• Korean Journal of Plant Tissue Culture
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• v.26 no.3
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• pp.211-217
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• 1999

The effect of auxins and cytokinins on the formation of adventitious shoots, adventitious roots, trichomes, and calli in MS basal medium was investigated in leaf segments from ecotype Columbia of Arabidopsis thaliana. Adventitious shoots, adventitious roots, trichome, and calli were formed from leaf segments by a wide range of hormone concentrations and combinations. Adventitious shoots were formed respectively in treatment with 0.1mg/L IAA and 10 mg/L BA. Adventitious roots were formed in treatments with low concentration of IAA and NAA. Trichomes and calli were formed by increasing the concentration of IAA and NAA. The optimal combination was 0.5mg/L NAA and 0.1mg/L BA for trichome formation, 10mg/L NAA and 10mg/L BA for calli formation. When NAA was treated alone in culture media, adventitious roots were formed in 0.1mg/L, trichomes were formed in 2.0mg/L, and calli were formed in 10mg/L. Inductive time for formation of adventitious roots, trichomes and calli were determined at 6,7 and 18 days respectively by periodical transfer of leaf segments from NAA containing medium to NAA free medium.

• Journal of The Korean Society of Grassland and Forage Science
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• v.29 no.4
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• pp.285-290
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• 2009

A suitable system for plant regeneration has been established for perennial ryegrass (Lolium perenne L.). In order to investigate the effects of genetic variations of perennial ryegrass in tissue culture response, calli were induced from mature seeds of five cultivars, 'Topgun', 'Accent', 'Renenge GLX', 'Tetrellite', 'Bison' and plant regeneration frequency was compared. Significant differences were observed among the cultivars in both callus induction and plant regeneration. Genotype 'Accent' consistently performed best in the callus formation and plant regeneration. These results can be used useful for molecular breeding of perennial ryegrass through genetic transformation.

• Korean Journal of Plant Resources
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• v.17 no.2
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• pp.153-160
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• 2004

Glehnia littoralis is known as an edible and medicinal plant using green loaves and mature roots of plant. In the present paper, the influence of plant growth regulators on callus induction and plant regeneration was investigated. Callus induction and regeneration occurred from leaf and petiole explants in Glehnia littoralis. Optimal condition of plant growth regulators for callus induction from leaf and petiole explants was MS basal medium supplemented with 2mg/L 2,4-D and 2mg/L BA. The frequency of callus induction was higher in petiole explant than leaf. When the callus was cultured on MS basal medium supplemented with 0∼1 mg/L IAA, 0∼1mg/L NAA and 0∼2mg/L BA for about 65 days, the most effective plant growth regulators on plant regeneration from callus were 1mg/L NAA and 2mg/L BA. The plantlets acclimatized successfully and grown in vermiculite matrix.

• Journal of Environmental Science International
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• v.3 no.2
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• pp.141-155
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• 1994

The effect of growth regulators (NAA, BA and $ extrm{GA}_3$) and light (blue, red and far-red) on the changes in total protein and thylakoid membrane protein pattern of callus from intergeneric protoplast fusion between Nicotiana tabacum and Solanum nigrw were investigated. When the callus were irradiated with different wavelengths of light, blue and red light accelerated the synthesis of total proteins and thylakoid membrane proteins. Particularly, red light led to an increase in the protein synthesis compared to blue light. When the callus were subjected to various combinations of growth regulators, NAA+$ extrm{GA}_3$ and NAA+BA treatments induced remarkable increase of total proteins and thylakoid membrane proteins accumulation, particularly in the combination of NAA+$ extrm{GA}_3$. NAA.$ extrm{GA}_3$ treatment with irradiation of red ligh showed highest value in the accumulation of total proteins and thylakoid membrane proteins. We conclude that simultaneous application of red light and NAA+$ extrm{GA}_3$ treatment may induce synergistic effect in the synthesis of total proteins and thylakoid membrane Proteins.

• Korean Journal of Plant Tissue Culture
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• v.21 no.5
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• pp.281-286
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• 1994

The hormonal regulation of organ differentiation was investigated in the tissue culture of sweet potato. Embryogenic callus was induced from shoot tips cultured on MS medium supplemented with 1 mg/L 2,4-D. When embryogenic callus was transferred to medium containing 0.1 mg/L GA$_4$, it proliferation was stimulated. The callus gave rise to plantlets when cultured on medium containing 0.1 mg/L BA. Addition of 0.1 mg/L jasmonic acid or 0.01 mg/L brassinolide to medium was effective for the development of healthy normal plantlets.

• Journal of The Korean Society of Grassland and Forage Science
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• v.31 no.3
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• pp.211-216
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• 2011

The present study was conducted to determine the optimum in vitro conditions for callus induction and plant regeneration from mature seed derived callus of four cultivars of Timothy. In order to investigate the effects of genetic variations of timothy in tissue culture, calli were induced from mature seeds of four varieties, 'Colt', 'Chair', 'Richmond' and 'Hokuo' and plant regeneration frequency was compared. Significant differences were observed among the cultivars in both callus induction and plant regeneration. Genotype 'Colt' consistently performed best in the callus subculture and plant regeneration. The complete plantlets were thereafter transplanted to grow under greenhouse condition. Regenerated timothy plants were morphologically uniform with normal leaf and growth pattern.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.04a
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• pp.79-79
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• 2019

