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http://dx.doi.org/10.5352/JLS.2007.17.4.568

Partial Characterization and Induction of Ferulic Acid Esterase and Xylanase from Pseudomonas sp. LG2  

Kim, Yong-Gyun (Dept. of Life Science & Environmental Biochemistry, College of Natural Resources & Life Science, Pusan National University)
Lee, Sang-Mong (Dept. of Life Science & Environmental Biochemistry, College of Natural Resources & Life Science, Pusan National University)
Park, Hyun-Chul (Dept. of Life Science & Environmental Biochemistry, College of Natural Resources & Life Science, Pusan National University)
Kim, Keun-Ki (Dept. of Life Science & Environmental Biochemistry, College of Natural Resources & Life Science, Pusan National University)
Son, Hong-Joo (Dept. of Life Science & Environmental Biochemistry, College of Natural Resources & Life Science, Pusan National University)
Publication Information
Journal of Life Science / v.17, no.4, 2007 , pp. 568-574 More about this Journal
Abstract
Lignin degrading bacterium Pseudomonas sp. LG2 was able to degrade lignin substrate to a lot of APPL compound. APPL compound was detected in culture supernatants from Pseudomonas sp. LG2 grown with BSC(brewer's spent grain). FAE(ferulic acid esterase) and xylanase are induced from Pseudomonas sp. LG2 in the presence of carbon sources such as oat spelt xylan, HBSG I, II(hydrolyzed brewer's spent grain I, II) and AFBSG(autoclaved fraction from brewer's spent grain). However, xylanase and FAE are not induced by growth of Pseudomonas sp. LG2 on xylose and arabinose. Pseudomonas sp. LG2 is grown on medium containing oat spelt xylan, HBSG I, II and AFBSG and the induction of FAE and xylanase activities of extracellular proteins determined during 14 days. Maximum level of xylanase activity(5.3 U/mg) found at 6 days in culture contained oat spelt xylan as carbon source, whereas maximum level of FAE activity(15.4 mU/mg) was found at 8 days in culture contained AFBSG as carbon source. Most ferulic acid was released in culture supernatants when Pseudomonas sp. LG2 grown on oat spelt xylan, HBSG I, II and AFBSG. FAE of extracellular enzymes was also specific activity on methyl ferulic acid, methyl caffeic acid and methyl p-coumaric acid respectively, but not methyl sinapinic acid, methyl vanillic acid and methyl gallic acid.
Keywords
Pseudomonas sp. LG2; ferulic acid esterase; xylanase;
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