• Korean Journal of Microbiology
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• v.37 no.1
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• pp.49-55
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• 2001

Bacillus anthracis is a gram-positive spore-forming bacterium that causes the disease anthrax. The anthrax toxin contains three components, including the protective antigen (PA), which binds to eucaryotic cell surface receptors and mediates the transport of toxins into the cell. In this study, the entire 2,294-nucleotide protective antigen gene (pag) was sequenced from 4 of B. anthracis strains to identify potential variation in the toxin and to further our understanding of B. anthracis evolution in Korea. Sequence alignment of the entire PA gene from 30 strains representative of the four B. anthracis diversity groups revealed mutations. The mutation of B. anthracis BAK are located adjacent to a highly antigenic region crossing the junction between PA domains 3 and 4 shown to be critical to LF binding. The different mutational combinations observed in this study give rise to 11 PA genotypes and 4PA phenotypes. Three-dimensional analysis of all the amino acid changes (Ala to Val) observed in BAK indicated that these changes are not only close sequentially but also very close in three-dimensional space to the antigenic region importan tfor LF binding. Phylogenetic (cladistic) analysis of the pag corresponded with previous strain grouping based on chromosomal variation, suggesting that plasmid evolution in B. anthracis has occurred with little or no horizontal transfer between the different strains.

• Korean Journal of Microbiology
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• v.39 no.1
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• pp.40-44
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• 2003

Bacillus anthracis is a gram-positive spore-forming bacterium that causes the disease anthrax. In order to develop a DNA marker specific for Bacillus anthracis and to discriminate this species from Bacillus cereus group, we applied the randomly amplified polymorphic DNA (RAPD)-PCR technique to a collection of 29 strains of the genus Bacillus, including 22 species of the B. cereus group. A 709-bp RAPD marker (KHT5) specific for B. anthracis was obtained from B. anthracis BAK. The PCR product of internal primer set from the KHT5 fragment distinguished B. anthracis from the other species of the B. cereus group.

• Korean Journal of Microbiology
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• v.37 no.1
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• pp.56-60
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• 2001

Molecular typing of Bacillus anthracis has been extremely difficult due to the lack of polymorphic DNA markers. Aiming to develop a DNA marker specific for Bacillus anthracis and to be able to discriminate this species from Bacillus genus, we applied the random amplified polymorphic DNA (RAPD)-PCR. We have identified B. anthracis from various Bacillus species. The analysis performed by RAPD clearly demonstrated substantial genetic variations among Bacillus species. This type of analysis is an easy, quick and highly discriminatory technique that may help in diagnosis of anthrax.

• Korean Journal of Microbiology
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• v.46 no.1
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• pp.21-26
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• 2010

As the causative agent of Anthrax, Bacillus anthracis causes an acute fatal disease in herbivores such as cattle, sheep, and horses as well as humans. The therapeutics and prevention of anthrax currently available are based on antibiotics and the live attenuated vaccine strains, which may be problematic due to the emergency of antibiotic resistant strains or residual virulence in those vaccine strains. Therefore, it has been required to develop novel therapeutics and vaccines which are safer and applicable to humans. Recently, the development of the multivalent vaccine targeting both spores and vegetative cells of B. anthracis along with anthrax toxin has been reported. In our attempts to screen potential candidates for those multivalent vaccines, the whole genomic expression library of B. anthracis was constructed in this study. To the end, the partial digests of the genomic DNA from B. anthracis (ATCC 14578) with Sau3AI were ligated with the inducible pET30abc expression vectors, resulting in approximately $1{\times}10^5$ clones in E. coli BL21(DE3). The redundancy test by DNA nucleotide sequencing was performed for the randomly selected 111 clones and found 56 (50.5%) B. anthracis genes, 17 (15.3%) vector sequences, and 38 (34.2%) unknown genes with no sequence homology by BLAST. An inducible expression of the recombinant proteins was confirmed by Western blot. Interestingly, some clones could react with the antiserum against B. anthracis. These results imply that the whole genomic library constructed in this study can be applied for analyzing the immunomes of B. anthracis.

• Journal of Korea Society of Industrial Information Systems
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• v.25 no.5
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• pp.73-81
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• 2020

One of the most important vegetables consumed in Korea, chili peppers (Capsicum annuum) are widely cultivated around the world. Chili peppers have been severely damaged by anthracnose during their growth, so it is important to study prevention and resistance varieties. K1 anthracnose was inoculated against four cultivar of chili peppers that are resistant to anthracnose and one cultivar that is sensitive. The area of the disease that appeared over time was photographed and quantified through the program was analyzed. Through the ratio of the area of chili pepper fruit and the area of the bottle, the sensitive variety An-S showed weak reactions to anthracnose with about 40%, the resistant variety An-12R (23%), An-Tan (21%), and An-9R (19%), and PBC81 showed a strong response to anthracnose with about 11%. These quantitative value can be used as a basis for comparison in conducting resistance studies for new varieties.

• 동위원소뉴스
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• no.10 s.58
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• pp.6-7
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• 2001

• Korean Journal Plant Pathology
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• v.14 no.5
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• pp.543-544
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• 1998

An anthracnose on potato leaves was observed in Kangwon alpine and Kangnung areas in Korea. A fungal pathogen was repeatedly isolated from the leaf lesions and stems of the infected plants and identified as Colletotrichum coccodes. The fungus showed pathogenicity on the leaves of potato inoculated. This is the first report that anthracnose of potato caused by Colletotrichum coccodes was occurred in Korea.

• Journal of the Korea Institute of Military Science and Technology
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• v.20 no.5
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• pp.726-732
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• 2017

Bacillus anthracis, Vibrio cholerae, Variola virus and Shigella dysenteriae are classified as category A and B biological weapons. In this study suggest that 4 genes of Bacillus anthracis, 2 genes of Vibrio cholerae, 1 gene of Variola virus and 1 gene of Shigella dysenteriae were detective 50~500 fg of target DNA per reaction using real-time PCR based assay. Also analytical specificity did not show any cross-reactivity with other related bacteria. Reliable and one reaction could be effective early diagnostic and treatment for detection of unknown samples.

• Proceedings of the Korean Society of Veterinary Service Conference
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• 1995.04a
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• pp.91.1-91
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• 1995

• The Korean Journal of Mycology
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• v.15 no.2
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• pp.116-117
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• 1987

1985년 9월, 경기지방의 꽃 재배온실에서 네오레게리아(Neoregelia carolinae Smith var, tricolor Hort.)에 탄저병징이 심하게 발생하여, 병반에서 병원균을 분리, 동정한 결과 Colletotrichum gloeosporioides Penz.에 의한 것으로 밝혀졌다. 이 균은 PDA배양에서 완전세대 Glomerella cingulata (Stonem.) Spauld. & v. Sch.의 자낭과 자낭포자를 형상하였다. 병징은 2-5mm 크기로 원형 내지 타원형의 적갈색 반점이며, 반점 주위는 황색을 띄었다. 병이 심하게 진전되면 잎의 대부분이 황갈색으로 변하여 말라 죽었다. 이 균의 분생포자현탁액을 네오레게리아에 분무접종한 결과 병원성이 확인되었다. C. gloeosporioides에 의한 네오레게리아 탄저병은 아직까지 보고된 바 없으므로 새로운 탄저병으로 보고한다.