• Journal of Plant Biotechnology
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• v.38 no.2
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• pp.176-185
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• 2011

Rice is one of the most important crop in the world, in particular for food resources. With its small genome size of 383 Mb, the Oryza sativa is a model plant for genome research. Indeed, it's grain provides human with a source of carbohydrates and proteins. Rice grain has relatively low protein contents (around 8%) compared to other legume seeds (around 40%). Osborne classified seed proteins into water soluble albumin, salt soluble globulin, alcohol soluble prolamin and acidic/alkaline solution soluble glutelin. Glutelin and prolamin are the major storage proteins in rice. For the gene expression study of seed storage proteins, we analyzed 33,192 EST clones at immature stages in a rice cultivar (Oryza sativa L. cv. 'Ilpum'). Based on the expression analysis, we cloned 11 glutelin genes and figured out the 8 genes are located on Chromosome 2. The expression of glutelin genes appears to be about 28.2% of total level in immature seeds. Interestingly, glu-04 is duplicated as inverted sequences on the same chromosomes as far 4.5 kb. Our results indicate that glutelin genes, evolutionarily, were replicated on the chromosome and thus expressed as specific manners. In a whole protein composition analysis, glu05 (type B7) contains the highest lysin contents (4.51%) among the 11 rice glutelin genes. It will be an interesting future work to increase lysin contents by the gene overexpressor strategy with the aim of improved diet nutritionally fortified.

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.29 no.5
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• pp.217-227
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• 2017

The object of this study is to estimate the net volume transport and the residual flow that changed by space and time at southern part of Yeomha channel, Gyeonggi Bay. The cross-section observation was conducted at the mid-part (Line2) and the southern end (Line1) of Yeomha channel for 13 hours during neap and spring-tides, respectively. The Lagrange flux is calculated as the sum of Eulerian flux and Stokes drift, and the residual flow is calculated by using least square method. It is necessary to unify the spatial area of the observed cross-section and average time during the tidal cycle. In order to unify the cross-sectional area containing such a large vertical tidal variation, it was necessary to convert into sigma coordinate system by horizontally and vertically for every hour. The converted sigma coordinate system is estimated to be 3~5% error when compared with the z-level coordinate system which shows that there is no problem for analyzing the data. As a result, the cross-sectional residual flow shows a southward flow pattern in both spring and neap tides at Line2, and also have characteristic of the spatial residual flow fluctuation: it northwards in the main line direction and southwards at the end of both side of the waterway. It was confirmed that the residual flow characteristics at Line2 were changed by the net pressure due to the sea level difference. The analysis of the net volume transport showed that it tends to southwards at $576m^3s^{-1}$, $67m^3s^{-1}$ in each spring tide and neap tide at Line2. On the other hand, in the control Line1, it has tendency to northwards at $359m^3s^{-1}$ and $248m^3s^{-1}$. Based on the difference between the two observation lines, it is estimated that net volume transport will be out flow about $935m^3s^{-1}$ at spring tide stage and about $315m^3s^{-1}$ at neap tide stage as the intertidal zone between Yeongjong Island and Ganghwa Island. In other words, the difference of pressure gradient and Stokes drift during spring and neap tide is main causes of variation for residual current and net volume transport.

• Journal of Environmental Impact Assessment
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• v.26 no.6
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• pp.404-417
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• 2017

In this study, we analyzed response patterns of rotifer community to eutrophic state, and estimated the applicability of rotifer community as an environmental indicator for highly eutrophicated reservoirs. In order to evaluate the relationships among spatial and temporal distributions and the water quality of rotifer community, we selected the Jundae Reservoir and Chodae Reservoir in Chungcheongnam-do, Korea, which are geographically adjacent but have different water quality, particularly in their eutrophic states. For the analyses on their correlations, monthly survey of water quality and rotifer community, was conducted from April to November 2013 in both reservoirs. The rotifer community was divided into different compositions of functional groups as well as species. Functional groups were classified according to the structure and shape of trophi which can represent feeding behavior of rotifer genus. To reflect ecological characteristics of species, body size and habitat preferences were also considered. Species-based composition did not show a consistent tendency with water quality parameters related with eutrophication. On the contrary, functional group composition showed relatively clear group-specific patterns, increasing or decreasing according to the parameters. The results suggest the possible application of rotifer functional group composition as an indicatorforthe lentic systems, especially hyper-eutrophicated reservoirs. The present study can suggest the applicability based on the field observations from the limited time scale and sites, and further studies on feeding behavior of the rotifer functional group and its interactions with environmental variables are necessary for the further application.

