• 제목/요약/키워드: 탐색적 연구

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Modulation of Cellulalr Quinone Reductase Inducibility by Roasting Treatment and Acid Hydrolysis of Perilla (들깨의 볶음처리와 산가수분해에 의한 세포모델계 Quinone Reductase 활성유도능의 변화)

  • Hong, Eun-Young;Kang, Hee-Jung;Kwon, Chong-Suk;Nam, Young-Jung;Suh, Myung-Ja;Kim, Jong-Sang
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.26 no.2
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    • pp.186-192
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    • 1997
  • Increased activities of phase 2 enzymes including quinone reductase(QR) have been reported to be associated with protection of animals from neoplastic, mutagenic, and other toxic effects of many carcinogens. In previous study, we found that methanol extract of roasted and defatted perilla meal induced the activity of quinone reductase, an anticarcinogenic marker enzyme, in murine hepalc1c7 cells. Current study showed that unroasted perilla had a limited QR-inducing activity, suggesting that roasting cause the generation of active component(s). Thus we hypothesized that QR inducer in perilla might be covalently linked to sugar moiety and released during roasting process. Methanol extract of defatted raw perilla was subject to acid treatment in order to hydrolyze the potential sugar moiety. Prolonged hydrolysis of methanol extract of defatted raw perilla at $98{\sim}100^{\circ}C$ increased the ability to induce cytosolic QR activity of hepalclc7 cells. Furthermore roasting at 180 and $200^{\circ}C$ resulted in significant induction of QR activity. The result strongly support the idea that QR inducer(s) is present in bound form in raw perilla and released during roasting. Cellular QR activity was induced proportionately with the increase of concentration of methanol extract of roasted perilla. The induction of QR by defatted perilla was also examined in the cytosols of liver, small intestine, stomach, lung and kidney of male ICR mice. Induction patterns showed specificity with respect to target tissue and roasting of perilla. Unroasted perilla meal (defatted) significantly induced QR in liver and lung, while roasted perilla meal induced QR in liver and stomach. The observation that raw perilla showed similar QR induction patterns to roasted perilla is consistent with our proposal that QR inducer(s) is present in bound form and released by physical and chemical treatments as digestive or microbial enzymes could release the inducers from inactive glycoside forms in gastrointestinal tract of mice. In conclusion, perilla could exert protective effect against chemically induced carcinogenesis by inducing phase 2 enzymes in biological systems regardless of chemical and physical process such as roasting.

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Genetic Variations of Chicken MC1R Gene and Associations with Feather Color of Korean Native Chicken (KNC) 'Woorimatdag' (토종 '우리맛닭' 부계 및 실용계에서 MC1R 유전자 변이 및 모색과의 연관성 분석)

  • Park, Mi Na;Kim, Tae-Hun;Lee, Hyun-Jeong;Choi, Jin Ae;Heo, Kang-Nyeong;Kim, Chong-Dae;Choo, Hyo-Jun;Han, Jae-Yong;Lee, Taeheon;Lee, Jun-Heon;Lee, Kyung-Tai
    • Korean Journal of Poultry Science
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    • v.40 no.2
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    • pp.139-145
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    • 2013
  • There are several loci controlling the feather color of birds, of which one of the most studied is Extended black (E) encoding the melanocortin 1-receptor (MC1R). Mutations in this gene affect the relative distribution of eumelanin, phaeomelanin. The association of feather color and sequence polymorphism in the melanocortin 1-receptor (MC1R) gene was investigated using Korean native chicken H breed (H_PL) and 'Woorimatdag' commercial chickens (Woorimatdag_CC). In order to correlate gene mutation to Korean native chicken feather color, single nucleotide polymorphism (SNP) from MC1R gene sequence were investigated. A total of 307 birds from H_PL and Woorimatdag_CC were used. H_PL have black, black-brown feather color and Woorimatdag_CC have black with brown spots or brown with black spots. There are 6 SNPs in MC1R gene, locus T69C, C212T, A274G, G376A, G636A, T637C. 3 SNPs are nonsynonymous that change amino acid. But it is difficult to find correlation of feather color and polymorphisms. It will be needed to increase the population of Korean native chicken H breed and correlation analysis of genetic variation with feather colors.

