• Research in Plant Disease
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• v.14 no.3
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• pp.219-222
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• 2008

Real time PCR was used to discriminate Colletotrichum acutatum and C. gloeosporioides for analysis of population density. Two primers, caInt2 and cgint, used for conventional PCR to discriminate two species were modified with fluorescent dye to make probe for real time PCR. Fluorescence signals were successfully detected by fCaInt2 and vCgint probe coupled with primer pair Unicon and Unicor1 resulting in discrimination of C. acutatum and C. gloeosporioides by comparison of delta Rn value.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.10a
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• pp.53-53
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• 2018

본 연구는 대승 경전에 나타난 식물의 수록 양상과 그 의미를 밝히는 연구의 세 번째 과정이다. 법화경은 천태종(天台宗)의 소의 경전(所依慶典)으로 우리나라에서 유통된 경전 가운데 최다 간행된 경전이며 화엄경과 함께 한국 교학의 중심이 된 경전이다. 연구 대상은 현존하는 법화경의 판본 가운데 구마라집(鳩摩羅什) 역 묘법연화경(妙法蓮華經, 이하 법화경, 7권으로 구성)에 수록된 식물이다. 본 연구에서는 식물 분류학적 실체는 물론 이 경의 중심 논지와 연관성이 큰 천화(天華, 하늘의 꽃)의 실체를 별도로 구명하여 식물수록의 특성과 차별성을 동시에 구명하고자 하였다. 1. 법화경에 나타난 식물의 수록 횟수와 식물의 종 수는 다음과 같았다. 법화경에는 총 27종의 식물이 수록되어 있었으며 이 가운데 목본은 16종(59.3%)이고 초본은 11종(40.7%)이었다. 식물수록 양상에 있어 특이점은 목본이 초본에 비해 상대적으로 높은 비중을 차지하고 있었다는 점이었다. 이러한 결과는 중심사상의 전달방식에 있어 비교 대상이 되는 다른 대승 경전과 차이가 있다는 점과도 무관하지 않았다. 실제로 비교 대상인 화엄경의 목본과 초본 비율은 각각 17종(48.6%)과 18종(51.4%)이었고 열반경은 각각 36종(45.6%)과 43종(54.4%)으로 목본과 초본의 수록 비율에 차이가 크지 않았다. 2. 법화경에 나타난 식물 27종은 총 20개 과에 분포되어 있었다. 수련과 3종, 콩과 3종, 물푸레나무과 2종, 뽕나무과 2종, 벼과 2종의 순이었다. 다수 과에 분포된 식물은 대부분 중심사상 전달의 매개체인 천화(하늘의 꽃)에 속하는 식물이었다. 3. '하늘의 꽃' 이라고 신성시되면서 이 경의 내용 전개에 있어 중요한 위치를 점하고 있는'천화(天華)'는 연꽃과 수련 종류를 지칭하는 식물이 중심이 되었으며 유용한 관상 및 조경, 향료 자원 및 목재 자원 식물이었다.

• Journal of Life Science
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• v.9 no.6
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• pp.671-676
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• 1999

RAPD analysis using random primers were tried to evaluate the genetic variation and diversity of the nine garlic cultivars including two foreign varieties. Thirty-two primers out of 70 primers screened were used to amplify genomic DNA of garlic cultivars using polymerase chain reaction(PCR). Among a total of 151 bands amplified by 32 primers, 125 polymorphic bands were subjected to analysis for genetic relationship of garlic cultivars. The estimated size of amplified PCR products were in the range of 932 to 4,060 base pairs. Nine garlic cultivars were classified into two groups, such as group I corresponded to Changnyung and Hungary cultivars, and group II, Namdo, Sandong from China, Yecheon, Euiseong, Youngweol, Danyang, Jeongsun cultivars, with the genetic distance value of 0.271. The major ecological types of garlics, so called southern and northern types, was grouped in the genetic distance value of 0.200. The results presented in this study suggest that RAPD analysis are likely to be useful for identification of cultivars and evaluation of genetic origin in garlics.

• Proceedings of the Turfgrass Society of Korea Conference
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• 2005.04a
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• pp.31-38
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• 2005

본 실험은 한국잔디 신품종 '세녹'(특허출원:10-2003-0072018) 개발에 관한 것이다. 한국에서 수집한 유전자원 중 갯잔디와 금잔디간에 인공교배를 수행해서 F1계통을 얻었다. 작성한 F1계통을 포장상태에서 방임수분을 통해 변이확대를 하였으며, 이들 방임수분 후대에서 선발한 것이 '세녹'이다. '세녹'은 유전적으로 잔디의 녹색도가 진하며, 낮게 자라는 특성이 있고 품질이 우수하며 잎의 강직성이 높다. 엽폭이 3.1mm로 중엽형이며, 초장이 14cm로 낮고, 잎각도가 67.3도로 넓다. 지면으로부터 최하위 엽의 잎 몸 기부까지의 길이가 2cm로 낮은 형태적 특성을 갖고 있어 낮은 잔디깍기에 적응할 수 있는 영양번식형 잔디이다. 또한 RAPD 밴드 특성에서 한국잔디 신품종 세녹(Senock)이 특이 밴드를 갖고있어 식별성이 높다.

