• Journal of the Society of Cosmetic Scientists of Korea
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• v.48 no.4
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• pp.365-372
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• 2022

Cellobiose is a dissacharide constituted by two glucose units joined by a β-('1,4') glycosidic bond that is produced by the decomposition of cellulose. This product exists naturally in plants and has been utilized in different industries as a food sweetener, and as a cosmetic and pharmaceutical material. In this study, the potential of cellobiose as a whitening cosmetic product was evaluated by analyzing autophagy induction and the inhibition of melanin production. A cytotoxicity test conducted in the human melanin-producing cell line MNT-1 with increasing concentrations of cellobiose revealed that this compound did not cause cytotoxicity at 20 mg/mL or less. Based on this, autophagy was firstly evaluated by immunostaining with the autophagy marker microtubule-associated protein 1 light chain 3 (LC3) after treatment with 20 mg/mL of cellobiose. The subsequent confocal microscopy analysis revealed an increase in LC3 puncta, indicating induction of autophagy. In addition, autophagy was further confirmed by western blot analysis, which demonstrated that cellobiose converted LC3-I to LC3- ∏ in a concentration- and time-dependent manners. An analysis of melanin contents after cellobiose treatment at a concentration of 20 mg/mL during 7 days revealed that melanin production was reduced by more than 50%. Additionally, the expression levels of melanogenesis-related proteins TYR and TYRP1 were markedly decreased after cellobiose treatment. Based on these studies, a cosmetic cream formulation containing cellobiose was prepared and the change in formulation was tested for 4 weeks, and it was confirmed that the appearance changed to liquid form at high temperature, but the pH did not change. In conclusion, the present research demonstrated that cellobiose activates autophagy and inhibits melanin production, and showed the potential of this product as a material for whitening cosmetics.

• Journal of Life Science
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• v.33 no.3
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• pp.242-251
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• 2023

The purpose of this study was to investigate the effects of 8-week β-hydroxybutyrate (β-HB) administration with and without endurance exercise training on endurance exercise performance and skeletal muscle protein synthesis and degradation using a mouse model. Forty-eight male wild-type ICR mice (8 weeks old) were randomly divided into four groups: sedentary control (Sed+Con), (Sed+Con), sedentary β-HB (Sed+β-HB), exercise control (Exe+Con), and exercise β-HB (Exe+β-HB). β-HB was dissolved in PBS (150 mg/ml) and injected subcutaneously daily (250 mg/kg) for 8 weeks. Mice performed 5 days/week of a 20 min treadmill running exercise for 8 weeks. The running exercise was carried out at a speed of 10 m/min at a 10° incline for 5 min, and then the speed was increased by 1 m/min for every 1 min of the remaining 15 min. Following 8 weeks of treatments, visceral fat mass and skeletal muscle mass, blood parameters, and the markers for autophagy and protein synthesis were analyzed. The data were analyzed with one-way ANOVA (p<0.05) using the SPSS 21 program. Eight weeks of Exe+β-HB treatment significantly lowered blood lactate levels compared with the other three groups (Sed+Con, Sed+β-HB, and Exe+β-HB) Exe+β-HB) (p<0.05). Eight weeks of Exe+β-HB significantly increased maximal running time (time to exhaustion) compared with the Sed+Con and Exe+Con groups (p<0.05). Eight weeks of β-HB administration significantly decreased autophagy flux and autophagy-related proteins in the skeletal muscle of mice (p<0.05). Conversely, the combined treatment of β-HB and endurance exercise training increased protein synthesis (mTOR signaling and translation) (p<0.05). The 8-week β-HB treatment and endurance exercise training had synergistic effects on the increase in endurance performance, increase in protein synthesis, and decrease in protein degradation in the skeletal muscle of mice.

• Landscaping Tree
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• s.102
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• pp.26-28
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• 2008

최근 환경변화와 국제교류의 확대로 인하여 예전에는 문제가 되지 않았거나, 보이지 않았던 해충들이 문제해충화 되어 우리 주변에서 정원수나 조경수에 피해를 주는 해충들이 있다. 이들은 크게 3가지로 분류되는데 (1)환경의 변화에 의한 잠재해충과 문제해충화 (2) 인간의 욕심에 의해 대면적 식재로 인한 해충의 기회 확대 (3) 국제교류 확대로 인한 외래 해충의 침입 으로 나눌 수 있다. 이번 호에서는 환경변화에 의한 해충의 발생으로 환경스트레스에 의해 식물 자체 내의 방어체계가 무너지면 어떤 특정종이나 일반 해충이 대발생하는데 식물자체 환경 스트레스에 관계하는 jasmonate의 작용 저해, 식물이 자기방어를 위해 분비하는 타감물질(allelopathy)의 분비 억제, 초식성 곤충으로부터 산란억제를 위한 연모의 부족 등 해충의 대발생을 야기 시키는 경우도 있다. 또한 지구온난화로 인한 각종 해충의 대발생은 현재 전 세계적으로 문제가 되고 있다. 기온의 상승으로 곤충의 발육이 빨라져 화기 수가 증가한다거나, 포식자와 피식자의 상호작용의 연결고리가 깨어지는 등 각종 원인으로 인한 최근 발생한 해충을 기술하였다.