• Applied Microscopy
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• v.35 no.3
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• pp.165-176
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• 2005

Studies on molecular plasticity of Bermann glia (BG) after harmaline-induced Purkinje cell (PC) degeneration in the rat cerebellum. The intimate structural relationship between BG and PC, evidenced by the sheathing of the PC dendrites by veil-like process from the BG has been suggestive of the close functional relationship between these two cell types. However, little is known about metabolic couplings between these cells. This study designed to investigate molecular plasticity of BG in the rat cerebellum in which PCs were chemically ablated by harmaline treatment. Immunohistochemical examination reveals that harmaline induced PC degeneration causes a marked glial reaction in the cerebellum with activated BG and microglia aligned in parasagittal stripes within the vermis. In these strips, activated BG were associated with upregulaion of metallotheionein, while GLAST and was down regulated, as compared with nearby intact area where both BG are in contact with PCs. The data from this study demonstrate that BG can change their phenotypic expression when BG loose their contact with PCs. It is conceivable that activated BG may upregulate structural proteins, metallothionein expression to use for their proliferation and hypertrophy; metallothionein expression to cope with oxidative stress induced by PC degeneration and microglial activation. On the contrary, BG may down regulated expression of GLAST because sustained loss of contact with PCs would eliminate the necessity for the cellular machinery involved glutamate metabolism. In conclusion, BG might respond man to death of PCs by undergoing a change in metabolic state. It seems possible that signaling molecules released from PCs regulates the phenotype expression of BG. Also ultrastructures in the organelles of normal PC and BG are distinguished by mitochondrial appearance, and distributed vesicles at the synaptic area in the cytoplasm.

• Applied Microscopy
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• v.38 no.2
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• pp.117-124
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• 2008

Salvinia is an aquatic plant forming dimorphic leaves that have been modified into floating and submerged leaves. A air of floating leaves plays an important role for the floating and photosynthesis while the submerged leaves, which are lim and long, have the form and function of root. Many aquatic plants develop trichomes in the epidermis but in Salvinia, richomes grow densely in the epidermis of the dimorphic leaves. The present study examined the differentiation pattern of trichomes developing in the floating leaves of S. natans and S. molesta by scanning and transmission electron microscopy. Trichomes developing in the floating leaves of Salvinia showed very different patterns. In S. natans, they were arranged in a V-shape form, having 20${\sim}$25 rows at $18{\sim}25^{\circ}$ on both sides of the lamina divided by the midrib in the floating leaf. In each row, 8${\sim}$10 oval-shaped cells, $200{\sim}290{\mu}m$ in length, were arranged in a spiral fashion. Four trichomes of this form made a trichome unit, but their apical parts were separated from one another and developed into the so-called 'knuckle-crane' type. On the other hand, in S. molesta, trichomes differentiated in a unique pattern quite different from those of S. natans. At the early stage of differentiation, trichomes protruded from the epidermis and then 4${\sim}$6 cylindrical cells grew $400{\sim}600{\mu}m$ long and the four trichomes formed as an unit. The four grouped trichomes were interconnected through their apex and developed in the 'egg-beater' type. Then $300{\sim}600{\mu}m$ long multi-cellular stalk cells grew and protruded out of the epidermal surface from the basal part of the trichomes. Such a structural characteristic of trichomes is considered to play a very important role along with the aerenchyma tissue in the leaf mesophyll tissue for the floating of Salvinia on the water surface.

• Applied Microscopy
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• v.38 no.4
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• pp.279-284
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• 2008

The phase change materials have been extensively used as an optical rewritable data storage media utilizing their phase change properties. Recently, the phase change materials have been spotlighted for the application of non-volatile memory device, such as the phase change random access memory. In this work, we have investigated the crystallization behavior and microstructure analysis of In-Sb-Te (IST) thin films deposited by RF magnetron sputtering. Transmission electron microscopy measurement was carried out after the annealing at $300^{\circ}C$, $350^{\circ}C$, $400^{\circ}C$ and $450^{\circ}C$ for 5 min. It was observed that InSb phases change into $In_3SbTe_2$ phases and InTe phases as the temperature increases. It was found that the thickness of thin films was decreased and the grain size was increased by the bright field transmission electron microscopy (BF TEM) images and the selected area electron diffraction (SAED) patterns. In a high resolution transmission electron microscopy (HRTEM) study, it shows that $350^{\circ}C$-annealed InSb phases have {111} facet because the surface energy of a {111} close-packed plane is the lowest in FCC crystals. When the film was heated up to $400^{\circ}C$, $In_3SbTe_2$ grains have coherent micro-twins with {111} mirror plane, and they are healed annealing at $450^{\circ}C$. From the HRTEM, InTe phase separation was occurred in this stage. It can be found that $In_3SbTe_2$ forms in the crystallization process as composition of the film near stoichiometric composition, while InTe phase separation may take place as the composition deviates from $In_3SbTe_2$.

