• Journal of the Korean Society of Food Science and Nutrition
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• v.33 no.10
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• pp.1653-1660
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• 2004

This study was conducted to investigate seasonal changes of microbiological counts and sanitation state on the surface of food service facilities and utilities of elementary schools in Busan area. Samples swabbed using sponge were collected from the surface of food service facilities and utilities, that is ceiling, wall, floor, trench, grease trap, hood, pest control net and dumb waiter, and analyzed by measuring the total plate, coliform and Salmonella spp. count. The total plate counts at foodservice facilities and utilities except ceiling and wall were ranged from 10$^4$∼10$^{6}$ CFU/100 $\textrm{cm}^2$. The coliform counts were ranged from 10$^2$∼10$^4$ CFU/100 $\textrm{cm}^2$ at foodservice facilities and utilities except ceiling. The grease trap was the severely contaminated facility. It was indicated that the sanitary condition at the foodservice facilities and utilities except ceiling and wall should be improved promptly. As a result of investigating the seasonal changes of microbiological counts, floor, trench, grease trap and dumb waiter should be carried out disinfection before foodserving everyday in summer season, and the disinfection frequencies of wall, hood and pest control net should not be less than three times per week. In winter season floor, trench, and dumb waiter should be carried out disinfection after foodserving, grease trap should be carried out disinfection before foodserving everyday and the disinfection frequencies of wall, hood and pest control net should not be less than twice per week.

• Research in Plant Disease
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• v.14 no.3
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• pp.176-181
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• 2008

This experiment was conducted to find the causes of ineffective seed disinfection methods such as rice seeds soaking in hot water and prochloraz EC solution when the rice seeds were severely infected by Bakanae disease. In case of rice seeds collected from severely diseased field by Bakanae disease, the pathogen as the forms of spores and mycelium were infected in plumule and inner and outer integument of embryo, aleurone layer, and pericarp layer. When the rice seeds were soaked in hot water, the appearances of spores and hypha on the outer pericarp layer were severely disordered, however those of inner region of outer integument and aleurone layer were shown normal. The membrane of hypha on the outer pericarp layer was destroyed within 24 hours, while some spores were healthy and germinated 7 days after soaking, when the rice seeds soaked 24 hours in 125 ppm prochloraz solution at $30^{\circ}C$. These results indicated that the seed disinfection methods were ineffective on the Bakanae disease severely infected rice seed because the hot water did not transmit the pericarp layer of rice seed and also prochloraz solution did not effectively destroy the spore of pathogen.

• Journal of Korean Society of Archives and Records Management
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• v.15 no.2
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• pp.165-179
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• 2015

Archives have historical and academic values. That is why the study of preservation conditions and the environment for the effective management of various records progress. However, materials such as paper and adhesives will inevitably cause biological damages. These damages spread into the inner side of archives, which would make it impossible to recover. For example, archival records from Malaysia and Oman are easily exposed to biological damage because of these countries' hot and humid climate. As such, once records are damaged, disinfection operations are customary in these areas. Methyl bromide (MeBr) and ethylene oxide (EtO) were used in the disinfection process as they showed good insecticidal effect. However their antibacterial effect are negligible. As such, the need for an environmental-friendly fumigator was evident. An environmental-friendly fumigator is designed to improve storage stability. Such equipment is expected to contribute to prevention of damages to cultural heritages through its use of vaporization technology, maintenance of a disinfection space (chamber), and self-generation of nitrogen.

• The Journal of the Korean dental association
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• v.32 no.6 s.301
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• pp.409-416
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• 1994

이 자료는 이전에 공표 되었던 치과계에서의 감염방지 실무에 관한 CDC 권장사항을 개정한 것으로, 새로운 데이터, 재료, 기술, 및 장비 등이 반영되었다. 이러한 권장사항을 이행하면 치과 환경 내에서, 환자에서 치과의료종사자로, 치과의료종사자에서 환자로, 그리고 환자에서 환자로 질병이 전염될 수 있는 위험성을 감소시켜 줄 것이다. 이 문서에는 감염방지의 원칙에 근거하여 다음과 같은 특정 권장사항들이 서술되어 있다. 즉, 치과의료종사자들의 예방접종; 보호용 치장과 방책 기법; 수세와 손의 보호; 날카로운 기구와 바늘의 사용과 관리; 기구들의 멸균이나 소독; 치과진료대 및 주위 표면들의 세척 및 소독; 소독과 치과기공소; 치과진료대의 공기관과 수관에 연결된 핸드피스; 역류방지용 밸브; 기타 구내용 치과 장비 등의 사용과 관리; 한번 쓰고 버리는 기구; 생검 표본의 취급; 치과교육 현장에서 발치 치아를 사용하는 것; 폐기물의 폐기; 권장사항들의 이행.

