• KSBB Journal
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• v.9 no.2
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• pp.140-146
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• 1994

Aureobasidium pullulans produced high concentration of polyols extracellularly in the media of sucrose, glucose and mannose as sole carbon source. Mannitol was the main polyol produced during the late exponential and stationary phases of growth together with small quantities of glycerol. Sucrose and glucose were rather rapidly metabolized to mannitol among carbon sources examined where the initial glucose concentration showed no difference in the amount of mannitol. In contrast 20%(w/v) of sucrose was the most appropriate concentration tested. However, the yield of mannitol based on substrate used($Y_{p/s}$) was independent on the initial concentration, and the mean value of mannitol yield in 10% glucose and sucrose media was 0.144 and 0.188, respectively. Mannitol production was reduced in response to an elevated water stress imposed by salts within the range from 0.25 to IM of NaCl or KCl as stress solutes. However, glycerol contents and its ratio to mannitol were increased at the conditions of high salinity. Based on the results, extracellular mannitol produced by A. pullulans probably resulted partly from osmoregulation(in case of glycerol) and mainly from, as known to occur in most of fungi, enzymatic reduction of the corresponding hexoses through phosphate pathway.

• Proceedings of the Korea Water Resources Association Conference
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• 2022.05a
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• pp.82-82
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• 2022

최근 지구온난화로 인한 전 세계적 기후변화가 일어나고 있으며, 이러한 지구온난화 방지 대책으로 탄소의 중요성과 탄소중립을 선언하는 국가가 증가하고 있다. 탄소의 중요성이 증가함에 따라 유역 내의 탄소 중립이 중요 이슈로 떠오르고 있다. 유역 내 탄소 저장원으로는 숲, 하천, 토양 등이 존재하나 하천의 경우 탄소의 저장이 곧 수질 오염과 연결이 되기에 바람직한 방안이 될 수 없다. 그러나 토양의 경우 방대한 양의 탄소를 저장하기에 적합한 기능을 하기에 다른 저장원들에 비해 중요한 저장원으로 대두되고 있다. 토양탄소의 경우 일반적으로 유기물과 무기물의 형태로 토양에 저장된다. 이중 토양유기탄소는 장기간 토양 속에서 대기와의 탄소 조절 역할을 하기에 중요 요인으로 대두되고 있다. 하지만 기후변화로 인한 국지성 호우 및 무분별한 개발 등이 증가함에 따라 유역 내 토양 생태계의 변화가 일어나고 있으며, 이에 따른 유역 내 토양유기탄소 또한 손실이 일어나고 있다. 따라서 본 연구에서는 토양의 특성과 모델을 활용하여 유역단위 토양유기탄소량의 변화량을 산정하여 비교 및 분석을 하고자 한다. 이를 위해서 토양유기탄소의 모의가 가능한 APEX 모델을 활용하였으며, 선정된 연구 대상 지역의 토양 특성 자료를 활용하여 입력자료 전처리를 진행 후 모의를 진행하였다. 이후 선행연구 및 보고서를 통한 실측자료를 기반으로 모델 매개변수 보정을 진행하였으며, 보정된 결과를 통해 유역에 대한 토양유기탄소를 산정을 진행하였고 기간별 변화의 차이를 분석하였다. 해당 연구를 통해 유역 내 잠재되어있는 토양유기탄소량 정량화 등의 연구에 활용될 수 있을 것으로 기대한다.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2002.11b
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• pp.61-61
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• 2002

This study was excuted to decide the physiological characteristics of Kuehneromyces mutabilis. K. mutabilis was tested to select pertinent substract, temperature and pH range for the growth. Mycelial growth of K. mutabilis was mostly supported on MYPA among other tested synthetic or semi-synthetic media. The temperature range for pertinent mycelial growth was about 25~32$^{\circ}C$ and mostly stimulated at $25^{\circ}C$. And the pertinent pH range of MYPA was 5.0~6.0. The required carbon and nitrogen source for mycelial growth of K. mutabilis was tested. The mycelial growth was mostly stimulated by soluble starch at content. The carbon sources for pertinent mycelial growth was mannose or glucose. And the nitrogen source for pertinent mycelial growth was yeast extract or peptone.

