• Transactions of the Korean Society of Mechanical Engineers B
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• v.37 no.3
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• pp.249-257
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• 2013

Rapid prototyping was used to design and develop a three-dimensional (3D) scaffold for tissue engineering application. In this study, the nozzle guide (TB-CP-HN, MUSASHI ENGINEERING, INC., JAPAN) used with the syringe of the polymer deposition system (PDS) was evaluated by measuring the scaffold line width and height. 3D scaffolds were fabricated using a biodegradable polymer called poly-caprolactone (PCL). The PCL polymer can be deposited from the needle of a syringe using a 200-${\mu}m$ precision nozzle, at a pressure of 600 kPa and temperature of $125^{\circ}C$. The advantages and improvements in this nozzle guide were addressed through washer scaffold fabrication. Overall, this research indicated that the fabrication of a complex-shaped scaffold using an enhanced polymer deposition system may have potential for tissue engineering.

• Journal of the Korean Society for Precision Engineering
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• v.29 no.6
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• pp.686-692
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• 2012

Solid free-form fabrication (SFF) technology was developed to fabricate three-dimensional (3D) scaffolds for tissue engineering (TE) applications. In this study, we developed a polymer deposition system (PDS) and created 3D microstructures using a bioresorbable polycaprolactone (PCL) polymer. Fabrication of 3D scaffolds by PDS requires a combination of several devices, including a heating system, dispenser, and motion controller. The system can process a polymer with extremely high precision by using a 200 ${\mu}m$ nozzle. Based on scanning electron microscope (SEM) images, both the line width and the piled line height were fine and uniform. Several 3D micro-structures, including the ANU pattern (a pattern named after Andong National University), $45^{\circ}$ pattern square, frame, cylindrical, triangular, cross-shaped, and hexagon, have been fabricated using the polymer deposition system.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.39 no.12
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• pp.1229-1235
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• 2015

Scaffold fabrication technology using a 3D printer was developed for damaged bone tissue regeneration. A scaffold for bone tissue regeneration application should be biocompatible, biodegradable, and have an adequate mechanical strength. Moreover, the scaffold should have pores of satisfactory quantity and interconnection. In this study, we used the polymer deposition system (PDS) based on fused deposition modeling (FDM) to fabricate a 3D scaffold. The materials used were polycaprolactone (PCL) and alginic acid sodium salt (sodium alginate, SA). The salt-leaching method was used to fabricate dual pores on the 3D scaffold. The 3D scaffold with dual pores was observed using SEM-EDS (scanning electron microscope-energy dispersive spectroscopy) and evaluated through in-vitro tests using MG63 cells.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2011.06a
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• pp.1459-1460
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• 2011

• Journal of the Korean Society for Precision Engineering
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• v.31 no.9
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• pp.791-797
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• 2014

Abstract Scaffold used as a carrier of the cell has been actively conducted using plenty of technology in tissue engineering. ${\beta}$-tricalcium phosphate (${\beta}$-TCP) material has shown good biocompatibility and osteoconductive ability when it was implanted as a bone graft substitute in osseous defect in human and animal studies for bone regeneration. In this study, we fabricated the blended polycaprolactone (PCL) and ${\beta}$-TCP scaffold by the polymer deposition system (PDS). The PCL/${\beta}$-TCP scaffold was fabricated at a temperature of $110^{\circ}C$, pressure of 650 kPa, and scan velocity of 100 mm/sec. The Overall geometry and size of the scaffold were fixed circle type with a diameter of 10 mm and a height of 4 mm. PCL/${\beta}$-TCP scaffold was observed by scanning electron microscopy. Cell attachment and proliferation of the scaffold containing 30 wt% ${\beta}$-TCP was superior to those containing 10 wt% and 20 wt% ${\beta}$-TCP.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.39 no.11
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• pp.1153-1159
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• 2015

Recently, synthetic biopolymers and bioceramics such as poly (${\varepsilon}$-caprolactone)(PCL), hydroxyapatite, tricalcium phosphate, biphasic calcium phosphate(BCP), and zirconia have been used as substrates to generate various tissues or organs in tissue engineering. Thus, the purpose of this study was the characterization of $ZrO_2$/BCP/PCL(ZBP) scaffold for bone tissue regeneration. Based on the result of single-line test, blended 3D ZBP scaffolds with fully interconnected pores and new complex pore pattern of $45^{\circ}+135^{\circ}$-type and staggered-type were successfully fabricated using a polymer deposition system. Furthermore, the effect of ZBP scaffold on mechanical property was analyzed. In addition, in vitro cell interaction of ZBP scaffold on MG63 cells was evaluated using a cell counting kit-8(CCK-8) assay.