• Restorative Dentistry and Endodontics
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• v.30 no.2
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• pp.121-127
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• 2005

A variety files made of stainless steel (S-S) or nickel-titanium (Ni-Ti) are used during endodontic treatment. The purpose of tt)is study was to evaluate the corrosion susceptibility of S-S and Ni-Ti endodontic files. Three brands of files were used for this study: $K-flex^{(R)}$ S-S files (Maillefer, USA), $Profile^{(R)}$ Ni-Ti files (Maillefer, USA), $K-3^{(R)}$ Ni-Ti files (SybronEndo. USA). 120 files of each brands (21mm, ISO size $\#20$) were divided into 12 groups according to 1) sterilization methods using Autoclave or Ethylene Oxide (E-O) gas, 2) Irrigation solutions using $5.25\%$ NaOCl or Saline, 3) the number of sterilization (1, 5, 10 times), After above procedures, each of the files was inspected by three examiners with a light microscope and camera at X25. Each file was judged and ranked according to the following criteria: 0;, no corrosion, 1; mild corrosion, 2; moderate corrosion, and 3; severe corrosion. The files of high score were examined under the Scanning Electron Microscope. Data were statistically analyzed with the Kruskal-Wallis test (p < 0.05). Most of the ten time-autoclaved files had showed mild to moderate corrosion. But, one or five time-autoclaved files did not show corrosive surface. NaOCl treatment and E-O gas sterilization did not influence on corrosion. There was a significant difference in corrosion susceptibility between sterilization methods and the number of autoclaving. However, there was no significant difference between brands and file materials.

• Journal of Food Hygiene and Safety
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• v.25 no.3
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• pp.238-244
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• 2010

Currently, in vitro suspension tests using tubes are used as a authorized test method for sanitizers and disinfectants. However, the methods could not accurately assess the efficacy of sanitizers and disinfectant on the food-contacted surfaces in the field. This study evaluated the effectiveness of 5 kinds of representative sanitizers and disinfectants against E. coli and S. aureus to compare three quantitative surface testing methods that have been internationally standardized. As a result, the ASTM E2111-05 (ASTM(1)) test method obtained 5.18 $\pm$ 0.03 and 5.27 $\pm$ 0.04 log cfu/carrier reduction in dealing with E. coli and S. aureus, respectively, the ASTM E2197-02 (ASTM(2)) test method obtained 4.63 $\pm$ 0.04 and 3.97 $\pm$ 0.03 log cfu/carrier reduction and the CEN EN 13697 test method should 6.14 $\pm$ 0.05 and 5.31 $\pm$ 0.10 log cfu/carrier reduction in clean condition (CEN(1)) but 4.37 $\pm$ 0.02 and 4.06 $\pm$ 0.01 log cfu/carrier reduction in dirty condition (CEN(2)). Among them, CEN(1) showed the highest bactericidal effects, whereas ASTM(2) and CEN(2) revealed low performance (p < 0.05). In conclusion, the bactericidal effects of the ASTM(2) method and the CEN EN 13697 method adopting stainless steel were lower than the ASTM(1) method, which uses glass. The effectiveness assessment results among nationally accredited test methods were different each other. This implies that they could not fit for in the accurate evaluation of sanitization and disinfection on food-contact surfaces in practical food-processing fields. These results could be used as a basic data for establishment of an official surface test methods applicable in the field.

• Research in Plant Disease
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• v.30 no.2
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• pp.194-198
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• 2024

Pathogens of fire blight can survive for a long time on pruning tools, etc., and fire blight can be spread through agricultural implements. In particular, in Korea, pruning is frequently performed to remove flowers, immature fruits, and succulent shoots, and this farm work is known to be a major factor of the spread of fire blight. Therefore, in this study, in order to completely sterilize pathogens on pruning shears, we verified the disinfection effect of disinfectants distributed domestically and used them to identify an effective disinfection method. When disinfecting by immersion after inoculating Erwinia amylovora TS3128 on the scissor blade, 70% ethanol and 1% and 0.2% sodium hypochlorite sterilized immediately 10 sec after immersion, while 30% chlorine dioxide showed little disinfection effect. When disinfecting by spraying, 70% ethanol sterilized bacteria 1 sec after spraying, but 1% and 0.2% sodium hypochlorite disinfected bacteria after 10 and 60 sec, respectively. After cutting the naturally disease occurring branches five and 100 times, the bacteria were not hardly sterilized in immersion treatment in 70% ethanol for 30 sec but perfectly disinfected over 60 sec. Considering these results, pruning shears should be disinfected by frequently immersing them in 70% ethanol for at least 60 sec during pruning work in the field.