Effects of SSI Argumentation Program based on SEL for Preservice Biology Teachers (예비 생물교사를 위한 사회정서학습에 기반한 SSI 논증 프로그램 적용 효과 탐색)

  • Kim, Sun Young;Kim, Su Hyeon
    • Journal of The Korean Association For Science Education
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    • v.38 no.2
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    • pp.259-271
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    • 2018
  • This study examined the effect of the SSI argumentation program based on social and emotional learning(SEL). The program consisted of 3 stages: (1) express their own feelings about SSI, identify the issues of SSI, and define a goal; (2) think of many possible solutions and envision results through argumentation; (3) select the best solution and make a decision based on warrants, data, and rebuttals. In each stage, the social-emotional strategies of self-awareness, self-management, social-awareness, relationship-management, and responsible decision making were used. Seventeen preservice biology teachers participated in this study during one semester dealing with four socioscientific issues. The results indicated that the preservice teachers, as time went on, became accustomed to expressing identifiable rebuttals, dispute talk, and asking questions. At the first SSI argumentation, argumentation mainly consisted of cumulative talk with no rebuttals, representing level 2 argumentation. Level 3 argumentation represented rebuttals that were implicit and weak, with cumulative talk. In level 2 and 3 argumentation, the preservice teachers represented understanding of others and compassion for self and others. Level 4 argumentation had rebuttals that were explicit, asking critical questions of the opposite sides. In addition, level 5 argumentation represented more than two controversial points with several instances of dispute talk. In levels 4 and 5, the preservice teachers became actively engaged in communication, inquiry self with others, managing vulnerability and negotiation.

Development of the evaluation tool for the food safety and nutrition management education projects targeting the middle class elderly: Application of the balanced score card and the structure-process-outcome concept (중산층 노인대상 식품안전·영양관리 교육 사업 평가를 위한 도구 개발: 균형성과표와 구조·과정·성과 개념 적용)

  • Chang, Hyeja;Yoo, Hyoi;Chung, Harim;Lee, Hyesang;Lee, Minjune;Lee, Kyungeun;Yoo, Changhee;Choi, Junghwa;Lee, Nayoung;Kwak, Tongkyung
    • Journal of Nutrition and Health
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    • v.48 no.6
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    • pp.542-557
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    • 2015
  • Purpose: The aim of this study is to develop an evaluation tool for operation of food safety and nutrition education projects for middle class elderly using the concept of the balanced score card. Methods: After the draft of the evaluation tool for the elderly training projects was completed, it was revised into the questionnaire and the validity of the indicators was tested by the Delphi group. The validity of the indicators was rated using a 5-point scale. The Delphi group consisted of 26 experts in the education sector, 16 government officials, and 24 professionals of the related area in communities. The first round test was conducted from July 9 to July 17, 2012, and 45 persons responded. The second round test was conducted from July 18 to July 25 and 32 persons responded. Results: The indicators, which were answered by more than 75 percent of the experts as 'agree' (4 points), 'strongly agree' (5 point) were included as the final indicators for the evaluation tool: 28 items out of 36 in outcome perspectives, 9 items out of 12 in process perspectives, and 17 out of 20 items in structure perspectives. The score was allocated as 50 points for outcome indicators, 20 points for process indicators, and 30 points for structure indicators. Conclusion: Completion of the evaluation tool is a prerequisite to determine whether the program is effectively implemented. The monitoring tool developed in the study could be applied for identification of the most optimal delivery path for the food safety and nutrition education program, for the spread of the food safety and nutrition education program for middle class elderly.

Fertilizing Effects of Swine Compost Fermented with Sawdust on Mixed Pastures (혼파초지에 대한 톱밥발효돈분의 시용효과)

  • Shin, J. Soon;Cho, Young-Mu;Lee, Hyo-Ho;Yoon, Sea-Hung;Park, Geun-Je;Choi, Ki-Chun
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.24 no.3
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    • pp.245-252
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    • 2004
  • Experiment was carried out to find the fertilizing effects of 8 different application rates of swine compost fermented with sawdust(SCS) including Chemical fertilizer(CF) on forage yield and soil chemical characteristics of mixed pastures sown in Sep. 1993 at National Livestock Research Institute, RDA., in Suwon during low years. It was arranged in a randomized complete block design with three replicates. Dry matter yield were shown at similar among treatments except Control and $50\%$ SCS of standard amount plot. In botanical composition, the legume and weeds percentages of each treatment were increased as advancing year. The final year's legume percentage were high in line with SCS fertilizing plots($39\%{\sim}43\%$), SCS + CF plots($30\%{\sim}41\%$) and CF plot($32\%$). In productions of TDN, NE and crude protein yield, SCS or SCS($75\%$) + CF($25\%$) were nearly same comparing those of CF, respectively. Phosphate, potassium, magnesium contents and K/(Ca + Mg) except calcium contents of those SCS fertilizing plots in plant were generally high with comparing CF. Those contents were proportional according to the fertilizing amount These result indicate the possibility to substitute chemical fertilizer for SCS($75\%$, 25ton/ha) + CF, $25\%$) as manure-N 210 kg/ha, but might be considered accumulation phosphate in the soil.

