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Discrimination of Bacillus anthracis from Bacillus cereus Group Using KHT5 Marker  

김형태 (한양대학교 생화학 및 분자생물학과)
김성주 (한양대학교 생화학 및 분자생물학과)
채영규 (한양대학교 생화학 및 분자생물학과)
Publication Information
Korean Journal of Microbiology / v.39, no.1, 2003 , pp. 40-44 More about this Journal
Abstract
Bacillus anthracis is a gram-positive spore-forming bacterium that causes the disease anthrax. In order to develop a DNA marker specific for Bacillus anthracis and to discriminate this species from Bacillus cereus group, we applied the randomly amplified polymorphic DNA (RAPD)-PCR technique to a collection of 29 strains of the genus Bacillus, including 22 species of the B. cereus group. A 709-bp RAPD marker (KHT5) specific for B. anthracis was obtained from B. anthracis BAK. The PCR product of internal primer set from the KHT5 fragment distinguished B. anthracis from the other species of the B. cereus group.
Keywords
Bacillus anthracis; Bacillus cereus group; identification; Bacillus anthracis specific marker; RAPD-PCR;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
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