• Journal of the Korean Geotechnical Society
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• v.32 no.10
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• pp.41-53
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• 2016

Stability of the braced earth wall in the composite ground, which is composed of the jointed base rocks and the soil strata depends on the earth pressure acting on it. In most cases, the earth pressure is calculated by the empirical method, in which base rocks are considered as a soil strata with the shear strength parameters of base rocks. In this case the effect of the joint dips of the jointed base rocks is ignored. Therefore, the calculated earth pressure is smaller than the actual earth pressure. In this study, the magnitude and the distribution of the earth pressure acting on the braced wall in the composite ground depending on the joint dips of the base rocks and the ratio of soil strata and base rocks were experimentally studied. Two dimensional large-scale model tests were conducted in a large scale test facility (height 3.0 m, length 3.0 m and width 0.5 m) by installing 10 supports in a scale of 1/14.5. The test ground was presumed with the base rock ratio of the composite ground of 65%:35% and 50%:50% and with the joint dips for each base rock layer, $0^{\circ}$, $30^{\circ}$, $45^{\circ}$ and $60^{\circ}$, respectively. And then finite element analyses were performed in the same condition. As results, the earth pressure on the braced wall increased as the base rock layer's joint dips became larger. And earth pressure at the rock layer increased as the rock rate became larger. The largest earth pressure was measured when the base rock rate was 50% (R50) and the rock layer's joint dips was $60^{\circ}$. Based on these results, a formular for the calculation of the earth pressure in the composite ground could be suggested. Distribution of earth pressure was idealized in a quadrangular form, in which the magnitude and the position of peak earth pressure depended on the rock ratio and the joint dips.

• The Journal of Korean Academy of Prosthodontics
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• v.53 no.4
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• pp.310-317
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• 2015

Purpose: The purpose of this study was to evaluate the validity of reference points for edentulous patient by examining the correlation of teeth and face, and intraoral anatomic landmarks. Materials and methods: We examined a facial outline, length, bizygomatic width, nasion - gnathion length, glabella - nasion distance in 270 men and 280 women satisfied with inclusion criteria from Seoul National School of Dentistry. The shape of maxillary central incisor, mesiodistal crown width and length of maxillary 6 incisors, distance from incisive papilla to labial surface of maxillary central incisor, and perpendicular distance from incisive papilla to intercanine line were measured in the stone model. We analyzed the ratio and relevant relation statistically. Results: The probability on having the same shape of face and the relative same shape maxillary incisor was 55.56% and 46.43% for men and women. The facial length proved to be a more valuable measurement in women in the tooth selection. The ratio of bizygomatic width to mesiodistal width of maxillary central incisor, and the ratio of bizygomatic width to width of maxillary 6 incisors were 16.8 : 1 and 3.0 : 1 and were positively correlated with each other. The distance of the canines from the maxillary incisal papilla was $1.33{\pm}1.28mm$. The distance between the center of the incisal papilla and the labial surface of their maxillary central incisor was $9.23{\pm}1.20mm$. Conclusion: It was showed that anatomical reference points in tooth selection and arrangement for edentulous patient are useful and have validity in our limited study.

• Journal of the korean academy of Pediatric Dentistry
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• v.38 no.4
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• pp.337-347
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• 2011

The basic structure of the primary crowns usually resemble their succeeding permanent teeth. However, maxillary second primary molars resemble ipsilateral first permanent molars. Accordingly in this study, odontometric data of the two teeth was obtained, then analyzed to verify the morphological relationship and sex difference between the two teeth. Dental study casts were examined for their mesiodistal and buccolingual width of the crowns, diameter of each cusps, and intercuspal distances. Photographs of the crowns were taken to measure the angles between each cusp tip. The results are as follows : 1. In boys, PrI, PaI, DBC angle, and MBC angle did not show any statistically significant difference between the two teeth(p>0.05), and moderate level of correlations were observed. 2. In girls, crown index, DBC angle, and MBC angle of the two teeth did not show any statistically significant difference(p>0.05), and moderate level of correlations were recognized. 3. Measurements that did not show statistically significant difference between the two teeth in both boys and girls were DBC angle and MBC angle(p>0.05). In DLC angle, however, statistically significant difference was observed(p<0.01). 4. Most of the measurements showed sex differences, except DBC angle, which did not show any sex difference in both teeth(p>0.05).

• Solar Energy
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• v.20 no.2
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• pp.43-54
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• 2000

We prepared and characterized $Cu(In_{1-x}Ga_x)Se_2$(CIGS) films using a elemental co-evaporation method for absorbing layer of high efficiency thin film solar cells. The CIGS films deposited on a soda-lime glass exhibited low resistivity because of higher carrier concentration. Na was accumulated at the CIGS surface and the 0 and Se were also accumulated at the surface, suggesting that oxidation is a driving force of Na accumulation. The structure of CIGS film was modified or a secondary phase was formed in the Cu-poor CIGS bulk films probably due to the incorporation of Na into Cu vacancy sites. As the Ga/(In+Ga) ratio increased, the diffraction peaks of $Cu(In_{1-x}Ga_x)Se_2$ films were shifted to larger angle and splitted, and the grain size of $Cu_{0.91}(In_{1-x}Ga_x)Se_2$ films became smaller. All $Cu_{0.91}(In_{1-x}Ga_x)Se_2$ films showed the p-type conductivity regardless of the Ga/(In+Ga) ratio. Ag/n-ZnO/i-ZnO/CdS/$Cu_{0.91}(In_{0.7}Ga_{0.3})Se_2$/Mo solar cells were fabricated. The currently best efficiency in this study was 14.48% for $0.18cm^2$ area ($V_{oc}=581.5mV,\;J_{sc}=34.88mA$, F.F=0.714).