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http://dx.doi.org/10.3839/jabc.2017.030

Antioxidative ability of ethanol extract from the leaves of Leucaena leucocephala (Lam.) de Wit  

Kim, Dong Chung (Department of Integrated Materials Engineering, Chungwoon University)
In, Man-Jin (Department of Human Nutrition and Food Science, Chungwoon University)
Publication Information
Journal of Applied Biological Chemistry / v.60, no.2, 2017 , pp. 185-190 More about this Journal
Abstract
Antioxidative ability of 50% ethanol extract from leaves of Leucaena leucocephala (Lam.) de Wit was investigated. The extraction yield, total polyphenols and total flavonoids of the ethanol extract were $18.3{\pm}0.40%$, $121.3{\pm}11.04{\mu}g$ gallic acid equivalents/mg and $105.2{\pm}2.77{\mu}g$ quercetin equivalents/mg, respectively. The ethanol extract possessed the antioxidative activities such as free radical, cation radical, and nitrite scavenging activities as well as reducing power and lipid peroxidation inhibitory activity. These antioxidative activities of the ethanol extract increased in proportion to the extract concentration. $EC_{50}$ values for free radical scavenging, cation radical scavenging nitrite scavenging, and reducing power were $133.51{\mu}g/mL$, $667.22{\mu}g/mL$, 2.58 mg/mL, and $250.00{\mu}g/mL$, respectively. Polyphenols and flavonoids are suggested to be the key contributors for the antioxidative ability of the ethanol extract from Leucaena leucocephala leaves.
Keywords
Antioxidative ability; Ethanol extract; Flavonoid; Leucaena leucocephala; Polyphenol;
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