A study on the improving and constructing the content for the Sijo database in the Period of Modern Enlightenment (계몽기·근대시조 DB의 개선 및 콘텐츠화 방안 연구)

  • Chang, Chung-Soo
    • Sijohaknonchong
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    • v.44
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    • pp.105-138
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    • 2016
  • Recently with the research function, "XML Digital collection of Sijo Texts in the Period of Modern Enlightenment" DB data is being provided through the Korean Research Memory (http://www.krm.or.kr) and the foundation for the constructing the contents of Sijo Texts in the Period of Modern Enlightenment has been laid. In this paper, by reviewing the characteristics and problems of Digital collection of Sijo Texts in the Period of Modern Enlightenment and searching for the improvement, I tried to find a way to make it into the content. This database has the primary meaning in the integrating and glancing at the vast amounts of Sijo in the Period of Modern Enlightenment to reaching 12,500 pieces. In addition, it is the first Sijo data base which is provide the variety of search features according to literature, name of poet, title of work, original text, per period, and etc. However, this database has the limits to verifying the overall aspects of the Sijo in the Period of Modern Enlightenment. The title and original text, which is written in the archaic word or Chinese character, could not be searched, because the standard type text of modern language is not formatted. And also the works and the individual Sijo works released after 1945 were missing in the database. It is inconvenient to extract the datum according to the poet, because poets are marked in the various ways such as one's real name, nom de plume and etc. To solve this kind of problems and improve the utilization of the database, I proposed the providing the standard type text of modern language, giving the index terms about content, providing the information on the work format and etc. Furthermore, if the Sijo database in the Period of Modern Enlightenment which is prepared the character of the Sijo Culture Information System could be built, it could be connected with the academic, educational contents. For the specific plan, I suggested as follow, - learning support materials for the Modern history and the national territory recognition on the Modern Age - source materials for studying indigenous animals and plants characters creating the commercial characters - applicability as the Sijo learning tool such as Sijo Game.

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A Study on the Change of Production Performance of 5 Strains of Korean Native Chicken after Establishment of Varieties (한국재래닭 5계통의 종 조성 후 생산능력 변화 추이에 관한 연구)

  • Kim, Ki Gon;Kang, Bo Seok;Park, Byoung Ho;Choo, Hyo Jun;Kwon, Il;Choi, Eun Sik;Sohn, Sea Hwan
    • Korean Journal of Poultry Science
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    • v.46 no.3
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    • pp.193-204
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    • 2019
  • This study aimed to investigate the changes in production performance of five strains of Korean native chickens (KNCs), which have been collected and established at the National Institute of Animal Science, Korea, since 1992. A total of 38,026 KNCs were tested and survival rate, body weight, age at first egg-laying, hen-housed egg production, and egg weight was analyzed. The mean survival rates of KNCs were $87.9{\pm}0.8%$, but no significant difference in survival rate between strains and in the annual survival rates of KNCs was observed. The average body weight of KNCs was $1,609.7{\pm}21.3g$ at 150 d. The average body weight of KNC-Black was the highest, whereas KNC-White had the lowest weight. A gradual increase in the annual weight change has been observed in almost all strains after 2004. The average age at first egg-laying was $146.9{\pm}1.1d$ in KNCs where KNC-White was the earliest and KNC-Black was the latest. The age at first egg-laying has increased after 2003, but has gradually decreased after 2008. The average hen-housed egg production at 270 d was $77.3{\pm}1.7$ in KNCs, wherein KNC-Yellowish brown was the highest and KNC-Black was the lowest. The average egg weight at 270 d was $51.2{\pm}0.3g$ in KNCs, indicating that KNC-Black was the heaviest, whereas KNC-White was the lightest. A significant increase in annual egg weight has been observed since 2008. To conclude, the appearance and feather colors of KNCs have improved uniformly, and the body weight has also improved slightly since 2002.

Development of HRM Markers Based on SNPs Identified from Next Generation Resequencing of Susceptible and Resistant Parents to Gummy Stem Blight in Watermelon (수박에서 덩굴마름병 감수성 및 저항성 양친에 대한 차세대 염기서열 재분석으로 탐색된 SNP 기반 HRM 분자표지 개발)

  • Lee, Eun Su;Kim, Jinhee;Hong, Jong Pil;Kim, Do-Sun;Kim, Minkyong;Huh, Yun-Chan;Back, Chang-Gi;Lee, Jundae;Lee, Hye-Eun
    • Korean Journal of Breeding Science
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    • v.50 no.4
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    • pp.424-433
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    • 2018
  • Watermelon (Citrullus lanatus) is an economically important vegetable crop all over the world, which has functional compounds such as lycopene and citrulline. Gummy stem blight caused by Didymella bryoniae is one of the most devastative diseases in watermelon. Single nucleotide polymorphisms (SNPs), which are genetic variations occurring between individuals with respect to a single base, were often used to construct genetic linkage maps and develop molecular markers linked to a variety of horticultural traits and resistance to several diseases. In this study, we developed high-resolution melting (HRM) markers based on SNPs generated from NGS resequencing of two parents in watermelon. Plant materials were C. lanatus '920533' (female and susceptible parent), C. amarus 'PI 189225' (male and resistant parent), and their $F_1$ and $F_2$ progenies. A total of 13.6 Gbp ('920533') and 13.1 Gbp ('PI 189225') of genomic sequences were obtained using NGS analysis. A total of 6.09 million SNPs between '920533' and 'PI 189225' were detected, and 354,860 SNPs were identified as potential HRM primer sets. From these, a total of 330 primer sets for HRM analysis were designed. As a result, a total of 61 HRM markers that have polymorphic melting curves were developed. These HRM markers can be used for the construction of SNP-based linkage maps and for the analysis of quantitative trait loci (QTLs) related to gummy stem blight resistance.

