Journal of The Korean Society of Grassland and Forage Science (한국초지조사료학회지)

The Korean Society of Grassland and Forage Science (한국초지조사료학회)
권호 표지
DOI QR코드

DOI QR Code

Plant Regeneration Capacity of Calluses Derived from Mature Seed of Perennial Ryegrass Cultivars

페레니얼 라이그라스의 품종에 따른 성숙종자 유래의 캘러스로부터 식물체 재분화

  • 이기원 (농촌진흥청 국립축산과학원) ;
  • 이종경 (농촌진흥청 국립축산과학원) ;
  • 김기용 (농촌진흥청 국립축산과학원) ;
  • 지희정 (농촌진흥청 국립축산과학원) ;
  • 박형수 (농촌진흥청 국립축산과학원) ;
  • 김경희 (경상대학교 응용생명과학부) ;
  • 이병현 (경상대학교 응용생명과학부) ;
  • 이상훈 (농촌진흥청 국립축산과학원)
  • Published : 2009.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

A suitable system for plant regeneration has been established for perennial ryegrass (Lolium perenne L.). In order to investigate the effects of genetic variations of perennial ryegrass in tissue culture response, calli were induced from mature seeds of five cultivars, 'Topgun', 'Accent', 'Renenge GLX', 'Tetrellite', 'Bison' and plant regeneration frequency was compared. Significant differences were observed among the cultivars in both callus induction and plant regeneration. Genotype 'Accent' consistently performed best in the callus formation and plant regeneration. These results can be used useful for molecular breeding of perennial ryegrass through genetic transformation.

페레니얼 라이그라스의 5가지 품종을 이용하여 성숙종자로부터 배발생 캘러스 유도 및 캘러스로부터의 식물체 재분화 효율을 조사하였다. 성숙종자로부터 4주 동안 배양한 캘러스 유도율은 'Accent', 'Topgun', 'Renenge GLX', 'Bison', 'Tetrellite' 순으로 높은 캘러스 유도율을 보였고 암상태에서 배양한 캘러스가 광상태에서 배양한 것보다 7~9% 정도 높은 효율을 나타내었다. 식물체로의 재분화에 적합한 배지는 MS 배지보다 N6 배지에서 식물체 재분화율이 우수하였다. 품종간의 재분화율은 'Bison', 'Accent', 'Topgun', 'Renenge GLX', 'Tetrellite' 순으로 높게 나타났으며 광상태에서 유식물로 분화되는 개체가 1~7% 정도 많이 관찰되었다. 본 연구를 통하여 밝혀진 캘러스 유도율과 식물체 재분화율이 우수한 품종은 분자육종을 통한 신품종 페레니얼 라이그라스의 개발에 유용하게 이용될 수 있을 것이다.

Keywords

References

  1. Altpeter, F. and J. Xu. 2000. Rapid production of transgenic turfgrass (Festuca rubra L.) plants. Plant Physiol. 157:441-448 https://doi.org/10.1016/S0176-1617(00)80029-2
  2. Chen, L., C.K. Auh, P. Dowling, J. Bell, F. Chen, A. Hopkins, R.A. Dixon and Z.Y. Wang. 2003. Improved forage digestibility of tall fescue (Festuca arundinacea) by transgenic downregulation of cinnamyl alcohol dehydrogenase. Plant Biotechnol. J. 1:437-449 https://doi.org/10.1046/j.1467-7652.2003.00040.x
  3. Chu, C.C., C.S. Wang, C.C. Sun, C. Hsu, K.C. Yin, C.Y. Chu and F.Y. Bi. 1975. Establishment of an efficient medium for anther culture of rice through comparative experiments on the nitrogen sources. Scienta Sinic. 18:659-668
  4. Forster, J.W and G. Spangenberg. 1999. Forage and turf grass biotechnology: principles, methods and prospects. In: Setlow, J. K. (Ed.), Genetic engineering: principles and methods, Vol. 21. Kluwer Academic Publishers. New York, p. 191
  5. Lee, K.-W., D.-G. Lee, N. Ahsan, S.-H. Won, S.-H. Lee, K.-Y. Kim, G.J. Choi, S. Seo and B.-H. Lee. 2007. Effect of plant growth regulators on callus induction and plant regeneration of perennial ryegrass (Lolium perenne L.). J. Korean Grassl. Sci. 27:235-240 https://doi.org/10.5333/KGFS.2007.27.4.235
  6. Lee, K.-W., S.-H. Lee, D.-G. Lee, H.-S. Lee, M.S. Choi, K.Y. Kim, H.S. Lee and B.H. Lee. 2005. Effect of plant growth regulators and antioxidants on callus induction and plant regeneration from seed culture of orchardgrass. J. korean Grassl. Sci. 25:191-198 https://doi.org/10.5333/KGFS.2005.25.3.191
  7. Lee, S.-H., D.-G. Lee, J.-S. Kim and B.-H. Lee. 2003. High-frequency plant regeneration from mature seed-derived callus culture of orchardgrass. J. Korean Plant Biotech. 30:341-346 https://doi.org/10.5010/JPB.2003.30.4.341
  8. Murashige, T. and F. Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Plant Physiol. 15:473-497 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  9. Rim, Y.W., K.Y. Kim, K.J. Choi, B.R. Sung and J.S. Shin. 2000. Callus induction from seeds of Italian ryegrass and plant regeneration. J Kor Grassland Sci. 20:25-30
  10. Sato, H. and T. Takamizo. 2006. Agrobacterium tumefaciens-mediated transformation of forage-type perennial ryegrass (Lolium perenne L.). Grassland Sci. 52:95-98 https://doi.org/10.1111/j.1744-697X.2006.00053.x
  11. Somers, D. A., D. A. Samac and P.M. Olhoft. 2003. Recent advances in legume transformation. Plant Physiol. 131:892-899 https://doi.org/10.1104/pp.102.017681
  12. Spangenberg, G., Z.Y. Wang and I. Potrykus. 1998. Biotechnology in forage and turf grass improvement. In: Frankel et al. (Eds), Monographs on theoretical and applied genetics, Vol. 23, Springer Verlag, Heidelberg. p. 192
  13. Spangenberg, G., Z.Y. Wang, X.L. Wu, J. Nagel and I Potrykus. 1995. Transgenic perennial ryegrass (Lolium perenne) plants from microprojectile bombardment of embryogenic suspension cells, Plant Sci. 108:pp. 209-217 https://doi.org/10.1016/0168-9452(95)04135-H
  14. Vasil, V. and I.K. Vasil. 1984. Induction and maintanance of embryogenic callus cultures of Gramineae. In: Vasil IK(eds), Cell culture and somatic cell genetics of plants, Vol 1, Academic Press, Orlando. pp. 36-42
  15. Wang, Z.Y., J. Nagel, I. Potrykus and G. Spangenberg. 1993. Plants from cell suspensionderived protoplasts in Lolium species. Plant Sci. 94:179-193
  16. Wu, Y.-Y., Q.-J. Chen, M. Chen, J. Chen and X.-C. Wang. 2005. Salt-tolerant transgenic perennial ryegrass (Lolium perenne L.) obtained by Agrobacterium tumefaciens-mediated transformation of the vacuolar $Na^+/H^+$ antiporter gene. Plant Sci. 169:165-173 https://doi.org/10.1016/j.plantsci.2005.03.011