• Journal of environmental and Sanitary engineering
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• v.15 no.1
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• pp.1-6
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• 2000

A milky gray scum was observed at some water area of the lake Daecheong in the summer of 1997 and 1998. To identify a causing organism of scum and affecting factors, we observed the scum material by a phase contrast microscope and surveyed the phsico-chemical water quality during the outbreak of scum. The scum was found out to be clogging cysts of amoeboid protozoan, Asterocaelum sp.(Protozoea Sarcodina Aconchulinida), grazer of filamentous cyanobacteria, Anabaena genus. The protozoan scum appeared during Anabaena bloom period, which continued for a while. This protozoan was presumed acting as a regulator of Anabaena bloom in the lake Daecheong during the summer season. Moreover this is the first report on Asterocaelum sp. a grazing filamentous cyanobacteria occurred in Korean freshwater.

• Korean Journal of Microbiology
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• v.20 no.1
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• pp.21-26
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• 1982

The disinfection effects of sunlight and predacious microorganisms on enteria of Escherichia coli and Proteus vulgaris were examined in sewage water at 22 to $26^{\circ}C$. The test prganisms were disinfected by 4 to 5 logs during 6-hour a day exposure to sunlight for seven days. The effect was proportional to the light intensity and was proved to be dur to the action of the visible light of short wavelength which can transmit the soft glass of a flask glass. The predacious microorganisms decreased trhe test organisms by about 3 to 4 logs during 7 days. The complex actions of sunlight and predacious microorganisms were variable depending upon the test organisms, but the total effects of those enviromental factors were yet completely understood.

• Proceedings of the Korean Society of Computer Information Conference
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• 2013.07a
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• pp.307-310
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• 2013

아무르불가사리에 기인한 환경적, 경제적 피해는 해마다 증가되고 있습니다. 우리나라 남해와 동해 바다에 널려 있는 불가사리들이 최근 서해안에서도 급속히 늘어나고 있습니다. 특히 러시아 해역에 서식하는 아무르 불가사리의 개체수가 급증해 어장을 황폐화 시키고 있습니다. 이로 인하여 엄청난 번식력과 포식력을 지닌 아무르불가사리가 살아있는 조개류를 먹어치우고 있어서 어민들의 피해가 막심합니다. 이 논문은 변형되어진 아무르불가사리의 구조적 특징과 방사대칭 특성을 이용하여 불가사리 영상을 복원하는 복원기법으로 불가사리의 인식률을 높게 하여 불가사리 퇴치에 많은 도움이 될 것으로 기대합니다.

• Journal of Environmental Science International
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• v.15 no.4
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• pp.385-386
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• 2006

In 2005, harmful dinoflagellate Cochlodinium polykrikoides was first occurred on July 18 and disappeared on September 12 in the Yeosu waters of the South Sea of Korea. During C. polykrikoides blooms, the species of Protoperidinium was isolated from Jabong Island in Yeosu in the middle of August, 2005. The surface water temperature at the sampling site was $25^{\circ}C$, and salinity was 33.1 psu. The specimen was somewhat large in size and was longer than its width. The shape was close to being ovoid, with an apical horn. This study discovered cells with colorless and transparent ingested chain-forming C. polykrikoides. However, this species was not abundant, implying low grazing impact on C. polykkoides.

• Journal of the Korean Institute of Intelligent Systems
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• v.14 no.2
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• pp.195-200
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• 2004

Computer games use an intelligent method called flocking for boids' group behavioral modeling. Flocking can naturally model group behavioral patterns of unpredictable forms such as birds and fishes using some computer resource. In this paper, we implemented an ecosystem which is composed of predator and prey for group behavioral modeling of real underwater ecosystem. Also fuzzy logic is applied to implement instinct desire of ecosystem elements. As the result, we confirmed that the model can overcome breakdown of ecosystem and model naturally ecosystem behavior.

• Journal of environmental and Sanitary engineering
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• v.19 no.2
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• pp.51-59
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• 2004

강우가 집중하고 남조류가 대발생하던 시기의 중영양호소인 주암호에서 박테리아, 식물플랑크톤, ANF, HNF, 섬모충플랑크톤, 동물플랑크톤과 환경요인들의 변화에 대해 조사하였다. 조사기간동안 우점종은 Cyclotella meneghinana, Aulacoseira granulata, Cryptomonas tetrapyrenoidsa로서 총현존량의 67% 이상을 차지하였다. 조사기간동안 남조류 대발생의 구성원은 현존량 및 종조성에서 크게 변화 하였다. 여름철 강우에 의한 엽록소 a, 수질 (COD, TP)의 변동이 심하였으며, 특히 C. tetrapyrenoidsa는 HNF, nauplii, rotifer, 섬모충의 변동에 매우 밀접한 관계를 보였다. 포식자인 rotifer는 박테리아, 피코플랑크톤이나 ANF같은 소형플랑크톤의 변동과 높은 관계를 보였다.

• Proceedings of the Korean Society of Fisheries Technology Conference
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• 2000.05a
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• pp.361-362
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• 2000

해양의 대형무척추동물은 어류의 먹이가 되고, 서식처나 먹이를 놓고 어류와 경쟁을 하며, 심지어 어류를 포식하는 등 어류 군집 구조에 많은 영향을 주는 생물적 요인이 된다. 새우류는 절지동물문 갑각목 십각아목에 속하는 무척추동물이며, 국내에서 이루어진 새우류 군집에 관한 연구는 광양만 잘피밭에 서식하는 새우류 군집의 종조성과 계절 변동 (Huh and An, 1997), 고리 주변 해역 새우류의 종조성과 계절 변동(Huh and An, 1999)이 있을 뿐 아직까지 우리나라 연안에서 출현하고 있는 새우류의 군집 연구가 부족한 상태이다. 본 연구에서는 가덕도 주변 해역에서 출현하는 새우류 군집의 종조성과 계절변동을 파악함으로써 상위 영양단계를 점유하고 있는 어류 군집을 연구하는데 기초 자료를 제공하고자 하였다. (중략)