홍띠(Imperata cylindrica 'Rubra') 식물자원의 기내대량증식과 재분화식물체의 순화체계를 구축하고자 기내 재분화에 적합한 식물재료부위, 생장조절물질을 조사하고, 재분화 유식물체로부터 적정 순화조건을 구명하였다. 기본배지로 MS (Murashige and Skoog, 1962) 배지를 사용하였고, 배양은 $26{\times}2^{\circ}C$, $25{\mu}mol/m^2/s$, 14h/10h (day/night) 광조건 하의 배양실에서 수행하였다. 캘러스 형성은 뿌리 끝, 줄기절편, 생장점 부위 중생장점 부위에서 가장 양호하였고, 이 생장점 조직에 0.1 mg/L의 2,4-D와 2 mg/L의 BA를 처리하였을 때 양호하였다. 캘러스 증식은 0.1 mg/L의 2,4-D와 0.05 mg/L의 BA 배지, 0.05 mg/L의 2,4-D와 0.5 mg/L의 BA를 첨가한 배지 중 0.1 mg/L의 2,4-D와 0.05 mg/L의 BA 배지에서 양호하였고, 이들 캘러스로부터 신초 재분화는 0.01 mg/L의 NAA와 2 mg/L의 BA 처리에서 양호하였다. 초기 치상으로부터 실제 경과시간은 캘러스 유도에 19주간(2018. 03. 18~07. 27), 캘러스 증식 9주간(2018. 07. 27~09. 28), 신초 유도 11주간(2018. 09.28~12. 14), 순화에 10주간(2018. 12. 14~2019. 02. 23)에 걸쳐 진행하였으나 확립된 배양계를 적용하면 캘러스 유도 4주, 캘러스 증식 3주, 신초유도 및 증식 ４주, 순화 ７주 정도가 소요될 것으로 계측되었다. 순화는 다경줄기 형성후 MS배지를 멸균한 상토(버미큘라이트) 또는 종이포트로 교체하여 재분화식물체를 배양병에서 7주간 배양하고, 7주후에 배양병 뚜껑을 1/10 정도 1차 개방하여 1주일 후 3/10 정도 개방하여 2주간 경과한 후 컵포트(직경 6 cm)에 이식하여 성공적으로 활착시켰다.

• Korean Journal of Plant Resources
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• v.35 no.5
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• pp.652-658
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• 2022

This study was conducted to induce somatic embryogenic callus (SEC) derived from petals in rose. The petal explants of 3 cultivars ('Ice Wing', 'Orange Eye' and 'Pink Beauty') with different flower colors were placed on three types media (MS, SH and WPM) supplemented with 11 mg/L 2,4-D, respectively, and then cultured in the dark for 47 days. Calluses were formed at explants of all three cultivars. Also, 'Ice Wing', which were cultured in the SH as the basal medium, showed the highest callus formation rate. However, somatic embryos were generated from only petal-derived callus of 'Ice Wing', which were induced on the WPM as the basal medium, transferred it to SH basal medium supplemented with 3 mg/L 2,4-D, and 300 mg/L L-proline, and cultured for 5 weeks. The SEC has been proliferated every four weeks at the subculture interval. In addition, as a results of making a comparison of expression of RhSERK3 and RhSERK4, which is used as signal for generation of somatic embryo from callus in rose, between the SEC and petal-derived callus from 'Ice Wing' by RT-qPCR, the former showed 10 times higher RhSERK3 expression and 700 times higher RhSERK4 expression than the latter.

• Journal of Plant Biotechnology
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• v.42 no.4
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• pp.396-400
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• 2015

To investigate the optimal conditions for shoot organogenesis in Astragalus sinicus L., hypocotyl explants were cultured in Murashige & Skoog's (MS) medium supplemented with 0.1, 1.0, 2.0, or 4.0 mg/L 2,4-dichlorophenoxy acetic acid (2,4-D) for 6 weeks. 2,4-D concentration significantly effected morphogenesis: some produced calli with adventitious shoots and roots, some produced calli with adventitious roots, some produced only calli, and some produced deep-brownish calli with roots. The formation of calli with shoots and/or roots was observed at lower levels of 2,4-D, whereas calli without shoots or with deep-brownish roots were formed after treatment with higher levels of 2,4-D. Also, a shoot organogenesis ability of callus clones was observed after treatment with medium with 0.1 or 1.0 mg/L 2,4-D grown in MS medium with combinations of benzyl adenine (BA) and 2,4-D for 4 weeks. Medium with a combination of BA and 2,4-D was effective for shoot formation, whereas root organogenesis from calli decreased. The greatest amount of shoot formation was obtained when calli were cultured in MS medium containing 1.0 mg/L 2,4-D and 0.5 mg/L BA. Upon shoot transfer into 1/2 MS basal medium, plantlets developed, and the plantlets grew well in soil in a greenhouse.

• Journal of The Korean Society of Grassland and Forage Science
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• v.26 no.2
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• pp.69-76
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• 2006

To optimize tissue culture responses for genetic transformation of Kentucky bluegrass, the effects of culture medium supplements on tissue culture responses were investigated with mature seeds of a cultivar 'Newport' as explant tissues. The optimal concentration of 2,4-D (2.4-dichloro phenoxy acetic acid) for the induction of embryogenic callus from mature seed was 3 mg/L. Plant regeneration frequency was 54% when embryogenic callus was cultured on the regeneration medium supplemented with 1 mg/L 2,4-D and 3 mg/L of BA (6-benzyladenine). Addition of 1 g/L of casein hydrolysate and 500 mg/L of L-proline improved frequencies of embryogenic callus induction and plant regeneration up to 60.8% and 58.3%, respectively. Regenerated plants were grown normally when shoots transplanted to the soil. A rapid and efficient plant regeneration system established in this study. We suggest that the results may be useful for molecular breeding of Kentucky bluegrass through genetic transformation.