Exploring the Objectives and Contents of Global Citizenship Education in the NSFCS 3.0: Focusing on the View of the 'World' and the Keywords (미국 국가 기준 가정과교육과정에 포함된 세계시민교육 관련 목표와 내용 탐색: '세계'관점과 핵심어를 중심으로)

  • Heo, Young-Sun;Kim, Nam-Eun;Chae, Jung Hyun
    • Journal of Korean Home Economics Education Association
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    • v.33 no.3
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    • pp.107-127
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    • 2021
  • The purpose of this study is to examine the relationship between the content areas and competencies of the Family & Consumer Sciences National Standards(NSFCS 3.0) of the U. S. and UNESCO Global Citizenship Education(GCED). For this purpose, the global perspective, content areas and competencies in NSFCS 3.0 and the keywords related to the three areas of content areas of UNESCO GCED were analyzed. Specifically, the content standards and competencies related to the words 'world' or 'global' were extracted and their relationship to the GCED topics and keywords were analyzed. The results of the study are as follows. First, NSFCS 3.0 described the direct correlation between individuals and the world by recognizing individuals as global citizens in 14 areas except for 'interpersonal relations' and 'parenting', specifically using the keyword of 'world' in content standards and competencies. Second, in the content standards and competencies of NSFCS 3.0, the keywords related to the topics of GCED areas were presented evenly in the three areas of FCS, dietary habits, family life, and human development. The social and emotional areas were not presented in clothing, housing, and consumer life. On the other hand, the behavioral area, which is emphasized most in the GCED, is presented in all the FCS content areas. From this, it is apparent that the learning field for GCED may be considered as the area of life pursued by the home economics curriculum. The results of this study provide foundational bases for understanding the relationship between NSFCS 3.0 and the GCED, and implications as to how to implement the content of the GCED in the next revision of the national home economics curriculum of Korea.

Analysis of socio-demographic and dietary factors associated with fruit and vegetable consumption among Korean adolescents: use of data from the 7th and 8th Korea National Health and Nutrition Examination Survey (2016-2019) (한국 청소년의 과일 및 채소 섭취와 관련된 인구사회학적 특성 및 식생활 분석: 국민건강영양조사 제7-8기 (2016-2019) 자료 이용)

  • Bokyeong Yun;Seunghee Kye
    • Journal of Nutrition and Health
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    • v.57 no.3
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    • pp.292-306
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    • 2024
  • Purpose: This study investigated fruit and vegetable intake and associated socio-demographic and dietary factors, and compared the nutritional intake according to the fruit and vegetable intake level among Korean adolescents. Methods: This study was conducted on 1,676 adolescents who participated in the 2016-2019 Korea National Health and Nutrition Examination Survey. The subjects were classified into four groups based on the fruit and vegetable intake recommendations in 2020 Dietary Reference Intakes for Koreans: Application (KDRIs Application): sufficient fruit intake (SF) group, sufficient vegetables intake (SV) group, sufficient fruit and vegetables intake (SFV) group, and not sufficient fruit and vegetable intake (NS) group The nutrient intake per day in each group was compared.. Logistic regression analysis was performed to examine the factors influencing fruit and vegetables intake. Results: In the sample of adolescents surveyed, only 1.40% met the recommended daily intake of fruits and vegetables, while 79.54% fell below the established threshold for adequate consumption. Female adolescents, those with fathers holding university degrees or above, and those who ate breakfast at least three times a week were likelier to have adequate fruit intake. Male adolescents and those from higher-income households were likelier to consume vegetables. Females, those who ate out daily, those from lower-income households, and those who understood food labels were likelier to have adequate fruit and vegetable intake. The daily nutrient intake and intake-to-requirement ratio significantly differed according to the fruit and vegetable intake groups. The NS and SF group had lower ratios for calcium and iron, while the NS group had the lowest vitamin A and C intake. By contrast, the SFV group met almost all daily nutrient requirements, except for calcium and vitamin A. Conclusion: This study highlights the need for nutrition education programs to encourage adolescents to consume adequate amounts of fruits and vegetables.