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http://dx.doi.org/10.9766/KIMST.2017.20.5.726

A Study on the Validation system of Detection for Biological Agents Using Real-Time PCR  

Cha, Younggil (Molecular Diagnostic Division, Bioneer Corp.)
Koo, Bonwoo (Molecular Diagnostic Division, Bioneer Corp.)
Kim, Seongjoo (The 5th Research and Development Institute, Agency for Defense Development)
Kim, Namil (Molecular Diagnostic Division, Bioneer Corp.)
Park, Hanoh (Molecular Diagnostic Division, Bioneer Corp.)
Publication Information
Journal of the Korea Institute of Military Science and Technology / v.20, no.5, 2017 , pp. 726-732 More about this Journal
Abstract
Bacillus anthracis, Vibrio cholerae, Variola virus and Shigella dysenteriae are classified as category A and B biological weapons. In this study suggest that 4 genes of Bacillus anthracis, 2 genes of Vibrio cholerae, 1 gene of Variola virus and 1 gene of Shigella dysenteriae were detective 50~500 fg of target DNA per reaction using real-time PCR based assay. Also analytical specificity did not show any cross-reactivity with other related bacteria. Reliable and one reaction could be effective early diagnostic and treatment for detection of unknown samples.
Keywords
Bacillus Anthracis; Vibrio Cholerae; Shigella Dysenteriae; Variola Virus; Real-Time PCR; Bacillus Anthracis; Vibrio Cholerae;
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