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http://dx.doi.org/10.4489/KJM.2003.31.3.121

Taxonomical Classification and Species-specific Detection of Genus Some Phellinus using Phylotype  

Kim, Cheng-Yun (Department of microbiology, College of Natural Sciences, Pusan National University)
Lee, Jae-Yun (Department of microbiology, College of Natural Sciences, Pusan National University)
Kim, Gi-Young (Department of microbiology, College of Natural Sciences, Pusan National University)
Lee, Ki-Won (Department of microbiology, College of Natural Sciences, Pusan National University)
Park, Jae-Min (Department of microbiology, College of Natural Sciences, Pusan National University)
Kim, Mun-Ok (Department of microbiology, College of Natural Sciences, Pusan National University)
Lee, Tae-Ho (Department of microbiology, College of Natural Sciences, Pusan National University)
Lee, Jae-Dong (Department of microbiology, College of Natural Sciences, Pusan National University)
Publication Information
The Korean Journal of Mycology / v.31, no.3, 2003 , pp. 121-128 More about this Journal
Abstract
This study was carried out to identify the phylogenetic relationship of Phellinus species and to know its distribution by comparing the DNA sequences of internal transcribed spacer regions(ITS1 and IST2) and 5.8S ribosomal DNA (rDNA) repeat unit. The Phellinus species had their specific sequences in IST1 and 2 regions depending on suedes. The comparison of the ITS sequences of standard strains indicated that the sequences of ITS1 were more variable than those of ITS2. Nine strains of the commercial products of Phellinus species used in this study were identified as P. lintues, P. baumii, P. igniarius, and P. pini. Most of commercial species were P. pini and P. baumii, and P. gilvus was not found. Also, P. linteus was only found in form of mycelial culture rather than fruiting body. Moreover, the species-specific primers were designed based on ITS sequence data. Each species-specific primers were bound in P. lintues(ITSF-PL2R), P. baumii(PB1F-ITS4R), P. igniarius(IF1-IR3), P. pini(PF1-PR3), and P. gilvus(GF2-GR4), respectively. These primer sets would be useful fer the detection of specific-species among unidentified Phellinus species rapidly.
Keywords
Gene probe; Phellinus; Phylogentic analysis;
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