• Journal of Yeungnam Medical Science
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• v.7 no.1
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• pp.145-149
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• 1990

Sclerosing hemangioma of the lung is uncommon benign neoplasm of uncertain histogenesis. Their radiologic appearance is relatively distinct and well defined. Recently we experienced 3 cases of sclerosing hemangioma of the lung in 54, 52, 51 years old women. The light microscopic findings are similar to the features reported by Liebow and Hubbell(1956).

• The Korean Journal of Mycology
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• v.31 no.3
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• pp.121-128
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• 2003

This study was carried out to identify the phylogenetic relationship of Phellinus species and to know its distribution by comparing the DNA sequences of internal transcribed spacer regions(ITS1 and IST2) and 5.8S ribosomal DNA (rDNA) repeat unit. The Phellinus species had their specific sequences in IST1 and 2 regions depending on suedes. The comparison of the ITS sequences of standard strains indicated that the sequences of ITS1 were more variable than those of ITS2. Nine strains of the commercial products of Phellinus species used in this study were identified as P. lintues, P. baumii, P. igniarius, and P. pini. Most of commercial species were P. pini and P. baumii, and P. gilvus was not found. Also, P. linteus was only found in form of mycelial culture rather than fruiting body. Moreover, the species-specific primers were designed based on ITS sequence data. Each species-specific primers were bound in P. lintues(ITSF-PL2R), P. baumii(PB1F-ITS4R), P. igniarius(IF1-IR3), P. pini(PF1-PR3), and P. gilvus(GF2-GR4), respectively. These primer sets would be useful fer the detection of specific-species among unidentified Phellinus species rapidly.

• Journal of the Korean Society of Marine Environment & Safety
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• v.21 no.1
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• pp.1-8
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• 2015

We investigated the effect of monochromatic light emitting diode (LED) on the growth of diatoms Nitzschia sp., Phaeodactylum tricornutum, Skeletonema sp. and green algae Chlorella vulgaris. The four microalgae species were cultured under blue LED (450 nm), yellow LED (590 nm), red LED (650 nm) and fluorescent lamp (mixed wavelengths). The maximum growth rates and cell densities of Nitzschia sp., P. tricornutum and Skeletonema sp. were highest under blue LED, followed by fluorescent lamp, red LED and then yellow LED, however those of C. vulgaris were highest under red LED. This result indicates that blue LED is favorable for the growth of diatoms. Thus, the growth of microalgae under monochromatic light might be species-specific or taxon-specific. Also, these results could be used as an important information in future for remediation of heavy metal contamination in the sediments using LED and microalgae.

• Korean Journal of Poultry Science
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• v.36 no.4
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• pp.317-322
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• 2009

The chicken major histocompatibility complex (MHC) is known to be associated with disease resistance and susceptibility to several pathogens. The microsatellite marker LEI0258 is physically located between the BG and BF of MHC region and variations near this marker have been well documented. In this report, the LEI0258 marker was used to find specific alleles for the Korean native chicken. The MHC haplotype was analyzed by PCR screening and sequencing of LEI0258 region in four different breeds including black Korean native chicken, brown Korean native chicken, Cornish and Rhode island red. The serologically same MHC haplotypes showed the differences in repeat numbers, a few indels or single nucleotide polymorphisms by sequencing analysis. Even though we could not identify specific alleles for Korean native chickens, the genotypes analyzed in these breeds can give valuable information for the relationships with disease resistance and establishment of breeding strategies for the Korean native chicken.

• Journal of Plant Biotechnology
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• v.45 no.2
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• pp.102-109
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• 2018

Thistle is a perennial plant that is widely used for medicinal purposes. Information on the genetic diversity of thistle populations are great important for their conservation and germ plasmic utilization. Although thistle is an important medicinal plant species registered in South Korea, no molecular markers are currently available to distinguish them from other similar species from different countries. In this study, we developed single nucleotide polymorphism (SNP) markers derived from the nuclear ribosomal DNA internal transcribed spacer (ITS) regions of genomic sequences to identify distinct Korean-specific thistle species via an amplification refractory mutation system (ARMS)-PCR and high resolution melting (HRM) curve analyses. We performed molecular authentication of four different kinds of thistle species from different regions using DNA sequences in the ITS intergenic region. We also developed a quantitative PCR assay using species-specific ITS primers, which allowed us to estimate the ratio of Korean-specific thistle species using varying ratios of mixed genomic DNA templates from the two species. The SNP markers developed in this study are useful for rapidly identifying specific thistle species from different countries.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.50 no.spc1
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• pp.191-195
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• 2005

Experiment was conducted to establish the standard of quality evaluation in Korean cultivated peony roots. free sugars, free amino acids, organic acids and aromatic components, which were generally considered to be information components of five sensory test in peony roots, were examined. As free sugars, sucrose, glucose and fructose were identified in peony roots. $\gamma-aminoisobuturic$ acid, arginine and other 16 kinds of free amino acids were found in peony roots. The major organic acids of peony roots were oxalic acid, citric acid and malic acid. Eugenol and other 10 aromatic components were identified in peony roots by GC/MSD.