• Journal of the Korean Society for Precision Engineering
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• v.26 no.12
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• pp.123-130
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• 2009

This study presents the application of a polymer behavior model that considers fluid mechanics and heat transfer effects in a deposition system. The analysis of the polymer fluid properties is very important in the fabrication of precise microstructures. This fluid behavior model involves the calculation of velocity distribution and mass flow rates that include the effect of heat loss in the needle. The effectiveness of the proposed method was demonstrated by comparing estimated mass fluid rates with experimental values. The mass fluid rates under various process conditions, such as pressure, temperature, and needle size, reflected the actual deposition state relatively well, and the assumption that molten polycaprolactone(PCL) is a non-Newtonian fluid was reasonable. The successful fabrication of three-dimensional microstructures demonstrated that the model is valid for predicting the polymer behavior characteristics in the microstructure fabrication process. The results of this study can be used to investigate the effect of various parameters on fabricated structures before turning to experimental approaches.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.28 no.6A
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• pp.861-872
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• 2008

Concrete-filled glass fiber reinforced polymer tubes are often used for marine structures with the benefit of good durability and high resistance against corrosion under severe chemical environment. Current research presents results of a comprehensive experimental investigation on the behavior of axially loaded circular concrete-filled glass fiber reinforced polymer tubes. This paper is intended to examine several aspects related to the usage of glass fiber fabrics and filament wound layers used for outer shell of piles subjected to axial compression. The objectives of the study are as follows: (1) to evaluate the effectiveness of filament winding angle of glass fiber layers (2) to evaluate the effect of number of GFRP layers on the ultimate load and ductility of confined concrete (3) to evaluate the effect of loading condition of specimens on the effectiveness of confinement and failure characteristics as well, and (4) to propose a analytical model which describes the stress-strain behavior of the confined concrete. Three different types of glass fiber layers were chosen; fabric layer, ${\pm}45^{\circ}$ filament winding layer, and ${\pm}85^{\circ}$ filament winding layer. They were put together or used independently in the fabrication of tubes. Specimens that have various L:D ratios and different diameters have also been tested. Totally 27 GFRP tube specimens to investigate the tension capacity, and 66 concrete-filled GFRP tube specimens for compression test were prepared and tested. The behavior of the specimens in the axial and transverse directions, failure types were investigated. Analytical model and parameters were suggested to describe the stress-strain behavior of concrete under confinement.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.41 no.7
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• pp.645-653
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• 2017

In bone tissue engineering, polycaprolactone (PCL) is one of the most widely used biomaterials in the manufacturing of scaffolds as a synthetic polymer having biodegradability and biocompatibility. The strut width in the fabrication of scaffolds is an important part of tissue regeneration in in-vitro and in-vivo experiments, because it affects not only the pore size but also the porosity. In this study, we used polymer deposition system (PDS) and design of experiments (DOE) to explore the optimal process conditions to achieve a systematic and efficient scaffold manufacturing process, using temperature, pressure, scan velocity, and nozzle tip height as the parameters for the experiments. The aim of this research was to fabricate a 3D PCL scaffold having a uniform strut width of $150{\mu}m$ using DOE; it was proved that the strut width was constant in all the experimental groups by fabricating the PCL scaffolds according to various pore patterns as well as one pore pattern.

• Journal of the Korean Society of Manufacturing Process Engineers
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• v.15 no.5
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• pp.86-92
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• 2016

In this study, we used a polymer deposition system, based on fused deposition modeling, to fabricate the 3D scaffold and then fabricated micro-pores on a 3D scaffold using a salt leaching method. Materials included polycaprolactone (PCL) and sodium chloride (NaCl). The 3D porous scaffolds were fabricated according to blending ratio such as PCL (70 wt%)/NaCl (30 wt%) and PCL (50 wt%)/NaCl (50 wt%). The 3D porous scaffolds were observed by scanning electron microscopy. The results showed that 3D porous scaffolds had a deposition width of $500{\mu}m$, contained a pore size of $500{\mu}m$ and below $100{\mu}m$. To evaluate the 3D porous scaffolds for bone tissue engineering, we carried out the cell proliferation experiment using a CCK-8 and a mechanical strength test using a universal testing machine. In summary, the 3D porous scaffold was found to be suitable for cancellous bone of human in accordance with the result of in-vitro cell proliferation and mechanical strength. Thus, a 3D porous scaffold could be a promising approach for effective bone regeneration.