• The Korean Journal of Pesticide Science
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• v.9 no.1
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• pp.102-107
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• 2005

To select microorganisms that exhibit antifungal activity against the fungal pathogens of pepper, Phytophthora capsici and Colletotrichum acutatum, soil samples from a forest and natural fields of Gajang-Dong, Sangju-city were tested in vitro and in vivo. Streptomyces griseofuscus 200401 was finally selected throughout antifungal activity test with dual culture, culture broth, and fruits. For the identification of the strain, nucleotide sequences of 16S rDNA and whole cell fatty acids were analyzed. It is like that the strain 200401 may be a novel biological control agent that can reduce application of chemical fungicides to control late blight and anthracnose on pepper.

• Research in Plant Disease
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• v.29 no.3
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• pp.268-275
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• 2023

This study was conducted to confirm the utilization of Pleurospermum camtschaticum root extract as an organic agricultural material. Antioxidant activity of P. camtschaticum root extract, closely related to antibacterial activity, increased in a dose-dependent manner. In mycelial growth inhibitory activity, 100% P. camtschaticum root extract supressed over 70% for Colletotrichum coccodes and over 68% for Colletotrichum dematium. In the pepper fruit anthracnose development test, the size of the lesion decreased in a dose-dependent manner, which showed the same tendency as the previous results in inhibitory activity on mycelial growth. In the pepper seed germination and red pepper growth promotion test of P. camtschaticum root extract, oposite results was confirmed. The lower the concentration, the more the seed germination and growth promotion effects were shown. The phenol content of pepper leaves was also measured after pepper growth promotion test have been completed. The phenol content related to antibacterial activity increased in all treated groups compared to the untreated group. Therefore, the results of this study showed the possibility of development as an organic material.

• Korean Journal of Microbiology
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• v.37 no.1
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• pp.21-27
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• 2001

Recently many investigators have devoted considerable attention to the production and purification of PA for antigens, and the preparation of new synthetic medium (RM medium) have solved to increase the yields of the PA but, the low sensitivity of the PA to detect B. anthracis infections has remained as a problem to be solved. This study was undertaken to evaluate the yields of the PA from culture filtrates of B. anthracis Sterne $34F_2$ strain in modified RM medium in which 10 g/l of $NaHCO_3$ and 10g/l of glucose were replaced by 8 g/l and 5 g/l, and the first purification step of PA from culture broth was used hydroxyapatite. The PA was purified by hydroxyapatite column chromatography, DEAE-Sepharose CL-4B column chromatography and Toyo-pearl gel filtration chromatography. The yield of PA from the modified RM medium, 8.6 mg/l.

• The Korean Journal of Mycology
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• v.29 no.1
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• pp.9-14
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• 2001

Colletotrichum species are important fungal pathogens that cause great damages on various host plant species worldwide. In Korea, Colletotrichum species cause massive economic losses on apple, peach, grape, and especially, sweet persimon productions. In the past, identification of the pathogen and the studies on the genetic relationships among the pathogenic isolates were mainly based on morphology, cultural characteristics, and the difference in pathogenicity. However, in recent years, these traditional methods have been replaced with molecular methods including AFLP. AFLP method with the merits of both RAPD and RFLP has been widely used for the genetic relationship studies of various organisms. Therefore, in this study, AFLP method was employed for the studies of genetic relationships among the different isolates of Colletotrichum species collected from various parts of sothern Korea. As a result, two specific band pattern groups were observed among different isolates of Colletotrichum species.

• The Bimonthly Magazine for Agrochemicals and Plant Protection
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• v.7 no.3
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• pp.92-96
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• 1986

고추에 피해를 주고 있는 병으로 잎오갈병, 더뎅이병, 탄저병, 역병등 19종이 우리나라에 보고 되어 있다. 이러한 고추의 병해를 병원균별로 보면 담배모자이크바이러스등 바이러스가 5종, 더뎅이병균등 세균이 3종이 있으며 곰팡이는 역병균등 14종이 고추에 병을 일으키고 있다. 이와같은 고추병들이 고추에 피해를 주고 있지만 그중에서도 특히 고추를 이어짓기 하는 밭이나 비닐하우스에서 고추의 작황을 좌우하는 것은 대부분이 역병에 의한 피해이다. 역병의 피해는 물론 고추를 이어짓기함으로써 토양 전염되는 역병균의 전염원이 매년 증가하기 때문이기도 하지만 역병이 발생하기 쉬운 여름철에 장마가 겹치므로 물빠짐이 나빠지거나 침수가 되기 때문에 피해가 증가하는 요인이 되고 있다.