• Journal of the korean veterinary medical association
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• v.50 no.2
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• pp.104-111
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• 2014

안구표면은 자연스럽게 형성되는 세균집단의 거처로 항시 소독이 되어 있지 않은 상태로 존재한다고 봐야 한다. 예방 또는 치료를 목적으로 사용되는 적절한 항생제를 선택하려면 안구표면에 항시 산재하는 세균들에 관한 것은 물론 병원균에 효력이 있는 약품 그리고 그 약품의 부작용에 관한 사항들을 숙지해야 한다는 것은 수의사로서 자명한 사실이다. 이 글은 문헌들을 토대로 하여 개와 고양이 안구부(眼口部)에 서식하는 세균 그리고 병원균들과 그에 응용해야 할 항생물질들을 사용하는 데 조금이라도 도움이 될 수 있도록 간단히 분류 적절하게 이용할 수 있기를 바라는 마음에서 정리한 것이다.

• Journal of Food Hygiene and Safety
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• v.26 no.3
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• pp.203-208
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• 2011

This study was to evaluate the efficacy of sanitizer concentrations and treatment time against two major toad-borne pathogenic microorganisms such as Escherichia coli and Staphylococcus aureus on a stainless steel surface. As a result, stainless steel, treated with 100 ppm of chlorine showed reduction of E. coli(1.56, 1.49, 1.95 log cfu/25 $cm^2$) and S. aureus(0.49, 0.88, 1.27 log cfu/25 $cm^2$) after 0, 5 and 10 min, but none was not detected in treatment with 200 ppm. The population of E. coli(0.73, 0.90, 1.55 log cfu/25 $cm^2$) and S. aureus(0.37, 1.00, 1.45 log cfu/25 $cm^2$) reduced in 35.5% ethanol treated group, but none was not detected in treatment with 70%. The population was reduced E coli(0.28, 0.64, 1.07 cfu/25 $cm^2$) and S. aureus(0.53, 0.87, 0.99 log cfu/25 $cm^2$) by treatment with 45.5 ppm of hydrogen peroxide, but none was not detected in treatment with 91 ppm. Quarternary ammonium compound with 100 ppm was reduced E. coli(0.82, 1.62, 1.71 log cfu/25 $cm^2$) and S. aureus(0.46, 0.93, 1.38 log cfu/25 $cm^2$), but none was not detected in treatment with 200 ppm. Predictive models of sterilization for all 4 disinfectants were suitable to use with $r^2$ value of higher than 0.94. These models may be of use to food services and manufacture of safe products by controlling E. coli and S. aureus without the need for further detection of the organisms.

• Korean Journal of Food Science and Technology
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• v.44 no.2
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• pp.240-246
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• 2012

This study evaluated the efficacy of strong acidic electrolyzed water (SAcEW), low alkaline electrolyzed water (LAlEW) and aqueous chlorine dioxide (ACD) for reducing pathogenic bacteria($Escherichia$ $coli$, $Bacillus$ $cereus$, $Salmonella$ Typhimurium, $Stapylococcus$ $aureus$) on Chinese cabbage. Artificially inoculated Chinese cabbage was immersed for 1, 5 and 10 min with TW, NaClO, EW and ACD. Generally, leaves showed more effective reduction than stems. Regarding the inhibitory effect, ACD treatment showed the highest effects rather than other treatments. When Chinese cabbage was immersed for 3 min in sterilized water, it was reduced to a minimum of 1.33 log CFU/g at LAlEW and a maximum of 4.70 log CFU/g at ACD. Compared to NaClO, ACD and LAlEW which showed a reduction of 3.2 log CFU/g ($Sal.$ Typhimurium) and 2.7 log CFU/g($B.$ $cereus$), respectively. Furthermore, the others had similar inhibitory effects compared to NaClO.