• Microbiology and Biotechnology Letters
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• v.31 no.1
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• pp.57-62
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• 2003

A bacteria strain producing extracellular $\beta$-mannanase was isolated from soil and was identified as Bacillus subtilis by 16S rRNA sequence comparison and biochemical determinations. The optimum pH and temperature for the $\beta$-mannanase activity were 5.0 and 5.5$^{\circ}C$, respectively. The zymogram technique revealed a single protein band exhibiting $\beta$-mannanase activity from the culture supernatant. The molecular mass of the enzyme was estimated at approximately 130 kDa. The addition of 0.5% lactose or 0.5% locust bean gum to the LB medium caused to Increase significantly the $\beta$-mannanase productivity from Bacillus subtilis JS-1. The cells grown on LB medium supplemented with lactose produced maximal enzyme activity at the stationary phase. In contrast to this, the $\beta$-mannanase was induced at the logarithmic phase from the cells grown on LB medium supplemented with locust bean gum. The discrepancy in induction times suggests that $\beta$-mannanase was induced by different induction mechanisms depending on the carbon sources in Bacillus subtilis JS-1 .

• Journal of Korean Society of Environmental Engineers
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• v.22 no.7
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• pp.1243-1252
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• 2000

The differential enhanced degradation of cis- and trans-1,3-D was observed in the previous two studies performed by several researchers. This study was initiated to investigate the involvement of microorganisms in the differential enhanced degradation of the chemicals. As expected, microorganisms were responsible for the enhanced degradation. A mixed bacterial culture capable of degrading 1,3-D was isolated from an enhanced soil sample collected from a site treated with 1,3-D. Similar to the enhanced soil, the mixed culture degraded trans-1,3-D faster than cis-1,3-D. This mixed culture could not utilize cis- and trans-1,3-D as a sole source of carbon for growth. Rather, a variety of second substrates were evaluated to stimulate the differential enhanced degradation of the two isomers. As a result, the mixed culture degraded cis- and trans-1,3-D only in the presence of a suitable second substrate. Therefore, it appeared that the degradation of cis- and trans-1,3-D was a cometabolic process. Second substrates that had the capacity to stimulate the degradation included soil leachate, tryptone, tryptophan, and alanine. Other substrates tested. including soil extract. glucose, yeast extract and indole, failed to stimulate the degradation of the two isomers. The mixed culture was composed of four morphologically distinctive colonies on L-agar plates.

• Journal of Life Science
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• v.14 no.5
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• pp.834-839
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• 2004

To improve and oil degrading activity, the lipase gene from Pseudomonase sp. was transformed into Klebsiella sp. KCL-l, an oil degrading bacterium. The selected trasformant was named as a KCL-1/pET-Lip. The surface tension of culture broth of KCL-1/pET-Lip was decreased to 33 dyne/cm from 55 dyne/cm using 4% (v/v) soybean oil as sole carbon source. The surface tension were 44 and 37.5 dyne/cm, to 2% (w/v) glucose and 4% (v/v) kerosene medium, respectively. The emulsification activity of the biosurfactant solution containing lipase of KCL-l/pET-Lip improved better than wild type KCL-l. The soybean oil was most efficient carbon source and substrate for surface activity and emulsification activity of KCL-1/pET-Lip. The expression of lipase was confirmed by SDS-PAGE.

• Journal of Life Science
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• v.17 no.4 s.84
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• pp.568-574
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• 2007

Lignin degrading bacterium Pseudomonas sp. LG2 was able to degrade lignin substrate to a lot of APPL compound. APPL compound was detected in culture supernatants from Pseudomonas sp. LG2 grown with BSC(brewer's spent grain). FAE(ferulic acid esterase) and xylanase are induced from Pseudomonas sp. LG2 in the presence of carbon sources such as oat spelt xylan, HBSG I, II(hydrolyzed brewer's spent grain I, II) and AFBSG(autoclaved fraction from brewer's spent grain). However, xylanase and FAE are not induced by growth of Pseudomonas sp. LG2 on xylose and arabinose. Pseudomonas sp. LG2 is grown on medium containing oat spelt xylan, HBSG I, II and AFBSG and the induction of FAE and xylanase activities of extracellular proteins determined during 14 days. Maximum level of xylanase activity(5.3 U/mg) found at 6 days in culture contained oat spelt xylan as carbon source, whereas maximum level of FAE activity(15.4 mU/mg) was found at 8 days in culture contained AFBSG as carbon source. Most ferulic acid was released in culture supernatants when Pseudomonas sp. LG2 grown on oat spelt xylan, HBSG I, II and AFBSG. FAE of extracellular enzymes was also specific activity on methyl ferulic acid, methyl caffeic acid and methyl p-coumaric acid respectively, but not methyl sinapinic acid, methyl vanillic acid and methyl gallic acid.