• Proceedings of the Korea Air Pollution Research Association Conference
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• 2002.04a
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• pp.97-98
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• 2002

최근 들어 구제역과 같은 세균에 대한 경계와 관심이 늘어가고 있다. 하지만, 관심에 비하여 이에 대한 연구가 부족한 실정이고 대기질과 상호 연계한 연구는 더욱 미미한 상태이다. 따라서, 본 연구에서는 광주광역시의 시간대별 교통량과 대기질 그리고 낙하세균의 수를 함께 조사하여 상호연관성을 찾아보고자 하였다. 낙하세균은 탄저균이나 구제역처럼 전염성이나 인체에 미치는 영향은 크지 않지만 공중보건과 대기질 관리의 관점에서 이에 대한 정보 확보와 관련대책이 시급하다. (중략)

• Journal of Environmental Health Sciences
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• v.33 no.5
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• pp.434-440
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• 2007

The livestocks are sometimes infected with pathogenic microorganisms such as bird influenza, brucellosis, pig cholerae, and salmonella. However, it is difficult to predict the outcome of these diseases because the livestocks are mostly raised in the rural areas. Efficient systems for detecting and alerting the onset of livestock diseases are urgently required. In these studies, the fluorescent analysis method, luminescent analysis method, and frequently used gene amplification method (polymerase chain reaction) have been developed in order to detect the pathogenic microbes in the early stages of disease progression. By using these developed systems, damages due to the livestock diseases induced by microbes can be minimized. If we can detect livestock diseases in the early stage, the costs for diagnosis and treatment will be reduced, and the livestock can be quickly recovered.

• Journal of the Korea Institute of Military Science and Technology
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• v.18 no.4
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• pp.478-483
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• 2015

Decontamination of biological agents utilizes hydrogen peroxide($H_2O_2$) for its effectiveness and safeness. Bacillus anthracis is a major target for $H_2O_2$ decontamination. To assess the effect of $H_2O_2$ on B. anthracis and identify biomarkers for decontamination, whole transcriptomic profiling of $H_2O_2$-treated B. anthracis was performed. Here we identified deregulation in stress response genes, transcription factors and cellular homeostasis genes. We also found that expression of antisense RNAs increased in B. anthracis during decontamination. We postulate that B. anthracis prioritizes survival and adaptation in response to $H_2O_2$ treatment by changing its gene expression pattern.

• Journal of the Korea Institute of Military Science and Technology
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• v.14 no.4
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• pp.710-718
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• 2011

Edema factor(EF) is a portion of anthrax toxin which produces edema when combined with protective antigen. This paper describes about technique for cloning, expression, purification and activity test of EF. Using the E. coli expression system, we could make recombinant EF protein although it's origin is Bacillus anthracis. And also we could culture massively and purify highly pure protein. Finally we confirm a enzyme activity of purified EF to increase intracellular cAMP level. Through establishing this technique, it can be possible to research about EF in depth and apply to expression and purification of many other protein in biology.

• Journal of Life Science
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• v.16 no.3 s.76
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• pp.420-426
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• 2006

Bacillus sp. KMU-991 was isolated from Oslo city soils at Norway and shown a strong antifungal activity on red-pepper anthracnose, Colletotrichum gloeosporioides. Bacillus sp. KMU-991 produced a maximum level of antifungal substrate under aerobic incubation at $30^{\circ}C$, 180 rpm for 48 hours in TSB medium(initial pH 7.0) containing 1.0% mannitol and 1.0% ammonium chloride. Precipitate of culture broth by $30{\sim}60%$ ammonium sulfate precipitation exhibited strong antifungal activity against C. gloeosporioides KACC 40804. Butanol extract of cultured broth also shown fungal growth inhibitory activity against Fusarium oxysporum f. sp. radicus-lycopersici KACC 40537, Rhizoctonia solani AG-4 KACC 40142, Botrytis cinerea KACC 40573, Colletotrichum orbiculare KACC 40808, and Phytophthora cambivora KACC 40160 by agar diffusion method.