% antioxidant increase. In the anti-inflammatory experiment, we conducted a cytotoxicity experiment and an anti-inflammatory experiment for LPS-induced inflammation. Cytotoxicity showed low cytotoxicity in both control and fermentation groups, but lower cytotoxicity in fermentation groups. In the case of NO production inhibition, fermented Lindera obtusiloba flowers showed an increase in anti-inflammatory activity by more than 50% compared to the control group, showing that they can be used as a cosmetic ingredient with anti-inflammatory function.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.10a
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• pp.93-93
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• 2019

골관절염은 연골 파괴 및 연골 형성의 손상을 초래하는 산화 스트레스 세포사와 관련된 염증성 질환이다. 최근에, 엉겅퀴(Cirsium japonicum var. maackii)는 다양한 염증성 질환에 대하여 보호하는 역할을 하는 것으로 보고되었습니다. 그러나, 연골 퇴행 및 골관절염 진행에 대한 엉겅퀴의 역할은 아직 알려져 있지 않았다. 따라서, 본 연구는 골관절염 및 연골 분해 생쥐 모델에서 엉겅퀴의 보호 효과를 조사하였다. 먼저, 엉겅퀴의 활성 성분 함량을 측정하기 위해 총 폴리페놀 함량과 총 플라보노이드 함량 분석을 수행하였다. 그 결과, 건조된 엉겅퀴 지상부의 물 추출물에서 총 폴리페놀 함량은 $149.2{\pm}24.1mg\;GAE/g$ 및 총 플라보노이드 함량은 $27.9{\pm}2.0mg\;NE/g$을 함유하는 것으로 밝혀졌다. 또한, HPLC 분석으로부터 엉겅퀴 지상부 물 추출물의 주요 화합물은 플라보노이드 계열인 cirsimarin과 cirsimaritin으로 확인되었다. 또한, 엉겅퀴 추출물은 조직 병리학적 분석에 의해 입증된 내측 반월판의 불안정화에 의해 유도된 골관절염 마우스 모델에서 연골파괴 억제효과를 나타냈다. 결론적으로, 본 연구결과는 엉겅퀴 추출물이 골관절염과 연골 파괴를 개선 또는 예방할 수 있는 새로운 식 의약 소재로의 개발 가능성을 제시한다.