• Proceedings of the Korean Institute of Surface Engineering Conference
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• 2002.05a
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• pp.50-50
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• 2002

탄소 박막은 내마모, 화학적 생물학적 적합성 둥의 특성으로 언하여 많은 연구자들레 의하 여 연구되어지고 있다. 그러나 탄소 박막의 경우 높은 잔류응력과 이에 따른 낮은 밀착력으 로 언하여 증착 두께에 제한이 되어왔다. EB-PVD법을 이용하여 탄소막을 증착하는 경우 이러한 문제점을 해결하기 위하여 탄소막에 제 2의 금속원소를 첨가하거나 화합물 형태의 증착원 이용, 복합공정 (Hybrid process)을 통하여 증착 두께 의 제 한을 극복하고자 하는 연 구가 계속되고 있다. 본 연구에서는 탄소막의 잔류응력 제어와 증착 두께를 높이기 위하여 Plasma Activated E E-Beam Evaporation을 통하여 탄소막을 증착하였다. 탄소막 증착시 잔류응려과 밀착력에 대한 플라즈마의 영향올 알아보기 위하여 RF 플라즈마의 인업 전력에 따라 실험을 수행하였으며 필라멘트 전자 방출원을 이용하여 플라즈마 밀도가 미치는 영향도 알아보았다.

• 한국생물공학회:학술대회논문집
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• 2002.04a
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• pp.247-250
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• 2002

Pichia pastoris, a methylotrophic yeast widely used for the production of heterologous proteins, was used to produce Hepatitis B surface antigen (HBsAg) under the control of the strong, tightly-regulated alcohol oxidase promoter. It is highly induced during the growth on methanol, but the presence of non-methanol carbon sources such as glycerol and glucose repressed fully the expression of alcohol oxidase. In this study, glycerol and sorbitol feedings for the expression of the recombinant HBsAg were compared to examine the potential of sorbitol as a less repressive carbon source in fed-batch fermentation. The sorbitol feeding enhanced the production yield by 12% compared to that in glycerol feeding, although the cell concentration was lower.

• Journal of Environmental Impact Assessment
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• v.32 no.1
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• pp.41-48
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• 2023

This study was conducted to confirm the possibility of a new carbon stock in the Sejong National Arboretum, a major urban greenspace in Sejong-si. This study involved field and ground surveys of 1,336 trees, including 794 Pinus densiflora trees with a diameter at breast height (DBH) of above 5.5cm, which are the most planted in the Sejong National Arboretum, Chionanthus retusus 154 trees planted, Metasequoia glyptostroboides 216 trees, and Aesculus turbinata 172 trees as street trees. Measurements were performed from April to November. Based on the results of the survey, the carbon storage and annual carbon stock were calculated using the annual carbon stock estimation equation used in the forest carbon offset projects. As a result of comparing the carbon stock of the 12cm diameter class, which is the most distributed of four major trees, it was found in the order of C. retusus (0.0136tC/tree), P. densiflora (0.0126tC/tree), M. glyptostroboides (0.0092tC/tree), and A. turbinata (0.0076tC/tree). In addition, the field survey measurement data compared with terrestrial LiDAR measurement data for 20 trees showed a difference of 10.0cm in tree height and 1.7cm in diameter at breast height (p<0.05). In the future, additional carbon stock and annual uptake of other species planted in the arboretum are expected to promote the carbon uptake effect of the arboretum and contribute to the achievement of the national NDC. In the long term, it is also necessary to develop the carbon uptake factor of trees and shrubs mainly used to calculate the exact carbon uptake amount of trees mainly used in urban forests and gardens.